Restriction map for
pFN21AE0224
(Circular) (Six-base) MAPSORT of: pFN21AE0224.seq Check: 8468 from: 1 to: 4972
pFN21AE0224 4972 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2782 278
Size: 53 83 186 936 1246 2468
Fragments arranged by size:
2468 1246 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2315 1937
Size: 378 4594
Acc65I G'GTAC_C
Cuts at: 2293 2293
Size: 4972
AclI AA'CG_TT
Cuts at: 1563 3099 3472 1563
Size: 1536 373 3063
Fragments arranged by size:
3063 1536 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 3035 4419 4687 1632
Size: 404 999 1384 268 1917
Fragments arranged by size:
1917 1384 999 404 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4775
AflIII A'CryG_T
Cuts at: 1203 2120 3871 4848 1203
Size: 917 1751 977 1327
Fragments arranged by size:
1751 1327 977 917
AgeI A'CCGG_T
Cuts at: 3803 3803
Size: 4972
AhdI GACnn_n'nnGTC
Cuts at: 3701 3701
Size: 4972
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4972
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4972
AlwNI CAG_nnn'CTG
Cuts at: 2137 2715 4287 2137
Size: 578 1572 2822
Fragments arranged by size:
2822 1572 578
ApaLI G'TGCA_C
Cuts at: 3029 4185 3029
Size: 1156 3816
ApoI r'AATT_y
Cuts at: 1493 2143 2281 2507 2659 4768 1493
Size: 650 138 226 152 2109 1697
Fragments arranged by size:
2109 1697 650 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 3526 160
Size: 872 2494 1606
Fragments arranged by size:
2494 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4972
AvaI C'yCGr_G
Cuts at: 1870 1943 2297 1870
Size: 73 354 4545
Fragments arranged by size:
4545 354 73
BamHI G'GATC_C
Cuts at: 2201 2302 2669 2201
Size: 101 367 4504
Fragments arranged by size:
4504 367 101
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2293 3748 618
Size: 325 217 627 387 119 1455 1842
Fragments arranged by size:
1842 1455 627 387 325 217 119
BanII G_rGCy'C
Cuts at: 1391 2291 1391
Size: 900 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4001
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3163 3197 1903
Size: 34 1226 34 3678
Fragments arranged by size:
3678 1226 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2864 4074 2864
Size: 1210 3762
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4972
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3583 136
Size: 107 122 71 631 605 1911 1525
Fragments arranged by size:
1911 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4967 4967
Size: 4972
BlpI GC'TnA_GC
Cuts at: 2389 2389
Size: 4972
Bme1580I G_kGCm'C
Cuts at: 1790 3033 4189 1790
Size: 1243 1156 2573
Fragments arranged by size:
2573 1243 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4972
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2703 3661 442
Size: 416 53 83 399 24 1286 958 1753
Fragments arranged by size:
1753 1286 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3632 1239
Size: 2393 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3097 3962 4260 3097
Size: 865 298 3809
Fragments arranged by size:
3809 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3635 882
Size: 617 2136 2219
Fragments arranged by size:
2219 2136 617
BsaAI yAC'GTr
Cuts at: 493 1204 2763 493
Size: 711 1559 2702
Fragments arranged by size:
2702 1559 711
BsaBI GATnn'nnATC
Cuts at: 4966 4966
Size: 4972
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2779 3161 3796 275
Size: 53 83 186 936 1246 382 635 1451
Fragments arranged by size:
1451 1246 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3405 3803 4077 4224 4635 3405
Size: 398 274 147 411 3742
Fragments arranged by size:
3742 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4747
Fragments arranged by size:
4747 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4972
BseYI C'CCAG_C
Cuts at: 4175 4671 4175
Size: 496 4476
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2422 3183 4211 1540
Size: 275 186 421 761 1028 2301
Fragments arranged by size:
2301 1028 761 421 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 3033 3118 4189 2043
Size: 990 85 1071 2826
Fragments arranged by size:
2826 1071 990 85
BsmI GAATG_Cn'
Cuts at: 2230 2483 2576 2230
Size: 253 93 4626
Fragments arranged by size:
4626 253 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2291 3033 3118 4189 1391
Size: 399 253 248 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 253 248 85
BspEI T'CCGG_A
Cuts at: 4635 4635
Size: 4972
BspHI T'CATG_A
Cuts at: 2861 4591 2861
Size: 1730 3242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2327 844
Size: 1483 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2859 1394
Size: 429 1036 3507
Fragments arranged by size:
3507 1036 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3467 3641 65
Size: 1183 2219 174 1396
Fragments arranged by size:
2219 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3616 3803 1700
Size: 110 1806 187 2869
Fragments arranged by size:
2869 1806 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4972
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4972
BssSI C'ACGA_G
Cuts at: 3032 4044 3032
Size: 1012 3960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4972
BstBI TT'CG_AA
Cuts at: 3776 3776
Size: 4972
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4972
BstYI r'GATC_y
Cuts at: 1972 2201 2302 2669 3054 3071 4523 4609 4621
Size: 229 101 367 385 17 1452 86 12
Cuts at: 4621 4967 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 229 101 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3481
Fragments arranged by size:
3481 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4233
Fragments arranged by size:
4233 683 56
BtsI GCAGTG_nn'
Cuts at: 2112 2488 3282 3302 2112
Size: 376 794 20 3782
Fragments arranged by size:
3782 794 376 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4972
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4937
DraI TTT'AAA
Cuts at: 2241 2276 2241
Size: 35 4937
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3979 809
Size: 507 2663 1802
Fragments arranged by size:
2663 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2419 3308 4937
Size: 54 1425 50 275 18 589 889 1629
Cuts at: 4937 8
Size: 43
Fragments arranged by size:
1629 1425 889 589 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2419 1537
Size: 275 607 4090
Fragments arranged by size:
4090 607 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2902 1353
Size: 1549 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3559 3933 4079 387
Size: 1064 138 1970 374 146 1280
Fragments arranged by size:
1970 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 3035 3632 4419 4687 1239
Size: 393 404 999 597 787 268 1524
Fragments arranged by size:
1524 999 787 597 404 393 268
EcoRI G'AATT_C
Cuts at: 2281 2659 2281
Size: 378 4594
FspI TGC'GCA
Cuts at: 3478 3478
Size: 4972
HaeII r_GCGC'y
Cuts at: 1678 2137 4119 1678
Size: 459 1982 2531
Fragments arranged by size:
2531 1982 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3613 3645 3687 3719 1373
Size: 32 135 32 2041 32 42 32 2626
Fragments arranged by size:
2626 2041 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2316 2562 669
Size: 988 281 378 246 3079
Fragments arranged by size:
3079 988 378 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4972
HpaI GTT'AAC
Cuts at: 2562 2562
Size: 4972
KpnI G_GTAC'C
Cuts at: 2297 2297
Size: 4972
MfeI C'AATT_G
Cuts at: 2571 2571
Size: 4972
MluI A'CGCG_T
Cuts at: 4848 4848
Size: 4972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4086 4270 1305
Size: 2781 184 2007
Fragments arranged by size:
2781 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3150
Fragments arranged by size:
3150 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2930 3289 3448 518
Size: 648 1764 359 159 2042
Fragments arranged by size:
2042 1764 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2388 3065 4213 4458 1606
Size: 782 677 1148 245 2120
Fragments arranged by size:
2120 1148 782 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3481
Fragments arranged by size:
3481 939 552
NdeI CA'TA_TG
Cuts at: 387 2212 387
Size: 1825 3147
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4972
NotI GC'GGCC_GC
Cuts at: 2419 2419
Size: 4972
NspI r_CATG'y
Cuts at: 1132 1229 2124 2330 3875 1132
Size: 97 895 206 1545 2229
Fragments arranged by size:
2229 1545 895 206 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4972
PciI A'CATG_T
Cuts at: 2120 3871 2120
Size: 1751 3221
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4972
PmeI GTTT'AAAC
Cuts at: 2276 2276
Size: 4972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3041 3073 4554 4586 3041
Size: 32 1481 32 3427
Fragments arranged by size:
3427 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4972
PsiI TTA'TAA
Cuts at: 2542 2542
Size: 4972
PstI C_TGCA'G
Cuts at: 830 2324 830
Size: 1494 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4972
SalI G'TCGA_C
Cuts at: 1936 2314 1936
Size: 378 4594
SbfI CC_TGCA'GG
Cuts at: 2324 2324
Size: 4972
ScaI AGT'ACT
Cuts at: 3220 4932 3220
Size: 1712 3260
SfcI C'TryA_G
Cuts at: 826 1045 2320 3455 4136 4327 826
Size: 219 1275 1135 681 191 1471
Fragments arranged by size:
1471 1275 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4972
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4972
SmaI CCC'GGG
Cuts at: 1872 2299 1872
Size: 427 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3076 3977 4239 820
Size: 197 926 1133 901 262 1553
Fragments arranged by size:
1553 1133 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2763 493
Size: 2270 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4972
SphI G_CATG'C
Cuts at: 1132 2330 1132
Size: 1198 3774
SspI AAT'ATT
Cuts at: 5 52 2896 5
Size: 47 2844 2081
Fragments arranged by size:
2844 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4972
StyI C'CwwG_G
Cuts at: 513 1065 2004 2411 513
Size: 552 939 407 3074
Fragments arranged by size:
3074 939 552 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2089 2999 3016 3169 3354 3799 325
Size: 1255 509 910 17 153 185 445 1498
Fragments arranged by size:
1498 1255 910 509 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3218 4930 96
Size: 274 80 33 51 1443 1241 1712 138
Fragments arranged by size:
1712 1443 1241 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4932 4964 4932
Size: 32 4940
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4972
XbaI T'CTAG_A
Cuts at: 2308 2308
Size: 4972
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4972
XmaI C'CCGG_G
Cuts at: 1870 2297 1870
Size: 427 4545
XmnI GAAnn'nnTTC
Cuts at: 3101 3101
Size: 4972
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2780 276
Size: 53 83 186 936 1246 2468
Fragments arranged by size:
2468 1246 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI