(Circular) (Six-base) MAPSORT of: pFN21AB6480.seq Check: 6032 from: 1 to: 5716 pFN21AB6480 5716 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2952 3526 278 Size: 53 83 186 936 1416 574 2468 Fragments arranged by size: 2468 1416 936 574 186 83 53 AccI GT'mk_AC Cuts at: 1937 2190 3059 1937 Size: 253 869 4594 Fragments arranged by size: 4594 869 253 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2723 3779 5163 5431 1632 Size: 1091 1056 1384 268 1917 Fragments arranged by size: 1917 1384 1091 1056 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 4615 5592 1203 Size: 3412 977 1327 Fragments arranged by size: 3412 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 2242 2962 4445 2242 Size: 720 1483 3513 Fragments arranged by size: 3513 1483 720 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 2143 3459 5031 2143 Size: 1316 1572 2828 Fragments arranged by size: 2828 1572 1316 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 3025 3251 3403 5512 1493 Size: 1532 226 152 2109 1697 Fragments arranged by size: 2109 1697 1532 226 152 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 3041 1870 Size: 73 1098 4545 Fragments arranged by size: 4545 1098 73 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3037 4492 618 Size: 325 217 627 1250 1455 1842 Fragments arranged by size: 1842 1455 1250 627 325 217 BanII G_rGCy'C Cuts at: 1391 3035 1391 Size: 1644 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2041 928 Size: 971 142 4603 Fragments arranged by size: 4603 971 142 BbvCI CC'TCA_GC Cuts at: 2103 2308 2103 Size: 205 5511 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3907 3941 1903 Size: 34 50 34 1886 34 3678 Fragments arranged by size: 3678 1886 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 4327 136 Size: 107 122 71 631 605 394 2261 1525 Fragments arranged by size: 2261 1525 631 605 394 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 2355 3133 2355 Size: 778 4938 Bme1580I G_kGCm'C Cuts at: 1790 3777 4933 1790 Size: 1987 1156 2573 Fragments arranged by size: 2573 1987 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2551 3447 4405 Size: 416 53 83 399 24 1134 896 958 Cuts at: 4405 442 Size: 1753 Fragments arranged by size: 1753 1134 958 896 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2415 2602 4376 1239 Size: 1176 187 1774 2579 Fragments arranged by size: 2579 1774 1176 187 Bpu10I CC'TnA_GC Cuts at: 2103 2165 2308 2103 Size: 62 143 5511 Fragments arranged by size: 5511 143 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3841 4706 5004 3841 Size: 865 298 4553 Fragments arranged by size: 4553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 882 Size: 617 2880 2219 Fragments arranged by size: 2880 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3507 493 Size: 711 2303 2702 Fragments arranged by size: 2702 2303 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2949 3523 3905 4540 Size: 53 83 186 936 1416 574 382 635 Cuts at: 4540 275 Size: 1451 Fragments arranged by size: 1451 1416 936 635 574 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2980 4149 4547 4821 4968 5379 2980 Size: 1169 398 274 147 411 3317 Fragments arranged by size: 3317 1169 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5491 Fragments arranged by size: 5491 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2091 2473 1186 Size: 905 382 4429 Fragments arranged by size: 4429 905 382 BseYI C'CCAG_C Cuts at: 2088 2359 4919 5415 2088 Size: 271 2560 496 2389 Fragments arranged by size: 2560 2389 496 271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 1163 Size: 573 483 4660 Fragments arranged by size: 4660 573 483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2012 2235 3166 3927 4955 1540 Size: 275 186 11 223 931 761 1028 2301 Fragments arranged by size: 2301 1028 931 761 275 223 186 11 BsiHKAI G_wGCw'C Cuts at: 2368 3777 3862 4933 2368 Size: 1409 85 1071 3151 Fragments arranged by size: 3151 1409 1071 85 BsmI GAATG_Cn' Cuts at: 3227 3320 3227 Size: 93 5623 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2368 3035 3777 3862 4933 1391 Size: 399 578 667 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 667 578 399 85 BspEI T'CCGG_A Cuts at: 2980 5379 2980 Size: 2399 3317 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2128 3071 844 Size: 1284 943 3489 Fragments arranged by size: 3489 1284 943 BsrBI CCG'CTC Cuts at: 1394 1823 2099 3603 1394 Size: 429 276 1504 3507 Fragments arranged by size: 3507 1504 429 276 BsrDI GCAATG_nn' Cuts at: 65 1248 4211 4385 65 Size: 1183 2963 174 1396 Fragments arranged by size: 2963 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4360 4547 1700 Size: 110 2550 187 2869 Fragments arranged by size: 2869 2550 187 110 BsrGI T'GTAC_A Cuts at: 96 2124 2261 96 Size: 2028 137 3551 Fragments arranged by size: 3551 2028 137 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 2176 3776 4788 2176 Size: 1600 1012 3104 Fragments arranged by size: 3104 1600 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 2446 1184 Size: 1262 4454 BstXI CCAn_nnnn'nTGG Cuts at: 2599 2599 Size: 5716 BstYI r'GATC_y Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711 Size: 1074 367 385 17 1452 86 12 346 Cuts at: 5711 1972 Size: 1977 Fragments arranged by size: 1977 1452 1074 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2064 2511 2760 513 Size: 552 999 447 249 3469 Fragments arranged by size: 3469 999 552 447 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2482 487 Size: 683 56 1256 3721 Fragments arranged by size: 3721 1256 683 56 BtsI GCAGTG_nn' Cuts at: 2292 3232 4026 4046 2292 Size: 940 794 20 3962 Fragments arranged by size: 3962 940 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 2206 2889 3020 2206 Size: 683 131 4902 Fragments arranged by size: 4902 683 131 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 3163 4052 Size: 54 1425 50 275 18 402 931 889 Cuts at: 4052 5681 8 Size: 1629 43 Fragments arranged by size: 1629 1425 931 889 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2232 3163 1537 Size: 275 420 931 4090 Fragments arranged by size: 4090 931 420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2874 3646 1353 Size: 1521 772 3423 Fragments arranged by size: 3423 1521 772 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4303 4677 4823 387 Size: 1064 138 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 2602 2723 3779 4376 5163 5431 Size: 393 783 187 121 1056 597 787 268 Cuts at: 5431 1239 Size: 1524 Fragments arranged by size: 1524 1056 787 783 597 393 268 187 121 EcoO109I rG'GnC_Cy Cuts at: 2140 2573 2140 Size: 433 5283 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 4863 1678 Size: 3185 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 2948 2980 4357 Size: 32 135 32 656 32 688 32 1377 Cuts at: 4357 4389 4431 4463 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1377 688 656 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3306 669 Size: 988 281 1122 246 3079 Fragments arranged by size: 3079 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2584 4830 5014 1305 Size: 1279 2246 184 2007 Fragments arranged by size: 2246 2007 1279 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2252 2444 3674 4033 4192 518 Size: 648 1086 192 1230 359 159 2042 Fragments arranged by size: 2042 1230 1086 648 359 192 159 MspA1I CmG'CkG Cuts at: 1606 2066 2092 3132 3809 4957 5202 1606 Size: 460 26 1040 677 1148 245 2120 Fragments arranged by size: 2120 1148 1040 677 460 245 26 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5164 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 3074 4619 1132 Size: 97 1845 1545 2229 Fragments arranged by size: 2229 1845 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5716 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5716 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2140 2140 Size: 5716 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PstI C_TGCA'G Cuts at: 830 3068 830 Size: 2238 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 PvuII CAG'CTG Cuts at: 2092 2092 Size: 5716 SacII CC_GC'GG Cuts at: 2067 2067 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SfcI C'TryA_G Cuts at: 826 1045 3064 4199 4880 5071 826 Size: 219 2019 1135 681 191 1471 Fragments arranged by size: 2019 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3820 4721 4983 820 Size: 197 926 1877 901 262 1553 Fragments arranged by size: 1877 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 2182 2829 2918 1517 Size: 665 647 89 4315 Fragments arranged by size: 4315 665 647 89 StyI C'CwwG_G Cuts at: 513 1065 2393 2919 3155 513 Size: 552 1328 526 236 3074 Fragments arranged by size: 3074 1328 552 526 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2371 3743 3760 3913 4098 4543 Size: 1255 446 345 1372 17 153 185 445 Cuts at: 4543 325 Size: 1498 Fragments arranged by size: 1498 1372 1255 446 445 345 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 2261 2625 Size: 274 80 33 51 1443 147 137 364 Cuts at: 2625 3962 5674 96 Size: 1337 1712 138 Fragments arranged by size: 1712 1443 1337 364 274 147 138 137 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2847 2879 5676 5708 2847 Size: 32 2797 32 2855 Fragments arranged by size: 2855 2797 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 2770 3000 3845 2770 Size: 230 845 4641 Fragments arranged by size: 4641 845 230 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2950 3524 276 Size: 53 83 186 936 1416 574 2468 Fragments arranged by size: 2468 1416 936 574 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI