Restriction map for
pFN21AB6319
(Linear) (Six-base) MAPSORT of: KIBB6319.seq Check: 7423 from: 1 to: 780
KIBB6319.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 195 780
Size: 195 585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 399 780
Size: 399 381
AlwNI CAG_nnn'CTG
Cuts at: 0 496 780
Size: 496 284
ApaI G_GGCC'C
Cuts at: 0 193 780
Size: 193 587
ApoI r'AATT_y
Cuts at: 0 400 780
Size: 400 380
AvaI C'yCGr_G
Cuts at: 0 26 780
Size: 26 754
BanI G'GyrC_C
Cuts at: 0 169 780
Size: 169 611
BanII G_rGCy'C
Cuts at: 0 193 780
Size: 193 587
BclI T'GATC_A
Cuts at: 0 648 780
Size: 648 132
Bme1580I G_kGCm'C
Cuts at: 0 193 780
Size: 193 587
BmrI ACTGGGnnnn_n'
Cuts at: 0 468 780
Size: 468 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 473 780
Size: 280 193 307
Fragments arranged by size:
307 280 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 654 780
Size: 542 112 126
Fragments arranged by size:
542 126 112
BsaWI w'CCGG_w
Cuts at: 0 59 780
Size: 59 721
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 780
Size: 106 30 644
Fragments arranged by size:
644 106 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 114 780
Size: 114 666
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 780
Size: 331 449
BsmI GAATG_Cn'
Cuts at: 0 546 780
Size: 546 234
BsmBI CGTCTCn'nnnn_
Cuts at: 0 88 780
Size: 88 692
Bsp1286I G_dGCh'C
Cuts at: 0 193 780
Size: 193 587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 195 780
Size: 195 585
BsrGI T'GTAC_A
Cuts at: 0 684 780
Size: 684 96
BstAPI GCAn_nnn'nTGC
Cuts at: 0 309 780
Size: 309 471
Bsu36I CC'TnA_GG
Cuts at: 0 84 588 780
Size: 84 504 192
Fragments arranged by size:
504 192 84
EaeI y'GGCC_r
Cuts at: 0 579 780
Size: 579 201
EarI CTCTTCn'nnn_
Cuts at: 0 757 780
Size: 757 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 473 780
Size: 280 193 307
Fragments arranged by size:
307 280 193
EcoO109I rG'GnC_Cy
Cuts at: 0 190 780
Size: 190 590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 541 780
Size: 541 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 249 281 640 672 780
Size: 249 32 359 32 108
Fragments arranged by size:
359 249 108 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 780
Size: 46 734
MscI TGG'CCA
Cuts at: 0 581 780
Size: 581 199
NspI r_CATG'y
Cuts at: 0 718 780
Size: 718 62
PasI CC'CwG_GG
Cuts at: 0 50 780
Size: 50 730
PsiI TTA'TAA
Cuts at: 0 736 780
Size: 736 44
PspOMI G'GGCC_C
Cuts at: 0 189 780
Size: 189 591
SmlI C'TyrA_G
Cuts at: 0 557 669 780
Size: 557 112 111
Fragments arranged by size:
557 112 111
SspI AAT'ATT
Cuts at: 0 420 780
Size: 420 360
StyI C'CwwG_G
Cuts at: 0 230 780
Size: 230 550
TatI w'GTAC_w
Cuts at: 0 684 780
Size: 684 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 643 675 780
Size: 106 32 505 32 105
Fragments arranged by size:
505 106 105 32 32
XbaI T'CTAG_A
Cuts at: 0 273 780
Size: 273 507
XmnI GAAnn'nnTTC
Cuts at: 0 44 780
Size: 44 736
Enzymes that do cut:
AarI AlfI AlwNI ApaI ApoI AvaI BanI BanII
BclI Bme1580I BmrI BpmI BpuEI BsaWI BsaXI BseRI
BsgI BsmI BsmBI Bsp1286I BspMI BsrGI BstAPI Bsu36I
EaeI EarI Eco57MI EcoO109I FalI Hin4I MmeI MscI
NspI PasI PsiI PspOMI SmlI SspI StyI TatI
TstI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BseYI BsiEI BsiHKAI BsiWI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII Tth111I XcmI XhoI XmaI
ZraI