(Circular) (Six-base) MAPSORT of: pFN21AB6319.seq Check: 7709 from: 1 to: 5485 pFN21AB6319 5485 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2200 2200 Size: 5485 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3295 278 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3548 4932 5200 1632 Size: 1916 1384 268 1917 Fragments arranged by size: 1917 1916 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 4384 5361 1203 Size: 3181 977 1327 Fragments arranged by size: 3181 1327 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 4214 4214 Size: 5485 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2404 1184 Size: 1220 4265 AlwNI CAG_nnn'CTG Cuts at: 2501 3228 4800 2501 Size: 727 1572 3186 Fragments arranged by size: 3186 1572 727 ApaI G_GGCC'C Cuts at: 2198 2198 Size: 5485 ApaLI G'TGCA_C Cuts at: 3542 4698 3542 Size: 1156 4329 ApoI r'AATT_y Cuts at: 1493 2405 2794 3020 3172 5281 1493 Size: 912 389 226 152 2109 1697 Fragments arranged by size: 2109 1697 912 389 226 152 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2031 2810 1870 Size: 73 88 779 4545 Fragments arranged by size: 4545 779 88 73 BamHI G'GATC_C Cuts at: 2815 3182 2815 Size: 367 5118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2806 4261 618 Size: 325 217 627 387 632 1455 1842 Fragments arranged by size: 1842 1455 632 627 387 325 217 BanII G_rGCy'C Cuts at: 1391 2198 2804 1391 Size: 807 606 4072 Fragments arranged by size: 4072 807 606 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4514 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3676 3710 1903 Size: 34 1739 34 3678 Fragments arranged by size: 3678 1739 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4587 3377 Size: 1210 4275 BclI T'GATC_A Cuts at: 1548 2653 1548 Size: 1105 4380 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4096 136 Size: 107 122 71 631 605 2424 1525 Fragments arranged by size: 2424 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2902 2902 Size: 5485 Bme1580I G_kGCm'C Cuts at: 1790 2198 3546 4702 1790 Size: 408 1348 1156 2573 Fragments arranged by size: 2573 1348 1156 408 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2473 3216 4174 Size: 416 53 83 399 24 1056 743 958 Cuts at: 4174 442 Size: 1753 Fragments arranged by size: 1753 1056 958 743 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2478 4145 1239 Size: 1046 193 1667 2579 Fragments arranged by size: 2579 1667 1046 193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2547 2659 3610 4475 4773 2547 Size: 112 951 865 298 3259 Fragments arranged by size: 3259 951 865 298 112 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4148 882 Size: 617 2649 2219 Fragments arranged by size: 2649 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 493 Size: 711 2072 2702 Fragments arranged by size: 2702 2072 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3292 3674 4309 275 Size: 53 83 186 936 1759 382 635 1451 Fragments arranged by size: 1759 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2064 3918 4316 4590 4737 5148 2064 Size: 1854 398 274 147 411 2401 Fragments arranged by size: 2401 1854 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 1178 Size: 30 165 30 708 30 4522 Fragments arranged by size: 4522 708 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2119 1186 Size: 933 4552 BseYI C'CCAG_C Cuts at: 4688 5184 4688 Size: 496 4989 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2336 1163 Size: 573 600 4312 Fragments arranged by size: 4312 600 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2935 3696 4724 1540 Size: 275 186 934 761 1028 2301 Fragments arranged by size: 2301 1028 934 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3546 3631 4702 3546 Size: 85 1071 4329 Fragments arranged by size: 4329 1071 85 BsmI GAATG_Cn' Cuts at: 2551 2996 3089 2551 Size: 445 93 4947 Fragments arranged by size: 4947 445 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2093 2093 Size: 5485 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2804 3546 3631 4702 1391 Size: 399 408 606 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 606 408 399 85 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 3374 5104 3374 Size: 1730 3755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2200 2840 844 Size: 1356 640 3489 Fragments arranged by size: 3489 1356 640 BsrBI CCG'CTC Cuts at: 1394 1823 3372 1394 Size: 429 1549 3507 Fragments arranged by size: 3507 1549 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3980 4154 65 Size: 1183 2732 174 1396 Fragments arranged by size: 2732 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4129 4316 1700 Size: 110 2319 187 2869 Fragments arranged by size: 2869 2319 187 110 BsrGI T'GTAC_A Cuts at: 96 2689 96 Size: 2593 2892 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5485 BssSI C'ACGA_G Cuts at: 3545 4557 3545 Size: 1012 4473 BstAPI GCAn_nnn'nTGC Cuts at: 794 2314 794 Size: 1520 3965 BstBI TT'CG_AA Cuts at: 4289 4289 Size: 5485 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5485 BstYI r'GATC_y Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480 Size: 843 367 385 17 1452 86 12 346 Cuts at: 5480 1972 Size: 1977 Fragments arranged by size: 1977 1452 843 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2089 2593 2089 Size: 504 4981 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4933 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4746 Fragments arranged by size: 4746 683 56 BtsI GCAGTG_nn' Cuts at: 3001 3795 3815 3001 Size: 794 20 4671 Fragments arranged by size: 4671 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5450 DraI TTT'AAA Cuts at: 2789 2789 Size: 5485 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4492 809 Size: 507 3176 1802 Fragments arranged by size: 3176 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2584 2932 3821 Size: 54 1425 50 275 18 754 348 889 Cuts at: 3821 5450 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 754 348 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2932 1537 Size: 275 1120 4090 Fragments arranged by size: 4090 1120 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2762 3415 1353 Size: 1409 653 3423 Fragments arranged by size: 3423 1409 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4072 4446 4592 387 Size: 1064 138 2483 374 146 1280 Fragments arranged by size: 2483 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2478 3548 4145 4932 5200 1239 Size: 393 653 193 1070 597 787 268 1524 Fragments arranged by size: 1524 1070 787 653 597 393 268 193 EcoO109I rG'GnC_Cy Cuts at: 2195 2195 Size: 5485 EcoRI G'AATT_C Cuts at: 2794 3172 2794 Size: 378 5107 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2546 2546 Size: 5485 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 4632 1678 Size: 2954 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2254 2286 2645 2677 4126 Size: 32 135 32 682 32 359 32 1449 Cuts at: 4126 4158 4200 4232 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1449 682 359 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2829 3075 669 Size: 988 281 891 246 3079 Fragments arranged by size: 3079 988 891 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5485 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 3084 3084 Size: 5485 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2051 4599 4783 1305 Size: 746 2548 184 2007 Fragments arranged by size: 2548 2007 746 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2586 10 Size: 54 1425 343 754 2909 Fragments arranged by size: 2909 1425 754 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3443 3802 3961 518 Size: 648 2277 359 159 2042 Fragments arranged by size: 2277 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2901 3578 4726 4971 1606 Size: 1295 677 1148 245 2120 Fragments arranged by size: 2120 1295 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4933 NdeI CA'TA_TG Cuts at: 387 387 Size: 5485 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2723 2843 4388 1132 Size: 97 1494 120 1545 2229 Fragments arranged by size: 2229 1545 1494 120 97 PasI CC'CwG_GG Cuts at: 1348 2055 1348 Size: 707 4778 PciI A'CATG_T Cuts at: 4384 4384 Size: 5485 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5485 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 5067 5099 3554 Size: 32 1481 32 3940 Fragments arranged by size: 3940 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 2741 3055 2741 Size: 314 5171 PspOMI G'GGCC_C Cuts at: 2194 2194 Size: 5485 PstI C_TGCA'G Cuts at: 830 2837 830 Size: 2007 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SbfI CC_TGCA'GG Cuts at: 2837 2837 Size: 5485 ScaI AGT'ACT Cuts at: 3733 5445 3733 Size: 1712 3773 SfcI C'TryA_G Cuts at: 826 1045 2833 3968 4649 4840 826 Size: 219 1788 1135 681 191 1471 Fragments arranged by size: 1788 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2812 1872 Size: 940 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2562 2674 3589 4490 4752 820 Size: 197 926 619 112 915 901 262 1553 Fragments arranged by size: 1553 926 915 901 619 262 197 112 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 2425 3409 5 Size: 47 2373 984 2081 Fragments arranged by size: 2373 2081 984 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5485 StyI C'CwwG_G Cuts at: 513 1065 2235 2924 513 Size: 552 1170 689 3074 Fragments arranged by size: 3074 1170 689 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3512 3529 3682 3867 4312 325 Size: 1255 1932 17 153 185 445 1498 Fragments arranged by size: 1932 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2689 3731 5443 Size: 274 80 33 51 1443 712 1042 1712 Cuts at: 5443 96 Size: 138 Fragments arranged by size: 1712 1443 1042 712 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 2648 2680 5445 5477 2111 Size: 32 505 32 2765 32 2119 Fragments arranged by size: 2765 2119 505 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2278 2821 2278 Size: 543 4942 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5485 XmaI C'CCGG_G Cuts at: 1870 2810 1870 Size: 940 4545 XmnI GAAnn'nnTTC Cuts at: 2049 3614 2049 Size: 1565 3920 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3293 276 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI