(Circular) (Six-base) MAPSORT of: pFN21AB7792.seq Check: 9034 from: 1 to: 6262 pFN21AB7792 6262 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3605 1937 Size: 1668 4594 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4325 5709 5977 1632 Size: 2693 1384 268 1917 Fragments arranged by size: 2693 1917 1384 268 AfeI AGC'GCT Cuts at: 2669 2669 Size: 6262 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 2028 3483 5161 6138 1203 Size: 825 1455 1678 977 1327 Fragments arranged by size: 1678 1455 1327 977 825 AgeI A'CCGG_T Cuts at: 3507 5093 3507 Size: 1586 4676 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 2610 1166 Size: 1444 4818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6262 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 2421 Size: 32 6230 AlwNI CAG_nnn'CTG Cuts at: 4005 5577 4005 Size: 1572 4690 ApaI G_GGCC'C Cuts at: 2391 2391 Size: 6262 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 2846 3571 3797 3949 6058 1493 Size: 1353 725 226 152 2109 1697 Fragments arranged by size: 2109 1697 1353 725 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 2105 2125 2266 3007 3172 3587 1870 Size: 73 162 20 141 741 165 415 4545 Fragments arranged by size: 4545 741 415 165 162 141 73 20 BamHI G'GATC_C Cuts at: 3592 3959 3592 Size: 367 5895 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3074 3148 3583 5038 618 Size: 325 217 627 1287 74 435 1455 1842 Fragments arranged by size: 1842 1455 1287 627 435 325 217 74 BanII G_rGCy'C Cuts at: 1391 2094 2391 2834 3581 1391 Size: 703 297 443 747 4072 Fragments arranged by size: 4072 747 703 443 297 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2763 928 Size: 971 864 4427 Fragments arranged by size: 4427 971 864 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4453 4487 1903 Size: 34 2516 34 3678 Fragments arranged by size: 3678 2516 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 1548 Size: 6262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2703 4873 136 Size: 107 122 71 631 605 1031 2170 1525 Fragments arranged by size: 2170 1525 1031 631 605 122 107 71 BglII A'GATC_T Cuts at: 2783 6257 2783 Size: 3474 2788 BlpI GC'TnA_GC Cuts at: 3679 3679 Size: 6262 Bme1580I G_kGCm'C Cuts at: 1790 2391 2708 3077 3153 4323 5479 1790 Size: 601 317 369 76 1170 1156 2573 Fragments arranged by size: 2573 1170 1156 601 369 317 76 BmgBI CAC'GTC Cuts at: 1318 3427 1318 Size: 2109 4153 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2711 3993 4951 Size: 416 53 83 399 24 1294 1282 958 Cuts at: 4951 442 Size: 1753 Fragments arranged by size: 1753 1294 1282 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2131 2131 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 2445 4922 1239 Size: 819 387 2477 2579 Fragments arranged by size: 2579 2477 819 387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3272 4387 5252 5550 3272 Size: 1115 865 298 3984 Fragments arranged by size: 3984 1115 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3239 4925 882 Size: 617 1740 1686 2219 Fragments arranged by size: 2219 1740 1686 617 BsaAI yAC'GTr Cuts at: 493 1204 2932 4053 493 Size: 711 1728 1121 2702 Fragments arranged by size: 2702 1728 1121 711 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2562 3002 3167 4069 Size: 53 83 186 936 1029 440 165 902 Cuts at: 4069 4451 5086 275 Size: 382 635 1451 Fragments arranged by size: 1451 1029 936 902 635 440 382 186 165 83 53 BsaWI w'CCGG_w Cuts at: 3507 4695 5093 5367 5514 5925 3507 Size: 1188 398 274 147 411 3844 Fragments arranged by size: 3844 1188 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6037 Fragments arranged by size: 6037 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3233 3308 1186 Size: 2047 75 4140 Fragments arranged by size: 4140 2047 75 BseYI C'CCAG_C Cuts at: 5465 5961 5465 Size: 496 5766 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3017 3038 1163 Size: 573 1281 21 4387 Fragments arranged by size: 4387 1281 573 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3712 4473 5501 1540 Size: 275 186 1711 761 1028 2301 Fragments arranged by size: 2301 1711 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2400 2834 3300 3414 3546 4323 4408 5479 2400 Size: 434 466 114 132 777 85 1071 3183 Fragments arranged by size: 3183 1071 777 466 434 132 114 85 BsmI GAATG_Cn' Cuts at: 2259 2331 2503 3773 3866 2259 Size: 72 172 1270 93 4655 Fragments arranged by size: 4655 1270 172 93 72 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2391 2400 2708 2834 3077 3153 Size: 399 304 297 9 308 126 243 76 Cuts at: 3153 3300 3414 3546 3581 4323 4408 5479 1391 Size: 147 114 132 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 308 304 297 243 147 132 126 114 85 76 35 9 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 4151 5881 4151 Size: 1730 4532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2490 3129 3321 3617 844 Size: 1646 639 192 296 3489 Fragments arranged by size: 3489 1646 639 296 192 BsrBI CCG'CTC Cuts at: 1394 1823 3064 4149 1394 Size: 429 1241 1085 3507 Fragments arranged by size: 3507 1241 1085 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4757 4931 65 Size: 1183 3509 174 1396 Fragments arranged by size: 3509 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3507 4906 5093 1700 Size: 110 1697 1399 187 2869 Fragments arranged by size: 2869 1697 1399 187 110 BsrGI T'GTAC_A Cuts at: 96 3387 3432 96 Size: 3291 45 2926 Fragments arranged by size: 3291 2926 45 BssHII G'CGCG_C Cuts at: 1925 3269 3319 1925 Size: 1344 50 4868 Fragments arranged by size: 4868 1344 50 BssSI C'ACGA_G Cuts at: 2789 4322 5334 2789 Size: 1533 1012 3717 Fragments arranged by size: 3717 1533 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2451 3183 3486 794 Size: 1657 732 303 3570 Fragments arranged by size: 3570 1657 732 303 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstXI CCAn_nnnn'nTGG Cuts at: 3445 3445 Size: 6262 BstYI r'GATC_y Cuts at: 1972 2783 3592 3959 4344 4361 5813 5899 5911 Size: 811 809 367 385 17 1452 86 12 Cuts at: 5911 6257 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 811 809 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2579 2579 Size: 6262 BtgI C'CryG_G Cuts at: 513 1065 2004 3111 3195 3454 513 Size: 552 939 1107 84 259 3321 Fragments arranged by size: 3321 1107 939 552 259 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2100 3241 487 Size: 683 56 874 1141 3508 Fragments arranged by size: 3508 1141 874 683 56 BtsI GCAGTG_nn' Cuts at: 3400 3778 4572 4592 3400 Size: 378 794 20 5070 Fragments arranged by size: 5070 794 378 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6227 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5269 809 Size: 507 3953 1802 Fragments arranged by size: 3953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2572 2623 Size: 54 1425 50 275 18 333 409 51 Cuts at: 2623 3709 4598 6227 8 Size: 1086 889 1629 43 Fragments arranged by size: 1629 1425 1086 889 409 333 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3709 1537 Size: 275 1897 4090 Fragments arranged by size: 4090 1897 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2193 3419 4192 1353 Size: 840 1226 773 3423 Fragments arranged by size: 3423 1226 840 773 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4849 5223 5369 387 Size: 1064 138 3260 374 146 1280 Fragments arranged by size: 3260 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2832 2832 Size: 6262 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2445 4325 4922 5709 5977 1239 Size: 393 426 387 1880 597 787 268 1524 Fragments arranged by size: 1880 1524 787 597 426 393 387 268 EcoO109I rG'GnC_Cy Cuts at: 2110 2457 2863 2110 Size: 347 406 5509 Fragments arranged by size: 5509 406 347 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 EcoRV GAT'ATC Cuts at: 2365 2365 Size: 6262 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 2671 2895 3111 5409 1678 Size: 993 224 216 2298 2531 Fragments arranged by size: 2531 2298 993 224 216 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3506 3538 4903 4935 4977 Size: 32 135 32 1934 32 1365 32 42 Cuts at: 4977 5009 1373 Size: 32 2626 Fragments arranged by size: 2626 1934 1365 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3852 669 Size: 988 281 1668 246 3079 Fragments arranged by size: 3079 1668 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6262 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2305 2895 3111 5376 5560 1305 Size: 1000 590 216 2265 184 2007 Fragments arranged by size: 2265 2007 1000 590 216 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2165 2574 10 Size: 54 1425 343 333 409 3698 Fragments arranged by size: 3698 1425 409 343 333 54 MslI CAynn'nnrTG Cuts at: 518 1166 2610 3443 4220 4579 4738 518 Size: 648 1444 833 777 359 159 2042 Fragments arranged by size: 2042 1444 833 777 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2992 3052 3231 3369 3678 4355 5503 5748 Size: 1386 60 179 138 309 677 1148 245 Cuts at: 5748 1606 Size: 2120 Fragments arranged by size: 2120 1386 1148 677 309 245 179 138 60 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4771 Fragments arranged by size: 4771 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 2032 3487 3620 5165 1132 Size: 97 803 1455 133 1545 2229 Fragments arranged by size: 2229 1545 1455 803 133 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 2028 3483 5161 2028 Size: 1455 1678 3129 Fragments arranged by size: 3129 1678 1455 PflMI CCAn_nnn'nTGG Cuts at: 1108 2935 1108 Size: 1827 4435 PfoI T'CCnGG_A Cuts at: 3417 3417 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PmlI CAC'GTG Cuts at: 2932 2932 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4331 4363 5844 5876 4331 Size: 32 1481 32 4717 Fragments arranged by size: 4717 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2110 2110 Size: 6262 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6262 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PspOMI G'GGCC_C Cuts at: 2387 2387 Size: 6262 PstI C_TGCA'G Cuts at: 830 3614 830 Size: 2784 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 PvuII CAG'CTG Cuts at: 3052 3052 Size: 6262 SacI G_AGCT'C Cuts at: 2834 2834 Size: 6262 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SapI GCTCTTCn'nnn_ Cuts at: 3419 3419 Size: 6262 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SfcI C'TryA_G Cuts at: 826 1045 3610 4745 5426 5617 826 Size: 219 2565 1135 681 191 1471 Fragments arranged by size: 2565 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 3009 3589 1872 Size: 1137 580 4545 Fragments arranged by size: 4545 1137 580 SmlI C'TyrA_G Cuts at: 820 1017 1943 3287 4366 5267 5529 820 Size: 197 926 1344 1079 901 262 1553 Fragments arranged by size: 1553 1344 1079 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6262 StyI C'CwwG_G Cuts at: 513 1065 2004 2299 3135 3701 513 Size: 552 939 295 836 566 3074 Fragments arranged by size: 3074 939 836 566 552 295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2483 4289 4306 4459 4644 5089 325 Size: 1255 903 1806 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 903 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3387 3432 4508 Size: 274 80 33 51 1443 1410 45 1076 Cuts at: 4508 6220 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1410 1076 274 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6222 6254 6222 Size: 32 6230 Tth111I GACn'n_nGTC Cuts at: 1316 2156 1316 Size: 840 5422 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2398 2965 3142 2398 Size: 567 177 5518 Fragments arranged by size: 5518 567 177 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 3007 3587 1870 Size: 1137 580 4545 Fragments arranged by size: 4545 1137 580 XmnI GAAnn'nnTTC Cuts at: 2519 4391 2519 Size: 1872 4390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI