(Circular) (Six-base) MAPSORT of: pFN21AF0190.seq Check: 7452 from: 1 to: 6265 pFN21AF0190 6265 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2660 2660 Size: 6265 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2200 3608 1937 Size: 263 1408 4594 Fragments arranged by size: 4594 1408 263 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2098 2285 2492 3144 3395 4328 5712 5980 Size: 466 187 207 652 251 933 1384 268 Cuts at: 5980 1632 Size: 1917 Fragments arranged by size: 1917 1384 933 652 466 268 251 207 187 AfeI AGC'GCT Cuts at: 2021 2021 Size: 6265 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 2299 2610 5164 6141 1203 Size: 1096 311 2554 977 1327 Fragments arranged by size: 2554 1327 1096 977 311 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 2278 3171 3418 4008 5580 2278 Size: 893 247 590 1572 2963 Fragments arranged by size: 2963 1572 893 590 247 ApaI G_GGCC'C Cuts at: 2016 2030 2803 2935 2016 Size: 14 773 132 5346 Fragments arranged by size: 5346 773 132 14 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 3290 3574 3800 3952 6061 1493 Size: 1797 284 226 152 2109 1697 Fragments arranged by size: 2109 1797 1697 284 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2922 3304 3590 1870 Size: 73 72 907 382 286 4545 Fragments arranged by size: 4545 907 382 286 73 72 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2705 2738 2705 Size: 33 6232 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3264 3359 3586 5041 618 Size: 325 217 627 1477 95 227 1455 1842 Fragments arranged by size: 1842 1477 1455 627 325 227 217 95 BanII G_rGCy'C Cuts at: 1391 2016 2030 2189 2803 2935 3121 3324 3584 Size: 625 14 159 614 132 186 203 260 Cuts at: 3584 1391 Size: 4072 Fragments arranged by size: 4072 625 614 260 203 186 159 132 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2759 928 Size: 971 860 4434 Fragments arranged by size: 4434 971 860 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2684 4876 136 Size: 107 122 71 631 605 1012 2192 1525 Fragments arranged by size: 2192 1525 1012 631 605 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 2016 2030 2803 2935 3081 3267 3362 4326 Size: 226 14 773 132 146 186 95 964 Cuts at: 4326 5482 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 964 773 226 186 146 132 95 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2692 3395 3996 Size: 416 53 83 399 24 1275 703 601 Cuts at: 3996 4954 442 Size: 958 1753 Fragments arranged by size: 1753 1275 958 703 601 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2043 2546 2684 3057 4925 1239 Size: 804 503 138 373 1868 2579 Fragments arranged by size: 2579 1868 804 503 373 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4390 5255 5553 4390 Size: 865 298 5102 Fragments arranged by size: 5102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2436 4056 493 Size: 711 1232 1620 2702 Fragments arranged by size: 2702 1620 1232 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2918 3015 4072 4454 Size: 53 83 186 936 1385 97 1057 382 Cuts at: 4454 5089 275 Size: 635 1451 Fragments arranged by size: 1451 1385 1057 936 635 382 186 97 83 53 BsaWI w'CCGG_w Cuts at: 3370 4698 5096 5370 5517 5928 3370 Size: 1328 398 274 147 411 3707 Fragments arranged by size: 3707 1328 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2056 2086 2299 2329 3326 Size: 30 165 30 653 30 213 30 997 Cuts at: 3326 3356 1178 Size: 30 4087 Fragments arranged by size: 4087 997 653 213 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2426 2999 3002 3011 3111 3133 3181 3331 Size: 1240 573 3 9 100 22 48 150 Cuts at: 3331 3461 3470 1186 Size: 130 9 3981 Fragments arranged by size: 3981 1240 573 150 130 100 48 22 9 9 3 BseYI C'CCAG_C Cuts at: 2022 2318 2846 2935 3171 3206 3429 3498 5468 Size: 296 528 89 236 35 223 69 1970 Cuts at: 5468 5964 2022 Size: 496 2323 Fragments arranged by size: 2323 1970 528 496 296 236 223 89 69 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5692 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2189 2400 3066 3324 3515 4326 4411 5482 2189 Size: 211 666 258 191 811 85 1071 2972 Fragments arranged by size: 2972 1071 811 666 258 211 191 85 BsmI GAATG_Cn' Cuts at: 2486 3776 3869 2486 Size: 1290 93 4882 Fragments arranged by size: 4882 1290 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2030 2189 2400 2803 2935 3066 Size: 399 226 14 159 211 403 132 131 Cuts at: 3066 3081 3121 3267 3324 3362 3515 3584 4326 Size: 15 40 146 57 38 153 69 742 Cuts at: 4326 4411 5482 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 226 211 159 153 146 132 131 85 69 57 40 38 15 14 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2660 3620 844 Size: 1816 960 3489 Fragments arranged by size: 3489 1816 960 BsrBI CCG'CTC Cuts at: 1394 1823 2057 4152 1394 Size: 429 234 2095 3507 Fragments arranged by size: 3507 2095 429 234 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2377 4909 5096 1700 Size: 110 567 2532 187 2869 Fragments arranged by size: 2869 2532 567 187 110 BsrGI T'GTAC_A Cuts at: 96 2280 96 Size: 2184 4081 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstXI CCAn_nnnn'nTGG Cuts at: 3184 3184 Size: 6265 BstYI r'GATC_y Cuts at: 1972 2415 2467 3595 3962 4347 4364 5816 5902 Size: 443 52 1128 367 385 17 1452 86 Cuts at: 5902 5914 6260 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1128 443 385 367 346 86 52 17 12 Bsu36I CC'TnA_GG Cuts at: 3444 3444 Size: 6265 BtgI C'CryG_G Cuts at: 513 1065 2004 2536 2795 513 Size: 552 939 532 259 3983 Fragments arranged by size: 3983 939 552 532 259 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2688 3501 487 Size: 683 56 1462 813 3251 Fragments arranged by size: 3251 1462 813 683 56 BtsI GCAGTG_nn' Cuts at: 2584 3781 4575 4595 2584 Size: 1197 794 20 4254 Fragments arranged by size: 4254 1197 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DraIII CAC_nnn'GTG Cuts at: 2830 2830 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2539 3558 3712 Size: 54 1425 50 275 18 709 1019 154 Cuts at: 3712 4601 6230 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1019 889 709 275 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2214 2976 3312 3414 3447 4195 1353 Size: 861 762 336 102 33 748 3423 Fragments arranged by size: 3423 861 762 748 336 102 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2187 3322 2187 Size: 1135 5130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2098 2285 2492 2546 2684 3057 Size: 393 411 55 187 207 54 138 373 Cuts at: 3057 3144 3395 4328 4925 5712 5980 1239 Size: 87 251 933 597 787 268 1524 Fragments arranged by size: 1524 933 787 597 411 393 373 268 251 207 187 138 87 55 54 EcoNI CCTnn'n_nnAGG Cuts at: 2048 2972 3199 2048 Size: 924 227 5114 Fragments arranged by size: 5114 924 227 EcoO109I rG'GnC_Cy Cuts at: 2012 2026 2027 2067 2275 2668 2800 2931 2932 Size: 14 1 40 208 393 132 131 1 Cuts at: 2932 2965 3210 3395 2012 Size: 33 245 185 4882 Fragments arranged by size: 4882 393 245 208 185 132 131 40 33 14 1 1 EcoRI G'AATT_C Cuts at: 3290 3574 3952 3290 Size: 284 378 5603 Fragments arranged by size: 5603 378 284 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2023 5412 1678 Size: 345 3389 2531 Fragments arranged by size: 3389 2531 345 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2056 2088 2299 2331 3323 Size: 32 135 32 484 32 211 32 992 Cuts at: 3323 3355 4906 4938 4980 5012 1373 Size: 32 1551 32 42 32 2626 Fragments arranged by size: 2626 1551 992 484 211 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 2299 6141 2299 Size: 3842 2423 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5379 5563 1305 Size: 4074 184 2007 Fragments arranged by size: 4074 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2541 3560 10 Size: 54 1425 343 709 1019 2715 Fragments arranged by size: 2715 1425 1019 709 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4223 4582 4741 518 Size: 648 3057 359 159 2042 Fragments arranged by size: 3057 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2443 2570 2782 3498 3681 4358 5506 5751 Size: 837 127 212 716 183 677 1148 245 Cuts at: 5751 1606 Size: 2120 Fragments arranged by size: 2120 1148 837 716 677 245 212 183 127 NaeI GCC'GGC Cuts at: 2379 2379 Size: 6265 NcoI C'CATG_G Cuts at: 513 1065 2004 2536 513 Size: 552 939 532 4242 Fragments arranged by size: 4242 939 552 532 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NgoMIV G'CCGG_C Cuts at: 2377 2377 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2458 3623 5168 1132 Size: 97 1229 1165 1545 2229 Fragments arranged by size: 2229 1545 1229 1165 97 PasI CC'CwG_GG Cuts at: 1348 2138 2334 3114 3389 1348 Size: 790 196 780 275 4224 Fragments arranged by size: 4224 790 780 275 196 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 2144 1108 Size: 1036 5229 PfoI T'CCnGG_A Cuts at: 3312 3312 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2668 2965 3210 3395 2275 Size: 393 297 245 185 5145 Fragments arranged by size: 5145 393 297 245 185 PshAI GACnn'nnGTC Cuts at: 1613 2857 1613 Size: 1244 5021 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 2012 2026 2799 2931 2012 Size: 14 773 132 5346 Fragments arranged by size: 5346 773 132 14 PspXI vC'TCGA_Gb Cuts at: 2922 2922 Size: 6265 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2273 2305 2273 Size: 32 6233 PstI C_TGCA'G Cuts at: 830 2052 3617 830 Size: 1222 1565 3478 Fragments arranged by size: 3478 1565 1222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2443 2570 3498 2443 Size: 127 928 5210 Fragments arranged by size: 5210 928 127 SacI G_AGCT'C Cuts at: 2189 3324 2189 Size: 1135 5130 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SanDI GG'GwC_CC Cuts at: 3395 3395 Size: 6265 SapI GCTCTTCn'nnn_ Cuts at: 3312 3312 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 2048 3613 4748 5429 5620 826 Size: 219 1003 1565 1135 681 191 1471 Fragments arranged by size: 1565 1471 1135 1003 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2922 4369 5270 5532 820 Size: 197 926 979 1447 901 262 1553 Fragments arranged by size: 1553 1447 979 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 2458 3623 1132 Size: 1326 1165 3774 Fragments arranged by size: 3774 1326 1165 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 2004 2235 2536 3244 3297 3704 513 Size: 552 939 231 301 708 53 407 3074 Fragments arranged by size: 3074 939 708 552 407 301 231 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2326 2417 2957 3530 3539 4292 4309 Size: 1255 746 91 540 573 9 753 17 Cuts at: 4309 4462 4647 5092 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 753 746 573 540 445 185 153 91 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2280 4511 6223 Size: 274 80 33 51 1443 303 2231 1712 Cuts at: 6223 96 Size: 138 Fragments arranged by size: 2231 1712 1443 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3033 3065 6225 6257 3033 Size: 32 3160 32 3041 Fragments arranged by size: 3160 3041 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2679 3121 3251 2679 Size: 442 130 5693 Fragments arranged by size: 5693 442 130 XhoI C'TCGA_G Cuts at: 1943 2922 1943 Size: 979 5286 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI RsrII SacII SexAI SfiI SfoI SrfI SwaI