(Circular) (Six-base) MAPSORT of: pFN21AB7689.seq Check: 3380 from: 1 to: 5752 pFN21AB7689 5752 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2328 3095 1937 Size: 391 767 4594 Fragments arranged by size: 4594 767 391 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2305 3815 5199 5467 1632 Size: 673 1510 1384 268 1917 Fragments arranged by size: 1917 1510 1384 673 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 4651 5628 1203 Size: 3448 977 1327 Fragments arranged by size: 3448 1327 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2915 1184 Size: 1731 4021 AlwNI CAG_nnn'CTG Cuts at: 2916 3495 5067 2916 Size: 579 1572 3601 Fragments arranged by size: 3601 1572 579 ApaLI G'TGCA_C Cuts at: 3809 4965 3809 Size: 1156 4596 ApoI r'AATT_y Cuts at: 1493 2242 2260 3061 3287 3439 5548 1493 Size: 749 18 801 226 152 2109 1697 Fragments arranged by size: 2109 1697 801 749 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2808 2994 3077 1870 Size: 73 125 740 186 83 4545 Fragments arranged by size: 4545 740 186 125 83 73 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2899 3073 4528 618 Size: 325 217 627 1112 174 1455 1842 Fragments arranged by size: 1842 1455 1112 627 325 217 174 BanII G_rGCy'C Cuts at: 1391 2076 2661 3071 1391 Size: 685 585 410 4072 Fragments arranged by size: 4072 685 585 410 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2400 2679 928 Size: 971 501 279 4001 Fragments arranged by size: 4001 971 501 279 BbvCI CC'TCA_GC Cuts at: 2792 2792 Size: 5752 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3943 3977 1903 Size: 34 50 34 1922 34 3678 Fragments arranged by size: 3678 1922 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BfrBI ATG'CAT Cuts at: 2122 2122 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2758 4363 136 Size: 107 122 71 631 605 1086 1605 1525 Fragments arranged by size: 1605 1525 1086 631 605 122 107 71 BglII A'GATC_T Cuts at: 2673 5747 2673 Size: 3074 2678 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2724 3813 4969 1790 Size: 934 1089 1156 2573 Fragments arranged by size: 2573 1156 1089 934 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2185 3483 4441 Size: 416 53 83 399 24 768 1298 958 Cuts at: 4441 442 Size: 1753 Fragments arranged by size: 1753 1298 958 768 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2852 4412 1239 Size: 821 792 1560 2579 Fragments arranged by size: 2579 1560 821 792 Bpu10I CC'TnA_GC Cuts at: 2792 2792 Size: 5752 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2060 3877 4742 5040 2060 Size: 1817 865 298 2772 Fragments arranged by size: 2772 1817 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2984 4415 882 Size: 617 1485 1431 2219 Fragments arranged by size: 2219 1485 1431 617 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 5746 5746 Size: 5752 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2837 3559 3941 4576 Size: 53 83 186 936 1304 722 382 635 Cuts at: 4576 275 Size: 1451 Fragments arranged by size: 1451 1304 936 722 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2860 2896 4185 4583 4857 5004 5415 2860 Size: 36 1289 398 274 147 411 3197 Fragments arranged by size: 3197 1289 411 398 274 147 36 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2850 2880 2897 2927 1178 Size: 30 165 30 1447 30 17 30 4003 Fragments arranged by size: 4003 1447 165 30 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2900 1186 Size: 871 843 4038 Fragments arranged by size: 4038 871 843 BseYI C'CCAG_C Cuts at: 2723 2959 4955 5451 2723 Size: 236 1996 496 3024 Fragments arranged by size: 3024 1996 496 236 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 3963 4991 1540 Size: 275 186 1201 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2661 3813 3898 4969 2661 Size: 1152 85 1071 3444 Fragments arranged by size: 3444 1152 1071 85 BsmI GAATG_Cn' Cuts at: 2340 3263 3356 2340 Size: 923 93 4736 Fragments arranged by size: 4736 923 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2661 2724 3071 3813 3898 4969 Size: 399 286 585 63 347 742 85 1071 Cuts at: 4969 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 585 399 347 286 85 63 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 3641 5371 3641 Size: 1730 4022 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2829 3107 844 Size: 1985 278 3489 Fragments arranged by size: 3489 1985 278 BsrBI CCG'CTC Cuts at: 1394 1823 3639 1394 Size: 429 1816 3507 Fragments arranged by size: 3507 1816 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4247 4421 65 Size: 1183 2999 174 1396 Fragments arranged by size: 2999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 2641 4396 4583 1700 Size: 110 198 633 1755 187 2869 Fragments arranged by size: 2869 1755 633 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5752 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 2617 2872 1184 Size: 1433 255 4064 Fragments arranged by size: 4064 1433 255 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5752 BstYI r'GATC_y Cuts at: 1972 2109 2673 3082 3449 3834 3851 5303 5389 Size: 137 564 409 367 385 17 1452 86 Cuts at: 5389 5401 5747 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 564 409 385 367 346 137 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2033 513 Size: 552 968 4232 Fragments arranged by size: 4232 968 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2742 487 Size: 683 56 1516 3497 Fragments arranged by size: 3497 1516 683 56 BtsI GCAGTG_nn' Cuts at: 3268 4062 4082 3268 Size: 794 20 4938 Fragments arranged by size: 4938 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2870 4759 809 Size: 507 1554 1889 1802 Fragments arranged by size: 1889 1802 1554 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2750 2978 3199 Size: 54 1425 50 275 18 920 228 221 Cuts at: 3199 4088 5717 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 920 889 275 228 221 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 1537 Size: 275 1387 4090 Fragments arranged by size: 4090 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2727 2759 3682 1353 Size: 1374 32 923 3423 Fragments arranged by size: 3423 1374 923 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2580 2776 4339 4713 4859 387 Size: 1064 138 991 196 1563 374 146 1280 Fragments arranged by size: 1563 1280 1064 991 374 196 146 138 EcoICRI GAG'CTC Cuts at: 2659 2659 Size: 5752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2305 2852 3815 4412 5199 5467 Size: 393 428 245 547 963 597 787 268 Cuts at: 5467 1239 Size: 1524 Fragments arranged by size: 1524 963 787 597 547 428 393 268 245 EcoRI G'AATT_C Cuts at: 2260 3061 3439 2260 Size: 801 378 4573 Fragments arranged by size: 4573 801 378 EcoRV GAT'ATC Cuts at: 3025 3025 Size: 5752 FspI TGC'GCA Cuts at: 2024 4258 2024 Size: 2234 3518 FspAI rTGC'GCAy Cuts at: 2024 2024 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 4899 1678 Size: 3221 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373 Size: 32 135 32 2821 32 42 32 2626 Fragments arranged by size: 2821 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3342 669 Size: 988 281 1158 246 3079 Fragments arranged by size: 3079 1158 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5752 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 3351 3351 Size: 5752 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2082 2877 4866 5050 1305 Size: 777 795 1989 184 2007 Fragments arranged by size: 2007 1989 795 777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3930 Fragments arranged by size: 3930 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3710 4069 4228 518 Size: 648 843 1701 359 159 2042 Fragments arranged by size: 2042 1701 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3168 3845 4993 5238 1606 Size: 1562 677 1148 245 2120 Fragments arranged by size: 2120 1562 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5200 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NsiI A_TGCA'T Cuts at: 2124 2124 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 2029 2126 3110 4655 1132 Size: 97 800 97 984 1545 2229 Fragments arranged by size: 2229 1545 984 800 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5752 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 2232 1108 Size: 1124 4628 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PspXI vC'TCGA_Gb Cuts at: 2808 2808 Size: 5752 PstI C_TGCA'G Cuts at: 830 2673 3104 830 Size: 1843 431 3478 Fragments arranged by size: 3478 1843 431 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 SacI G_AGCT'C Cuts at: 2661 2661 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SfcI C'TryA_G Cuts at: 826 1045 2669 3100 4235 4916 5107 826 Size: 219 1624 431 1135 681 191 1471 Fragments arranged by size: 1624 1471 1135 681 431 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 2070 2996 3079 1872 Size: 198 926 83 4545 Fragments arranged by size: 4545 926 198 83 SmlI C'TyrA_G Cuts at: 820 1017 1943 2075 2808 3856 4757 5019 820 Size: 197 926 132 733 1048 901 262 1553 Fragments arranged by size: 1553 1048 926 901 733 262 197 132 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 2029 2126 3110 1132 Size: 897 97 984 3774 Fragments arranged by size: 3774 984 897 97 SspI AAT'ATT Cuts at: 5 52 2773 3676 5 Size: 47 2721 903 2081 Fragments arranged by size: 2721 2081 903 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5752 StyI C'CwwG_G Cuts at: 513 1065 3191 513 Size: 552 2126 3074 Fragments arranged by size: 3074 2126 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2127 3044 3779 3796 3949 4134 4579 Size: 1255 547 917 735 17 153 185 445 Cuts at: 4579 325 Size: 1498 Fragments arranged by size: 1498 1255 917 735 547 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3998 5710 96 Size: 274 80 33 51 1443 2021 1712 138 Fragments arranged by size: 2021 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5712 5744 5712 Size: 32 5720 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XhoI C'TCGA_G Cuts at: 1943 2808 1943 Size: 865 4887 XmaI C'CCGG_G Cuts at: 1870 2068 2994 3077 1870 Size: 198 926 83 4545 Fragments arranged by size: 4545 926 198 83 XmnI GAAnn'nnTTC Cuts at: 3881 3881 Size: 5752 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI