Restriction map for
pFN21AA0460
(Linear) (Six-base) MAPSORT of: KIAA0460.seq Check: 9953 from: 1 to: 4272
KIAA0460.orf
With 182 enzymes: SgfI *
January 15, 2009 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 657 1383 1389 4272
Size: 657 726 6 2883
Fragments arranged by size:
2883 726 657 6
AccI GT'mk_AC
Cuts at: 0 284 2902 4272
Size: 284 2618 1370
Fragments arranged by size:
2618 1370 284
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 535 857 1501 1556 1685 1706 1826
Size: 224 311 322 644 55 129 21 120
Cuts at: 1826 1841 2619 4272
Size: 15 778 1653
Fragments arranged by size:
1653 778 644 322 311 224 129 120
55 21 15
AfeI AGC'GCT
Cuts at: 0 493 4272
Size: 493 3779
AgeI A'CCGG_T
Cuts at: 0 2666 4272
Size: 2666 1606
AlwNI CAG_nnn'CTG
Cuts at: 0 835 1147 1489 3847 4272
Size: 835 312 342 2358 425
Fragments arranged by size:
2358 835 425 342 312
ApaI G_GGCC'C
Cuts at: 0 2574 3138 3943 4030 4228 4272
Size: 2574 564 805 87 198 44
Fragments arranged by size:
2574 805 564 198 87 44
ApoI r'AATT_y
Cuts at: 0 448 613 643 713 2533 2911 3680 4272
Size: 448 165 30 70 1820 378 769 592
Fragments arranged by size:
1820 769 592 448 378 165 70 30
AvaI C'yCGr_G
Cuts at: 0 2225 3572 4272
Size: 2225 1347 700
Fragments arranged by size:
2225 1347 700
AvrII C'CTAG_G
Cuts at: 0 4156 4272
Size: 4156 116
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1076 1109 4272
Size: 1076 33 3163
Fragments arranged by size:
3163 1076 33
BamHI G'GATC_C
Cuts at: 0 225 4272
Size: 225 4047
BanI G'GyrC_C
Cuts at: 0 2629 3399 4272
Size: 2629 770 873
Fragments arranged by size:
2629 873 770
BanII G_rGCy'C
Cuts at: 0 1739 2234 2529 2574 3138 3943 4030 4228
Size: 1739 495 295 45 564 805 87 198
Cuts at: 4228 4272
Size: 44
Fragments arranged by size:
1739 805 564 495 295 198 87 45
44
BbsI GAAGACnn'nnnn_
Cuts at: 0 1001 4272
Size: 1001 3271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2019 2053 4272
Size: 2019 34 2219
Fragments arranged by size:
2219 2019 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1092 4272
Size: 1092 3180
BclI T'GATC_A
Cuts at: 0 3045 4272
Size: 3045 1227
BfrBI ATG'CAT
Cuts at: 0 760 4272
Size: 760 3512
BglI GCCn_nnn'nGGC
Cuts at: 0 1711 3368 3519 4272
Size: 1711 1657 151 753
Fragments arranged by size:
1711 1657 753 151
BglII A'GATC_T
Cuts at: 0 91 4272
Size: 91 4181
BlpI GC'TnA_GC
Cuts at: 0 3899 4272
Size: 3899 373
Bme1580I G_kGCm'C
Cuts at: 0 1635 2574 2989 3138 3402 3943 4030 4228
Size: 1635 939 415 149 264 541 87 198
Cuts at: 4228 4272
Size: 44
Fragments arranged by size:
1635 939 541 415 264 198 149 87
44
BmgBI CAC'GTC
Cuts at: 0 2858 2900 4272
Size: 2858 42 1372
Fragments arranged by size:
2858 1372 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 921 1135 1307 1531 1538 2753 2785 4272
Size: 921 214 172 224 7 1215 32 1487
Fragments arranged by size:
1487 1215 921 224 214 172 32 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 645 1896 4009 4272
Size: 645 1251 2113 263
Fragments arranged by size:
2113 1251 645 263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1611 1885 3686 3815 4272
Size: 1310 301 274 1801 129 457
Fragments arranged by size:
1801 1310 457 301 274 129
Bpu10I CC'TnA_GC
Cuts at: 0 1267 1963 3973 4272
Size: 1267 696 2010 299
Fragments arranged by size:
2010 1267 696 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 4272
Size: 412 3860
BsaI GGTCTCn'nnnn_
Cuts at: 0 1854 2307 4172 4194 4272
Size: 1854 453 1865 22 78
Fragments arranged by size:
1865 1854 453 78 22
BsaAI yAC'GTr
Cuts at: 0 2941 4272
Size: 2941 1331
BsaWI w'CCGG_w
Cuts at: 0 86 2666 4272
Size: 86 2580 1606
Fragments arranged by size:
2580 1606 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 859 889 1149 1179 1858 1888 3684 3714
Size: 859 30 260 30 679 30 1796 30
Cuts at: 3714 4272
Size: 558
Fragments arranged by size:
1796 859 679 558 260 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2774 3081 3084 3650 3692 3695 3761 4055
Size: 2774 307 3 566 42 3 66 294
Cuts at: 4055 4058 4272
Size: 3 214
Fragments arranged by size:
2774 566 307 294 214 66 42 3
3 3
BseYI C'CCAG_C
Cuts at: 0 1209 1345 2179 2977 3028 3810 4272
Size: 1209 136 834 798 51 782 462
Fragments arranged by size:
1209 834 798 782 462 136 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1361 1442 1475 4272
Size: 573 788 81 33 2797
Fragments arranged by size:
2797 788 573 81 33
BsiHKAI G_wGCw'C
Cuts at: 0 1570 2234 3068 3286 3674 3833 4272
Size: 1570 664 834 218 388 159 439
Fragments arranged by size:
1570 834 664 439 388 218 159
BsmI GAATG_Cn'
Cuts at: 0 1448 4272
Size: 1448 2824
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2608 3093 4272
Size: 2608 485 1179
Fragments arranged by size:
2608 1179 485
Bsp1286I G_dGCh'C
Cuts at: 0 1570 1635 1739 2234 2529 2574 2989 3068
Size: 1570 65 104 495 295 45 415 79
Cuts at: 3068 3138 3286 3402 3674 3833 3943 4030 4228
Size: 70 148 116 272 159 110 87 198
Cuts at: 4228 4272
Size: 44
Fragments arranged by size:
1570 495 415 295 272 198 159 148
116 110 104 87 79 70 65 45
44
BspEI T'CCGG_A
Cuts at: 0 86 4272
Size: 86 4186
BspHI T'CATG_A
Cuts at: 0 1286 4272
Size: 1286 2986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 657 1071 1383 1389 4272
Size: 657 414 312 6 2883
Fragments arranged by size:
2883 657 414 312 6
BsrBI CCG'CTC
Cuts at: 0 2836 4272
Size: 2836 1436
BsrFI r'CCGG_y
Cuts at: 0 2666 4272
Size: 2666 1606
BstBI TT'CG_AA
Cuts at: 0 3610 4272
Size: 3610 662
BstEII G'GTnAC_C
Cuts at: 0 2669 2845 4272
Size: 2669 176 1427
Fragments arranged by size:
2669 1427 176
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1773 2536 4272
Size: 1773 763 1736
Fragments arranged by size:
1773 1736 763
BstYI r'GATC_y
Cuts at: 0 91 225 831 1242 3603 4272
Size: 91 134 606 411 2361 669
Fragments arranged by size:
2361 669 606 411 134 91
BstZ17I GTA'TAC
Cuts at: 0 285 4272
Size: 285 3987
Bsu36I CC'TnA_GG
Cuts at: 0 3548 4272
Size: 3548 724
BtgI C'CryG_G
Cuts at: 0 938 2840 3933 4035 4272
Size: 938 1902 1093 102 237
Fragments arranged by size:
1902 1093 938 237 102
BtsI GCAGTG_nn'
Cuts at: 0 391 1492 2130 2280 3738 4077 4272
Size: 391 1101 638 150 1458 339 195
Fragments arranged by size:
1458 1101 638 391 339 195 150
DraI TTT'AAA
Cuts at: 0 261 794 1755 3375 4196 4272
Size: 261 533 961 1620 821 76
Fragments arranged by size:
1620 961 821 533 261 76
DraIII CAC_nnn'GTG
Cuts at: 0 3837 4272
Size: 3837 435
DrdI GACnn_nn'nnGTC
Cuts at: 0 2264 3207 4272
Size: 2264 943 1065
Fragments arranged by size:
2264 1065 943
EaeI y'GGCC_r
Cuts at: 0 2967 4141 4272
Size: 2967 1174 131
Fragments arranged by size:
2967 1174 131
EarI CTCTTCn'nnn_
Cuts at: 0 467 614 1134 1178 1698 2340 3056 4272
Size: 467 147 520 44 520 642 716 1216
Fragments arranged by size:
1216 716 642 520 520 467 147 44
EcoICRI GAG'CTC
Cuts at: 0 2232 4272
Size: 2232 2040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 535 857 1310 1501 1556 1611 1685
Size: 224 311 322 453 191 55 55 74
Cuts at: 1685 1706 1826 1841 1885 2619 3686 3815 4272
Size: 21 120 15 44 734 1067 129 457
Fragments arranged by size:
1067 734 457 453 322 311 224 191
129 120 74 55 55 44 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1325 1423 4272
Size: 1325 98 2849
Fragments arranged by size:
2849 1325 98
EcoO109I rG'GnC_Cy
Cuts at: 0 462 2407 2570 2571 3134 3135 3890 3922
Size: 462 1945 163 1 563 1 755 32
Cuts at: 3922 3939 3940 3954 4087 4224 4225 4272
Size: 17 1 14 133 137 1 47
Fragments arranged by size:
1945 755 563 462 163 137 133 47
32 17 14 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 3680 4272
Size: 3680 592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1766 2568 4272
Size: 1766 802 1704
Fragments arranged by size:
1766 1704 802
HaeII r_GCGC'y
Cuts at: 0 495 1985 4272
Size: 495 1490 2287
Fragments arranged by size:
2287 1490 495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 859 891 966 998 1012 1044 1149 1181
Size: 859 32 75 32 14 32 105 32
Cuts at: 1181 1380 1412 1858 1890 2122 2154 2302 2334
Size: 199 32 446 32 232 32 148 32
Cuts at: 2334 2590 2622 3319 3351 4091 4123 4272
Size: 256 32 697 32 740 32 149
Fragments arranged by size:
859 740 697 446 256 232 199 149
148 105 75 32 32 32 32 32
32 32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 0 533 821 2903 3786 4272
Size: 533 288 2082 883 486
Fragments arranged by size:
2082 883 533 486 288
HindIII A'AGCT_T
Cuts at: 0 317 1653 4272
Size: 317 1336 2619
Fragments arranged by size:
2619 1336 317
MfeI C'AATT_G
Cuts at: 0 811 1718 4272
Size: 811 907 2554
Fragments arranged by size:
2554 907 811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1790 2738 3461 4272
Size: 785 1005 948 723 811
Fragments arranged by size:
1005 948 811 785 723
MscI TGG'CCA
Cuts at: 0 2969 4272
Size: 2969 1303
MslI CAynn'nnrTG
Cuts at: 0 3248 4272
Size: 3248 1024
MspA1I CmG'CkG
Cuts at: 0 211 351 1078 3356 4272
Size: 211 140 727 2278 916
Fragments arranged by size:
2278 916 727 211 140
NcoI C'CATG_G
Cuts at: 0 938 4035 4272
Size: 938 3097 237
Fragments arranged by size:
3097 938 237
NsiI A_TGCA'T
Cuts at: 0 762 4272
Size: 762 3510
PasI CC'CwG_GG
Cuts at: 0 1732 2203 2617 3926 4272
Size: 1732 471 414 1309 346
Fragments arranged by size:
1732 1309 471 414 346
PflMI CCAn_nnn'nTGG
Cuts at: 0 2203 2998 4014 4036 4272
Size: 2203 795 1016 22 236
Fragments arranged by size:
2203 1016 795 236 22
PfoI T'CCnGG_A
Cuts at: 0 1326 2576 3663 4272
Size: 1326 1250 1087 609
Fragments arranged by size:
1326 1250 1087 609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4154 4186 4272
Size: 4154 32 86
Fragments arranged by size:
4154 86 32
PpuMI rG'GwC_Cy
Cuts at: 0 3890 3922 3954 4087 4272
Size: 3890 32 32 133 185
Fragments arranged by size:
3890 185 133 32 32
PspOMI G'GGCC_C
Cuts at: 0 2570 3134 3939 4026 4224 4272
Size: 2570 564 805 87 198 48
Fragments arranged by size:
2570 805 564 198 87 48
PstI C_TGCA'G
Cuts at: 0 1068 4272
Size: 1068 3204
PvuII CAG'CTG
Cuts at: 0 211 351 1078 4272
Size: 211 140 727 3194
Fragments arranged by size:
3194 727 211 140
SacI G_AGCT'C
Cuts at: 0 2234 4272
Size: 2234 2038
SalI G'TCGA_C
Cuts at: 0 2901 4272
Size: 2901 1371
SanDI GG'GwC_CC
Cuts at: 0 3890 4087 4272
Size: 3890 197 185
Fragments arranged by size:
3890 197 185
SapI GCTCTTCn'nnn_
Cuts at: 0 467 3056 4272
Size: 467 2589 1216
Fragments arranged by size:
2589 1216 467
ScaI AGT'ACT
Cuts at: 0 57 4272
Size: 57 4215
SfcI C'TryA_G
Cuts at: 0 245 1064 3140 4272
Size: 245 819 2076 1132
Fragments arranged by size:
2076 1132 819 245
SmlI C'TyrA_G
Cuts at: 0 427 3572 4272
Size: 427 3145 700
Fragments arranged by size:
3145 700 427
StuI AGG'CCT
Cuts at: 0 1126 1502 1714 3362 4272
Size: 1126 376 212 1648 910
Fragments arranged by size:
1648 1126 910 376 212
StyI C'CwwG_G
Cuts at: 0 938 1424 3149 3339 3482 3863 4035 4082
Size: 938 486 1725 190 143 381 172 47
Cuts at: 4082 4156 4272
Size: 74 116
Fragments arranged by size:
1725 938 486 381 190 172 143 116
74 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2100 2104 2193 2804 2864 2868 3037 3597
Size: 2100 4 89 611 60 4 169 560
Cuts at: 3597 3961 4272
Size: 364 311
Fragments arranged by size:
2100 611 560 364 311 169 89 60
4 4
TatI w'GTAC_w
Cuts at: 0 55 195 2185 4272
Size: 55 140 1990 2087
Fragments arranged by size:
2087 1990 140 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3151 3183 4272
Size: 3151 32 1089
Fragments arranged by size:
3151 1089 32
Tth111I GACn'n_nGTC
Cuts at: 0 1479 4272
Size: 1479 2793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1860 2862 4272
Size: 1860 1002 1410
Fragments arranged by size:
1860 1410 1002
XhoI C'TCGA_G
Cuts at: 0 3572 4272
Size: 3572 700
XmnI GAAnn'nnTTC
Cuts at: 0 601 3616 4166 4272
Size: 601 3015 550 106
Fragments arranged by size:
3015 601 550 106
Enzymes that do cut:
AarI AccI AcuI AfeI AgeI AlwNI ApaI ApoI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI DraI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NsiI PasI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII SacI SalI SanDI SapI ScaI
SfcI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI BbeI BbvCI BmtI
BsaBI BsaHI BsiEI BsiWI BsrDI BsrGI BssHII BssSI
BstAPI BtgZI ClaI CspCI EagI EciI EcoRV FseI
FspI FspAI HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XmaI
ZraI