Restriction map for
pFN21AA1082
(Linear) (Six-base) MAPSORT of: KIAA1082.seq Check: 9565 from: 1 to: 5283
KIAA1082.orf
With 182 enzymes: SgfI *
December 5, 2008 09:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1143 5193 5283
Size: 1143 4050 90
Fragments arranged by size:
4050 1143 90
AccI GT'mk_AC
Cuts at: 0 926 1030 3384 4328 5283
Size: 926 104 2354 944 955
Fragments arranged by size:
2354 955 944 926 104
Acc65I G'GTAC_C
Cuts at: 0 2762 4955 5283
Size: 2762 2193 328
Fragments arranged by size:
2762 2193 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2521 2692 3584 5283
Size: 2521 171 892 1699
Fragments arranged by size:
2521 1699 892 171
AflIII A'CryG_T
Cuts at: 0 5124 5283
Size: 5124 159
AhdI GACnn_n'nnGTC
Cuts at: 0 3412 5283
Size: 3412 1871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 52 4865 5283
Size: 52 4813 418
Fragments arranged by size:
4813 418 52
AlwNI CAG_nnn'CTG
Cuts at: 0 1315 1333 4349 5283
Size: 1315 18 3016 934
Fragments arranged by size:
3016 1315 934 18
ApaI G_GGCC'C
Cuts at: 0 968 2143 2539 4612 5283
Size: 968 1175 396 2073 671
Fragments arranged by size:
2073 1175 968 671 396
ApoI r'AATT_y
Cuts at: 0 202 1380 1389 2507 4309 5155 5283
Size: 202 1178 9 1118 1802 846 128
Fragments arranged by size:
1802 1178 1118 846 202 128 9
AvaI C'yCGr_G
Cuts at: 0 131 1371 1886 2023 3341 5283
Size: 131 1240 515 137 1318 1942
Fragments arranged by size:
1942 1318 1240 515 137 131
AvrII C'CTAG_G
Cuts at: 0 5028 5283
Size: 5028 255
BanI G'GyrC_C
Cuts at: 0 174 294 2010 2721 2762 3501 4955 5245
Size: 174 120 1716 711 41 739 1454 290
Cuts at: 5245 5283
Size: 38
Fragments arranged by size:
1716 1454 739 711 290 174 120 41
38
BanII G_rGCy'C
Cuts at: 0 968 1696 2122 2143 2539 2690 4283 4612
Size: 968 728 426 21 396 151 1593 329
Cuts at: 4612 5061 5283
Size: 449 222
Fragments arranged by size:
1593 968 728 449 426 396 329 222
151 21
BbeI G_GCGC'C
Cuts at: 0 298 5283
Size: 298 4985
BbsI GAAGACnn'nnnn_
Cuts at: 0 3945 5111 5283
Size: 3945 1166 172
Fragments arranged by size:
3945 1166 172
BbvCI CC'TCA_GC
Cuts at: 0 66 251 3966 5283
Size: 66 185 3715 1317
Fragments arranged by size:
3715 1317 185 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2722 2756 4762 4796 5283
Size: 2722 34 2006 34 487
Fragments arranged by size:
2722 2006 487 34 34
BclI T'GATC_A
Cuts at: 0 542 4926 5283
Size: 542 4384 357
Fragments arranged by size:
4384 542 357
BfrBI ATG'CAT
Cuts at: 0 1586 2711 5283
Size: 1586 1125 2572
Fragments arranged by size:
2572 1586 1125
BglI GCCn_nnn'nGGC
Cuts at: 0 142 5283
Size: 142 5141
BglII A'GATC_T
Cuts at: 0 2946 3559 4208 4509 5283
Size: 2946 613 649 301 774
Fragments arranged by size:
2946 774 649 613 301
BlpI GC'TnA_GC
Cuts at: 0 2121 2558 5283
Size: 2121 437 2725
Fragments arranged by size:
2725 2121 437
Bme1580I G_kGCm'C
Cuts at: 0 179 968 2143 2539 4612 5283
Size: 179 789 1175 396 2073 671
Fragments arranged by size:
2073 1175 789 671 396 179
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 394 3135 5283
Size: 304 90 2741 2148
Fragments arranged by size:
2741 2148 304 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1441 2049 5283
Size: 1441 608 3234
Fragments arranged by size:
3234 1441 608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1790 2157 2418 2702 2776 3500 5075
Size: 304 1486 367 261 284 74 724 1575
Cuts at: 5075 5102 5283
Size: 27 181
Fragments arranged by size:
1575 1486 724 367 304 284 261 181
74 27
Bpu10I CC'TnA_GC
Cuts at: 0 66 251 1690 1695 3175 3966 5283
Size: 66 185 1439 5 1480 791 1317
Fragments arranged by size:
1480 1439 1317 791 185 66 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1702 4255 4438 4744 5236 5283
Size: 1702 2553 183 306 492 47
Fragments arranged by size:
2553 1702 492 306 183 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 287 2947 3399 3801 3875 4310 5283
Size: 287 2660 452 402 74 435 973
Fragments arranged by size:
2660 973 452 435 402 287 74
BsaBI GATnn'nnATC
Cuts at: 0 4726 5283
Size: 4726 557
BsaHI Gr'CG_yC
Cuts at: 0 295 5283
Size: 295 4988
BsaWI w'CCGG_w
Cuts at: 0 1769 2765 2825 5283
Size: 1769 996 60 2458
Fragments arranged by size:
2458 1769 996 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 351 381 849 879 1788 1808 1818 1838
Size: 351 30 468 30 909 20 10 20
Cuts at: 1838 2416 2446 3314 3344 5283
Size: 578 30 868 30 1939
Fragments arranged by size:
1939 909 868 578 468 351 30 30
30 30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 870 1968 2025 2082 2214 2254 2272 2488
Size: 870 1098 57 57 132 40 18 216
Cuts at: 2488 3506 3645 5005 5283
Size: 1018 139 1360 278
Fragments arranged by size:
1360 1098 1018 870 278 216 139 132
57 57 40 18
BseYI C'CCAG_C
Cuts at: 0 2014 2532 2568 2882 3834 4186 5009 5283
Size: 2014 518 36 314 952 352 823 274
Fragments arranged by size:
2014 952 823 518 352 314 274 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 1126 1517 2716 3649 5041 5283
Size: 500 626 391 1199 933 1392 242
Fragments arranged by size:
1392 1199 933 626 500 391 242
BsiEI CG_ry'CG
Cuts at: 0 1363 5283
Size: 1363 3920
BsiHKAI G_wGCw'C
Cuts at: 0 1422 1696 2519 3740 4283 4448 4743 5283
Size: 1422 274 823 1221 543 165 295 540
Fragments arranged by size:
1422 1221 823 543 540 295 274 165
BsmI GAATG_Cn'
Cuts at: 0 326 987 2752 5283
Size: 326 661 1765 2531
Fragments arranged by size:
2531 1765 661 326
Bsp1286I G_dGCh'C
Cuts at: 0 179 968 1422 1696 2122 2143 2519 2539
Size: 179 789 454 274 426 21 376 20
Cuts at: 2539 2690 3740 4283 4448 4612 4743 5061 5283
Size: 151 1050 543 165 164 131 318 222
Fragments arranged by size:
1050 789 543 454 426 376 318 274
222 179 165 164 151 131 21 20
BspEI T'CCGG_A
Cuts at: 0 2825 5283
Size: 2825 2458
BspHI T'CATG_A
Cuts at: 0 1851 4743 4809 4929 4941 5187 5259 5283
Size: 1851 2892 66 120 12 246 72 24
Fragments arranged by size:
2892 1851 246 120 72 66 24 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1143 3417 5058 5193 5283
Size: 1143 2274 1641 135 90
Fragments arranged by size:
2274 1641 1143 135 90
BsrBI CCG'CTC
Cuts at: 0 104 167 2171 2760 5283
Size: 104 63 2004 589 2523
Fragments arranged by size:
2523 2004 589 104 63
BsrDI GCAATG_nn'
Cuts at: 0 338 4016 5283
Size: 338 3678 1267
Fragments arranged by size:
3678 1267 338
BsrFI r'CCGG_y
Cuts at: 0 145 1358 2603 3170 3185 3498 5283
Size: 145 1213 1245 567 15 313 1785
Fragments arranged by size:
1785 1245 1213 567 313 145 15
BsrGI T'GTAC_A
Cuts at: 0 4619 5283
Size: 4619 664
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1477 3030 4312 5283
Size: 1477 1553 1282 971
Fragments arranged by size:
1553 1477 1282 971
BstYI r'GATC_y
Cuts at: 0 108 726 1304 1714 2946 3559 4128 4208
Size: 108 618 578 410 1232 613 569 80
Cuts at: 4208 4509 4871 5283
Size: 301 362 412
Fragments arranged by size:
1232 618 613 578 569 412 410 362
301 108 80
Bsu36I CC'TnA_GG
Cuts at: 0 3727 5283
Size: 3727 1556
BtgI C'CryG_G
Cuts at: 0 86 1816 2156 5283
Size: 86 1730 340 3127
Fragments arranged by size:
3127 1730 340 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 956 1421 5283
Size: 956 465 3862
Fragments arranged by size:
3862 956 465
BtsI GCAGTG_nn'
Cuts at: 0 56 2559 3201 3785 3842 5234 5283
Size: 56 2503 642 584 57 1392 49
Fragments arranged by size:
2503 1392 642 584 57 56 49
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3112 3147 5283
Size: 3112 35 2136
Fragments arranged by size:
3112 2136 35
DraIII CAC_nnn'GTG
Cuts at: 0 3196 5283
Size: 3196 2087
EaeI y'GGCC_r
Cuts at: 0 349 849 1239 2166 2631 2972 3187 5283
Size: 349 500 390 927 465 341 215 2096
Fragments arranged by size:
2096 927 500 465 390 349 341 215
EarI CTCTTCn'nnn_
Cuts at: 0 817 1410 1886 1986 2080 2928 3118 3200
Size: 817 593 476 100 94 848 190 82
Cuts at: 3200 3465 3560 3973 4650 4745 5283
Size: 265 95 413 677 95 538
Fragments arranged by size:
848 817 677 593 538 476 413 265
190 100 95 95 94 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 3503 3648 5131 5283
Size: 19 3484 145 1483 152
Fragments arranged by size:
3484 1483 152 145 19
EcoICRI GAG'CTC
Cuts at: 0 1694 4281 5283
Size: 1694 2587 1002
Fragments arranged by size:
2587 1694 1002
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1790 2157 2418 2521 2692 2702 2776
Size: 304 1486 367 261 103 171 10 74
Cuts at: 2776 3500 3584 5075 5102 5283
Size: 724 84 1491 27 181
Fragments arranged by size:
1491 1486 724 367 304 261 181 171
103 84 74 27 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 5149 5283
Size: 319 4830 134
Fragments arranged by size:
4830 319 134
EcoO109I rG'GnC_Cy
Cuts at: 0 115 183 306 580 964 965 2139 2257
Size: 115 68 123 274 384 1 1174 118
Cuts at: 2257 2536 2592 3722 3838 4609 5283
Size: 279 56 1130 116 771 674
Fragments arranged by size:
1174 1130 771 674 384 279 274 123
118 116 115 68 56 1
EcoRI G'AATT_C
Cuts at: 0 1380 2507 5155 5283
Size: 1380 1127 2648 128
Fragments arranged by size:
2648 1380 1127 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 301 2429 5283
Size: 301 2128 2854
Fragments arranged by size:
2854 2128 301
FseI GG_CCGG'CC
Cuts at: 0 2607 5283
Size: 2607 2676
FspI TGC'GCA
Cuts at: 0 127 5283
Size: 127 5156
HaeII r_GCGC'y
Cuts at: 0 298 5283
Size: 298 4985
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 351 383 877 909 2035 2067
Size: 93 32 226 32 494 32 1126 32
Cuts at: 2067 2202 2234 2416 2448 2826 2858 3137 3169
Size: 135 32 182 32 378 32 279 32
Cuts at: 3169 3398 3430 3544 3576 5098 5130 5283
Size: 229 32 114 32 1522 32 153
Fragments arranged by size:
1522 1126 494 378 279 229 226 182
153 135 114 93 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3385 5283
Size: 3385 1898
HindIII A'AGCT_T
Cuts at: 0 2406 3814 5283
Size: 2406 1408 1469
Fragments arranged by size:
2406 1469 1408
KasI G'GCGC_C
Cuts at: 0 294 5283
Size: 294 4989
KpnI G_GTAC'C
Cuts at: 0 2766 4959 5283
Size: 2766 2193 324
Fragments arranged by size:
2766 2193 324
MfeI C'AATT_G
Cuts at: 0 4200 4766 5283
Size: 4200 566 517
Fragments arranged by size:
4200 566 517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3875 4204 4875 5283
Size: 3875 329 671 408
Fragments arranged by size:
3875 671 408 329
MscI TGG'CCA
Cuts at: 0 351 1241 2633 2974 5283
Size: 351 890 1392 341 2309
Fragments arranged by size:
2309 1392 890 351 341
MslI CAynn'nnrTG
Cuts at: 0 2209 2716 3028 4682 4847 5283
Size: 2209 507 312 1654 165 436
Fragments arranged by size:
2209 1654 507 436 312 165
MspA1I CmG'CkG
Cuts at: 0 88 97 1264 2018 2481 3804 5283
Size: 88 9 1167 754 463 1323 1479
Fragments arranged by size:
1479 1323 1167 754 463 88 9
NaeI GCC'GGC
Cuts at: 0 147 2605 3187 5283
Size: 147 2458 582 2096
Fragments arranged by size:
2458 2096 582 147
NarI GG'CG_CC
Cuts at: 0 295 5283
Size: 295 4988
NcoI C'CATG_G
Cuts at: 0 2156 5283
Size: 2156 3127
NdeI CA'TA_TG
Cuts at: 0 4405 5283
Size: 4405 878
NgoMIV G'CCGG_C
Cuts at: 0 145 2603 3185 5283
Size: 145 2458 582 2098
Fragments arranged by size:
2458 2098 582 145
NsiI A_TGCA'T
Cuts at: 0 1588 2713 5283
Size: 1588 1125 2570
Fragments arranged by size:
2570 1588 1125
NspI r_CATG'y
Cuts at: 0 514 1590 3337 5128 5283
Size: 514 1076 1747 1791 155
Fragments arranged by size:
1791 1747 1076 514 155
PciI A'CATG_T
Cuts at: 0 5124 5283
Size: 5124 159
PflMI CCAn_nnn'nTGG
Cuts at: 0 3051 5283
Size: 3051 2232
PfoI T'CCnGG_A
Cuts at: 0 4214 4874 5283
Size: 4214 660 409
Fragments arranged by size:
4214 660 409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 5283
Size: 421 32 4830
Fragments arranged by size:
4830 421 32
PpuMI rG'GwC_Cy
Cuts at: 0 183 306 580 3722 3838 5283
Size: 183 123 274 3142 116 1445
Fragments arranged by size:
3142 1445 274 183 123 116
PshAI GACnn'nnGTC
Cuts at: 0 4419 5283
Size: 4419 864
PspOMI G'GGCC_C
Cuts at: 0 964 2139 2535 4608 5283
Size: 964 1175 396 2073 675
Fragments arranged by size:
2073 1175 964 675 396
PspXI vC'TCGA_Gb
Cuts at: 0 131 5283
Size: 131 5152
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2096 2128 5203 5235 5283
Size: 2096 32 3075 32 48
Fragments arranged by size:
3075 2096 48 32 32
PstI C_TGCA'G
Cuts at: 0 1785 2043 2486 2699 4345 5204 5283
Size: 1785 258 443 213 1646 859 79
Fragments arranged by size:
1785 1646 859 443 258 213 79
PvuII CAG'CTG
Cuts at: 0 1264 2018 2481 5283
Size: 1264 754 463 2802
Fragments arranged by size:
2802 1264 754 463
SacI G_AGCT'C
Cuts at: 0 1696 4283 5283
Size: 1696 2587 1000
Fragments arranged by size:
2587 1696 1000
SacII CC_GC'GG
Cuts at: 0 89 5283
Size: 89 5194
SalI G'TCGA_C
Cuts at: 0 3383 5283
Size: 3383 1900
SanDI GG'GwC_CC
Cuts at: 0 3722 3838 5283
Size: 3722 116 1445
Fragments arranged by size:
3722 1445 116
SapI GCTCTTCn'nnn_
Cuts at: 0 1886 3560 3973 5283
Size: 1886 1674 413 1310
Fragments arranged by size:
1886 1674 1310 413
SbfI CC_TGCA'GG
Cuts at: 0 2699 5283
Size: 2699 2584
SexAI A'CCwGG_T
Cuts at: 0 4498 5066 5283
Size: 4498 568 217
Fragments arranged by size:
4498 568 217
SfcI C'TryA_G
Cuts at: 0 585 1118 1157 1524 1781 2039 2482 2695
Size: 585 533 39 367 257 258 443 213
Cuts at: 2695 2801 4341 5200 5283
Size: 106 1540 859 83
Fragments arranged by size:
1540 859 585 533 443 367 258 257
213 106 83 39
SfoI GGC'GCC
Cuts at: 0 296 5283
Size: 296 4987
SmaI CCC'GGG
Cuts at: 0 3343 5283
Size: 3343 1940
SmlI C'TyrA_G
Cuts at: 0 131 1681 4270 4453 4759 5251 5283
Size: 131 1550 2589 183 306 492 32
Fragments arranged by size:
2589 1550 492 306 183 131 32
SspI AAT'ATT
Cuts at: 0 3318 5283
Size: 3318 1965
StuI AGG'CCT
Cuts at: 0 1369 1700 2498 3627 4599 5283
Size: 1369 331 798 1129 972 684
Fragments arranged by size:
1369 1129 972 798 684 331
StyI C'CwwG_G
Cuts at: 0 2003 2156 2597 3923 4856 5028 5283
Size: 2003 153 441 1326 933 172 255
Fragments arranged by size:
2003 1326 933 441 255 172 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 869 1183 2028 3202 5283
Size: 869 314 845 1174 2081
Fragments arranged by size:
2081 1174 869 845 314
TatI w'GTAC_w
Cuts at: 0 4619 5283
Size: 4619 664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 622 654 849 865 881 897 972 1004
Size: 622 32 195 16 16 16 75 32
Cuts at: 1004 1788 1808 1820 1840 5283
Size: 784 20 12 20 3443
Fragments arranged by size:
3443 784 622 195 75 32 32 20
20 16 16 16 12
Tth111I GACn'n_nGTC
Cuts at: 0 310 5283
Size: 310 4973
XbaI T'CTAG_A
Cuts at: 0 2949 5283
Size: 2949 2334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3030 4835 5283
Size: 3030 1805 448
Fragments arranged by size:
3030 1805 448
XhoI C'TCGA_G
Cuts at: 0 131 5283
Size: 131 5152
XmaI C'CCGG_G
Cuts at: 0 3341 5283
Size: 3341 1942
XmnI GAAnn'nnTTC
Cuts at: 0 416 2386 4210 4478 5283
Size: 416 1970 1824 268 805
Fragments arranged by size:
1970 1824 805 416 268
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlfI AlwNI
ApaI ApoI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
SacI SacII SalI SanDI SapI SbfI SexAI SfcI
SfoI SmaI SmlI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI AloI ApaLI
AscI AseI AsiSI BaeI BamHI BciVI BmgBI BmtI
BsaAI BsiWI BsmBI BssHII BssSI BstAPI BstBI BstEII
BstZ17I ClaI DraI DrdI EagI EcoRV FspAI HpaI
MluI NheI NotI NruI PacI PasI PmeI PmlI
PsiI PvuI RsrII ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI ZraI