Restriction map for
pFN21AB6330
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I CfoI ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoRI EcoT22I
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MamI MfeI MluI
MluNI MroI MscI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 751 GACGTC GACGT^C
AccII 784 CGCG CG^CG
AcyI 751 GACGTC G[GA]^CG[CT]C
AocI 435 CCTCAGG CC^T[ACGT]AGG
AspI 115 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 97 CTCGGG C^[CT]CG[GA]G
BamHI 212 GGATCC G^GATCC
BbsI 844 GTCTTC GAAGAC(2/6)
BclI 245 TGATCA T^GATCA
BglI 584 GCCAAATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 473 AGATCT A^GATCT
BpmI 757 CTGGAG CTGGAG(16/14)
BpuAI 844 GTCTTC GAAGAC(2/6)
BsaHI 751 GACGTC G[GA]^CG[CT]C
BsgI 331 GTGCAG GTGCAG(16/14)
BsmAI 637 GTCTC GTCTC(1/5)
BsoBI 97 CTCGGG C^[CT]CG[GA]G
BsrBI 696 CCGCTC CCGCTC(-3/-3)
BsrFI 320 GCCGGC [GA]^CCGG[CT]
BstAPI 276 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 360 GGTCACC G^GT[ACGT]ACC
BstPI 360 GGTCACC G^GT[ACGT]ACC
BstUI 784 CGCG CG^CG
BstXI 390 CCAGTTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 435 CCTCAGG CC^T[ACGT]AGG
Cfr10I 320 GCCGGC [GA]^CCGG[CT]
DraIII 608 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 435 CCTCAGG CC^T[ACGT]AGG
EcoNI 33 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 360 GGTCACC G^GT[ACGT]ACC
EcoRV 724 GATATC GAT^ATC
FbaI 245 TGATCA T^GATCA
HgaI 576 GCGTC GACGC(5/10)
Hin1I 751 GACGTC G[GA]^CG[CT]C
KspI 783 CCGCGG CCGC^GG
MslI 369 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 784 CGCG CG^CG
NaeI 320 GCCGGC GCC^GGC
NcoI 356 CCATGG C^CATGG
NgoMIV 320 GCCGGC G^CCGGC
PflFI 115 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 76 CTGCAG CTGCA^G
PvuII 82 CAGCTG CAG^CTG
SacI 94 GAGCTC GAGCT^C
SacII 783 CCGCGG CCGC^GG
SfaNI 16 GATGC GCATC(5/9)
SfcI 76 CTGCAG C^T[GA][CT]AG
SmlI 55 CTCAAG C^T[CT][GA]AG
Tsp509I 205 AATT ^AATT
Tth111I 115 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 703 GTAC GT^AC
817 GTAC
BanII 94 GAGCTC G[GA]GC[CT]^C
234 GGGCTC
BcnI 177 CCCGG CC^[GC]GG
738 CCCGG
BlpI 271 GCTGAGC GC^T[ACGT]AGC
571 GCTCAGC
Bpu1102I 271 GCTGAGC GC^T[ACGT]AGC
571 GCTCAGC
CelII 271 GCTGAGC GC^T[ACGT]AGC
571 GCTCAGC
DsaI 356 CCATGG C^C[GA][CT]GG
783 CCGCGG
EaeI 399 TGGCCG [CT]^GGCC[GA]
591 CGGCCA
MaeII 515 ACGT A^CGT
752 ACGT
MaeIII 361 GTCAC ^GT[ACGT]AC
415 GTCAC
MboII 476 TCTTC GAAGA(8/7)
845 TCTTC
MspA1I 82 CAGCTG C[AC]G^C[GT]G
783 CCGCGG
NciI 177 CCCGG CC^[GC]GG
738 CCCGG
RsaI 703 GTAC GT^AC
817 GTAC
Tsp45I 361 GTCAC ^GT[GC]AC
415 GTCAC
XcmI 137 CCAAGATCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
368 CCATCCAGGTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BbvI BfaI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MboI MflI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PpuMI Sau3AI Sau96I ScrFI
StyI TseI TspRI Van91I XhoII