(Circular) (Six-base) MAPSORT of: pFN21AA0522.seq Check: 2940 from: 1 to: 9169 pFN21AA0522 9169 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2487 2487 Size: 9169 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6979 278 Size: 53 83 186 936 5443 2468 Fragments arranged by size: 5443 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4915 6512 1937 Size: 2978 1597 4594 Fragments arranged by size: 4594 2978 1597 Acc65I G'GTAC_C Cuts at: 6490 6490 Size: 9169 AclI AA'CG_TT Cuts at: 1563 7296 7669 1563 Size: 5733 373 3063 Fragments arranged by size: 5733 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3526 3920 3978 4567 6157 7232 8616 8884 Size: 1894 394 58 589 1590 1075 1384 268 Cuts at: 8884 1632 Size: 1917 Fragments arranged by size: 1917 1894 1590 1384 1075 589 394 268 58 AfeI AGC'GCT Cuts at: 2948 2948 Size: 9169 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8972 AflIII A'CryG_T Cuts at: 1203 4455 4686 8068 9045 1203 Size: 3252 231 3382 977 1327 Fragments arranged by size: 3382 3252 1327 977 231 AgeI A'CCGG_T Cuts at: 2438 4357 8000 2438 Size: 1919 3643 3607 Fragments arranged by size: 3643 3607 1919 AhdI GACnn_n'nnGTC Cuts at: 4846 7898 4846 Size: 3052 6117 AleI CACnn'nnGTG Cuts at: 1166 4358 1166 Size: 3192 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5450 1184 Size: 4266 4903 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2306 2338 3276 3308 2306 Size: 32 938 32 8167 Fragments arranged by size: 8167 938 32 32 AlwNI CAG_nnn'CTG Cuts at: 2110 2113 2278 3956 4327 5156 5803 6912 8484 Size: 3 165 1678 371 829 647 1109 1572 Cuts at: 8484 2110 Size: 2795 Fragments arranged by size: 2795 1678 1572 1109 829 647 371 165 3 ApaI G_GGCC'C Cuts at: 2025 2855 3462 3928 3987 4020 5655 5834 5997 Size: 830 607 466 59 33 1635 179 163 Cuts at: 5997 6158 6221 6402 2025 Size: 161 63 181 4792 Fragments arranged by size: 4792 1635 830 607 466 181 179 163 161 63 59 33 ApaLI G'TGCA_C Cuts at: 7226 8382 7226 Size: 1156 8013 ApoI r'AATT_y Cuts at: 1493 2725 4418 5028 5113 6478 6704 6856 8965 Size: 1232 1693 610 85 1365 226 152 2109 Cuts at: 8965 1493 Size: 1697 Fragments arranged by size: 2109 1697 1693 1365 1232 610 226 152 85 AseI AT'TA_AT Cuts at: 160 1032 7723 160 Size: 872 6691 1606 Fragments arranged by size: 6691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9169 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2035 2251 2337 2542 3632 3690 Size: 73 83 9 216 86 205 1090 58 Cuts at: 3690 3731 4155 4779 6494 1870 Size: 41 424 624 1715 4545 Fragments arranged by size: 4545 1715 1090 624 424 216 205 86 83 73 58 41 9 AvrII C'CTAG_G Cuts at: 4423 4423 Size: 9169 BamHI G'GATC_C Cuts at: 2039 6224 6499 6866 2039 Size: 4185 275 367 4342 Fragments arranged by size: 4342 4185 367 275 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 2651 3251 3263 3643 Size: 325 217 627 579 285 600 12 380 Cuts at: 3643 4945 6028 6490 7945 618 Size: 1302 1083 462 1455 1842 Fragments arranged by size: 1842 1455 1302 1083 627 600 579 462 380 325 285 217 12 BanII G_rGCy'C Cuts at: 1391 2025 2051 2642 2855 2884 3035 3294 3462 Size: 634 26 591 213 29 151 259 168 Cuts at: 3462 3928 3987 4020 4109 5409 5624 5655 5834 Size: 466 59 33 89 1300 215 31 179 Cuts at: 5834 5918 5997 6158 6221 6245 6402 6488 1391 Size: 84 79 161 63 24 157 86 4072 Fragments arranged by size: 4072 1300 634 591 466 259 215 213 179 168 161 157 151 89 86 84 79 63 59 33 31 29 26 24 BbeI G_GCGC'C Cuts at: 2370 2655 3647 2370 Size: 285 992 7892 Fragments arranged by size: 7892 992 285 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3702 4776 4987 928 Size: 971 1803 1074 211 5110 Fragments arranged by size: 5110 1803 1074 971 211 BbvCI CC'TCA_GC Cuts at: 4397 5938 4397 Size: 1541 7628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4905 4939 5225 5259 6448 6482 7360 Size: 34 2968 34 286 34 1189 34 878 Cuts at: 7360 7394 1903 Size: 34 3678 Fragments arranged by size: 3678 2968 1189 878 286 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4328 7061 8271 4328 Size: 2733 1210 5226 Fragments arranged by size: 5226 2733 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2534 5760 Size: 107 122 71 631 605 337 525 3226 Cuts at: 5760 5782 6021 7780 136 Size: 22 239 1759 1525 Fragments arranged by size: 3226 1759 1525 631 605 525 337 239 122 107 71 22 BglII A'GATC_T Cuts at: 5046 9164 5046 Size: 4118 5051 BlpI GC'TnA_GC Cuts at: 2618 2816 2883 3121 3529 6586 2618 Size: 198 67 238 408 3057 5201 Fragments arranged by size: 5201 3057 408 238 198 67 Bme1580I G_kGCm'C Cuts at: 1790 2025 2855 2893 3268 3462 3928 3987 4020 Size: 235 830 38 375 194 466 59 33 Cuts at: 4020 5655 5834 5997 6031 6158 6221 6402 7230 Size: 1635 179 163 34 127 63 181 828 Cuts at: 7230 8386 1790 Size: 1156 2573 Fragments arranged by size: 2573 1635 1156 830 828 466 375 235 194 181 179 163 127 63 59 38 34 33 BmgBI CAC'GTC Cuts at: 1318 2344 2464 4456 1318 Size: 1026 120 1992 6031 Fragments arranged by size: 6031 1992 1026 120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2284 2464 4014 Size: 416 53 83 399 24 867 180 1550 Cuts at: 4014 4023 5806 5978 6262 6417 6900 7858 442 Size: 9 1783 172 284 155 483 958 1753 Fragments arranged by size: 1783 1753 1550 958 867 483 416 399 284 180 172 155 83 53 24 9 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9169 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3544 3544 Size: 9169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2165 2411 2626 2687 2818 2882 2883 Size: 857 69 246 215 61 131 64 1 Cuts at: 2883 3035 3375 3512 4093 4403 5186 5309 5352 Size: 152 340 137 581 310 783 123 43 Cuts at: 5352 5399 5780 6066 6123 7829 1239 Size: 47 381 286 57 1706 2579 Fragments arranged by size: 2579 1706 857 783 581 381 340 310 286 246 215 152 137 131 123 69 64 61 57 47 43 1 Bpu10I CC'TnA_GC Cuts at: 2771 3041 3591 4397 5938 6159 2771 Size: 270 550 806 1541 221 5781 Fragments arranged by size: 5781 1541 806 550 270 221 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3404 4121 7294 8159 8457 3404 Size: 717 3173 865 298 4116 Fragments arranged by size: 4116 3173 865 717 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2279 4118 4776 5433 7832 882 Size: 617 780 1839 658 657 2399 2219 Fragments arranged by size: 2399 2219 1839 780 658 657 617 BsaAI yAC'GTr Cuts at: 493 1204 6960 493 Size: 711 5756 2702 Fragments arranged by size: 5756 2702 711 BsaBI GATnn'nnATC Cuts at: 9163 9163 Size: 9169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 2412 2652 3644 Size: 53 83 186 936 834 45 240 992 Cuts at: 3644 6976 7358 7993 275 Size: 3332 382 635 1451 Fragments arranged by size: 3332 1451 992 936 834 635 382 240 186 83 53 45 BsaWI w'CCGG_w Cuts at: 2438 4357 4515 4629 7602 8000 8274 8421 8832 Size: 1919 158 114 2973 398 274 147 411 Cuts at: 8832 2438 Size: 2775 Fragments arranged by size: 2973 2775 1919 411 398 274 158 147 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2306 2336 2375 Size: 30 165 30 597 30 276 30 39 Cuts at: 2375 2405 2629 2659 3008 3038 3276 3306 3380 Size: 30 224 30 349 30 238 30 74 Cuts at: 3380 3410 3464 3494 4124 4154 4475 4505 5835 Size: 30 54 30 630 30 321 30 1330 Cuts at: 5835 5865 1178 Size: 30 4482 Fragments arranged by size: 4482 1330 630 597 349 321 276 238 224 165 74 54 39 30 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2207 2945 2948 2951 3203 3485 4138 Size: 967 54 738 3 3 252 282 653 Cuts at: 4138 4480 4664 5588 5596 5686 1186 Size: 342 184 924 8 90 4669 Fragments arranged by size: 4669 967 924 738 653 342 282 252 184 90 54 8 3 3 BseYI C'CCAG_C Cuts at: 2121 2494 3486 3602 3949 3964 3970 4031 4227 Size: 373 992 116 347 15 6 61 196 Cuts at: 4227 4609 4697 5195 5859 5886 5907 5911 6129 Size: 382 88 498 664 27 21 4 218 Cuts at: 6129 6212 8372 8868 2121 Size: 83 2160 496 2422 Fragments arranged by size: 2422 2160 992 664 498 496 382 373 347 218 196 116 88 83 61 27 21 15 6 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2189 2204 2465 4877 5273 6071 6371 Size: 573 453 15 261 2412 396 798 300 Cuts at: 6371 1163 Size: 3961 Fragments arranged by size: 3961 2412 798 573 453 396 300 261 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6619 7380 8408 1540 Size: 275 186 4618 761 1028 2301 Fragments arranged by size: 4618 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2642 2777 4109 7230 7315 8386 2642 Size: 135 1332 3121 85 1071 3425 Fragments arranged by size: 3425 3121 1332 1071 135 85 BsmI GAATG_Cn' Cuts at: 6680 6773 6680 Size: 93 9076 BsmBI CGTCTCn'nnnn_ Cuts at: 4446 5602 4446 Size: 1156 8013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2051 2642 2777 2855 2884 2893 Size: 399 235 26 591 135 78 29 9 Cuts at: 2893 3035 3268 3294 3462 3928 3987 4020 4109 Size: 142 233 26 168 466 59 33 89 Cuts at: 4109 5409 5624 5655 5834 5918 5997 6031 6158 Size: 1300 215 31 179 84 79 34 127 Cuts at: 6158 6221 6245 6402 6488 7230 7315 8386 1391 Size: 63 24 157 86 742 85 1071 2174 Fragments arranged by size: 2174 1300 1071 742 591 466 399 235 233 215 179 168 157 142 135 127 89 86 85 84 79 78 63 59 34 33 31 29 26 26 24 9 BspEI T'CCGG_A Cuts at: 4515 4629 8832 4515 Size: 114 4203 4852 Fragments arranged by size: 4852 4203 114 BspHI T'CATG_A Cuts at: 7058 8788 7058 Size: 1730 7439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2193 2286 2487 2997 5238 6524 844 Size: 1235 114 93 201 510 2241 1286 3489 Fragments arranged by size: 3489 2241 1286 1235 510 201 114 93 BsrBI CCG'CTC Cuts at: 1394 1823 2407 2425 2608 3109 3831 4336 4387 Size: 429 584 18 183 501 722 505 51 Cuts at: 4387 7056 1394 Size: 2669 3507 Fragments arranged by size: 3507 2669 722 584 505 501 429 183 51 18 BsrDI GCAATG_nn' Cuts at: 65 1248 5241 7664 7838 65 Size: 1183 3993 2423 174 1396 Fragments arranged by size: 3993 2423 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2438 2533 2654 2811 3276 3627 4184 Size: 110 628 95 121 157 465 351 557 Cuts at: 4184 4357 4401 4431 5208 6015 7813 8000 1700 Size: 173 44 30 777 807 1798 187 2869 Fragments arranged by size: 2869 1798 807 777 628 557 465 351 187 173 157 121 110 95 44 30 BsrGI T'GTAC_A Cuts at: 96 4689 6114 96 Size: 4593 1425 3151 Fragments arranged by size: 4593 3151 1425 BssHII G'CGCG_C Cuts at: 1925 2238 1925 Size: 313 8856 BssSI C'ACGA_G Cuts at: 2486 4616 5084 7229 8241 2486 Size: 2130 468 2145 1012 3414 Fragments arranged by size: 3414 2145 2130 1012 468 BstAPI GCAn_nnn'nTGC Cuts at: 794 2110 794 Size: 1316 7853 BstBI TT'CG_AA Cuts at: 7973 7973 Size: 9169 BstEII G'GTnAC_C Cuts at: 1184 5020 1184 Size: 3836 5333 BstXI CCAn_nnnn'nTGG Cuts at: 4027 4501 5744 5978 4027 Size: 474 1243 234 7218 Fragments arranged by size: 7218 1243 474 234 BstYI r'GATC_y Cuts at: 1972 2039 4498 4951 5046 6224 6499 6866 7251 Size: 67 2459 453 95 1178 275 367 385 Cuts at: 7251 7268 8720 8806 8818 9164 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2459 1977 1452 1178 453 385 367 346 275 95 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2918 2918 Size: 9169 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2209 2233 2591 2600 2623 Size: 552 939 57 148 24 358 9 23 Cuts at: 2623 3255 3813 3889 4494 5755 5977 6376 6462 Size: 632 558 76 605 1261 222 399 86 Cuts at: 6462 513 Size: 3220 Fragments arranged by size: 3220 1261 939 632 605 558 552 399 358 222 148 86 76 57 24 23 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5415 5945 487 Size: 683 56 4189 530 3711 Fragments arranged by size: 4189 3711 683 530 56 BtsI GCAGTG_nn' Cuts at: 2770 6104 6685 7479 7499 2770 Size: 3334 581 794 20 4440 Fragments arranged by size: 4440 3334 794 581 20 ClaI AT'CG_AT Cuts at: 1554 3324 1554 Size: 1770 7399 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9134 DraI TTT'AAA Cuts at: 6473 6473 Size: 9169 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8176 809 Size: 507 6860 1802 Fragments arranged by size: 6860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2594 2691 5746 Size: 54 1425 50 275 18 764 97 3055 Cuts at: 5746 6017 6073 6230 6616 7505 9134 8 Size: 271 56 157 386 889 1629 43 Fragments arranged by size: 3055 1629 1425 889 764 386 275 271 157 97 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6616 1537 Size: 275 4804 4090 Fragments arranged by size: 4804 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4156 4298 5006 5111 5704 5877 7099 1353 Size: 2803 142 708 105 593 173 1222 3423 Fragments arranged by size: 3423 2803 1222 708 593 173 142 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2673 3068 5541 7756 8130 8276 Size: 1064 138 1084 395 2473 2215 374 146 Cuts at: 8276 387 Size: 1280 Fragments arranged by size: 2473 2215 1280 1084 1064 395 374 146 138 EcoICRI GAG'CTC Cuts at: 2640 4107 2640 Size: 1467 7702 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2165 2411 2626 2687 2818 2882 Size: 393 464 69 246 215 61 131 64 Cuts at: 2882 2883 3035 3375 3512 3526 3920 3978 4093 Size: 1 152 340 137 14 394 58 115 Cuts at: 4093 4403 4567 5186 5309 5352 5399 5780 6066 Size: 310 164 619 123 43 47 381 286 Cuts at: 6066 6123 6157 7232 7829 8616 8884 1239 Size: 57 34 1075 597 787 268 1524 Fragments arranged by size: 1524 1075 787 619 597 464 394 393 381 340 310 286 268 246 215 164 152 137 131 123 115 69 64 61 58 57 47 43 34 14 1 EcoNI CCTnn'n_nnAGG Cuts at: 2189 2189 Size: 9169 EcoO109I rG'GnC_Cy Cuts at: 2021 2022 2227 2851 2852 2956 3458 3728 3894 Size: 1 205 624 1 104 502 270 166 Cuts at: 3894 3924 3925 3983 3984 5651 5652 5800 5831 Size: 30 1 58 1 1667 1 148 31 Cuts at: 5831 5993 5994 6154 6155 6217 6218 6399 2021 Size: 162 1 160 1 62 1 181 4791 Fragments arranged by size: 4791 1667 624 502 270 205 181 166 162 160 148 104 62 58 31 30 1 1 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 2725 4418 5113 6478 6856 2725 Size: 1693 695 1365 378 5038 Fragments arranged by size: 5038 1693 1365 695 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5065 5671 5065 Size: 606 8563 FseI GG_CCGG'CC Cuts at: 3631 6019 3631 Size: 2388 6781 FspI TGC'GCA Cuts at: 7675 7675 Size: 9169 HaeII r_GCGC'y Cuts at: 1678 2370 2655 2950 3647 8316 1678 Size: 692 285 295 697 4669 2531 Fragments arranged by size: 4669 2531 697 692 295 285 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2038 2070 2629 Size: 32 135 32 428 32 6 32 559 Cuts at: 2629 2661 3380 3412 3464 3496 3827 3859 4475 Size: 32 719 32 52 32 331 32 616 Cuts at: 4475 4507 4832 4864 5533 5565 5771 5803 7810 Size: 32 325 32 669 32 206 32 2007 Cuts at: 7810 7842 7884 7916 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2007 719 669 616 559 428 331 325 206 135 52 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 4759 4916 6513 6759 669 Size: 988 281 2821 157 1597 246 3079 Fragments arranged by size: 3079 2821 1597 988 281 246 157 HindIII A'AGCT_T Cuts at: 748 748 Size: 9169 HpaI GTT'AAC Cuts at: 6759 6759 Size: 9169 KasI G'GCGC_C Cuts at: 2366 2651 3643 2366 Size: 285 992 7892 Fragments arranged by size: 7892 992 285 KpnI G_GTAC'C Cuts at: 6494 6494 Size: 9169 MfeI C'AATT_G Cuts at: 6768 6768 Size: 9169 MluI A'CGCG_T Cuts at: 9045 9045 Size: 9169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3186 4019 4440 4861 5250 5262 5625 8283 Size: 1881 833 421 421 389 12 363 2658 Cuts at: 8283 8467 1305 Size: 184 2007 Fragments arranged by size: 2658 2007 1881 833 421 421 389 363 184 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 5748 10 Size: 54 1425 343 3916 3431 Fragments arranged by size: 3916 3431 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3170 4358 5742 5976 7127 7486 7645 Size: 648 2004 1188 1384 234 1151 359 159 Cuts at: 7645 518 Size: 2042 Fragments arranged by size: 2042 2004 1384 1188 1151 648 359 234 159 MspA1I CmG'CkG Cuts at: 1606 2110 2125 2155 2203 2211 2593 2602 2617 Size: 504 15 30 48 8 382 9 15 Cuts at: 2617 2780 2824 2887 3490 3859 4210 4591 4987 Size: 163 44 63 603 369 351 381 396 Cuts at: 4987 5911 6385 6585 7262 8410 8655 1606 Size: 924 474 200 677 1148 245 2120 Fragments arranged by size: 2120 1148 924 677 603 504 474 396 382 381 369 351 245 200 163 63 48 44 30 15 15 9 8 NaeI GCC'GGC Cuts at: 2535 2656 3629 4403 6017 2535 Size: 121 973 774 1614 5687 Fragments arranged by size: 5687 1614 973 774 121 NarI GG'CG_CC Cuts at: 2367 2652 3644 2367 Size: 285 992 7892 Fragments arranged by size: 7892 992 285 NcoI C'CATG_G Cuts at: 513 1065 2004 3255 3889 4494 5977 6376 513 Size: 552 939 1251 634 605 1483 399 3306 Fragments arranged by size: 3306 1483 1251 939 634 605 552 399 NdeI CA'TA_TG Cuts at: 387 3298 387 Size: 2911 6258 NgoMIV G'CCGG_C Cuts at: 2533 2654 3627 4401 6015 2533 Size: 121 973 774 1614 5687 Fragments arranged by size: 5687 1614 973 774 121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9169 NotI GC'GGCC_GC Cuts at: 6616 6616 Size: 9169 NruI TCG'CGA Cuts at: 2508 2508 Size: 9169 NspI r_CATG'y Cuts at: 1132 1229 3142 3169 4690 5101 5810 6527 8072 Size: 97 1913 27 1521 411 709 717 1545 Cuts at: 8072 1132 Size: 2229 Fragments arranged by size: 2229 1913 1545 1521 717 709 411 97 27 PasI CC'CwG_GG Cuts at: 1348 2500 2877 3622 5990 1348 Size: 1152 377 745 2368 4527 Fragments arranged by size: 4527 2368 1152 745 377 PciI A'CATG_T Cuts at: 4686 8068 4686 Size: 3382 5787 PflMI CCAn_nnn'nTGG Cuts at: 1108 4561 1108 Size: 3453 5716 PfoI T'CCnGG_A Cuts at: 2427 2633 4380 5202 2427 Size: 206 1747 822 6394 Fragments arranged by size: 6394 1747 822 206 PmeI GTTT'AAAC Cuts at: 6473 6473 Size: 9169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2276 2306 2308 2338 3276 3308 7238 7270 8751 Size: 30 2 30 938 32 3930 32 1481 Cuts at: 8751 8783 2276 Size: 32 2662 Fragments arranged by size: 3930 2662 1481 938 32 32 32 30 30 2 PpuMI rG'GwC_Cy Cuts at: 2227 3728 3894 2227 Size: 1501 166 7502 Fragments arranged by size: 7502 1501 166 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9169 PsiI TTA'TAA Cuts at: 6739 6739 Size: 9169 PspOMI G'GGCC_C Cuts at: 2021 2851 3458 3924 3983 4016 5651 5830 5993 Size: 830 607 466 59 33 1635 179 163 Cuts at: 5993 6154 6217 6398 2021 Size: 161 63 181 4792 Fragments arranged by size: 4792 1635 830 607 466 181 179 163 161 63 59 33 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3200 3232 3200 Size: 32 9137 PstI C_TGCA'G Cuts at: 830 2994 3884 4139 5152 6521 830 Size: 2164 890 255 1013 1369 3478 Fragments arranged by size: 3478 2164 1369 1013 890 255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9169 PvuII CAG'CTG Cuts at: 2110 2155 2203 2617 2824 3490 3859 5911 2110 Size: 45 48 414 207 666 369 2052 5368 Fragments arranged by size: 5368 2052 666 414 369 207 48 45 SacI G_AGCT'C Cuts at: 2642 4109 2642 Size: 1467 7702 SacII CC_GC'GG Cuts at: 2212 2594 2603 2212 Size: 382 9 8778 Fragments arranged by size: 8778 382 9 SalI G'TCGA_C Cuts at: 1936 4914 6511 1936 Size: 2978 1597 4594 Fragments arranged by size: 4594 2978 1597 SanDI GG'GwC_CC Cuts at: 2227 3728 3894 2227 Size: 1501 166 7502 Fragments arranged by size: 7502 1501 166 SapI GCTCTTCn'nnn_ Cuts at: 5111 5111 Size: 9169 SbfI CC_TGCA'GG Cuts at: 2994 6521 2994 Size: 3527 5642 ScaI AGT'ACT Cuts at: 7417 9129 7417 Size: 1712 7457 SexAI A'CCwGG_T Cuts at: 5155 5155 Size: 9169 SfcI C'TryA_G Cuts at: 826 1045 2990 3880 4135 5148 6517 7652 8333 Size: 219 1945 890 255 1013 1369 1135 681 Cuts at: 8333 8524 826 Size: 191 1471 Fragments arranged by size: 1945 1471 1369 1135 1013 890 681 255 219 191 SfoI GGC'GCC Cuts at: 2368 2653 3645 2368 Size: 285 992 7892 Fragments arranged by size: 7892 992 285 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9169 SgrAI Cr'CCGG_yG Cuts at: 1700 2654 4357 1700 Size: 954 1703 6512 Fragments arranged by size: 6512 1703 954 SmaI CCC'GGG Cuts at: 1872 2028 2037 2253 2339 2544 3692 3733 6496 Size: 156 9 216 86 205 1148 41 2763 Cuts at: 6496 1872 Size: 4545 Fragments arranged by size: 4545 2763 1148 216 205 156 86 41 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 3383 4100 7273 8174 8436 820 Size: 197 926 1440 717 3173 901 262 1553 Fragments arranged by size: 3173 1553 1440 926 901 717 262 197 SnaBI TAC'GTA Cuts at: 493 6960 493 Size: 6467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9169 SphI G_CATG'C Cuts at: 1132 5810 6527 1132 Size: 4678 717 3774 Fragments arranged by size: 4678 3774 717 SrfI GCCC'GGGC Cuts at: 2253 2253 Size: 9169 SspI AAT'ATT Cuts at: 5 52 7093 5 Size: 47 7041 2081 Fragments arranged by size: 7041 2081 47 StuI AGG'CCT Cuts at: 1517 4395 5687 1517 Size: 2878 1292 4999 Fragments arranged by size: 4999 2878 1292 StyI C'CwwG_G Cuts at: 513 1065 2004 2952 3255 3889 4423 4494 5340 Size: 552 939 948 303 634 534 71 846 Cuts at: 5340 5821 5977 6009 6376 6435 6608 513 Size: 481 156 32 367 59 173 3074 Fragments arranged by size: 3074 948 939 846 634 552 534 481 367 303 173 156 71 59 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2183 6046 6198 6307 6379 7196 7213 Size: 1255 603 3863 152 109 72 817 17 Cuts at: 7213 7366 7551 7996 325 Size: 153 185 445 1498 Fragments arranged by size: 3863 1498 1255 817 603 445 185 153 152 109 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4689 5577 6114 Size: 274 80 33 51 1443 2712 888 537 Cuts at: 6114 7415 9127 96 Size: 1301 1712 138 Fragments arranged by size: 2712 1712 1443 1301 888 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5835 5867 9129 9161 5835 Size: 32 3262 32 5843 Fragments arranged by size: 5843 3262 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9169 XbaI T'CTAG_A Cuts at: 6505 6505 Size: 9169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2621 2842 5741 5756 2621 Size: 221 2899 15 6034 Fragments arranged by size: 6034 2899 221 15 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9169 XmaI C'CCGG_G Cuts at: 1870 2026 2035 2251 2337 2542 3690 3731 6494 Size: 156 9 216 86 205 1148 41 2763 Cuts at: 6494 1870 Size: 4545 Fragments arranged by size: 4545 2763 1148 216 205 156 86 41 9 XmnI GAAnn'nnTTC Cuts at: 4442 7298 4442 Size: 2856 6313 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6977 276 Size: 53 83 186 936 5443 2468 Fragments arranged by size: 5443 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FspAI NsiI PacI PmlI PspXI RsrII SfiI SwaI