Restriction map for
pFN21AA1417
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII AccII AccIII AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaHI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KspI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 3739 GGTACC G^GTACC
AccI 3392 GTAGAC GT^[AC][GT]AC
AclI 2963 AACGTT AA^CGTT
AflIII 1877 ACATGT A^C[GA][CT]GT
AhdI 244 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2236 ATTAAT AT^TAAT
AsnI 2236 ATTAAT AT^TAAT
Asp718I 3739 GGTACC G^GTACC
AspEI 244 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 1312 GTCTTC GAAGAC(2/6)
BclI 2553 TGATCA T^GATCA
BpuAI 1312 GTCTTC GAAGAC(2/6)
BsaAI 2957 CACGTA [CT]AC^GT[GA]
BsaBI 528 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2780 ACCGGA [AT]^CCGG[AT]
BspHI 1689 TCATGA T^CATGA
BspLU11I 1877 ACATGT A^CATGT
BstAPI 540 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3047 CCAATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 562 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 674 GACTGAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 244 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2869 GATATC GAT^ATC
EcoT22I 3569 ATGCAT ATGCA^T
FbaI 2553 TGATCA T^GATCA
KpnI 3739 GGTACC GGTAC^C
MamI 528 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1162 CATATG CA^TATG
NsiI 3569 ATGCAT ATGCA^T
PflMI 3794 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2236 ATTAAT AT^TAAT
Psp1406I 2963 AACGTT AA^CGTT
RcaI 1689 TCATGA T^CATGA
SapI 2094 GAAGAGC GCTCTTC(1/4)
SmlI 1507 CTTGAG C^T[CT][GA]AG
StuI 101 AGGCCT AGG^CCT
Van91I 3794 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3783 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 796 CCGC CCGC(-3/-1)
4015 CCGC
AlwNI 3083 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
3815 CAGTGACTG
BcnI 2570 CCCGG CC^[GC]GG
3612 CCGGG
BseRI 983 CTCCTC GAGGAG(10/8)
3934 GAGGAG
EcoRI 1499 GAATTC G^AATTC
1992 GAATTC
HaeIII 102 GGCC GG^CC
1296 GGCC
HincII 1267 GTCAAC GT[CT]^[GA]AC
3075 GTTGAC
HindII 1267 GTCAAC GT[CT]^[GA]AC
3075 GTTGAC
MspA1I 704 CAGCTG C[AC]G^C[GT]G
1616 CAGCTG
NciI 2570 CCCGG CC^[GC]GG
3612 CCGGG
PstI 1579 CTGCAG CTGCA^G
1835 CTGCAG
PvuII 704 CAGCTG CAG^CTG
1616 CAGCTG
SphI 48 GCATGC GCATG^C
3939 GCATGC
SspI 2128 AATATT AAT^ATT
2375 AATATT
XcmI 2312 CCATGAAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3562 CCAGTGAATGCATGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
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AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BanI BbvI BfaI
BpmI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII EcoT14I
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI