(Circular) (Six-base) MAPSORT of: pFN21AB6732.seq Check: 4475 from: 1 to: 5095 pFN21AB6732 5095 bp With 182 enzymes: SgfI * October 20, 2009 10:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2905 278 Size: 53 83 186 936 1369 2468 Fragments arranged by size: 2468 1369 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2438 1937 Size: 501 4594 Acc65I G'GTAC_C Cuts at: 2416 2416 Size: 5095 AclI AA'CG_TT Cuts at: 1563 3222 3595 1563 Size: 1659 373 3063 Fragments arranged by size: 3063 1659 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3158 4542 4810 1632 Size: 1526 1384 268 1917 Fragments arranged by size: 1917 1526 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4898 AflIII A'CryG_T Cuts at: 1203 3994 4971 1203 Size: 2791 977 1327 Fragments arranged by size: 2791 1327 977 AgeI A'CCGG_T Cuts at: 3926 3926 Size: 5095 AhdI GACnn_n'nnGTC Cuts at: 2159 3824 2159 Size: 1665 3430 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5095 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5095 AlwNI CAG_nnn'CTG Cuts at: 2838 4410 2838 Size: 1572 3523 ApaLI G'TGCA_C Cuts at: 3152 4308 3152 Size: 1156 3939 ApoI r'AATT_y Cuts at: 1493 2404 2630 2782 4891 1493 Size: 911 226 152 2109 1697 Fragments arranged by size: 2109 1697 911 226 152 AseI AT'TA_AT Cuts at: 160 1032 3649 160 Size: 872 2617 1606 Fragments arranged by size: 2617 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5095 AvaI C'yCGr_G Cuts at: 1870 1943 2300 2420 1870 Size: 73 357 120 4545 Fragments arranged by size: 4545 357 120 73 BamHI G'GATC_C Cuts at: 2425 2792 2425 Size: 367 4728 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2192 2339 2416 3871 618 Size: 325 217 627 405 147 77 1455 1842 Fragments arranged by size: 1842 1455 627 405 325 217 147 77 BanII G_rGCy'C Cuts at: 1391 2099 2414 1391 Size: 708 315 4072 Fragments arranged by size: 4072 708 315 BbeI G_GCGC'C Cuts at: 2196 2196 Size: 5095 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2302 2336 3286 3320 1903 Size: 34 50 34 281 34 950 34 3678 Fragments arranged by size: 3678 950 281 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2987 4197 2987 Size: 1210 3885 BclI T'GATC_A Cuts at: 1548 1548 Size: 5095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3706 136 Size: 107 122 71 631 605 2034 1525 Fragments arranged by size: 2034 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5090 5090 Size: 5095 BlpI GC'TnA_GC Cuts at: 2512 2512 Size: 5095 Bme1580I G_kGCm'C Cuts at: 1790 2268 2344 3156 4312 1790 Size: 478 76 812 1156 2573 Fragments arranged by size: 2573 1156 812 478 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5095 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2826 3784 442 Size: 416 53 83 399 24 1409 958 1753 Fragments arranged by size: 1753 1409 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5095 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2171 2171 Size: 5095 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3755 1239 Size: 2516 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3220 4085 4383 3220 Size: 865 298 3932 Fragments arranged by size: 3932 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3758 882 Size: 617 2259 2219 Fragments arranged by size: 2259 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2886 493 Size: 711 1682 2702 Fragments arranged by size: 2702 1682 711 BsaBI GATnn'nnATC Cuts at: 5089 5089 Size: 5095 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2193 2902 3284 3919 Size: 53 83 186 936 660 709 382 635 Cuts at: 3919 275 Size: 1451 Fragments arranged by size: 1451 936 709 660 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3528 3926 4200 4347 4758 3528 Size: 398 274 147 411 3865 Fragments arranged by size: 3865 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2093 2123 1178 Size: 30 165 30 690 30 4150 Fragments arranged by size: 4150 690 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2163 2396 2405 1186 Size: 977 233 9 3876 Fragments arranged by size: 3876 977 233 9 BseYI C'CCAG_C Cuts at: 2179 4298 4794 2179 Size: 2119 496 2480 Fragments arranged by size: 2480 2119 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2545 3306 4334 1540 Size: 275 186 544 761 1028 2301 Fragments arranged by size: 2301 1028 761 544 275 186 BsiHKAI G_wGCw'C Cuts at: 2090 2099 3156 3241 4312 2090 Size: 9 1057 85 1071 2873 Fragments arranged by size: 2873 1071 1057 85 9 BsmI GAATG_Cn' Cuts at: 2606 2699 2606 Size: 93 5002 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2099 2268 2344 2414 3156 3241 Size: 399 300 9 169 76 70 742 85 Cuts at: 3241 4312 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 300 169 85 76 70 9 BspEI T'CCGG_A Cuts at: 4758 4758 Size: 5095 BspHI T'CATG_A Cuts at: 2984 4714 2984 Size: 1730 3365 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2049 2063 2450 844 Size: 1205 14 387 3489 Fragments arranged by size: 3489 1205 387 14 BsrBI CCG'CTC Cuts at: 1394 1823 2982 1394 Size: 429 1159 3507 Fragments arranged by size: 3507 1159 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3590 3764 65 Size: 1183 2342 174 1396 Fragments arranged by size: 2342 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2189 2195 3739 3926 1700 Size: 110 379 6 1544 187 2869 Fragments arranged by size: 2869 1544 379 187 110 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5095 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5095 BssSI C'ACGA_G Cuts at: 3155 4167 3155 Size: 1012 4083 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5095 BstBI TT'CG_AA Cuts at: 3899 3899 Size: 5095 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5095 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2215 Size: 5095 BstYI r'GATC_y Cuts at: 1972 2425 2792 3177 3194 4646 4732 4744 5090 Size: 453 367 385 17 1452 86 12 346 Cuts at: 5090 1972 Size: 1977 Fragments arranged by size: 1977 1452 453 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4543 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2185 487 Size: 683 56 959 3397 Fragments arranged by size: 3397 959 683 56 BtsI GCAGTG_nn' Cuts at: 2065 2611 3405 3425 2065 Size: 546 794 20 3735 Fragments arranged by size: 3735 794 546 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5095 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5060 DraI TTT'AAA Cuts at: 2399 2399 Size: 5095 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4102 809 Size: 507 2786 1802 Fragments arranged by size: 2786 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2205 2283 Size: 54 1425 50 275 18 309 66 78 Cuts at: 2283 2542 3431 5060 8 Size: 259 889 1629 43 Fragments arranged by size: 1629 1425 889 309 275 259 78 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2542 1537 Size: 275 730 4090 Fragments arranged by size: 4090 730 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 3025 1353 Size: 701 971 3423 Fragments arranged by size: 3423 971 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3682 4056 4202 387 Size: 1064 138 2093 374 146 1280 Fragments arranged by size: 2093 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2097 2097 Size: 5095 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3158 3755 4542 4810 1239 Size: 393 1526 597 787 268 1524 Fragments arranged by size: 1526 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2125 2125 Size: 5095 EcoRI G'AATT_C Cuts at: 2404 2782 2404 Size: 378 4717 FspI TGC'GCA Cuts at: 3601 3601 Size: 5095 HaeII r_GCGC'y Cuts at: 1678 2196 4242 1678 Size: 518 2046 2531 Fragments arranged by size: 2531 2046 518 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2145 2177 3736 Size: 32 135 32 521 32 20 32 1559 Cuts at: 3736 3768 3810 3842 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1559 521 135 42 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 2439 2685 669 Size: 988 281 501 246 3079 Fragments arranged by size: 3079 988 501 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5095 HpaI GTT'AAC Cuts at: 2685 2685 Size: 5095 KasI G'GCGC_C Cuts at: 2192 2192 Size: 5095 KpnI G_GTAC'C Cuts at: 2420 2420 Size: 5095 MfeI C'AATT_G Cuts at: 2694 2694 Size: 5095 MluI A'CGCG_T Cuts at: 4971 4971 Size: 5095 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2125 4209 4393 1305 Size: 820 2084 184 2007 Fragments arranged by size: 2084 2007 820 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2285 10 Size: 54 1425 343 453 2820 Fragments arranged by size: 2820 1425 453 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3053 3412 3571 518 Size: 648 1887 359 159 2042 Fragments arranged by size: 2042 1887 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2106 2511 3188 4336 4581 1606 Size: 500 405 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 500 405 245 NaeI GCC'GGC Cuts at: 2197 2197 Size: 5095 NarI GG'CG_CC Cuts at: 2193 2193 Size: 5095 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4543 NdeI CA'TA_TG Cuts at: 387 387 Size: 5095 NgoMIV G'CCGG_C Cuts at: 2195 2195 Size: 5095 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5095 NotI GC'GGCC_GC Cuts at: 2542 2542 Size: 5095 NspI r_CATG'y Cuts at: 1132 1229 2453 3998 1132 Size: 97 1224 1545 2229 Fragments arranged by size: 2229 1545 1224 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5095 PciI A'CATG_T Cuts at: 3994 3994 Size: 5095 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5095 PmeI GTTT'AAAC Cuts at: 2399 2399 Size: 5095 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3164 3196 4677 4709 3164 Size: 32 1481 32 3550 Fragments arranged by size: 3550 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5095 PsiI TTA'TAA Cuts at: 2665 2665 Size: 5095 PstI C_TGCA'G Cuts at: 830 2295 2447 830 Size: 1465 152 3478 Fragments arranged by size: 3478 1465 152 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5095 SacI G_AGCT'C Cuts at: 2099 2099 Size: 5095 SalI G'TCGA_C Cuts at: 1936 2437 1936 Size: 501 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 5095 SbfI CC_TGCA'GG Cuts at: 2447 2447 Size: 5095 ScaI AGT'ACT Cuts at: 3343 5055 3343 Size: 1712 3383 SfcI C'TryA_G Cuts at: 826 1045 2291 2443 3578 4259 4450 826 Size: 219 1246 152 1135 681 191 1471 Fragments arranged by size: 1471 1246 1135 681 219 191 152 SfoI GGC'GCC Cuts at: 2194 2194 Size: 5095 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5095 SgrAI Cr'CCGG_yG Cuts at: 1700 2189 1700 Size: 489 4606 SmaI CCC'GGG Cuts at: 1872 2422 1872 Size: 550 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3199 4100 4362 820 Size: 197 926 1256 901 262 1553 Fragments arranged by size: 1553 1256 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2886 493 Size: 2393 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5095 SphI G_CATG'C Cuts at: 1132 2453 1132 Size: 1321 3774 SspI AAT'ATT Cuts at: 5 52 2335 3019 5 Size: 47 2283 684 2081 Fragments arranged by size: 2283 2081 684 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5095 StyI C'CwwG_G Cuts at: 513 1065 2534 513 Size: 552 1469 3074 Fragments arranged by size: 3074 1469 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2375 3122 3139 3292 3477 3922 325 Size: 1255 795 747 17 153 185 445 1498 Fragments arranged by size: 1498 1255 795 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3341 5053 96 Size: 274 80 33 51 1443 1364 1712 138 Fragments arranged by size: 1712 1443 1364 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5055 5087 5055 Size: 32 5063 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5095 XbaI T'CTAG_A Cuts at: 2431 2431 Size: 5095 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5095 XmaI C'CCGG_G Cuts at: 1870 2420 1870 Size: 550 4545 XmnI GAAnn'nnTTC Cuts at: 3224 3224 Size: 5095 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2903 276 Size: 53 83 186 936 1369 2468 Fragments arranged by size: 2468 1369 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI