(Circular) (Six-base) MAPSORT of: pFN21AA1417.seq Check: 4965 from: 1 to: 8764 pFN21AA1417 8764 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2536 2536 Size: 8764 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6574 278 Size: 53 83 186 936 5038 2468 Fragments arranged by size: 5038 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5398 6107 1937 Size: 3461 709 4594 Fragments arranged by size: 4594 3461 709 Acc65I G'GTAC_C Cuts at: 5744 6085 5744 Size: 341 8423 AclI AA'CG_TT Cuts at: 1563 4969 6891 7264 1563 Size: 3406 1922 373 3063 Fragments arranged by size: 3406 3063 1922 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2760 3305 3786 4174 4295 4643 5115 Size: 437 691 545 481 388 121 348 472 Cuts at: 5115 5802 6012 6827 8211 8479 1632 Size: 687 210 815 1384 268 1917 Fragments arranged by size: 1917 1384 815 691 687 545 481 472 437 388 348 268 210 121 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8567 AflIII A'CryG_T Cuts at: 1203 3882 7663 8640 1203 Size: 2679 3781 977 1327 Fragments arranged by size: 3781 2679 1327 977 AgeI A'CCGG_T Cuts at: 7595 7595 Size: 8764 AhdI GACnn_n'nnGTC Cuts at: 2254 7493 2254 Size: 5239 3525 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8764 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4188 1184 Size: 3004 5760 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4910 4942 4910 Size: 32 8732 AlwNI CAG_nnn'CTG Cuts at: 5093 5825 6507 8079 5093 Size: 732 682 1572 5778 Fragments arranged by size: 5778 1572 732 682 ApaLI G'TGCA_C Cuts at: 6821 7977 6821 Size: 1156 7608 ApoI r'AATT_y Cuts at: 1493 2458 2561 3219 3351 3504 3615 3806 3997 Size: 965 103 658 132 153 111 191 191 Cuts at: 3997 4018 4181 4340 4646 5704 6073 6299 6451 Size: 21 163 159 306 1058 369 226 152 Cuts at: 6451 8560 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1058 965 658 369 306 226 191 191 163 159 153 152 132 111 103 21 AseI AT'TA_AT Cuts at: 160 1032 4242 7318 160 Size: 872 3210 3076 1606 Fragments arranged by size: 3210 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8764 AvaI C'yCGr_G Cuts at: 1870 1943 6089 1870 Size: 73 4146 4545 Fragments arranged by size: 4545 4146 73 BamHI G'GATC_C Cuts at: 6094 6461 6094 Size: 367 8397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3846 5713 5744 6085 7540 Size: 325 217 627 2059 1867 31 341 1455 Cuts at: 7540 618 Size: 1842 Fragments arranged by size: 2059 1867 1842 1455 627 341 325 217 31 BanII G_rGCy'C Cuts at: 1391 6083 1391 Size: 4692 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3310 928 Size: 971 1411 6382 Fragments arranged by size: 6382 1411 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6955 6989 1903 Size: 34 5018 34 3678 Fragments arranged by size: 5018 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6656 7866 6656 Size: 1210 7554 BclI T'GATC_A Cuts at: 1548 4558 1548 Size: 3010 5754 BfrBI ATG'CAT Cuts at: 5576 5576 Size: 8764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7375 136 Size: 107 122 71 631 605 5703 1525 Fragments arranged by size: 5703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8759 8759 Size: 8764 BlpI GC'TnA_GC Cuts at: 6181 6181 Size: 8764 Bme1580I G_kGCm'C Cuts at: 1790 6825 7981 1790 Size: 5035 1156 2573 Fragments arranged by size: 5035 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3311 4312 1318 Size: 1993 1001 5770 Fragments arranged by size: 5770 1993 1001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2954 5283 5840 Size: 416 53 83 399 24 1537 2329 557 Cuts at: 5840 6495 7453 442 Size: 655 958 1753 Fragments arranged by size: 2329 1753 1537 958 655 557 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 2506 2640 3283 5004 7424 1239 Size: 862 405 134 643 1721 2420 2579 Fragments arranged by size: 2579 2420 1721 862 643 405 134 Bpu10I CC'TnA_GC Cuts at: 2715 2715 Size: 8764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3533 6889 7754 8052 3533 Size: 3356 865 298 4245 Fragments arranged by size: 4245 3356 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2319 3004 4740 7427 882 Size: 617 820 685 1736 2687 2219 Fragments arranged by size: 2687 2219 1736 820 685 617 BsaAI yAC'GTr Cuts at: 493 1204 4964 6555 493 Size: 711 3760 1591 2702 Fragments arranged by size: 3760 2702 1591 711 BsaBI GATnn'nnATC Cuts at: 2537 8758 2537 Size: 6221 2543 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6571 6953 7588 275 Size: 53 83 186 936 5038 382 635 1451 Fragments arranged by size: 5038 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4785 7197 7595 7869 8016 8427 4785 Size: 2412 398 274 147 411 5122 Fragments arranged by size: 5122 2412 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3045 3075 1178 Size: 30 165 30 1642 30 6867 Fragments arranged by size: 6867 1642 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2979 5954 1186 Size: 1793 2975 3996 Fragments arranged by size: 3996 2975 1793 BseYI C'CCAG_C Cuts at: 7967 8463 7967 Size: 496 8268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2011 3717 4449 1163 Size: 573 275 1706 732 5478 Fragments arranged by size: 5478 1706 732 573 275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6214 6975 8003 1540 Size: 275 186 4213 761 1028 2301 Fragments arranged by size: 4213 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6825 6910 7981 6825 Size: 85 1071 7608 Fragments arranged by size: 7608 1071 85 BsmI GAATG_Cn' Cuts at: 2170 3225 3690 3757 4633 5578 5593 6275 6368 Size: 1055 465 67 876 945 15 682 93 Cuts at: 6368 2170 Size: 4566 Fragments arranged by size: 4566 1055 945 876 682 465 93 67 15 Bsp1286I G_dGCh'C Cuts at: 1391 1790 6083 6825 6910 7981 1391 Size: 399 4293 742 85 1071 2174 Fragments arranged by size: 4293 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 8427 8427 Size: 8764 BspHI T'CATG_A Cuts at: 3694 6653 8383 3694 Size: 2959 1730 4075 Fragments arranged by size: 4075 2959 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2536 2584 2871 6119 844 Size: 1692 48 287 3248 3489 Fragments arranged by size: 3489 3248 1692 287 48 BsrBI CCG'CTC Cuts at: 1394 1823 6651 1394 Size: 429 4828 3507 Fragments arranged by size: 4828 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2270 3021 3240 4662 4809 7259 7433 Size: 1183 1022 751 219 1422 147 2450 174 Cuts at: 7433 65 Size: 1396 Fragments arranged by size: 2450 1422 1396 1183 1022 751 219 174 147 BsrFI r'CCGG_y Cuts at: 1700 1810 7408 7595 1700 Size: 110 5598 187 2869 Fragments arranged by size: 5598 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8764 BssSI C'ACGA_G Cuts at: 6824 7836 6824 Size: 1012 7752 BstAPI GCAn_nnn'nTGC Cuts at: 794 2551 794 Size: 1757 7007 BstBI TT'CG_AA Cuts at: 7568 7568 Size: 8764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8764 BstXI CCAn_nnnn'nTGG Cuts at: 5059 5059 Size: 8764 BstYI r'GATC_y Cuts at: 1972 2233 2374 2512 2959 3556 3592 4768 5365 Size: 261 141 138 447 597 36 1176 597 Cuts at: 5365 6094 6461 6846 6863 8315 8401 8413 8759 Size: 729 367 385 17 1452 86 12 346 Cuts at: 8759 1972 Size: 1977 Fragments arranged by size: 1977 1452 1176 729 597 597 447 385 367 346 261 141 138 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 5240 5740 5835 513 Size: 552 4175 500 95 3442 Fragments arranged by size: 4175 3442 552 500 95 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4767 487 Size: 683 56 3541 4484 Fragments arranged by size: 4484 3541 683 56 BtsI GCAGTG_nn' Cuts at: 3296 3759 6280 7074 7094 3296 Size: 463 2521 794 20 4966 Fragments arranged by size: 4966 2521 794 463 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8729 DraI TTT'AAA Cuts at: 3339 5235 5692 6068 3339 Size: 1896 457 376 6035 Fragments arranged by size: 6035 1896 457 376 DraIII CAC_nnn'GTG Cuts at: 2572 2572 Size: 8764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2685 7771 809 Size: 507 1369 5086 1802 Fragments arranged by size: 5086 1802 1369 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6211 7100 8729 Size: 54 1425 50 275 18 4381 889 1629 Cuts at: 8729 8 Size: 43 Fragments arranged by size: 4381 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6211 1537 Size: 275 4399 4090 Fragments arranged by size: 4399 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3396 3642 3731 4094 4807 5073 5988 6694 Size: 2043 246 89 363 713 266 915 706 Cuts at: 6694 1353 Size: 3423 Fragments arranged by size: 3423 2043 915 713 706 363 266 246 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7351 7725 7871 387 Size: 1064 138 5762 374 146 1280 Fragments arranged by size: 5762 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2101 2506 2640 2760 3283 3305 Size: 393 437 32 405 134 120 523 22 Cuts at: 3305 3786 4174 4295 4643 5004 5115 5802 6012 Size: 481 388 121 348 361 111 687 210 Cuts at: 6012 6827 7424 8211 8479 1239 Size: 815 597 787 268 1524 Fragments arranged by size: 1524 815 787 687 597 523 481 437 405 393 388 361 348 268 210 134 121 120 111 32 22 EcoRI G'AATT_C Cuts at: 3504 3997 6073 6451 3504 Size: 493 2076 378 5817 Fragments arranged by size: 5817 2076 493 378 EcoRV GAT'ATC Cuts at: 4876 4876 Size: 8764 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5912 5912 Size: 8764 FspI TGC'GCA Cuts at: 7270 7270 Size: 8764 HaeII r_GCGC'y Cuts at: 1678 7911 1678 Size: 6233 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2240 2272 2504 Size: 32 135 32 443 32 193 32 232 Cuts at: 2504 2536 3045 3077 3422 3454 5120 5152 7405 Size: 32 509 32 345 32 1666 32 2253 Cuts at: 7405 7437 7479 7511 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2253 1666 509 443 345 232 193 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3274 5082 6108 6354 669 Size: 988 281 1336 1808 1026 246 3079 Fragments arranged by size: 3079 1808 1336 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8764 HpaI GTT'AAC Cuts at: 6354 6354 Size: 8764 KpnI G_GTAC'C Cuts at: 5748 6089 5748 Size: 341 8423 MfeI C'AATT_G Cuts at: 6363 6363 Size: 8764 MluI A'CGCG_T Cuts at: 8640 8640 Size: 8764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7878 8062 1305 Size: 6573 184 2007 Fragments arranged by size: 6573 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6942 Fragments arranged by size: 6942 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2035 2640 4029 4303 4356 6722 7081 Size: 648 869 605 1389 274 53 2366 359 Cuts at: 7081 7240 518 Size: 159 2042 Fragments arranged by size: 2366 2042 1389 869 648 605 359 274 159 53 MspA1I CmG'CkG Cuts at: 1606 2711 3623 6180 6857 8005 8250 1606 Size: 1105 912 2557 677 1148 245 2120 Fragments arranged by size: 2557 2120 1148 1105 912 677 245 NcoI C'CATG_G Cuts at: 513 1065 5240 5740 5835 513 Size: 552 4175 500 95 3442 Fragments arranged by size: 4175 3442 552 500 95 NdeI CA'TA_TG Cuts at: 387 3168 387 Size: 2781 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8764 NotI GC'GGCC_GC Cuts at: 6211 6211 Size: 8764 NsiI A_TGCA'T Cuts at: 5578 5578 Size: 8764 NspI r_CATG'y Cuts at: 1132 1229 2057 2655 3886 5948 6122 7667 1132 Size: 97 828 598 1231 2062 174 1545 2229 Fragments arranged by size: 2229 2062 1545 1231 828 598 174 97 PasI CC'CwG_GG Cuts at: 1348 2467 1348 Size: 1119 7645 PciI A'CATG_T Cuts at: 3882 7663 3882 Size: 3781 4983 PflMI CCAn_nnn'nTGG Cuts at: 1108 5805 1108 Size: 4697 4067 PfoI T'CCnGG_A Cuts at: 2522 2617 5616 2522 Size: 95 2999 5670 Fragments arranged by size: 5670 2999 95 PmeI GTTT'AAAC Cuts at: 6068 6068 Size: 8764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6833 6865 8346 8378 6833 Size: 32 1481 32 7219 Fragments arranged by size: 7219 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8764 PsiI TTA'TAA Cuts at: 4992 6334 4992 Size: 1342 7422 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3855 3887 3855 Size: 32 8732 PstI C_TGCA'G Cuts at: 830 3588 3844 6116 830 Size: 2758 256 2272 3478 Fragments arranged by size: 3478 2758 2272 256 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8764 PvuII CAG'CTG Cuts at: 2711 3623 2711 Size: 912 7852 SalI G'TCGA_C Cuts at: 1936 6106 1936 Size: 4170 4594 SapI GCTCTTCn'nnn_ Cuts at: 4094 4094 Size: 8764 SbfI CC_TGCA'GG Cuts at: 6116 6116 Size: 8764 ScaI AGT'ACT Cuts at: 7012 8724 7012 Size: 1712 7052 SfcI C'TryA_G Cuts at: 826 1045 2427 3057 3584 3840 5025 6112 7247 Size: 219 1382 630 527 256 1185 1087 1135 Cuts at: 7247 7928 8119 826 Size: 681 191 1471 Fragments arranged by size: 1471 1382 1185 1135 1087 681 630 527 256 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8764 SmaI CCC'GGG Cuts at: 1872 6091 1872 Size: 4219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3512 6868 7769 8031 820 Size: 197 926 1569 3356 901 262 1553 Fragments arranged by size: 3356 1569 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6555 493 Size: 6062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8764 SphI G_CATG'C Cuts at: 1132 2057 5948 6122 1132 Size: 925 3891 174 3774 Fragments arranged by size: 3891 3774 925 174 SspI AAT'ATT Cuts at: 5 52 4135 4382 6688 5 Size: 47 4083 247 2306 2081 Fragments arranged by size: 4083 2306 2081 247 47 StuI AGG'CCT Cuts at: 1517 2108 1517 Size: 591 8173 StyI C'CwwG_G Cuts at: 513 1065 5240 5499 5740 5835 6203 513 Size: 552 4175 259 241 95 368 3074 Fragments arranged by size: 4175 3074 552 368 259 241 95 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2029 6791 6808 6961 7146 7591 325 Size: 1255 449 4762 17 153 185 445 1498 Fragments arranged by size: 4762 1498 1255 449 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7010 8722 96 Size: 274 80 33 51 1443 5033 1712 138 Fragments arranged by size: 5033 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2165 2197 8724 8756 2165 Size: 32 6527 32 2173 Fragments arranged by size: 6527 2173 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8764 XbaI T'CTAG_A Cuts at: 5788 6100 5788 Size: 312 8452 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4324 5574 4324 Size: 1250 7514 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8764 XmaI C'CCGG_G Cuts at: 1870 6089 1870 Size: 4219 4545 XmnI GAAnn'nnTTC Cuts at: 3415 3504 4383 5067 6893 3415 Size: 89 879 684 1826 5286 Fragments arranged by size: 5286 1826 879 684 89 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6572 276 Size: 53 83 186 936 5038 2468 Fragments arranged by size: 5038 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI