(Circular) (Six-base) MAPSORT of: pFN21AA1082.seq Check: 9695 from: 1 to: 9988 pFN21AA1082 9988 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3148 7198 3148 Size: 4050 5938 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7798 278 Size: 53 83 186 936 6262 2468 Fragments arranged by size: 6262 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2931 3035 5389 6333 7331 1937 Size: 994 104 2354 944 998 4594 Fragments arranged by size: 4594 2354 998 994 944 104 Acc65I G'GTAC_C Cuts at: 4767 6960 7309 4767 Size: 2193 349 7446 Fragments arranged by size: 7446 2193 349 AclI AA'CG_TT Cuts at: 1563 8115 8488 1563 Size: 6552 373 3063 Fragments arranged by size: 6552 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4526 4697 5589 8051 9435 9703 1632 Size: 2894 171 892 2462 1384 268 1917 Fragments arranged by size: 2894 2462 1917 1384 892 268 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9791 AflIII A'CryG_T Cuts at: 1203 7129 8887 9864 1203 Size: 5926 1758 977 1327 Fragments arranged by size: 5926 1758 1327 977 AgeI A'CCGG_T Cuts at: 8819 8819 Size: 9988 AhdI GACnn_n'nnGTC Cuts at: 5417 8717 5417 Size: 3300 6688 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9988 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2057 6870 1184 Size: 873 4813 4302 Fragments arranged by size: 4813 4302 873 AlwNI CAG_nnn'CTG Cuts at: 3320 3338 6354 7731 9303 3320 Size: 18 3016 1377 1572 4005 Fragments arranged by size: 4005 3016 1572 1377 18 ApaI G_GGCC'C Cuts at: 2973 4148 4544 6617 2973 Size: 1175 396 2073 6344 Fragments arranged by size: 6344 2073 1175 396 ApaLI G'TGCA_C Cuts at: 8045 9201 8045 Size: 1156 8832 ApoI r'AATT_y Cuts at: 1493 2207 3385 3394 4512 6314 7160 7297 7523 Size: 714 1178 9 1118 1802 846 137 226 Cuts at: 7523 7675 9784 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1802 1697 1178 1118 846 714 226 152 137 9 AseI AT'TA_AT Cuts at: 160 1032 8542 160 Size: 872 7510 1606 Fragments arranged by size: 7510 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9988 AvaI C'yCGr_G Cuts at: 1870 1943 2136 3376 3891 4028 5346 7313 1870 Size: 73 193 1240 515 137 1318 1967 4545 Fragments arranged by size: 4545 1967 1318 1240 515 193 137 73 AvrII C'CTAG_G Cuts at: 7033 7033 Size: 9988 BamHI G'GATC_C Cuts at: 7318 7685 7318 Size: 367 9621 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 2299 4015 4726 4767 Size: 325 217 627 392 120 1716 711 41 Cuts at: 4767 5506 6960 7250 7309 8764 618 Size: 739 1454 290 59 1455 1842 Fragments arranged by size: 1842 1716 1455 1454 739 711 627 392 325 290 217 120 59 41 BanII G_rGCy'C Cuts at: 1391 2973 3701 4127 4148 4544 4695 6288 6617 Size: 1582 728 426 21 396 151 1593 329 Cuts at: 6617 7066 7307 1391 Size: 449 241 4072 Fragments arranged by size: 4072 1593 1582 728 449 426 396 329 241 151 21 BbeI G_GCGC'C Cuts at: 2303 2303 Size: 9988 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5950 7116 928 Size: 971 4051 1166 3800 Fragments arranged by size: 4051 3800 1166 971 BbvCI CC'TCA_GC Cuts at: 2071 2256 5971 2071 Size: 185 3715 6088 Fragments arranged by size: 6088 3715 185 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4727 4761 6767 6801 8179 8213 1903 Size: 34 2790 34 2006 34 1378 34 3678 Fragments arranged by size: 3678 2790 2006 1378 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7880 9090 7880 Size: 1210 8778 BclI T'GATC_A Cuts at: 1548 2547 6931 1548 Size: 999 4384 4605 Fragments arranged by size: 4605 4384 999 BfrBI ATG'CAT Cuts at: 3591 4716 3591 Size: 1125 8863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 8599 136 Size: 107 122 71 631 605 475 6452 1525 Fragments arranged by size: 6452 1525 631 605 475 122 107 71 BglII A'GATC_T Cuts at: 4951 5564 6213 6514 9983 4951 Size: 613 649 301 3469 4956 Fragments arranged by size: 4956 3469 649 613 301 BlpI GC'TnA_GC Cuts at: 4126 4563 7405 4126 Size: 437 2842 6709 Fragments arranged by size: 6709 2842 437 Bme1580I G_kGCm'C Cuts at: 1790 2184 2973 4148 4544 6617 8049 9205 1790 Size: 394 789 1175 396 2073 1432 1156 2573 Fragments arranged by size: 2573 2073 1432 1175 1156 789 396 394 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9988 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2309 2399 5140 Size: 416 53 83 399 24 892 90 2741 Cuts at: 5140 7719 8677 442 Size: 2579 958 1753 Fragments arranged by size: 2741 2579 1753 958 892 416 399 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9988 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 4054 3446 Size: 608 9380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2309 3795 4162 4423 4707 4781 5505 7080 Size: 1070 1486 367 261 284 74 724 1575 Cuts at: 7080 7107 8648 1239 Size: 27 1541 2579 Fragments arranged by size: 2579 1575 1541 1486 1070 724 367 284 261 74 27 Bpu10I CC'TnA_GC Cuts at: 2071 2256 3695 3700 5180 5971 2071 Size: 185 1439 5 1480 791 6088 Fragments arranged by size: 6088 1480 1439 791 185 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3707 6260 6443 6749 7241 8113 8978 9276 3707 Size: 2553 183 306 492 872 865 298 4419 Fragments arranged by size: 4419 2553 872 865 492 306 298 183 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2292 4952 5404 5806 5880 6315 8651 Size: 617 793 2660 452 402 74 435 2336 Cuts at: 8651 882 Size: 2219 Fragments arranged by size: 2660 2336 2219 793 617 452 435 402 74 BsaAI yAC'GTr Cuts at: 493 1204 7779 493 Size: 711 6575 2702 Fragments arranged by size: 6575 2702 711 BsaBI GATnn'nnATC Cuts at: 6731 9982 6731 Size: 3251 6737 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2300 7795 8177 8812 Size: 53 83 186 936 767 5495 382 635 Cuts at: 8812 275 Size: 1451 Fragments arranged by size: 5495 1451 936 767 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3774 4770 4830 8421 8819 9093 9240 9651 3774 Size: 996 60 3591 398 274 147 411 4111 Fragments arranged by size: 4111 3591 996 411 398 274 147 60 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2356 2386 2854 2884 3793 Size: 30 165 30 953 30 468 30 909 Cuts at: 3793 3813 3823 3843 4421 4451 5319 5349 1178 Size: 20 10 20 578 30 868 30 5817 Fragments arranged by size: 5817 953 909 868 578 468 165 30 30 30 30 30 30 20 20 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2875 3973 4030 4087 4219 4259 4277 4493 Size: 1689 1098 57 57 132 40 18 216 Cuts at: 4493 5511 5650 7010 1186 Size: 1018 139 1360 4164 Fragments arranged by size: 4164 1689 1360 1098 1018 216 139 132 57 57 40 18 BseYI C'CCAG_C Cuts at: 4019 4537 4573 4887 5839 6191 7014 9191 9687 Size: 518 36 314 952 352 823 2177 496 Cuts at: 9687 4019 Size: 4320 Fragments arranged by size: 4320 2177 952 823 518 496 352 314 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2505 3131 3522 4721 5654 7046 1163 Size: 573 769 626 391 1199 933 1392 4105 Fragments arranged by size: 4105 1392 1199 933 769 626 573 391 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3368 7438 8199 9227 1540 Size: 275 186 1367 4070 761 1028 2301 Fragments arranged by size: 4070 2301 1367 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3427 3701 4524 5745 6288 6453 6748 8049 8134 Size: 274 823 1221 543 165 295 1301 85 Cuts at: 8134 9205 3427 Size: 1071 4210 Fragments arranged by size: 4210 1301 1221 1071 823 543 295 274 165 85 BsmI GAATG_Cn' Cuts at: 2331 2992 4757 7499 7592 2331 Size: 661 1765 2742 93 4727 Fragments arranged by size: 4727 2742 1765 661 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2973 3427 3701 4127 4148 4524 Size: 399 394 789 454 274 426 21 376 Cuts at: 4524 4544 4695 5745 6288 6453 6617 6748 7066 Size: 20 151 1050 543 165 164 131 318 Cuts at: 7066 7307 8049 8134 9205 1391 Size: 241 742 85 1071 2174 Fragments arranged by size: 2174 1071 1050 789 742 543 454 426 399 394 376 318 274 241 165 164 151 131 85 21 20 BspEI T'CCGG_A Cuts at: 4830 9651 4830 Size: 4821 5167 BspHI T'CATG_A Cuts at: 3856 6748 6814 6934 6946 7192 7264 7877 9607 Size: 2892 66 120 12 246 72 613 1730 Cuts at: 9607 3856 Size: 4237 Fragments arranged by size: 4237 2892 1730 613 246 120 72 66 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3148 5422 7063 7198 7343 844 Size: 2304 2274 1641 135 145 3489 Fragments arranged by size: 3489 2304 2274 1641 145 135 BsrBI CCG'CTC Cuts at: 1394 1823 2109 2172 4176 4765 7875 1394 Size: 429 286 63 2004 589 3110 3507 Fragments arranged by size: 3507 3110 2004 589 429 286 63 BsrDI GCAATG_nn' Cuts at: 65 1248 2343 6021 8483 8657 65 Size: 1183 1095 3678 2462 174 1396 Fragments arranged by size: 3678 2462 1396 1183 1095 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2150 3363 4608 5175 5190 5503 8632 Size: 110 340 1213 1245 567 15 313 3129 Cuts at: 8632 8819 1700 Size: 187 2869 Fragments arranged by size: 3129 2869 1245 1213 567 340 313 187 110 15 BsrGI T'GTAC_A Cuts at: 96 6624 96 Size: 6528 3460 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9988 BssSI C'ACGA_G Cuts at: 8048 9060 8048 Size: 1012 8976 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9988 BstBI TT'CG_AA Cuts at: 8792 8792 Size: 9988 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9988 BstXI CCAn_nnnn'nTGG Cuts at: 3482 5035 6317 3482 Size: 1553 1282 7153 Fragments arranged by size: 7153 1553 1282 BstYI r'GATC_y Cuts at: 1972 2113 2731 3309 3719 4951 5564 6133 6213 Size: 141 618 578 410 1232 613 569 80 Cuts at: 6213 6514 6876 7318 7685 8070 8087 9539 9625 Size: 301 362 442 367 385 17 1452 86 Cuts at: 9625 9637 9983 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1232 618 613 578 569 442 410 385 367 362 346 301 141 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 5732 5732 Size: 9988 BtgI C'CryG_G Cuts at: 513 1065 2004 2091 3821 4161 513 Size: 552 939 87 1730 340 6340 Fragments arranged by size: 6340 1730 939 552 340 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2961 3426 487 Size: 683 56 1735 465 7049 Fragments arranged by size: 7049 1735 683 465 56 BtsI GCAGTG_nn' Cuts at: 2061 4564 5206 5790 5847 7239 7504 8298 8318 Size: 2503 642 584 57 1392 265 794 20 Cuts at: 8318 2061 Size: 3731 Fragments arranged by size: 3731 2503 1392 794 642 584 265 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9988 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5117 5152 529 Size: 35 4553 35 5365 Fragments arranged by size: 5365 4553 35 35 DraI TTT'AAA Cuts at: 7292 7292 Size: 9988 DraIII CAC_nnn'GTG Cuts at: 5201 5201 Size: 9988 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8995 809 Size: 507 7679 1802 Fragments arranged by size: 7679 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2354 2854 3244 Size: 54 1425 50 275 18 524 500 390 Cuts at: 3244 4171 4636 4977 5192 7435 8324 9953 8 Size: 927 465 341 215 2243 889 1629 43 Fragments arranged by size: 2243 1629 1425 927 889 524 500 465 390 341 275 215 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7435 1537 Size: 275 5623 4090 Fragments arranged by size: 5623 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2822 3415 3891 3991 4085 4933 5123 5205 Size: 1469 593 476 100 94 848 190 82 Cuts at: 5205 5470 5565 5978 6655 6750 7918 1353 Size: 265 95 413 677 95 1168 3423 Fragments arranged by size: 3423 1469 1168 848 677 593 476 413 265 190 100 95 95 94 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5508 5653 7136 8575 8949 Size: 1064 138 435 3484 145 1483 1439 374 Cuts at: 8949 9095 387 Size: 146 1280 Fragments arranged by size: 3484 1483 1439 1280 1064 435 374 146 145 138 EcoICRI GAG'CTC Cuts at: 3699 6286 3699 Size: 2587 7401 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2309 3795 4162 4423 4526 4697 4707 Size: 393 677 1486 367 261 103 171 10 Cuts at: 4707 4781 5505 5589 7080 7107 8051 8648 9435 Size: 74 724 84 1491 27 944 597 787 Cuts at: 9435 9703 1239 Size: 268 1524 Fragments arranged by size: 1524 1491 1486 944 787 724 677 597 393 367 268 261 171 103 84 74 27 10 EcoNI CCTnn'n_nnAGG Cuts at: 2324 7154 2324 Size: 4830 5158 EcoO109I rG'GnC_Cy Cuts at: 2120 2188 2311 2585 2969 2970 4144 4262 4541 Size: 68 123 274 384 1 1174 118 279 Cuts at: 4541 4597 5727 5843 6614 2120 Size: 56 1130 116 771 5494 Fragments arranged by size: 5494 1174 1130 771 384 279 274 123 118 116 68 56 1 EcoRI G'AATT_C Cuts at: 3385 4512 7160 7297 7675 3385 Size: 1127 2648 137 378 5698 Fragments arranged by size: 5698 2648 1127 378 137 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2306 4434 2306 Size: 2128 7860 FseI GG_CCGG'CC Cuts at: 4612 4612 Size: 9988 FspI TGC'GCA Cuts at: 2132 8494 2132 Size: 6362 3626 HaeII r_GCGC'y Cuts at: 1678 2303 9135 1678 Size: 625 6832 2531 Fragments arranged by size: 6832 2531 625 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2098 2130 2356 2388 2882 Size: 32 135 32 526 32 226 32 494 Cuts at: 2882 2914 4040 4072 4207 4239 4421 4453 4831 Size: 32 1126 32 135 32 182 32 378 Cuts at: 4831 4863 5142 5174 5403 5435 5549 5581 7103 Size: 32 279 32 229 32 114 32 1522 Cuts at: 7103 7135 8629 8661 8703 8735 1373 Size: 32 1494 32 42 32 2626 Fragments arranged by size: 2626 1522 1494 1126 526 494 378 279 229 226 182 135 135 114 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5390 7332 7578 669 Size: 988 281 3452 1942 246 3079 Fragments arranged by size: 3452 3079 1942 988 281 246 HindIII A'AGCT_T Cuts at: 748 4411 5819 748 Size: 3663 1408 4917 Fragments arranged by size: 4917 3663 1408 HpaI GTT'AAC Cuts at: 7578 7578 Size: 9988 KasI G'GCGC_C Cuts at: 2299 2299 Size: 9988 KpnI G_GTAC'C Cuts at: 4771 6964 7313 4771 Size: 2193 349 7446 Fragments arranged by size: 7446 2193 349 MfeI C'AATT_G Cuts at: 6205 6771 7587 6205 Size: 566 816 8606 Fragments arranged by size: 8606 816 566 MluI A'CGCG_T Cuts at: 9864 9864 Size: 9988 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5880 6209 6880 9102 9286 1305 Size: 4575 329 671 2222 184 2007 Fragments arranged by size: 4575 2222 2007 671 329 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2356 3246 4638 4979 10 Size: 54 1425 343 524 890 1392 341 5019 Fragments arranged by size: 5019 1425 1392 890 524 343 341 54 MslI CAynn'nnrTG Cuts at: 518 1166 4214 4721 5033 6687 6852 7946 8305 Size: 648 3048 507 312 1654 165 1094 359 Cuts at: 8305 8464 518 Size: 159 2042 Fragments arranged by size: 3048 2042 1654 1094 648 507 359 312 165 159 MspA1I CmG'CkG Cuts at: 1606 2093 2102 3269 4023 4486 5809 7404 8081 Size: 487 9 1167 754 463 1323 1595 677 Cuts at: 8081 9229 9474 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1595 1323 1167 1148 754 677 487 463 245 9 NaeI GCC'GGC Cuts at: 2152 4610 5192 2152 Size: 2458 582 6948 Fragments arranged by size: 6948 2458 582 NarI GG'CG_CC Cuts at: 2300 2300 Size: 9988 NcoI C'CATG_G Cuts at: 513 1065 2004 4161 513 Size: 552 939 2157 6340 Fragments arranged by size: 6340 2157 939 552 NdeI CA'TA_TG Cuts at: 387 6410 387 Size: 6023 3965 NgoMIV G'CCGG_C Cuts at: 2150 4608 5190 2150 Size: 2458 582 6948 Fragments arranged by size: 6948 2458 582 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9988 NotI GC'GGCC_GC Cuts at: 7435 7435 Size: 9988 NsiI A_TGCA'T Cuts at: 3593 4718 3593 Size: 1125 8863 NspI r_CATG'y Cuts at: 1132 1229 2519 3595 5342 7133 7346 8891 1132 Size: 97 1290 1076 1747 1791 213 1545 2229 Fragments arranged by size: 2229 1791 1747 1545 1290 1076 213 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9988 PciI A'CATG_T Cuts at: 7129 8887 7129 Size: 1758 8230 PflMI CCAn_nnn'nTGG Cuts at: 1108 5056 1108 Size: 3948 6040 PfoI T'CCnGG_A Cuts at: 6219 6879 6219 Size: 660 9328 PmeI GTTT'AAAC Cuts at: 7292 7292 Size: 9988 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2426 2458 8057 8089 9570 9602 2426 Size: 32 5599 32 1481 32 2812 Fragments arranged by size: 5599 2812 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2188 2311 2585 5727 5843 2188 Size: 123 274 3142 116 6333 Fragments arranged by size: 6333 3142 274 123 116 PshAI GACnn'nnGTC Cuts at: 1613 6424 1613 Size: 4811 5177 PsiI TTA'TAA Cuts at: 7558 7558 Size: 9988 PspOMI G'GGCC_C Cuts at: 2969 4144 4540 6613 2969 Size: 1175 396 2073 6344 Fragments arranged by size: 6344 2073 1175 396 PspXI vC'TCGA_Gb Cuts at: 2136 2136 Size: 9988 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4101 4133 7208 7240 4101 Size: 32 3075 32 6849 Fragments arranged by size: 6849 3075 32 32 PstI C_TGCA'G Cuts at: 830 3790 4048 4491 4704 6350 7209 7340 830 Size: 2960 258 443 213 1646 859 131 3478 Fragments arranged by size: 3478 2960 1646 859 443 258 213 131 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9988 PvuII CAG'CTG Cuts at: 3269 4023 4486 3269 Size: 754 463 8771 Fragments arranged by size: 8771 754 463 SacI G_AGCT'C Cuts at: 3701 6288 3701 Size: 2587 7401 SacII CC_GC'GG Cuts at: 2094 2094 Size: 9988 SalI G'TCGA_C Cuts at: 1936 5388 7330 1936 Size: 3452 1942 4594 Fragments arranged by size: 4594 3452 1942 SanDI GG'GwC_CC Cuts at: 5727 5843 5727 Size: 116 9872 SapI GCTCTTCn'nnn_ Cuts at: 3891 5565 5978 3891 Size: 1674 413 7901 Fragments arranged by size: 7901 1674 413 SbfI CC_TGCA'GG Cuts at: 4704 7340 4704 Size: 2636 7352 ScaI AGT'ACT Cuts at: 8236 9948 8236 Size: 1712 8276 SexAI A'CCwGG_T Cuts at: 6503 7071 6503 Size: 568 9420 SfcI C'TryA_G Cuts at: 826 1045 2590 3123 3162 3529 3786 4044 4487 Size: 219 1545 533 39 367 257 258 443 Cuts at: 4487 4700 4806 6346 7205 7336 8471 9152 9343 Size: 213 106 1540 859 131 1135 681 191 Cuts at: 9343 826 Size: 1471 Fragments arranged by size: 1545 1540 1471 1135 859 681 533 443 367 258 257 219 213 191 131 106 39 SfoI GGC'GCC Cuts at: 2301 2301 Size: 9988 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9988 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9988 SmaI CCC'GGG Cuts at: 1872 5348 7315 1872 Size: 3476 1967 4545 Fragments arranged by size: 4545 3476 1967 SmlI C'TyrA_G Cuts at: 820 1017 1943 2136 3686 6275 6458 6764 7256 Size: 197 926 193 1550 2589 183 306 492 Cuts at: 7256 8092 8993 9255 820 Size: 836 901 262 1553 Fragments arranged by size: 2589 1553 1550 926 901 836 492 306 262 197 193 183 SnaBI TAC'GTA Cuts at: 493 7779 493 Size: 7286 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9988 SphI G_CATG'C Cuts at: 1132 7346 1132 Size: 6214 3774 SspI AAT'ATT Cuts at: 5 52 5323 7912 5 Size: 47 5271 2589 2081 Fragments arranged by size: 5271 2589 2081 47 StuI AGG'CCT Cuts at: 1517 3374 3705 4503 5632 6604 1517 Size: 1857 331 798 1129 972 4901 Fragments arranged by size: 4901 1857 1129 972 798 331 StyI C'CwwG_G Cuts at: 513 1065 2004 4008 4161 4602 5928 6861 7033 Size: 552 939 2004 153 441 1326 933 172 Cuts at: 7033 7427 513 Size: 394 3074 Fragments arranged by size: 3074 2004 1326 939 933 552 441 394 172 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2874 3188 4033 5207 8015 8032 8185 Size: 1255 1294 314 845 1174 2808 17 153 Cuts at: 8185 8370 8815 325 Size: 185 445 1498 Fragments arranged by size: 2808 1498 1294 1255 1174 845 445 314 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6624 8234 9946 Size: 274 80 33 51 1443 4647 1610 1712 Cuts at: 9946 96 Size: 138 Fragments arranged by size: 4647 1712 1610 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2627 2659 2854 2870 2886 2902 2977 3009 3793 Size: 32 195 16 16 16 75 32 784 Cuts at: 3793 3813 3825 3845 9948 9980 2627 Size: 20 12 20 6103 32 2635 Fragments arranged by size: 6103 2635 784 195 75 32 32 32 20 20 16 16 16 12 Tth111I GACn'n_nGTC Cuts at: 1316 2315 1316 Size: 999 8989 XbaI T'CTAG_A Cuts at: 4954 7324 4954 Size: 2370 7618 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5035 6840 5035 Size: 1805 8183 XhoI C'TCGA_G Cuts at: 1943 2136 1943 Size: 193 9795 XmaI C'CCGG_G Cuts at: 1870 5346 7313 1870 Size: 3476 1967 4545 Fragments arranged by size: 4545 3476 1967 XmnI GAAnn'nnTTC Cuts at: 2421 4391 6215 6483 8117 2421 Size: 1970 1824 268 1634 4292 Fragments arranged by size: 4292 1970 1824 1634 268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7796 276 Size: 53 83 186 936 6262 2468 Fragments arranged by size: 6262 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BsiWI BsmBI BstZ17I EcoRV FspAI NruI PacI PmlI RsrII SfiI SrfI SwaI