Restriction map for
pFN21AA0460
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AviII
BbeI BbrPI BbvCI BfrI BsaBI
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII BssSI
BstAPI ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoRV FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI
PacI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 3085 CGCG CG^CG
AccIII 86 TCCGGA T^CCGGA
AgeI 2666 ACCGGT A^CCGGT
AocI 3547 CCTAAGG CC^T[ACGT]AGG
Aor51HI 491 AGCGCT AGC^GCT
AspI 1476 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4156 CCTAGG C^CTAGG
BalI 2967 TGGCCA TGG^CCA
BamHI 225 GGATCC G^GATCC
BbsI 994 GAAGAC GAAGAC(2/6)
BcgI 2030 GCACCTCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1081 GTATCC GTATCC(6/5)
BclI 3045 TGATCA T^GATCA
BcnI 4139 CCCGG CC^[GC]GG
BglII 91 AGATCT A^GATCT
BlnI 4156 CCTAGG C^CTAGG
BlpI 3898 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3898 GCTGAGC GC^T[ACGT]AGC
BpuAI 994 GAAGAC GAAGAC(2/6)
BsaAI 2939 CACGTA [CT]AC^GT[GA]
BseAI 86 TCCGGA T^CCGGA
BsmI 1448 GCATTC GAATGC(1/-1)
BspEI 86 TCCGGA T^CCGGA
BspHI 1286 TCATGA T^CATGA
BsrBI 2834 CCGCTC CCGCTC(-3/-3)
BsrFI 2666 ACCGGT [GA]^CCGG[CT]
Bst1107I 283 GTATAC GTA^TAC
BstBI 3609 TTCGAA TT^CGAA
BstUI 3085 CGCG CG^CG
BstZ17I 283 GTATAC GTA^TAC
Bsu36I 3547 CCTAAGG CC^T[ACGT]AGG
CelII 3898 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2666 ACCGGT [GA]^CCGG[CT]
DraIII 3832 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 491 AGCGCT AGC^GCT
Eco81I 3547 CCTAAGG CC^T[ACGT]AGG
EcoRI 3680 GAATTC G^AATTC
EcoT22I 758 ATGCAT ATGCA^T
FauI 3083 CCCGC CCCGC(4/6)
FbaI 3045 TGATCA T^GATCA
MluNI 2967 TGGCCA TGG^CCA
MroI 86 TCCGGA T^CCGGA
MscI 2967 TGGCCA TGG^CCA
MslI 3244 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 3085 CGCG CG^CG
NciI 4139 CCCGG CC^[GC]GG
NsiI 758 ATGCAT ATGCA^T
NspV 3609 TTCGAA TT^CGAA
PaeR7I 3572 CTCGAG C^TCGAG
PflFI 1476 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2666 ACCGGT A^CCGGT
PstI 1064 CTGCAG CTGCA^G
RcaI 1286 TCATGA T^CATGA
SacI 2230 GAGCTC GAGCT^C
SalI 2901 GTCGAC G^TCGAC
ScaI 55 AGTACT AGT^ACT
SfuI 3609 TTCGAA TT^CGAA
Tth111I 1476 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3572 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 283 GTATAC GT^[AC][GT]AC
2901 GTCGAC
AvaI 2225 CCCGAG C^[CT]CG[GA]G
3572 CTCGAG
BanI 2629 GGTGCC G^G[CT][GA]CC
3399 GGCACC
BsaWI 86 TCCGGA [AT]^CCGG[AT]
2666 ACCGGT
BsmBI 2602 CGTCTC CGTCTC(1/5)
3087 CGTCTC
BsoBI 2225 CCCGAG C^[CT]CG[GA]G
3572 CTCGAG
BstEII 2669 GGTCACC G^GT[ACGT]ACC
2845 GGTCACC
BstPI 2669 GGTCACC G^GT[ACGT]ACC
2845 GGTCACC
BstXI 1766 CCAACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2529 CCAAGAATTTGG
CfoI 492 GCGC GCG^C
1982 GCGC
DrdI 2258 GACCCTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3201 GACCGTAGAGTC
EaeI 2967 TGGCCA [CT]^GGCC[GA]
4141 CGGCCA
EcoNI 1321 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1419 CCTCTCCAAGG
EcoO65I 2669 GGTCACC G^GT[ACGT]ACC
2845 GGTCACC
HaeII 491 AGCGCT [GA]GCGC^[CT]
1981 AGCGCC
HgaI 868 GACGC GACGC(5/10)
3086 GCGTC
HhaI 492 GCGC GCG^C
1982 GCGC
HinP1I 492 GCGC G^CGC
1982 GCGC
HindIII 317 AAGCTT A^AGCTT
1653 AAGCTT
MfeI 811 CAATTG C^AATTG
1718 CAATTG
MunI 811 CAATTG C^AATTG
1718 CAATTG
NcoI 938 CCATGG C^CATGG
4035 CCATGG
SapI 472 GAAGAGC GCTCTTC(1/4)
3061 GAAGAGC
SmlI 427 CTCAAG C^T[CT][GA]AG
3572 CTCGAG
XcmI 1853 CCAAGCCAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2855 CCACGTCAGGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AspHI
AvaII BanII BbvI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI