(Circular) (Six-base) MAPSORT of: pFN21AB8928.seq Check: 1331 from: 1 to: 5545 pFN21AB8928 5545 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2087 2087 Size: 5545 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 278 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2202 2248 2888 1937 Size: 265 46 640 4594 Fragments arranged by size: 4594 640 265 46 Acc65I G'GTAC_C Cuts at: 2866 2866 Size: 5545 AclI AA'CG_TT Cuts at: 1563 2180 3672 4045 1563 Size: 617 1492 373 3063 Fragments arranged by size: 3063 1492 617 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3608 4992 5260 1632 Size: 1976 1384 268 1917 Fragments arranged by size: 1976 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5348 AflIII A'CryG_T Cuts at: 1203 2139 4444 5421 1203 Size: 936 2305 977 1327 Fragments arranged by size: 2305 1327 977 936 AgeI A'CCGG_T Cuts at: 4376 4376 Size: 5545 AhdI GACnn_n'nnGTC Cuts at: 4274 4274 Size: 5545 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5545 AlwNI CAG_nnn'CTG Cuts at: 2029 3288 4860 2029 Size: 1259 1572 2714 Fragments arranged by size: 2714 1572 1259 ApaLI G'TGCA_C Cuts at: 3602 4758 3602 Size: 1156 4389 ApoI r'AATT_y Cuts at: 1493 2854 3080 3232 5341 1493 Size: 1361 226 152 2109 1697 Fragments arranged by size: 2109 1697 1361 226 152 AseI AT'TA_AT Cuts at: 160 1032 2277 4099 160 Size: 872 1245 1822 1606 Fragments arranged by size: 1822 1606 1245 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 AvaI C'yCGr_G Cuts at: 1870 1943 2870 1870 Size: 73 927 4545 Fragments arranged by size: 4545 927 73 BamHI G'GATC_C Cuts at: 2875 3242 2875 Size: 367 5178 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2866 4321 618 Size: 325 217 627 1079 1455 1842 Fragments arranged by size: 1842 1455 1079 627 325 217 BanII G_rGCy'C Cuts at: 1391 2864 1391 Size: 1473 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2461 2523 928 Size: 971 562 62 3950 Fragments arranged by size: 3950 971 562 62 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3736 3770 1903 Size: 34 1799 34 3678 Fragments arranged by size: 3678 1799 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4647 3437 Size: 1210 4335 BclI T'GATC_A Cuts at: 1548 2527 1548 Size: 979 4566 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4156 136 Size: 107 122 71 631 605 2484 1525 Fragments arranged by size: 2484 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2444 5540 2444 Size: 3096 2449 BlpI GC'TnA_GC Cuts at: 2962 2962 Size: 5545 Bme1580I G_kGCm'C Cuts at: 1790 3606 4762 1790 Size: 1816 1156 2573 Fragments arranged by size: 2573 1816 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3276 4234 442 Size: 416 53 83 399 24 1859 958 1753 Fragments arranged by size: 1859 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5545 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 5545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 4205 1239 Size: 1007 1959 2579 Fragments arranged by size: 2579 1959 1007 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2120 3670 4535 4833 2120 Size: 1550 865 298 2832 Fragments arranged by size: 2832 1550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4208 882 Size: 617 2709 2219 Fragments arranged by size: 2709 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2086 3336 493 Size: 711 882 1250 2702 Fragments arranged by size: 2702 1250 882 711 BsaBI GATnn'nnATC Cuts at: 5539 5539 Size: 5545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3352 3734 4369 275 Size: 53 83 186 936 1819 382 635 1451 Fragments arranged by size: 1819 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3978 4376 4650 4797 5208 3978 Size: 398 274 147 411 4315 Fragments arranged by size: 4315 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5320 Fragments arranged by size: 5320 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5545 BseYI C'CCAG_C Cuts at: 4748 5244 4748 Size: 496 5049 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 2797 1163 Size: 573 372 689 3911 Fragments arranged by size: 3911 689 573 372 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2995 3756 4784 1540 Size: 275 186 994 761 1028 2301 Fragments arranged by size: 2301 1028 994 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2272 3606 3691 4762 2272 Size: 1334 85 1071 3055 Fragments arranged by size: 3055 1334 1071 85 BsmI GAATG_Cn' Cuts at: 3056 3149 3056 Size: 93 5452 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2272 2864 3606 3691 4762 1391 Size: 399 482 592 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 592 482 399 85 BspEI T'CCGG_A Cuts at: 5208 5208 Size: 5545 BspHI T'CATG_A Cuts at: 3434 5164 3434 Size: 1730 3815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2087 2900 844 Size: 1243 813 3489 Fragments arranged by size: 3489 1243 813 BsrBI CCG'CTC Cuts at: 1394 1823 2062 3432 1394 Size: 429 239 1370 3507 Fragments arranged by size: 3507 1370 429 239 BsrDI GCAATG_nn' Cuts at: 65 1248 4040 4214 65 Size: 1183 2792 174 1396 Fragments arranged by size: 2792 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 4189 4376 1700 Size: 110 257 2122 187 2869 Fragments arranged by size: 2869 2122 257 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5545 BssSI C'ACGA_G Cuts at: 3605 4617 3605 Size: 1012 4533 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5545 BstBI TT'CG_AA Cuts at: 4349 4349 Size: 5545 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5545 BstXI CCAn_nnnn'nTGG Cuts at: 2712 2712 Size: 5545 BstYI r'GATC_y Cuts at: 1972 2383 2444 2626 2875 3242 3627 3644 5096 Size: 411 61 182 249 367 385 17 1452 Cuts at: 5096 5182 5194 5540 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 411 385 367 346 249 182 86 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4993 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4806 Fragments arranged by size: 4806 683 56 BtsI GCAGTG_nn' Cuts at: 3061 3855 3875 3061 Size: 794 20 4731 Fragments arranged by size: 4731 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5510 DraI TTT'AAA Cuts at: 2232 2849 2232 Size: 617 4928 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4552 809 Size: 507 3236 1802 Fragments arranged by size: 3236 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2992 3881 5510 Size: 54 1425 50 275 18 1162 889 1629 Cuts at: 5510 8 Size: 43 Fragments arranged by size: 1629 1425 1162 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2992 1537 Size: 275 1180 4090 Fragments arranged by size: 4090 1180 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3475 1353 Size: 2122 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4132 4506 4652 387 Size: 1064 138 2543 374 146 1280 Fragments arranged by size: 2543 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 3608 4205 4992 5260 1239 Size: 393 614 1362 597 787 268 1524 Fragments arranged by size: 1524 1362 787 614 597 393 268 EcoRI G'AATT_C Cuts at: 2854 3232 2854 Size: 378 5167 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2619 2619 Size: 5545 FspI TGC'GCA Cuts at: 4051 4051 Size: 5545 HaeII r_GCGC'y Cuts at: 1678 4692 1678 Size: 3014 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2822 2854 4186 4218 4260 Size: 32 135 32 1250 32 1332 32 42 Cuts at: 4260 4292 1373 Size: 32 2626 Fragments arranged by size: 2626 1332 1250 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2889 3135 669 Size: 988 281 951 246 3079 Fragments arranged by size: 3079 988 951 281 246 HindIII A'AGCT_T Cuts at: 748 2601 748 Size: 1853 3692 HpaI GTT'AAC Cuts at: 3135 3135 Size: 5545 KpnI G_GTAC'C Cuts at: 2870 2870 Size: 5545 MfeI C'AATT_G Cuts at: 2706 3144 2706 Size: 438 5107 MluI A'CGCG_T Cuts at: 5421 5421 Size: 5545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4659 4843 1305 Size: 3354 184 2007 Fragments arranged by size: 3354 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3723 Fragments arranged by size: 3723 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3503 3862 4021 518 Size: 648 2337 359 159 2042 Fragments arranged by size: 2337 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2470 2961 3638 4786 5031 1606 Size: 864 491 677 1148 245 2120 Fragments arranged by size: 2120 1148 864 677 491 245 NaeI GCC'GGC Cuts at: 2069 2069 Size: 5545 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4993 NdeI CA'TA_TG Cuts at: 387 387 Size: 5545 NgoMIV G'CCGG_C Cuts at: 2067 2067 Size: 5545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5545 NotI GC'GGCC_GC Cuts at: 2992 2992 Size: 5545 NspI r_CATG'y Cuts at: 1132 1229 2131 2903 4448 1132 Size: 97 902 772 1545 2229 Fragments arranged by size: 2229 1545 902 772 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5545 PciI A'CATG_T Cuts at: 4444 4444 Size: 5545 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5545 PmeI GTTT'AAAC Cuts at: 2849 2849 Size: 5545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 3646 5127 5159 3614 Size: 32 1481 32 4000 Fragments arranged by size: 4000 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5545 PsiI TTA'TAA Cuts at: 3115 3115 Size: 5545 PstI C_TGCA'G Cuts at: 830 2897 830 Size: 2067 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5545 SalI G'TCGA_C Cuts at: 1936 2887 1936 Size: 951 4594 SbfI CC_TGCA'GG Cuts at: 2897 2897 Size: 5545 ScaI AGT'ACT Cuts at: 2812 3793 5505 2812 Size: 981 1712 2852 Fragments arranged by size: 2852 1712 981 SfcI C'TryA_G Cuts at: 826 1045 2249 2893 4028 4709 4900 826 Size: 219 1204 644 1135 681 191 1471 Fragments arranged by size: 1471 1204 1135 681 644 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5545 SmaI CCC'GGG Cuts at: 1872 2872 1872 Size: 1000 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2099 3649 4550 4812 820 Size: 197 926 156 1550 901 262 1553 Fragments arranged by size: 1553 1550 926 901 262 197 156 SnaBI TAC'GTA Cuts at: 493 3336 493 Size: 2843 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5545 SphI G_CATG'C Cuts at: 1132 2903 1132 Size: 1771 3774 SspI AAT'ATT Cuts at: 5 52 3469 5 Size: 47 3417 2081 Fragments arranged by size: 3417 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5545 StyI C'CwwG_G Cuts at: 513 1065 2580 2984 513 Size: 552 1515 404 3074 Fragments arranged by size: 3074 1515 552 404 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3572 3589 3742 3927 4372 325 Size: 1255 1992 17 153 185 445 1498 Fragments arranged by size: 1992 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 2810 3791 Size: 274 80 33 51 1443 54 779 981 Cuts at: 3791 5503 96 Size: 1712 138 Fragments arranged by size: 1712 1443 981 779 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2361 2393 5505 5537 2361 Size: 32 3112 32 2369 Fragments arranged by size: 3112 2369 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5545 XbaI T'CTAG_A Cuts at: 2881 2881 Size: 5545 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2666 2757 2666 Size: 91 5454 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5545 XmaI C'CCGG_G Cuts at: 1870 2870 1870 Size: 1000 4545 XmnI GAAnn'nnTTC Cuts at: 2715 3674 2715 Size: 959 4586 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 276 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI