Restriction map for
pFN21AB8708
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
ApaI ApaLI AscI Asp700I AspEI
AvrII BalI BamHI BbeI BbrPI
BbsI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
HgaI Hin1I HincII HindII HpaI
KasI KspI MflI MluI MluNI
MroI MscI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 940 GGTACC G^GTACC
AcsI 235 AAATTT [GA]^AATT[CT]
AflIII 585 ACATGT A^C[GA][CT]GT
AocI 352 CCTGAGG CC^T[ACGT]AGG
Aor51HI 963 AGCGCT AGC^GCT
ApoI 235 AAATTT [GA]^AATT[CT]
AseI 289 ATTAAT AT^TAAT
AsnI 289 ATTAAT AT^TAAT
Asp718I 940 GGTACC G^GTACC
AspI 822 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1312 CCCGGG C^[CT]CG[GA]G
AviII 794 TGCGCA TGC^GCA
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BclI 1782 TGATCA T^GATCA
BfaI 850 CTAG C^TAG
BglI 1881 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 127 TACGTG [CT]AC^GT[GA]
BsaBI 1506 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1216 GAGACG CGTCTC(1/5)
BsmI 1623 GAATGC GAATGC(1/-1)
BsoBI 1312 CCCGGG C^[CT]CG[GA]G
Bsp1407I 252 TGTACA T^GTACA
BspLU11I 585 ACATGT A^CATGT
BsrBI 148 GAGCGG CCGCTC(-3/-3)
BsrFI 1489 ACCGGC [GA]^CCGG[CT]
BsrGI 252 TGTACA T^GTACA
Bsu36I 352 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1489 ACCGGC [GA]^CCGG[CT]
DraIII 724 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 822 GACCCTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 167 CGGCCA [CT]^GGCC[GA]
Eco47III 963 AGCGCT AGC^GCT
Eco81I 352 CCTGAGG CC^T[ACGT]AGG
FauI 1028 CCCGC CCCGC(4/6)
FbaI 1782 TGATCA T^GATCA
FspI 794 TGCGCA TGC^GCA
HaeII 963 AGCGCT [GA]GCGC^[CT]
HindIII 341 AAGCTT A^AGCTT
KpnI 940 GGTACC GGTAC^C
MaeI 850 CTAG C^TAG
MamI 1506 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1907 CAATTG C^AATTG
MspA1I 1045 CAGCGG C[AC]G^C[GT]G
MunI 1907 CAATTG C^AATTG
NcoI 1475 CCATGG C^CATGG
NdeI 1115 CATATG CA^TATG
NspI 585 ACATGT [GA]CATG^[CT]
PflFI 822 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 723 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 289 ATTAAT AT^TAAT
SexAI 176 ACCAGGT A^CC[AT]GGT
SfcI 953 CTGTAG C^T[GA][CT]AG
SmaI 1312 CCCGGG CCC^GGG
SspBI 252 TGTACA T^GTACA
SspI 1713 AATATT AAT^ATT
Tth111I 822 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 723 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1312 CCCGGG C^CCGGG
XmaI 1312 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 244 GATCC GGATC(4/5)
1323 GATCC
AlwNI 1045 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1171 CAGGACCTG
BanI 40 GGCACC G^G[CT][GA]CC
940 GGTACC
BcnI 1312 CCCGG CC^[GC]GG
1313 CCGGG
BseRI 733 GAGGAG GAGGAG(10/8)
1543 GAGGAG
BsgI 923 GTGCAG GTGCAG(16/14)
1042 GTGCAG
BsmAI 391 GAGAC GTCTC(1/5)
1216 GAGAC
BsrDI 546 CATTGC GCAATG(2/0)
184 GCAATG
BstXI 122 CCAGCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1139 CCATCCTGATGG
DsaI 533 CCACGG C^C[GA][CT]GG
1475 CCATGG
HapII 1313 CCGG C^CGG
1490 CCGG
HpaII 1313 CCGG C^CGG
1490 CCGG
MspI 1313 CCGG C^CGG
1490 CCGG
NciI 1312 CCCGG CC^[GC]GG
1313 CCGGG
PleI 460 GACTC GAGTC(4/5)
1696 GAGTC
PpuMI 820 AGGACCC [GA]G^G[AT]CC[CT]
1172 AGGACCT
SacI 902 GAGCTC GAGCT^C
1732 GAGCTC
StuI 401 AGGCCT AGG^CCT
1888 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I