(Circular) (Six-base) MAPSORT of: pFN21AB6330.seq Check: 5471 from: 1 to: 5569 pFN21AB6330 5569 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2272 2272 Size: 5569 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2760 3379 278 Size: 53 83 186 936 1224 619 2468 Fragments arranged by size: 2468 1224 936 619 186 83 53 AccI GT'mk_AC Cuts at: 1937 2912 1937 Size: 975 4594 Acc65I G'GTAC_C Cuts at: 2890 2890 Size: 5569 AclI AA'CG_TT Cuts at: 1563 3696 4069 1563 Size: 2133 373 3063 Fragments arranged by size: 3063 2133 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2024 3632 5016 5284 1632 Size: 392 1608 1384 268 1917 Fragments arranged by size: 1917 1608 1384 392 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5372 AflIII A'CryG_T Cuts at: 1203 4468 5445 1203 Size: 3265 977 1327 Fragments arranged by size: 3265 1327 977 AgeI A'CCGG_T Cuts at: 4400 4400 Size: 5569 AhdI GACnn_n'nnGTC Cuts at: 4298 4298 Size: 5569 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5569 AlwNI CAG_nnn'CTG Cuts at: 2089 2287 2584 3312 4884 2089 Size: 198 297 728 1572 2774 Fragments arranged by size: 2774 1572 728 297 198 ApaLI G'TGCA_C Cuts at: 3626 4782 3626 Size: 1156 4413 ApoI r'AATT_y Cuts at: 1493 2878 3104 3256 5365 1493 Size: 1385 226 152 2109 1697 Fragments arranged by size: 2109 1697 1385 226 152 AseI AT'TA_AT Cuts at: 160 1032 4123 160 Size: 872 3091 1606 Fragments arranged by size: 3091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 AvaI C'yCGr_G Cuts at: 1870 1943 2102 2894 1870 Size: 73 159 792 4545 Fragments arranged by size: 4545 792 159 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2805 2838 2805 Size: 33 5536 BamHI G'GATC_C Cuts at: 2217 2899 3266 2217 Size: 682 367 4520 Fragments arranged by size: 4520 682 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2890 4345 618 Size: 325 217 627 1103 1455 1842 Fragments arranged by size: 1842 1455 1103 627 325 217 BanII G_rGCy'C Cuts at: 1391 2103 2243 2888 1391 Size: 712 140 645 4072 Fragments arranged by size: 4072 712 645 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2842 928 Size: 971 943 3655 Fragments arranged by size: 3655 971 943 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3760 3794 1903 Size: 34 1823 34 3678 Fragments arranged by size: 3678 1823 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3461 4671 3461 Size: 1210 4359 BclI T'GATC_A Cuts at: 1548 2250 1548 Size: 702 4867 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2595 4180 136 Size: 107 122 71 631 605 923 1585 1525 Fragments arranged by size: 1585 1525 923 631 605 122 107 71 BglII A'GATC_T Cuts at: 2478 5564 2478 Size: 3086 2483 BlpI GC'TnA_GC Cuts at: 2277 2577 2986 2277 Size: 300 409 4860 Fragments arranged by size: 4860 409 300 Bme1580I G_kGCm'C Cuts at: 1790 2142 3630 4786 1790 Size: 352 1488 1156 2573 Fragments arranged by size: 2573 1488 1156 352 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2624 3300 4258 Size: 416 53 83 399 24 1207 676 958 Cuts at: 4258 442 Size: 1753 Fragments arranged by size: 1753 1207 958 676 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5569 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2661 2661 Size: 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2783 4229 1239 Size: 1544 1446 2579 Fragments arranged by size: 2579 1544 1446 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2045 3694 4559 4857 2045 Size: 1649 865 298 2757 Fragments arranged by size: 2757 1649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4232 882 Size: 617 2733 2219 Fragments arranged by size: 2733 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3360 493 Size: 711 2156 2702 Fragments arranged by size: 2702 2156 711 BsaBI GATnn'nnATC Cuts at: 5563 5563 Size: 5569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2757 3376 3758 4393 Size: 53 83 186 936 1224 619 382 635 Cuts at: 4393 275 Size: 1451 Fragments arranged by size: 1451 1224 936 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4002 4400 4674 4821 5232 4002 Size: 398 274 147 411 4339 Fragments arranged by size: 4339 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5344 Fragments arranged by size: 5344 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2333 2468 2471 1186 Size: 874 273 135 3 4284 Fragments arranged by size: 4284 874 273 135 3 BseYI C'CCAG_C Cuts at: 2172 2551 4772 5268 2172 Size: 379 2221 496 2473 Fragments arranged by size: 2473 2221 496 379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 1163 Size: 573 621 4375 Fragments arranged by size: 4375 621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3019 3780 4808 1540 Size: 275 186 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2103 2304 2718 3630 3715 4786 2103 Size: 201 414 912 85 1071 2886 Fragments arranged by size: 2886 1071 912 414 201 85 BsmI GAATG_Cn' Cuts at: 3080 3173 3080 Size: 93 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2142 2243 2304 2718 2888 3630 Size: 399 313 39 101 61 414 170 742 Cuts at: 3630 3715 4786 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 414 399 313 170 101 85 61 39 BspEI T'CCGG_A Cuts at: 5232 5232 Size: 5569 BspHI T'CATG_A Cuts at: 3458 5188 3458 Size: 1730 3839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 2272 2329 2924 844 Size: 1244 184 57 595 3489 Fragments arranged by size: 3489 1244 595 184 57 BsrBI CCG'CTC Cuts at: 1394 1823 2703 3456 1394 Size: 429 880 753 3507 Fragments arranged by size: 3507 880 753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4064 4238 65 Size: 1183 2816 174 1396 Fragments arranged by size: 2816 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2325 4213 4400 1700 Size: 110 515 1888 187 2869 Fragments arranged by size: 2869 1888 515 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5569 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5569 BssSI C'ACGA_G Cuts at: 3629 4641 3629 Size: 1012 4557 BstAPI GCAn_nnn'nTGC Cuts at: 794 2287 794 Size: 1493 4076 BstBI TT'CG_AA Cuts at: 4373 4373 Size: 5569 BstEII G'GTnAC_C Cuts at: 1184 2365 1184 Size: 1181 4388 BstXI CCAn_nnnn'nTGG Cuts at: 2402 2402 Size: 5569 BstYI r'GATC_y Cuts at: 1972 2145 2217 2478 2664 2766 2899 3266 3651 Size: 173 72 261 186 102 133 367 385 Cuts at: 3651 3668 5120 5206 5218 5564 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 261 186 173 133 102 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 2441 2441 Size: 5569 BtgI C'CryG_G Cuts at: 513 1065 2361 2788 513 Size: 552 1296 427 3294 Fragments arranged by size: 3294 1296 552 427 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2526 487 Size: 683 56 1300 3530 Fragments arranged by size: 3530 1300 683 56 BtsI GCAGTG_nn' Cuts at: 2221 3085 3879 3899 2221 Size: 864 794 20 3891 Fragments arranged by size: 3891 864 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5534 DraI TTT'AAA Cuts at: 2873 2873 Size: 5569 DraIII CAC_nnn'GTG Cuts at: 2618 2618 Size: 5569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4576 809 Size: 507 3260 1802 Fragments arranged by size: 3260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2404 2596 3016 Size: 54 1425 50 275 18 574 192 420 Cuts at: 3016 3905 5534 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 574 420 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3016 1537 Size: 275 1204 4090 Fragments arranged by size: 4090 1204 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3499 1353 Size: 2146 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4156 4530 4676 387 Size: 1064 138 2567 374 146 1280 Fragments arranged by size: 2567 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2101 2101 Size: 5569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2024 2783 3632 4229 5016 5284 1239 Size: 393 392 759 849 597 787 268 1524 Fragments arranged by size: 1524 849 787 759 597 393 392 268 EcoNI CCTnn'n_nnAGG Cuts at: 2042 2042 Size: 5569 EcoO109I rG'GnC_Cy Cuts at: 2077 2125 2179 2809 2839 2077 Size: 48 54 630 30 4807 Fragments arranged by size: 4807 630 54 48 30 EcoRI G'AATT_C Cuts at: 2878 3256 2878 Size: 378 5191 EcoRV GAT'ATC Cuts at: 2731 2731 Size: 5569 FspI TGC'GCA Cuts at: 4075 4075 Size: 5569 HaeII r_GCGC'y Cuts at: 1678 4716 1678 Size: 3038 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2669 2701 4210 4242 4284 Size: 32 135 32 1097 32 1509 32 42 Cuts at: 4284 4316 1373 Size: 32 2626 Fragments arranged by size: 2626 1509 1097 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2913 3159 669 Size: 988 281 975 246 3079 Fragments arranged by size: 3079 988 975 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5569 HpaI GTT'AAC Cuts at: 3159 3159 Size: 5569 KpnI G_GTAC'C Cuts at: 2894 2894 Size: 5569 MfeI C'AATT_G Cuts at: 3168 3168 Size: 5569 MluI A'CGCG_T Cuts at: 5445 5445 Size: 5569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4683 4867 1305 Size: 3378 184 2007 Fragments arranged by size: 3378 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3747 Fragments arranged by size: 3747 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2378 3527 3886 4045 518 Size: 648 1212 1149 359 159 2042 Fragments arranged by size: 2042 1212 1149 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2790 2985 3662 4810 5055 1606 Size: 483 701 195 677 1148 245 2120 Fragments arranged by size: 2120 1148 701 677 483 245 195 NaeI GCC'GGC Cuts at: 2327 2327 Size: 5569 NcoI C'CATG_G Cuts at: 513 1065 2361 513 Size: 552 1296 3721 Fragments arranged by size: 3721 1296 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5569 NgoMIV G'CCGG_C Cuts at: 2325 2325 Size: 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5569 NotI GC'GGCC_GC Cuts at: 3016 3016 Size: 5569 NspI r_CATG'y Cuts at: 1132 1229 2927 4472 1132 Size: 97 1698 1545 2229 Fragments arranged by size: 2229 1698 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5569 PciI A'CATG_T Cuts at: 4468 4468 Size: 5569 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 2383 2618 1108 Size: 1242 33 235 4059 Fragments arranged by size: 4059 1242 235 33 PfoI T'CCnGG_A Cuts at: 2760 2760 Size: 5569 PmeI GTTT'AAAC Cuts at: 2873 2873 Size: 5569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3670 5151 5183 3638 Size: 32 1481 32 4024 Fragments arranged by size: 4024 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2077 2125 2179 2077 Size: 48 54 5467 Fragments arranged by size: 5467 54 48 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5569 PsiI TTA'TAA Cuts at: 3139 3139 Size: 5569 PstI C_TGCA'G Cuts at: 830 2085 2921 830 Size: 1255 836 3478 Fragments arranged by size: 3478 1255 836 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5569 PvuII CAG'CTG Cuts at: 2089 2089 Size: 5569 SacI G_AGCT'C Cuts at: 2103 2103 Size: 5569 SacII CC_GC'GG Cuts at: 2791 2791 Size: 5569 SalI G'TCGA_C Cuts at: 1936 2911 1936 Size: 975 4594 SbfI CC_TGCA'GG Cuts at: 2921 2921 Size: 5569 ScaI AGT'ACT Cuts at: 3817 5529 3817 Size: 1712 3857 SfcI C'TryA_G Cuts at: 826 1045 2081 2917 4052 4733 4924 826 Size: 219 1036 836 1135 681 191 1471 Fragments arranged by size: 1471 1135 1036 836 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5569 SmaI CCC'GGG Cuts at: 1872 2896 1872 Size: 1024 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2060 3673 4574 4836 820 Size: 197 926 117 1613 901 262 1553 Fragments arranged by size: 1613 1553 926 901 262 197 117 SnaBI TAC'GTA Cuts at: 493 3360 493 Size: 2867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5569 SphI G_CATG'C Cuts at: 1132 2927 1132 Size: 1795 3774 SspI AAT'ATT Cuts at: 5 52 3493 5 Size: 47 3441 2081 Fragments arranged by size: 3441 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5569 StyI C'CwwG_G Cuts at: 513 1065 2349 2361 2827 3008 513 Size: 552 1284 12 466 181 3074 Fragments arranged by size: 3074 1284 552 466 181 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2186 2606 3596 3613 3766 3951 4396 Size: 1255 606 420 990 17 153 185 445 Cuts at: 4396 325 Size: 1498 Fragments arranged by size: 1498 1255 990 606 445 420 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3815 5527 96 Size: 274 80 33 51 1443 1838 1712 138 Fragments arranged by size: 1838 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2413 2445 2491 2523 5529 5561 2413 Size: 32 46 32 3006 32 2421 Fragments arranged by size: 3006 2421 46 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2123 1316 Size: 807 4762 XbaI T'CTAG_A Cuts at: 2905 2905 Size: 5569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2380 2149 Size: 231 5338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5569 XmaI C'CCGG_G Cuts at: 1870 2894 1870 Size: 1024 4545 XmnI GAAnn'nnTTC Cuts at: 3698 3698 Size: 5569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2758 3377 276 Size: 53 83 186 936 1224 619 2468 Fragments arranged by size: 2468 1224 936 619 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI