(Circular) (Six-base) MAPSORT of: pFN21AE0173.seq Check: 9733 from: 1 to: 5599 pFN21AE0173 5599 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2431 2431 Size: 5599 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2052 3409 278 Size: 53 83 186 936 516 1357 2468 Fragments arranged by size: 2468 1357 936 516 186 83 53 AccI GT'mk_AC Cuts at: 1937 2942 1937 Size: 1005 4594 Acc65I G'GTAC_C Cuts at: 2920 2920 Size: 5599 AclI AA'CG_TT Cuts at: 1563 3726 4099 1563 Size: 2163 373 3063 Fragments arranged by size: 3063 2163 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2785 3662 5046 5314 1632 Size: 1153 877 1384 268 1917 Fragments arranged by size: 1917 1384 1153 877 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5402 AflIII A'CryG_T Cuts at: 1203 2130 4498 5475 1203 Size: 927 2368 977 1327 Fragments arranged by size: 2368 1327 977 927 AgeI A'CCGG_T Cuts at: 4430 4430 Size: 5599 AhdI GACnn_n'nnGTC Cuts at: 4328 4328 Size: 5599 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5599 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5599 AlwNI CAG_nnn'CTG Cuts at: 2023 3342 4914 2023 Size: 1319 1572 2708 Fragments arranged by size: 2708 1572 1319 ApaI G_GGCC'C Cuts at: 2708 2708 Size: 5599 ApaLI G'TGCA_C Cuts at: 3656 4812 3656 Size: 1156 4443 ApoI r'AATT_y Cuts at: 1493 2908 3134 3286 5395 1493 Size: 1415 226 152 2109 1697 Fragments arranged by size: 2109 1697 1415 226 152 AseI AT'TA_AT Cuts at: 160 1032 4153 160 Size: 872 3121 1606 Fragments arranged by size: 3121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 AvaI C'yCGr_G Cuts at: 1870 1943 2924 1870 Size: 73 981 4545 Fragments arranged by size: 4545 981 73 BamHI G'GATC_C Cuts at: 2409 2929 3296 2409 Size: 520 367 4712 Fragments arranged by size: 4712 520 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2690 2847 2920 4375 618 Size: 325 217 627 903 157 73 1455 1842 Fragments arranged by size: 1842 1455 903 627 325 217 157 73 BanII G_rGCy'C Cuts at: 1391 2170 2189 2541 2708 2918 1391 Size: 779 19 352 167 210 4072 Fragments arranged by size: 4072 779 352 210 167 19 BbeI G_GCGC'C Cuts at: 2694 2694 Size: 5599 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4628 BbvCI CC'TCA_GC Cuts at: 2682 2682 Size: 5599 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3790 3824 1903 Size: 34 1853 34 3678 Fragments arranged by size: 3678 1853 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2752 3491 4701 2752 Size: 739 1210 3650 Fragments arranged by size: 3650 1210 739 BclI T'GATC_A Cuts at: 1548 1548 Size: 5599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2712 2880 4210 Size: 107 122 71 631 605 1040 168 1330 Cuts at: 4210 136 Size: 1525 Fragments arranged by size: 1525 1330 1040 631 605 168 122 107 71 BglII A'GATC_T Cuts at: 5594 5594 Size: 5599 BlpI GC'TnA_GC Cuts at: 3016 3016 Size: 5599 Bme1580I G_kGCm'C Cuts at: 1790 2708 3660 4816 1790 Size: 918 952 1156 2573 Fragments arranged by size: 2573 1156 952 918 BmgBI CAC'GTC Cuts at: 1318 2566 1318 Size: 1248 4351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2392 2774 3330 Size: 416 53 83 399 24 975 382 556 Cuts at: 3330 4288 442 Size: 958 1753 Fragments arranged by size: 1753 975 958 556 416 399 382 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5599 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2555 2555 Size: 5599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2472 2793 4259 1239 Size: 1233 321 1466 2579 Fragments arranged by size: 2579 1466 1233 321 Bpu10I CC'TnA_GC Cuts at: 2682 2682 Size: 5599 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2647 3724 4589 4887 2647 Size: 1077 865 298 3359 Fragments arranged by size: 3359 1077 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4262 882 Size: 617 2763 2219 Fragments arranged by size: 2763 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3390 493 Size: 711 2186 2702 Fragments arranged by size: 2702 2186 711 BsaBI GATnn'nnATC Cuts at: 5593 5593 Size: 5599 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2691 2697 3406 Size: 53 83 186 936 516 642 6 709 Cuts at: 3406 3788 4423 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 709 642 635 516 382 186 83 53 6 BsaWI w'CCGG_w Cuts at: 4032 4430 4704 4851 5262 4032 Size: 398 274 147 411 4369 Fragments arranged by size: 4369 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 2553 2583 1178 Size: 30 165 30 678 30 442 30 4194 Fragments arranged by size: 4194 678 442 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2549 2699 2833 1186 Size: 1363 150 134 3952 Fragments arranged by size: 3952 1363 150 134 BseYI C'CCAG_C Cuts at: 4802 5298 4802 Size: 496 5103 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2128 2846 1163 Size: 573 392 718 3916 Fragments arranged by size: 3916 718 573 392 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3049 3810 4838 1540 Size: 275 186 1048 761 1028 2301 Fragments arranged by size: 2301 1048 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2541 3660 3745 4816 2541 Size: 1119 85 1071 3324 Fragments arranged by size: 3324 1119 1071 85 BsmI GAATG_Cn' Cuts at: 3110 3203 3110 Size: 93 5506 BsmBI CGTCTCn'nnnn_ Cuts at: 2556 2556 Size: 5599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2170 2189 2541 2708 2918 3660 3745 Size: 399 380 19 352 167 210 742 85 Cuts at: 3745 4816 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 352 210 167 85 19 BspEI T'CCGG_A Cuts at: 5262 5262 Size: 5599 BspHI T'CATG_A Cuts at: 3488 5218 3488 Size: 1730 3869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2431 2954 844 Size: 1587 523 3489 Fragments arranged by size: 3489 1587 523 BsrBI CCG'CTC Cuts at: 1394 1823 3486 1394 Size: 429 1663 3507 Fragments arranged by size: 3507 1663 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4094 4268 65 Size: 1183 2846 174 1396 Fragments arranged by size: 2846 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2858 4243 4430 1700 Size: 110 1048 1385 187 2869 Fragments arranged by size: 2869 1385 1048 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5599 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5599 BssSI C'ACGA_G Cuts at: 2529 3659 4671 2529 Size: 1130 1012 3457 Fragments arranged by size: 3457 1130 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5599 BstBI TT'CG_AA Cuts at: 4403 4403 Size: 5599 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5599 BstXI CCAn_nnnn'nTGG Cuts at: 2552 2599 2552 Size: 47 5552 BstYI r'GATC_y Cuts at: 1972 2019 2409 2499 2929 3296 3681 3698 5150 Size: 47 390 90 430 367 385 17 1452 Cuts at: 5150 5236 5248 5594 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 430 390 385 367 346 90 86 47 17 12 Bsu36I CC'TnA_GG Cuts at: 2076 2076 Size: 5599 BtgI C'CryG_G Cuts at: 513 1065 2004 2295 2511 513 Size: 552 939 291 216 3601 Fragments arranged by size: 3601 939 552 291 216 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2275 487 Size: 683 56 1049 3811 Fragments arranged by size: 3811 1049 683 56 BtsI GCAGTG_nn' Cuts at: 3115 3909 3929 3115 Size: 794 20 4785 Fragments arranged by size: 4785 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2851 2886 529 Size: 35 2287 35 3242 Fragments arranged by size: 3242 2287 35 35 DraI TTT'AAA Cuts at: 2903 2903 Size: 5599 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4606 809 Size: 507 3290 1802 Fragments arranged by size: 3290 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2200 2589 2856 Size: 54 1425 50 275 18 370 389 267 Cuts at: 2856 3046 3935 5564 8 Size: 190 889 1629 43 Fragments arranged by size: 1629 1425 889 389 370 275 267 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3046 1537 Size: 275 1234 4090 Fragments arranged by size: 4090 1234 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2004 2546 3529 1353 Size: 651 542 983 3423 Fragments arranged by size: 3423 983 651 542 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2222 4186 4560 4706 387 Size: 1064 138 633 1964 374 146 1280 Fragments arranged by size: 1964 1280 1064 633 374 146 138 EcoICRI GAG'CTC Cuts at: 2539 2539 Size: 5599 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2472 2785 2793 3662 4259 5046 5314 Size: 393 840 313 8 869 597 787 268 Cuts at: 5314 1239 Size: 1524 Fragments arranged by size: 1524 869 840 787 597 393 313 268 8 EcoNI CCTnn'n_nnAGG Cuts at: 2810 2810 Size: 5599 EcoO109I rG'GnC_Cy Cuts at: 2705 2705 Size: 5599 EcoRI G'AATT_C Cuts at: 2908 3286 2908 Size: 378 5221 FspI TGC'GCA Cuts at: 4105 4105 Size: 5599 HaeII r_GCGC'y Cuts at: 1678 2694 4746 1678 Size: 1016 2052 2531 Fragments arranged by size: 2531 2052 1016 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4240 4272 4314 4346 1373 Size: 32 135 32 2668 32 42 32 2626 Fragments arranged by size: 2668 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2943 3189 669 Size: 988 281 1005 246 3079 Fragments arranged by size: 3079 1005 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5599 HpaI GTT'AAC Cuts at: 3189 3189 Size: 5599 KasI G'GCGC_C Cuts at: 2690 2690 Size: 5599 KpnI G_GTAC'C Cuts at: 2924 2924 Size: 5599 MfeI C'AATT_G Cuts at: 3198 3198 Size: 5599 MluI A'CGCG_T Cuts at: 2130 5475 2130 Size: 3345 2254 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2160 4713 4897 1305 Size: 855 2553 184 2007 Fragments arranged by size: 2553 2007 855 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2202 2591 10 Size: 54 1425 343 370 389 3018 Fragments arranged by size: 3018 1425 389 370 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2730 3557 3916 4075 518 Size: 648 1564 827 359 159 2042 Fragments arranged by size: 2042 1564 827 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2458 2548 3015 3692 4840 5085 1606 Size: 852 90 467 677 1148 245 2120 Fragments arranged by size: 2120 1148 852 677 467 245 90 NaeI GCC'GGC Cuts at: 2860 2860 Size: 5599 NarI GG'CG_CC Cuts at: 2691 2691 Size: 5599 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4108 Fragments arranged by size: 4108 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5599 NgoMIV G'CCGG_C Cuts at: 2858 2858 Size: 5599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5599 NotI GC'GGCC_GC Cuts at: 3046 3046 Size: 5599 NspI r_CATG'y Cuts at: 1132 1229 2957 4502 1132 Size: 97 1728 1545 2229 Fragments arranged by size: 2229 1728 1545 97 PasI CC'CwG_GG Cuts at: 1348 2701 2812 1348 Size: 1353 111 4135 Fragments arranged by size: 4135 1353 111 PciI A'CATG_T Cuts at: 4498 4498 Size: 5599 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5599 PfoI T'CCnGG_A Cuts at: 2220 2220 Size: 5599 PmeI GTTT'AAAC Cuts at: 2903 2903 Size: 5599 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3668 3700 5181 5213 3668 Size: 32 1481 32 4054 Fragments arranged by size: 4054 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5599 PsiI TTA'TAA Cuts at: 3169 3169 Size: 5599 PspOMI G'GGCC_C Cuts at: 2704 2704 Size: 5599 PstI C_TGCA'G Cuts at: 830 2457 2880 2951 830 Size: 1627 423 71 3478 Fragments arranged by size: 3478 1627 423 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5599 PvuII CAG'CTG Cuts at: 2548 2548 Size: 5599 SacI G_AGCT'C Cuts at: 2541 2541 Size: 5599 SalI G'TCGA_C Cuts at: 1936 2941 1936 Size: 1005 4594 SapI GCTCTTCn'nnn_ Cuts at: 2546 2546 Size: 5599 SbfI CC_TGCA'GG Cuts at: 2880 2951 2880 Size: 71 5528 ScaI AGT'ACT Cuts at: 3847 5559 3847 Size: 1712 3887 SfcI C'TryA_G Cuts at: 826 1045 2370 2453 2876 2947 4082 4763 4954 Size: 219 1325 83 423 71 1135 681 191 Cuts at: 4954 826 Size: 1471 Fragments arranged by size: 1471 1325 1135 681 423 219 191 83 71 SfoI GGC'GCC Cuts at: 2692 2692 Size: 5599 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5599 SmaI CCC'GGG Cuts at: 1872 2926 1872 Size: 1054 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2626 3703 4604 4866 820 Size: 197 926 683 1077 901 262 1553 Fragments arranged by size: 1553 1077 926 901 683 262 197 SnaBI TAC'GTA Cuts at: 493 3390 493 Size: 2897 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5599 SphI G_CATG'C Cuts at: 1132 2957 1132 Size: 1825 3774 SspI AAT'ATT Cuts at: 5 52 3523 5 Size: 47 3471 2081 Fragments arranged by size: 3471 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5599 StyI C'CwwG_G Cuts at: 513 1065 2004 3038 513 Size: 552 939 1034 3074 Fragments arranged by size: 3074 1034 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3626 3643 3796 3981 4426 325 Size: 1255 2046 17 153 185 445 1498 Fragments arranged by size: 2046 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3845 5557 96 Size: 274 80 33 51 1443 1868 1712 138 Fragments arranged by size: 1868 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2081 2113 2553 2585 5559 5591 2081 Size: 32 440 32 2974 32 2089 Fragments arranged by size: 2974 2089 440 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5599 XbaI T'CTAG_A Cuts at: 2935 2935 Size: 5599 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5599 XmaI C'CCGG_G Cuts at: 1870 2924 1870 Size: 1054 4545 XmnI GAAnn'nnTTC Cuts at: 3728 3728 Size: 5599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2050 3407 276 Size: 53 83 186 936 516 1357 2468 Fragments arranged by size: 2468 1357 936 516 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI