Restriction map for
pFN21AA1023
(Linear) (Six-base) MAPSORT of: KIAA1023.seq Check: 8233 from: 1 to: 1890
KIAA1023.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AatII G_ACGT'C
Cuts at: 0 1826 1890
Size: 1826 64
AccI GT'mk_AC
Cuts at: 0 221 1890
Size: 221 1669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 469 484 757 1024 1435 1706 1890
Size: 141 328 15 273 267 411 271 184
Fragments arranged by size:
411 328 273 271 267 184 141 15
AgeI A'CCGG_T
Cuts at: 0 1675 1890
Size: 1675 215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1618 1890
Size: 1618 272
AlwNI CAG_nnn'CTG
Cuts at: 0 39 81 369 735 1038 1068 1283 1890
Size: 39 42 288 366 303 30 215 607
Fragments arranged by size:
607 366 303 288 215 42 39 30
AvaI C'yCGr_G
Cuts at: 0 1655 1890
Size: 1655 235
BamHI G'GATC_C
Cuts at: 0 372 458 1890
Size: 372 86 1432
Fragments arranged by size:
1432 372 86
BanI G'GyrC_C
Cuts at: 0 320 496 652 1803 1890
Size: 320 176 156 1151 87
Fragments arranged by size:
1151 320 176 156 87
BanII G_rGCy'C
Cuts at: 0 716 871 1256 1325 1552 1576 1890
Size: 716 155 385 69 227 24 314
Fragments arranged by size:
716 385 314 227 155 69 24
BbeI G_GCGC'C
Cuts at: 0 324 656 1890
Size: 324 332 1234
Fragments arranged by size:
1234 332 324
BbsI GAAGACnn'nnnn_
Cuts at: 0 532 652 1890
Size: 532 120 1238
Fragments arranged by size:
1238 532 120
BbvCI CC'TCA_GC
Cuts at: 0 823 1890
Size: 823 1067
BlpI GC'TnA_GC
Cuts at: 0 760 1890
Size: 760 1130
Bme1580I G_kGCm'C
Cuts at: 0 684 1562 1647 1890
Size: 684 878 85 243
Fragments arranged by size:
878 684 243 85
BmgBI CAC'GTC
Cuts at: 0 294 1890
Size: 294 1596
BmrI ACTGGGnnnn_n'
Cuts at: 0 1383 1890
Size: 1383 507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 496 574 862 1108 1120 1890
Size: 346 150 78 288 246 12 770
Fragments arranged by size:
770 346 288 246 150 78 12
Bpu10I CC'TnA_GC
Cuts at: 0 688 823 1890
Size: 688 135 1067
Fragments arranged by size:
1067 688 135
BsaI GGTCTCn'nnnn_
Cuts at: 0 992 1890
Size: 992 898
BsaAI yAC'GTr
Cuts at: 0 279 1890
Size: 279 1611
BsaBI GATnn'nnATC
Cuts at: 0 550 1890
Size: 550 1340
BsaHI Gr'CG_yC
Cuts at: 0 119 321 653 1364 1739 1823 1890
Size: 119 202 332 711 375 84 67
Fragments arranged by size:
711 375 332 202 119 84 67
BsaWI w'CCGG_w
Cuts at: 0 619 1675 1890
Size: 619 1056 215
Fragments arranged by size:
1056 619 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 1087 1117 1729 1759 1813 1843
Size: 94 30 963 30 612 30 54 30
Cuts at: 1843 1890
Size: 47
Fragments arranged by size:
963 612 94 54 47 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 589 1135 1138 1143 1156 1171 1330
Size: 343 246 546 3 5 13 15 159
Cuts at: 1330 1624 1725 1734 1890
Size: 294 101 9 156
Fragments arranged by size:
546 343 294 246 159 156 101 15
13 9 5 3
BseYI C'CCAG_C
Cuts at: 0 45 376 1257 1270 1522 1890
Size: 45 331 881 13 252 368
Fragments arranged by size:
881 368 331 252 45 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 922 1624 1890
Size: 301 621 702 266
Fragments arranged by size:
702 621 301 266
BsiEI CG_ry'CG
Cuts at: 0 15 1890
Size: 15 1875
BsiHKAI G_wGCw'C
Cuts at: 0 716 766 871 1256 1552 1890
Size: 716 50 105 385 296 338
Fragments arranged by size:
716 385 338 296 105 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 592 985 1355 1868 1890
Size: 592 393 370 513 22
Fragments arranged by size:
592 513 393 370 22
Bsp1286I G_dGCh'C
Cuts at: 0 684 716 766 871 1256 1325 1552 1562
Size: 684 32 50 105 385 69 227 10
Cuts at: 1562 1576 1647 1890
Size: 14 71 243
Fragments arranged by size:
684 385 243 227 105 71 69 50
32 14 10
BsrBI CCG'CTC
Cuts at: 0 1014 1337 1636 1890
Size: 1014 323 299 254
Fragments arranged by size:
1014 323 299 254
BsrDI GCAATG_nn'
Cuts at: 0 271 1853 1890
Size: 271 1582 37
Fragments arranged by size:
1582 271 37
BsrFI r'CCGG_y
Cuts at: 0 350 1675 1890
Size: 350 1325 215
Fragments arranged by size:
1325 350 215
BsrGI T'GTAC_A
Cuts at: 0 1400 1890
Size: 1400 490
BssSI C'ACGA_G
Cuts at: 0 950 973 1890
Size: 950 23 917
Fragments arranged by size:
950 917 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1038 1068 1283 1890
Size: 1038 30 215 607
Fragments arranged by size:
1038 607 215 30
BstYI r'GATC_y
Cuts at: 0 372 390 458 1890
Size: 372 18 68 1432
Fragments arranged by size:
1432 372 68 18
Bsu36I CC'TnA_GG
Cuts at: 0 1785 1890
Size: 1785 105
BtgI C'CryG_G
Cuts at: 0 381 557 1327 1367 1589 1890
Size: 381 176 770 40 222 301
Fragments arranged by size:
770 381 301 222 176 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1563 1890
Size: 1563 327
BtsI GCAGTG_nn'
Cuts at: 0 484 684 1281 1890
Size: 484 200 597 609
Fragments arranged by size:
609 597 484 200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1781 1816 1890
Size: 1781 35 74
Fragments arranged by size:
1781 74 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1849 1890
Size: 1849 41
EaeI y'GGCC_r
Cuts at: 0 12 1297 1370 1890
Size: 12 1285 73 520
Fragments arranged by size:
1285 520 73 12
EagI C'GGCC_G
Cuts at: 0 12 1890
Size: 12 1878
EarI CTCTTCn'nnn_
Cuts at: 0 160 280 536 716 1004 1205 1353 1772
Size: 160 120 256 180 288 201 148 419
Cuts at: 1772 1890
Size: 118
Fragments arranged by size:
419 288 256 201 180 160 148 120
118
EcoICRI GAG'CTC
Cuts at: 0 714 869 1254 1550 1890
Size: 714 155 385 296 340
Fragments arranged by size:
714 385 340 296 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 346 469 484 496 574 757 862
Size: 141 205 123 15 12 78 183 105
Cuts at: 862 1024 1108 1120 1435 1706 1890
Size: 162 84 12 315 271 184
Fragments arranged by size:
315 271 205 184 183 162 141 123
105 84 78 15 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 739 1090 1890
Size: 328 411 351 800
Fragments arranged by size:
800 411 351 328
EcoO109I rG'GnC_Cy
Cuts at: 0 78 744 1890
Size: 78 666 1146
Fragments arranged by size:
1146 666 78
HaeII r_GCGC'y
Cuts at: 0 186 324 656 1890
Size: 186 138 332 1234
Fragments arranged by size:
1234 332 186 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 1534 1566 1729 1761 1813 1845
Size: 159 32 1343 32 163 32 52 32
Cuts at: 1845 1890
Size: 45
Fragments arranged by size:
1343 163 159 52 45 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1827 1890
Size: 1827 63
KasI G'GCGC_C
Cuts at: 0 320 652 1890
Size: 320 332 1238
Fragments arranged by size:
1238 332 320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1865 1890
Size: 1865 25
MspA1I CmG'CkG
Cuts at: 0 341 383 1035 1329 1369 1890
Size: 341 42 652 294 40 521
Fragments arranged by size:
652 521 341 294 42 40
NarI GG'CG_CC
Cuts at: 0 321 653 1890
Size: 321 332 1237
Fragments arranged by size:
1237 332 321
NcoI C'CATG_G
Cuts at: 0 557 1890
Size: 557 1333
NspI r_CATG'y
Cuts at: 0 109 1890
Size: 109 1781
PasI CC'CwG_GG
Cuts at: 0 208 633 1890
Size: 208 425 1257
Fragments arranged by size:
1257 425 208
PfoI T'CCnGG_A
Cuts at: 0 368 697 707 1890
Size: 368 329 10 1183
Fragments arranged by size:
1183 368 329 10
PmlI CAC'GTG
Cuts at: 0 279 1890
Size: 279 1611
PpuMI rG'GwC_Cy
Cuts at: 0 744 1890
Size: 744 1146
PshAI GACnn'nnGTC
Cuts at: 0 118 217 1890
Size: 118 99 1673
Fragments arranged by size:
1673 118 99
PstI C_TGCA'G
Cuts at: 0 1028 1043 1073 1385 1890
Size: 1028 15 30 312 505
Fragments arranged by size:
1028 505 312 30 15
PvuII CAG'CTG
Cuts at: 0 1035 1890
Size: 1035 855
RsrII CG'GwC_CG
Cuts at: 0 1016 1890
Size: 1016 874
SacI G_AGCT'C
Cuts at: 0 716 871 1256 1552 1890
Size: 716 155 385 296 338
Fragments arranged by size:
716 385 338 296 155
SacII CC_GC'GG
Cuts at: 0 384 1330 1370 1890
Size: 384 946 40 520
Fragments arranged by size:
946 520 384 40
SapI GCTCTTCn'nnn_
Cuts at: 0 280 1004 1772 1890
Size: 280 724 768 118
Fragments arranged by size:
768 724 280 118
SbfI CC_TGCA'GG
Cuts at: 0 1073 1385 1890
Size: 1073 312 505
Fragments arranged by size:
1073 505 312
SfcI C'TryA_G
Cuts at: 0 222 1024 1039 1069 1381 1890
Size: 222 802 15 30 312 509
Fragments arranged by size:
802 509 312 222 30 15
SfoI GGC'GCC
Cuts at: 0 322 654 1890
Size: 322 332 1236
Fragments arranged by size:
1236 332 322
SmaI CCC'GGG
Cuts at: 0 1657 1890
Size: 1657 233
SphI G_CATG'C
Cuts at: 0 109 1890
Size: 109 1781
SrfI GCCC'GGGC
Cuts at: 0 1657 1890
Size: 1657 233
StuI AGG'CCT
Cuts at: 0 1535 1798 1890
Size: 1535 263 92
Fragments arranged by size:
1535 263 92
StyI C'CwwG_G
Cuts at: 0 557 1799 1890
Size: 557 1242 91
Fragments arranged by size:
1242 557 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 532 1536 1890
Size: 532 1004 354
Fragments arranged by size:
1004 532 354
TatI w'GTAC_w
Cuts at: 0 1400 1890
Size: 1400 490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 94 126 463 495 1087 1119 1890
Size: 94 32 337 32 592 32 771
Fragments arranged by size:
771 592 337 94 32 32 32
XmaI C'CCGG_G
Cuts at: 0 1655 1890
Size: 1655 235
ZraI GAC'GTC
Cuts at: 0 1824 1890
Size: 1824 66
Enzymes that do cut:
AatII AccI AcuI AgeI AlfI AlwNI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MmeI MspA1I NarI NcoI NspI PasI PfoI PmlI
PpuMI PshAI PstI PvuII RsrII SacI SacII SapI
SbfI SfcI SfoI SmaI SphI SrfI StuI StyI
TaqII TatI TstI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BglI BglII BmtI
BplI BpuEI BsiWI BsmI BspEI BspHI BspMI BssHII
BstBI BstEII BstXI BstZ17I ClaI DraI DraIII EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MscI MslI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PpiI PsiI PspOMI PspXI PsrI PvuI SalI SanDI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SspI SwaI Tth111I XbaI XcmI XhoI XmnI