Restriction map for
pFN21AB6319
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaII AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmFI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI TspRI Tth111I
Van91I XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 151 CGCG CG^CG
AcsI 400 GAATTT [GA]^AATT[CT]
AfaI 685 GTAC GT^AC
AlwNI 491 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 189 GGGCCC GGGCC^C
ApoI 400 GAATTT [GA]^AATT[CT]
Asp700I 40 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 26 CCCGAG C^[CT]CG[GA]G
BalI 579 TGGCCA TGG^CCA
BanI 169 GGCACC G^G[CT][GA]CC
BanII 189 GGGCCC G[GA]GC[CT]^C
BclI 648 TGATCA T^GATCA
BmyI 189 GGGCCC G[AGT]GC[ACT]^C
BsaWI 59 ACCGGA [AT]^CCGG[AT]
BseRI 123 CTCCTC GAGGAG(10/8)
BsgI 310 GTGCAG GTGCAG(16/14)
BsmBI 94 GAGACG CGTCTC(1/5)
BsmI 540 GAATGC GAATGC(1/-1)
BsoBI 26 CCCGAG C^[CT]CG[GA]G
Bsp1286I 189 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 684 TGTACA T^GTACA
BspMI 204 GCAGGT ACCTGC(4/8)
BsrGI 684 TGTACA T^GTACA
BstAPI 303 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 659 CATCC GGATG(2/0)
BstUI 151 CGCG CG^CG
DpnII 649 GATC ^GATC
DraII 189 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 579 TGGCCA [CT]^GGCC[GA]
EarI 762 GAAGAG CTCTTC(1/4)
EcoO109I 189 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 230 CCAAGG C^C[AT][AT]GG
FbaI 648 TGATCA T^GATCA
FokI 659 CATCC GGATG(9/13)
HgaI 96 GACGC GACGC(5/10)
HphI 651 TCACC GGTGA(8/7)
Ksp632I 762 GAAGAG CTCTTC(1/4)
MboI 649 GATC ^GATC
MluNI 579 TGGCCA TGG^CCA
MscI 579 TGGCCA TGG^CCA
MseI 679 TTAA T^TAA
MvnI 151 CGCG CG^CG
NdeII 649 GATC ^GATC
NspI 714 ACATGC [GA]CATG^[CT]
PleI 665 GACTC GAGTC(4/5)
RsaI 685 GTAC GT^AC
Sau3AI 649 GATC ^GATC
SspBI 684 TGTACA T^GTACA
SspI 418 AATATT AAT^ATT
StyI 230 CCAAGG C^C[AT][AT]GG
TaqI 510 TCGA T^CGA
TfiI 504 GAATC G^A[AT]TC
Tru9I 679 TTAA T^TAA
Tsp45I 331 GTGAC ^GT[GC]AC
Tsp509I 401 AATT ^AATT
TthHB8I 510 TCGA T^CGA
XbaI 273 TCTAGA T^CTAGA
XmnI 40 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AocI 83 CCTCAGG CC^T[ACGT]AGG
587 CCTTAGG
BfaI 274 CTAG C^TAG
445 CTAG
BpmI 295 CTCCAG CTGGAG(16/14)
488 CTCCAG
BsmAI 94 GAGAC GTCTC(1/5)
3 GTCTC
Bsu36I 83 CCTCAGG CC^T[ACGT]AGG
587 CCTTAGG
CfoI 202 GCGC GCG^C
549 GCGC
Cfr13I 189 GGGCC G^G[ACGT]CC
190 GGCCC
Eco81I 83 CCTCAGG CC^T[ACGT]AGG
587 CCTTAGG
FauI 149 CCCGC CCCGC(4/6)
187 GCGGG
HhaI 202 GCGC GCG^C
549 GCGC
HinP1I 202 GCGC G^CGC
549 GCGC
HinfI 504 GAATC G^A[ACGT]TC
665 GACTC
MaeI 274 CTAG C^TAG
445 CTAG
NlaIII 369 CATG CATG^
715 CATG
Sau96I 189 GGGCC G^G[ACGT]CC
190 GGCCC
SmlI 557 CTCAAG C^T[CT][GA]AG
669 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BsrI BssKI BstNI
Cac8I DdeI EcoRII Fnu4HI HaeIII
HapII HpaII ItaI MaeIII MboII
MnlI MspI MvaI MwoI NciI
NlaIV ScrFI TseI