(Circular) (Six-base) MAPSORT of: pFN21AA0460.seq Check: 3730 from: 1 to: 8977 pFN21AA0460 8977 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2662 3388 3394 2662 Size: 726 6 8245 Fragments arranged by size: 8245 726 6 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6787 278 Size: 53 83 186 936 5251 2468 Fragments arranged by size: 5251 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2289 4907 6320 1937 Size: 352 2618 1413 4594 Fragments arranged by size: 4594 2618 1413 352 Acc65I G'GTAC_C Cuts at: 6298 6298 Size: 8977 AclI AA'CG_TT Cuts at: 1563 7104 7477 1563 Size: 5541 373 3063 Fragments arranged by size: 5541 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 2540 2862 3506 3561 3690 3711 3831 Size: 597 311 322 644 55 129 21 120 Cuts at: 3831 3846 4624 7040 8424 8692 1632 Size: 15 778 2416 1384 268 1917 Fragments arranged by size: 2416 1917 1384 778 644 597 322 311 268 129 120 55 21 15 AfeI AGC'GCT Cuts at: 2498 2498 Size: 8977 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8780 AflIII A'CryG_T Cuts at: 1203 7876 8853 1203 Size: 6673 977 1327 Fragments arranged by size: 6673 1327 977 AgeI A'CCGG_T Cuts at: 4671 7808 4671 Size: 3137 5840 AhdI GACnn_n'nnGTC Cuts at: 7706 7706 Size: 8977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8977 AlwNI CAG_nnn'CTG Cuts at: 2840 3152 3494 5852 6720 8292 2840 Size: 312 342 2358 868 1572 3525 Fragments arranged by size: 3525 2358 1572 868 342 312 ApaI G_GGCC'C Cuts at: 4579 5143 5948 6035 6233 4579 Size: 564 805 87 198 7323 Fragments arranged by size: 7323 805 564 198 87 ApaLI G'TGCA_C Cuts at: 7034 8190 7034 Size: 1156 7821 ApoI r'AATT_y Cuts at: 1493 2453 2618 2648 2718 4538 4916 5685 6286 Size: 960 165 30 70 1820 378 769 601 Cuts at: 6286 6512 6664 8773 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1820 1697 960 769 601 378 226 165 152 70 30 AseI AT'TA_AT Cuts at: 160 1032 7531 160 Size: 872 6499 1606 Fragments arranged by size: 6499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8977 AvaI C'yCGr_G Cuts at: 1870 1943 4230 5577 6302 1870 Size: 73 2287 1347 725 4545 Fragments arranged by size: 4545 2287 1347 725 73 AvrII C'CTAG_G Cuts at: 6161 6161 Size: 8977 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3081 3114 3081 Size: 33 8944 BamHI G'GATC_C Cuts at: 2230 6307 6674 2230 Size: 4077 367 4533 Fragments arranged by size: 4533 4077 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4634 5404 6298 7753 618 Size: 325 217 627 2847 770 894 1455 1842 Fragments arranged by size: 2847 1842 1455 894 770 627 325 217 BanII G_rGCy'C Cuts at: 1391 3744 4239 4534 4579 5143 5948 6035 6233 Size: 2353 495 295 45 564 805 87 198 Cuts at: 6233 6296 1391 Size: 63 4072 Fragments arranged by size: 4072 2353 805 564 495 295 198 87 63 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3006 928 Size: 971 1107 6899 Fragments arranged by size: 6899 1107 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4024 4058 7168 7202 1903 Size: 34 2087 34 3110 34 3678 Fragments arranged by size: 3678 3110 2087 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3097 6869 8079 3097 Size: 3772 1210 3995 Fragments arranged by size: 3995 3772 1210 BclI T'GATC_A Cuts at: 1548 5050 1548 Size: 3502 5475 BfrBI ATG'CAT Cuts at: 2765 2765 Size: 8977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3716 5373 5524 Size: 107 122 71 631 605 2044 1657 151 Cuts at: 5524 7588 136 Size: 2064 1525 Fragments arranged by size: 2064 2044 1657 1525 631 605 151 122 107 71 BglII A'GATC_T Cuts at: 2096 8972 2096 Size: 6876 2101 BlpI GC'TnA_GC Cuts at: 5904 6394 5904 Size: 490 8487 Bme1580I G_kGCm'C Cuts at: 1790 3640 4579 4994 5143 5407 5948 6035 6233 Size: 1850 939 415 149 264 541 87 198 Cuts at: 6233 7038 8194 1790 Size: 805 1156 2573 Fragments arranged by size: 2573 1850 1156 939 805 541 415 264 198 149 87 BmgBI CAC'GTC Cuts at: 1318 4863 4905 1318 Size: 3545 42 5390 Fragments arranged by size: 5390 3545 42 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2926 3140 3312 Size: 416 53 83 399 24 1509 214 172 Cuts at: 3312 3536 3543 4758 4790 6708 7666 442 Size: 224 7 1215 32 1918 958 1753 Fragments arranged by size: 1918 1753 1509 1215 958 416 399 224 214 172 83 53 32 24 7 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2650 3901 6014 2650 Size: 1251 2113 5613 Fragments arranged by size: 5613 2113 1251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3315 3616 3890 5691 5820 7637 1239 Size: 2076 301 274 1801 129 1817 2579 Fragments arranged by size: 2579 2076 1817 1801 301 274 129 Bpu10I CC'TnA_GC Cuts at: 3272 3968 5978 3272 Size: 696 2010 6271 Fragments arranged by size: 6271 2010 696 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2417 7102 7967 8265 2417 Size: 4685 865 298 3129 Fragments arranged by size: 4685 3129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3859 4312 6177 6199 7640 882 Size: 617 2360 453 1865 22 1441 2219 Fragments arranged by size: 2360 2219 1865 1441 617 453 22 BsaAI yAC'GTr Cuts at: 493 1204 4946 6768 493 Size: 711 3742 1822 2702 Fragments arranged by size: 3742 2702 1822 711 BsaBI GATnn'nnATC Cuts at: 8971 8971 Size: 8977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6784 7166 7801 275 Size: 53 83 186 936 5251 382 635 1451 Fragments arranged by size: 5251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2091 4671 7410 7808 8082 8229 8640 2091 Size: 2580 2739 398 274 147 411 2428 Fragments arranged by size: 2739 2580 2428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2864 2894 3154 3184 3863 Size: 30 165 30 1461 30 260 30 679 Cuts at: 3863 3893 5689 5719 1178 Size: 30 1796 30 4436 Fragments arranged by size: 4436 1796 1461 679 260 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4779 5086 5089 5655 5697 5700 5766 6060 Size: 3593 307 3 566 42 3 66 294 Cuts at: 6060 6063 1186 Size: 3 4100 Fragments arranged by size: 4100 3593 566 307 294 66 42 3 3 3 BseYI C'CCAG_C Cuts at: 3214 3350 4184 4982 5033 5815 8180 8676 3214 Size: 136 834 798 51 782 2365 496 3515 Fragments arranged by size: 3515 2365 834 798 782 496 136 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2578 3366 3447 3480 1163 Size: 573 842 788 81 33 6660 Fragments arranged by size: 6660 842 788 573 81 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6427 7188 8216 1540 Size: 275 186 4426 761 1028 2301 Fragments arranged by size: 4426 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3575 4239 5073 5291 5679 5838 7038 7123 8194 Size: 664 834 218 388 159 1200 85 1071 Cuts at: 8194 3575 Size: 4358 Fragments arranged by size: 4358 1200 1071 834 664 388 218 159 85 BsmI GAATG_Cn' Cuts at: 3453 6488 6581 3453 Size: 3035 93 5849 Fragments arranged by size: 5849 3035 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4613 5098 4613 Size: 485 8492 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3575 3640 3744 4239 4534 4579 4994 Size: 399 1785 65 104 495 295 45 415 Cuts at: 4994 5073 5143 5291 5407 5679 5838 5948 6035 Size: 79 70 148 116 272 159 110 87 Cuts at: 6035 6233 6296 7038 7123 8194 1391 Size: 198 63 742 85 1071 2174 Fragments arranged by size: 2174 1785 1071 742 495 415 399 295 272 198 159 148 116 110 104 87 85 79 70 65 63 45 BspEI T'CCGG_A Cuts at: 2091 8640 2091 Size: 6549 2428 BspHI T'CATG_A Cuts at: 3291 6866 8596 3291 Size: 3575 1730 3672 Fragments arranged by size: 3672 3575 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2662 3076 3388 3394 6332 844 Size: 1818 414 312 6 2938 3489 Fragments arranged by size: 3489 2938 1818 414 312 6 BsrBI CCG'CTC Cuts at: 1394 1823 4841 6864 1394 Size: 429 3018 2023 3507 Fragments arranged by size: 3507 3018 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7472 7646 65 Size: 1183 6224 174 1396 Fragments arranged by size: 6224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4671 7621 7808 1700 Size: 110 2861 2950 187 2869 Fragments arranged by size: 2950 2869 2861 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8977 BssSI C'ACGA_G Cuts at: 7037 8049 7037 Size: 1012 7965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8977 BstBI TT'CG_AA Cuts at: 5615 7781 5615 Size: 2166 6811 BstEII G'GTnAC_C Cuts at: 1184 4674 4850 1184 Size: 3490 176 5311 Fragments arranged by size: 5311 3490 176 BstXI CCAn_nnnn'nTGG Cuts at: 3778 4541 3778 Size: 763 8214 BstYI r'GATC_y Cuts at: 1972 2096 2230 2836 3247 5608 6307 6674 7059 Size: 124 134 606 411 2361 699 367 385 Cuts at: 7059 7076 8528 8614 8626 8972 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2361 1977 1452 699 606 411 385 367 346 134 124 86 17 12 BstZ17I GTA'TAC Cuts at: 2290 2290 Size: 8977 Bsu36I CC'TnA_GG Cuts at: 5553 5553 Size: 8977 BtgI C'CryG_G Cuts at: 513 1065 2004 2943 4845 5938 6040 513 Size: 552 939 939 1902 1093 102 3450 Fragments arranged by size: 3450 1902 1093 939 939 552 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8238 Fragments arranged by size: 8238 683 56 BtsI GCAGTG_nn' Cuts at: 2396 3497 4135 4285 5743 6082 6493 7287 7307 Size: 1101 638 150 1458 339 411 794 20 Cuts at: 7307 2396 Size: 4066 Fragments arranged by size: 4066 1458 1101 794 638 411 339 150 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8942 DraI TTT'AAA Cuts at: 2266 2799 3760 5380 6201 6281 2266 Size: 533 961 1620 821 80 4962 Fragments arranged by size: 4962 1620 961 821 533 80 DraIII CAC_nnn'GTG Cuts at: 5842 5842 Size: 8977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4269 5212 7984 809 Size: 507 2953 943 2772 1802 Fragments arranged by size: 2953 2772 1802 943 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4972 6146 6424 Size: 54 1425 50 275 18 3142 1174 278 Cuts at: 6424 7313 8942 8 Size: 889 1629 43 Fragments arranged by size: 3142 1629 1425 1174 889 278 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6424 1537 Size: 275 4612 4090 Fragments arranged by size: 4612 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2472 2619 3139 3183 3703 4345 5061 6907 Size: 1119 147 520 44 520 642 716 1846 Cuts at: 6907 1353 Size: 3423 Fragments arranged by size: 3423 1846 1119 716 642 520 520 147 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7564 7938 8084 387 Size: 1064 138 5975 374 146 1280 Fragments arranged by size: 5975 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4237 4237 Size: 8977 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 2540 2862 3315 3506 3561 3616 Size: 393 597 311 322 453 191 55 55 Cuts at: 3616 3690 3711 3831 3846 3890 4624 5691 5820 Size: 74 21 120 15 44 734 1067 129 Cuts at: 5820 7040 7637 8424 8692 1239 Size: 1220 597 787 268 1524 Fragments arranged by size: 1524 1220 1067 787 734 597 597 453 393 322 311 268 191 129 120 74 55 55 44 21 15 EcoNI CCTnn'n_nnAGG Cuts at: 3330 3428 3330 Size: 98 8879 EcoO109I rG'GnC_Cy Cuts at: 2467 4412 4575 4576 5139 5140 5895 5927 5944 Size: 1945 163 1 563 1 755 32 17 Cuts at: 5944 5945 5959 6092 6229 6230 2467 Size: 1 14 133 137 1 5214 Fragments arranged by size: 5214 1945 755 563 163 137 133 32 17 14 1 1 1 1 EcoRI G'AATT_C Cuts at: 5685 6286 6664 5685 Size: 601 378 7998 Fragments arranged by size: 7998 601 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3771 4573 3771 Size: 802 8175 FspI TGC'GCA Cuts at: 7483 7483 Size: 8977 HaeII r_GCGC'y Cuts at: 1678 2500 3990 8124 1678 Size: 822 1490 4134 2531 Fragments arranged by size: 4134 2531 1490 822 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2864 2896 2971 3003 3017 Size: 32 135 32 1292 32 75 32 14 Cuts at: 3017 3049 3154 3186 3385 3417 3863 3895 4127 Size: 32 105 32 199 32 446 32 232 Cuts at: 4127 4159 4307 4339 4595 4627 5324 5356 6096 Size: 32 148 32 256 32 697 32 740 Cuts at: 6096 6128 7618 7650 7692 7724 1373 Size: 32 1490 32 42 32 2626 Fragments arranged by size: 2626 1490 1292 740 697 446 256 232 199 148 135 105 75 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 2538 2826 4908 5791 6321 6567 Size: 988 281 600 288 2082 883 530 246 Cuts at: 6567 669 Size: 3079 Fragments arranged by size: 3079 2082 988 883 600 530 288 281 246 HindIII A'AGCT_T Cuts at: 748 2322 3658 748 Size: 1574 1336 6067 Fragments arranged by size: 6067 1574 1336 HpaI GTT'AAC Cuts at: 6567 6567 Size: 8977 KpnI G_GTAC'C Cuts at: 6302 6302 Size: 8977 MfeI C'AATT_G Cuts at: 2816 3723 6576 2816 Size: 907 2853 5217 Fragments arranged by size: 5217 2853 907 MluI A'CGCG_T Cuts at: 8853 8853 Size: 8977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2790 3795 4743 5466 8091 8275 1305 Size: 1485 1005 948 723 2625 184 2007 Fragments arranged by size: 2625 2007 1485 1005 948 723 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4974 10 Size: 54 1425 343 3142 4013 Fragments arranged by size: 4013 3142 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5253 6935 7294 7453 518 Size: 648 4087 1682 359 159 2042 Fragments arranged by size: 4087 2042 1682 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2216 2356 3083 5361 6393 7070 8218 8463 Size: 610 140 727 2278 1032 677 1148 245 Cuts at: 8463 1606 Size: 2120 Fragments arranged by size: 2278 2120 1148 1032 727 677 610 245 140 NcoI C'CATG_G Cuts at: 513 1065 2004 2943 6040 513 Size: 552 939 939 3097 3450 Fragments arranged by size: 3450 3097 939 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8977 NotI GC'GGCC_GC Cuts at: 6424 6424 Size: 8977 NsiI A_TGCA'T Cuts at: 2767 2767 Size: 8977 NspI r_CATG'y Cuts at: 1132 1229 6335 7880 1132 Size: 97 5106 1545 2229 Fragments arranged by size: 5106 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3737 4208 4622 5931 1348 Size: 2389 471 414 1309 4394 Fragments arranged by size: 4394 2389 1309 471 414 PciI A'CATG_T Cuts at: 7876 7876 Size: 8977 PflMI CCAn_nnn'nTGG Cuts at: 1108 4208 5003 6019 6041 1108 Size: 3100 795 1016 22 4044 Fragments arranged by size: 4044 3100 1016 795 22 PfoI T'CCnGG_A Cuts at: 3331 4581 5668 3331 Size: 1250 1087 6640 Fragments arranged by size: 6640 1250 1087 PmeI GTTT'AAAC Cuts at: 6281 6281 Size: 8977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6159 6191 7046 7078 8559 8591 6159 Size: 32 855 32 1481 32 6545 Fragments arranged by size: 6545 1481 855 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5895 5927 5959 6092 5895 Size: 32 32 133 8780 Fragments arranged by size: 8780 133 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8977 PsiI TTA'TAA Cuts at: 6547 6547 Size: 8977 PspOMI G'GGCC_C Cuts at: 4575 5139 5944 6031 6229 4575 Size: 564 805 87 198 7323 Fragments arranged by size: 7323 805 564 198 87 PstI C_TGCA'G Cuts at: 830 3073 6329 830 Size: 2243 3256 3478 Fragments arranged by size: 3478 3256 2243 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8977 PvuII CAG'CTG Cuts at: 2216 2356 3083 2216 Size: 140 727 8110 Fragments arranged by size: 8110 727 140 SacI G_AGCT'C Cuts at: 4239 4239 Size: 8977 SalI G'TCGA_C Cuts at: 1936 4906 6319 1936 Size: 2970 1413 4594 Fragments arranged by size: 4594 2970 1413 SanDI GG'GwC_CC Cuts at: 5895 6092 5895 Size: 197 8780 SapI GCTCTTCn'nnn_ Cuts at: 2472 5061 2472 Size: 2589 6388 SbfI CC_TGCA'GG Cuts at: 6329 6329 Size: 8977 ScaI AGT'ACT Cuts at: 2062 7225 8937 2062 Size: 5163 1712 2102 Fragments arranged by size: 5163 2102 1712 SfcI C'TryA_G Cuts at: 826 1045 2250 3069 5145 6325 7460 8141 8332 Size: 219 1205 819 2076 1180 1135 681 191 Cuts at: 8332 826 Size: 1471 Fragments arranged by size: 2076 1471 1205 1180 1135 819 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8977 SmaI CCC'GGG Cuts at: 1872 6304 1872 Size: 4432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2432 5577 7081 7982 8244 820 Size: 197 926 489 3145 1504 901 262 1553 Fragments arranged by size: 3145 1553 1504 926 901 489 262 197 SnaBI TAC'GTA Cuts at: 493 6768 493 Size: 6275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8977 SphI G_CATG'C Cuts at: 1132 6335 1132 Size: 5203 3774 SspI AAT'ATT Cuts at: 5 52 6901 5 Size: 47 6849 2081 Fragments arranged by size: 6849 2081 47 StuI AGG'CCT Cuts at: 1517 3131 3507 3719 5367 1517 Size: 1614 376 212 1648 5127 Fragments arranged by size: 5127 1648 1614 376 212 StyI C'CwwG_G Cuts at: 513 1065 2004 2943 3429 5154 5344 5487 5868 Size: 552 939 939 486 1725 190 143 381 Cuts at: 5868 6040 6087 6161 6416 513 Size: 172 47 74 255 3074 Fragments arranged by size: 3074 1725 939 939 552 486 381 255 190 172 143 74 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4105 4109 4198 4809 4869 4873 5042 Size: 1255 2525 4 89 611 60 4 169 Cuts at: 5042 5602 5966 7004 7021 7174 7359 7804 325 Size: 560 364 1038 17 153 185 445 1498 Fragments arranged by size: 2525 1498 1255 1038 611 560 445 364 185 169 153 89 60 17 4 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2060 2200 4190 Size: 274 80 33 51 1443 83 140 1990 Cuts at: 4190 7223 8935 96 Size: 3033 1712 138 Fragments arranged by size: 3033 1990 1712 1443 274 140 138 83 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5156 5188 8937 8969 5156 Size: 32 3749 32 5164 Fragments arranged by size: 5164 3749 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3484 1316 Size: 2168 6809 XbaI T'CTAG_A Cuts at: 6313 6313 Size: 8977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3865 4867 3865 Size: 1002 7975 XhoI C'TCGA_G Cuts at: 1943 5577 1943 Size: 3634 5343 XmaI C'CCGG_G Cuts at: 1870 6302 1870 Size: 4432 4545 XmnI GAAnn'nnTTC Cuts at: 2606 5621 6171 7106 2606 Size: 3015 550 935 4477 Fragments arranged by size: 4477 3015 935 550 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6785 276 Size: 53 83 186 936 5251 2468 Fragments arranged by size: 5251 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BbeI BbvCI BsiWI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI