(Circular) (Six-base) MAPSORT of: pFN21AB6308.seq Check: 6315 from: 1 to: 5545 pFN21AB6308 5545 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 278 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2888 1937 Size: 951 4594 Acc65I G'GTAC_C Cuts at: 2866 2866 Size: 5545 AclI AA'CG_TT Cuts at: 1563 3672 4045 1563 Size: 2109 373 3063 Fragments arranged by size: 3063 2109 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2709 2804 3608 4992 5260 1632 Size: 1077 95 804 1384 268 1917 Fragments arranged by size: 1917 1384 1077 804 268 95 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5348 AflIII A'CryG_T Cuts at: 1203 2811 4444 5421 1203 Size: 1608 1633 977 1327 Fragments arranged by size: 1633 1608 1327 977 AgeI A'CCGG_T Cuts at: 2449 4376 2449 Size: 1927 3618 AhdI GACnn_n'nnGTC Cuts at: 4274 4274 Size: 5545 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5545 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2439 2471 2439 Size: 32 5513 AlwNI CAG_nnn'CTG Cuts at: 2465 3288 4860 2465 Size: 823 1572 3150 Fragments arranged by size: 3150 1572 823 ApaLI G'TGCA_C Cuts at: 2693 3602 4758 2693 Size: 909 1156 3480 Fragments arranged by size: 3480 1156 909 ApoI r'AATT_y Cuts at: 1493 2201 2575 2854 3080 3232 5341 1493 Size: 708 374 279 226 152 2109 1697 Fragments arranged by size: 2109 1697 708 374 279 226 152 AseI AT'TA_AT Cuts at: 160 1032 4099 160 Size: 872 3067 1606 Fragments arranged by size: 3067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 AvaI C'yCGr_G Cuts at: 1870 1943 2271 2870 1870 Size: 73 328 599 4545 Fragments arranged by size: 4545 599 328 73 AvrII C'CTAG_G Cuts at: 2420 2420 Size: 5545 BamHI G'GATC_C Cuts at: 2875 3242 2875 Size: 367 5178 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2866 4321 618 Size: 325 217 627 1079 1455 1842 Fragments arranged by size: 1842 1455 1079 627 325 217 BanII G_rGCy'C Cuts at: 1391 2864 1391 Size: 1473 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2288 2501 2814 928 Size: 971 389 213 313 3659 Fragments arranged by size: 3659 971 389 313 213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3736 3770 1903 Size: 34 1799 34 3678 Fragments arranged by size: 3678 1799 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4647 3437 Size: 1210 4335 BclI T'GATC_A Cuts at: 1548 1548 Size: 5545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4156 136 Size: 107 122 71 631 605 2484 1525 Fragments arranged by size: 2484 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2623 5540 2623 Size: 2917 2628 BlpI GC'TnA_GC Cuts at: 2962 2962 Size: 5545 Bme1580I G_kGCm'C Cuts at: 1790 2697 3606 4762 1790 Size: 907 909 1156 2573 Fragments arranged by size: 2573 1156 909 907 BmgBI CAC'GTC Cuts at: 1318 2812 1318 Size: 1494 4051 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3276 4234 442 Size: 416 53 83 399 24 1859 958 1753 Fragments arranged by size: 1859 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5545 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 5545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4205 1239 Size: 2966 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3670 4535 4833 3670 Size: 865 298 4382 Fragments arranged by size: 4382 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2353 4208 882 Size: 617 854 1855 2219 Fragments arranged by size: 2219 1855 854 617 BsaAI yAC'GTr Cuts at: 493 1204 3336 493 Size: 711 2132 2702 Fragments arranged by size: 2702 2132 711 BsaBI GATnn'nnATC Cuts at: 5539 5539 Size: 5545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3352 3734 4369 275 Size: 53 83 186 936 1819 382 635 1451 Fragments arranged by size: 1819 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2449 3978 4376 4650 4797 5208 2449 Size: 1529 398 274 147 411 2786 Fragments arranged by size: 2786 1529 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5320 Fragments arranged by size: 5320 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2169 1186 Size: 983 4562 BseYI C'CCAG_C Cuts at: 4748 5244 4748 Size: 496 5049 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2077 1163 Size: 573 341 4631 Fragments arranged by size: 4631 573 341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2995 3756 4784 1540 Size: 275 186 994 761 1028 2301 Fragments arranged by size: 2301 1028 994 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2697 3606 3691 4762 2697 Size: 909 85 1071 3480 Fragments arranged by size: 3480 1071 909 85 BsmI GAATG_Cn' Cuts at: 2299 3056 3149 2299 Size: 757 93 4695 Fragments arranged by size: 4695 757 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2697 2864 3606 3691 4762 1391 Size: 399 907 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 907 742 399 167 85 BspEI T'CCGG_A Cuts at: 5208 5208 Size: 5545 BspHI T'CATG_A Cuts at: 2803 3434 5164 2803 Size: 631 1730 3184 Fragments arranged by size: 3184 1730 631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 2900 844 Size: 1255 801 3489 Fragments arranged by size: 3489 1255 801 BsrBI CCG'CTC Cuts at: 1394 1823 3432 1394 Size: 429 1609 3507 Fragments arranged by size: 3507 1609 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4040 4214 65 Size: 1183 2792 174 1396 Fragments arranged by size: 2792 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2449 4189 4376 1700 Size: 110 639 1740 187 2869 Fragments arranged by size: 2869 1740 639 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5545 BssSI C'ACGA_G Cuts at: 3605 4617 3605 Size: 1012 4533 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5545 BstBI TT'CG_AA Cuts at: 4349 4349 Size: 5545 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5545 BstXI CCAn_nnnn'nTGG Cuts at: 2026 2026 Size: 5545 BstYI r'GATC_y Cuts at: 1972 2461 2623 2875 3242 3627 3644 5096 5182 Size: 489 162 252 367 385 17 1452 86 Cuts at: 5182 5194 5540 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 489 385 367 346 252 162 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2352 513 Size: 552 1287 3706 Fragments arranged by size: 3706 1287 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4806 Fragments arranged by size: 4806 683 56 BtsI GCAGTG_nn' Cuts at: 3061 3855 3875 3061 Size: 794 20 4731 Fragments arranged by size: 4731 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5510 DraI TTT'AAA Cuts at: 2620 2849 2620 Size: 229 5316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4552 809 Size: 507 3236 1802 Fragments arranged by size: 3236 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2992 3881 5510 Size: 54 1425 50 275 18 1162 889 1629 Cuts at: 5510 8 Size: 43 Fragments arranged by size: 1629 1425 1162 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2992 1537 Size: 275 1180 4090 Fragments arranged by size: 4090 1180 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2498 3475 1353 Size: 1145 977 3423 Fragments arranged by size: 3423 1145 977 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4132 4506 4652 387 Size: 1064 138 2543 374 146 1280 Fragments arranged by size: 2543 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2709 2804 3608 4205 4992 5260 1239 Size: 393 1077 95 804 597 787 268 1524 Fragments arranged by size: 1524 1077 804 787 597 393 268 95 EcoO109I rG'GnC_Cy Cuts at: 2034 2034 Size: 5545 EcoRI G'AATT_C Cuts at: 2854 3232 2854 Size: 378 5167 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2140 2140 Size: 5545 FspI TGC'GCA Cuts at: 4051 4051 Size: 5545 HaeII r_GCGC'y Cuts at: 1678 4692 1678 Size: 3014 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 2348 2380 4186 Size: 32 135 32 668 32 76 32 1806 Cuts at: 4186 4218 4260 4292 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1806 668 135 76 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2889 3135 669 Size: 988 281 951 246 3079 Fragments arranged by size: 3079 988 951 281 246 HindIII A'AGCT_T Cuts at: 748 2078 2117 748 Size: 1330 39 4176 Fragments arranged by size: 4176 1330 39 HpaI GTT'AAC Cuts at: 3135 3135 Size: 5545 KpnI G_GTAC'C Cuts at: 2870 2870 Size: 5545 MfeI C'AATT_G Cuts at: 3144 3144 Size: 5545 MluI A'CGCG_T Cuts at: 5421 5421 Size: 5545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4659 4843 1305 Size: 3354 184 2007 Fragments arranged by size: 3354 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3723 Fragments arranged by size: 3723 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2411 3503 3862 4021 518 Size: 648 1245 1092 359 159 2042 Fragments arranged by size: 2042 1245 1092 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2961 3638 4786 5031 1606 Size: 1355 677 1148 245 2120 Fragments arranged by size: 2120 1355 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4993 NdeI CA'TA_TG Cuts at: 387 387 Size: 5545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5545 NotI GC'GGCC_GC Cuts at: 2992 2992 Size: 5545 NspI r_CATG'y Cuts at: 1132 1229 2167 2903 4448 1132 Size: 97 938 736 1545 2229 Fragments arranged by size: 2229 1545 938 736 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5545 PciI A'CATG_T Cuts at: 4444 4444 Size: 5545 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5545 PmeI GTTT'AAAC Cuts at: 2849 2849 Size: 5545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 3646 5127 5159 3614 Size: 32 1481 32 4000 Fragments arranged by size: 4000 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5545 PsiI TTA'TAA Cuts at: 3115 3115 Size: 5545 PstI C_TGCA'G Cuts at: 830 2227 2897 830 Size: 1397 670 3478 Fragments arranged by size: 3478 1397 670 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5545 SalI G'TCGA_C Cuts at: 1936 2887 1936 Size: 951 4594 SbfI CC_TGCA'GG Cuts at: 2897 2897 Size: 5545 ScaI AGT'ACT Cuts at: 3793 5505 3793 Size: 1712 3833 SfcI C'TryA_G Cuts at: 826 1045 2223 2717 2893 4028 4709 4900 826 Size: 219 1178 494 176 1135 681 191 1471 Fragments arranged by size: 1471 1178 1135 681 494 219 191 176 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5545 SmaI CCC'GGG Cuts at: 1872 2872 1872 Size: 1000 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2271 3649 4550 4812 820 Size: 197 926 328 1378 901 262 1553 Fragments arranged by size: 1553 1378 926 901 328 262 197 SnaBI TAC'GTA Cuts at: 493 3336 493 Size: 2843 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5545 SphI G_CATG'C Cuts at: 1132 2903 1132 Size: 1771 3774 SspI AAT'ATT Cuts at: 5 52 2767 3469 5 Size: 47 2715 702 2081 Fragments arranged by size: 2715 2081 702 47 StuI AGG'CCT Cuts at: 1517 2756 1517 Size: 1239 4306 StyI C'CwwG_G Cuts at: 513 1065 2420 2984 513 Size: 552 1355 564 3074 Fragments arranged by size: 3074 1355 564 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2672 3572 3589 3742 3927 4372 325 Size: 1255 1092 900 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1092 900 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3791 5503 96 Size: 274 80 33 51 1443 1814 1712 138 Fragments arranged by size: 1814 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5505 5537 5505 Size: 32 5513 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5545 XbaI T'CTAG_A Cuts at: 2881 2881 Size: 5545 XhoI C'TCGA_G Cuts at: 1943 2271 1943 Size: 328 5217 XmaI C'CCGG_G Cuts at: 1870 2870 1870 Size: 1000 4545 XmnI GAAnn'nnTTC Cuts at: 2048 2192 3674 2048 Size: 144 1482 3919 Fragments arranged by size: 3919 1482 144 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 276 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI