(Circular) (Six-base) MAPSORT of: pFN21AB8708.seq Check: 8312 from: 1 to: 6628 pFN21AB8708 6628 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2483 2483 Size: 6628 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4438 278 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3971 1937 Size: 2034 4594 Acc65I G'GTAC_C Cuts at: 2945 3949 2945 Size: 1004 5624 AclI AA'CG_TT Cuts at: 1563 4755 5128 1563 Size: 3192 373 3063 Fragments arranged by size: 3192 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3044 3612 4691 6075 6343 1632 Size: 1412 568 1079 1384 268 1917 Fragments arranged by size: 1917 1412 1384 1079 568 268 AfeI AGC'GCT Cuts at: 2970 2970 Size: 6628 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6431 AflIII A'CryG_T Cuts at: 1203 2590 5527 6504 1203 Size: 1387 2937 977 1327 Fragments arranged by size: 2937 1387 1327 977 AgeI A'CCGG_T Cuts at: 5459 5459 Size: 6628 AhdI GACnn_n'nnGTC Cuts at: 5357 5357 Size: 6628 AleI CACnn'nnGTG Cuts at: 1166 2694 1166 Size: 1528 5100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6628 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2577 2609 2577 Size: 32 6596 AlwNI CAG_nnn'CTG Cuts at: 3055 3181 4371 5943 3055 Size: 126 1190 1572 3740 Fragments arranged by size: 3740 1572 1190 126 ApaLI G'TGCA_C Cuts at: 4685 5841 4685 Size: 1156 5472 ApoI r'AATT_y Cuts at: 1493 2240 3937 4163 4315 6424 1493 Size: 747 1697 226 152 2109 1697 Fragments arranged by size: 2109 1697 1697 747 226 152 AseI AT'TA_AT Cuts at: 160 1032 2295 5182 160 Size: 872 1263 2887 1606 Fragments arranged by size: 2887 1606 1263 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 AvaI C'yCGr_G Cuts at: 1870 1943 3317 3953 1870 Size: 73 1374 636 4545 Fragments arranged by size: 4545 1374 636 73 BamHI G'GATC_C Cuts at: 3958 4325 3958 Size: 367 6261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2945 3949 5404 618 Size: 325 217 627 258 900 1004 1455 1842 Fragments arranged by size: 1842 1455 1004 900 627 325 258 217 BanII G_rGCy'C Cuts at: 1391 2538 2911 3029 3741 3947 1391 Size: 1147 373 118 712 206 4072 Fragments arranged by size: 4072 1147 712 373 206 118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5657 BbvCI CC'TCA_GC Cuts at: 2403 2403 Size: 6628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4819 4853 1903 Size: 34 2882 34 3678 Fragments arranged by size: 3678 2882 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4520 5730 4520 Size: 1210 5418 BclI T'GATC_A Cuts at: 1548 3787 1548 Size: 2239 4389 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3892 5239 136 Size: 107 122 71 631 605 2220 1347 1525 Fragments arranged by size: 2220 1525 1347 631 605 122 107 71 BglII A'GATC_T Cuts at: 6623 6623 Size: 6628 BlpI GC'TnA_GC Cuts at: 4045 4045 Size: 6628 Bme1580I G_kGCm'C Cuts at: 1790 2048 4689 5845 1790 Size: 258 2641 1156 2573 Fragments arranged by size: 2641 2573 1156 258 BmgBI CAC'GTC Cuts at: 1318 3080 3196 1318 Size: 1762 116 4750 Fragments arranged by size: 4750 1762 116 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3867 4359 5317 Size: 416 53 83 399 24 2450 492 958 Cuts at: 5317 442 Size: 1753 Fragments arranged by size: 2450 1753 958 492 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6628 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 6628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2972 3239 3390 3773 5288 1239 Size: 1733 267 151 383 1515 2579 Fragments arranged by size: 2579 1733 1515 383 267 151 Bpu10I CC'TnA_GC Cuts at: 2403 3539 2403 Size: 1136 5492 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4753 5618 5916 4753 Size: 865 298 5465 Fragments arranged by size: 5465 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5291 882 Size: 617 3792 2219 Fragments arranged by size: 3792 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2134 4419 493 Size: 711 930 2285 2702 Fragments arranged by size: 2702 2285 930 711 BsaBI GATnn'nnATC Cuts at: 3515 6622 3515 Size: 3107 3521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4435 4817 5452 275 Size: 53 83 186 936 2902 382 635 1451 Fragments arranged by size: 2902 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5061 5459 5733 5880 6291 5061 Size: 398 274 147 411 5398 Fragments arranged by size: 5398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3800 3830 1178 Size: 30 165 30 2397 30 3976 Fragments arranged by size: 3976 2397 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2753 3563 1186 Size: 1567 810 4251 Fragments arranged by size: 4251 1567 810 BseYI C'CCAG_C Cuts at: 2126 2678 3227 3376 5831 6327 2126 Size: 552 549 149 2455 496 2427 Fragments arranged by size: 2455 2427 552 549 496 149 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2949 3068 1163 Size: 573 1213 119 4723 Fragments arranged by size: 4723 1213 573 119 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4078 4839 5867 1540 Size: 275 186 2077 761 1028 2301 Fragments arranged by size: 2301 2077 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2911 3494 3741 4689 4774 5845 2911 Size: 583 247 948 85 1071 3694 Fragments arranged by size: 3694 1071 948 583 247 85 BsmI GAATG_Cn' Cuts at: 3634 4139 4232 3634 Size: 505 93 6030 Fragments arranged by size: 6030 505 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3215 3215 Size: 6628 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2538 2911 3029 3494 3741 3947 Size: 399 258 490 373 118 465 247 206 Cuts at: 3947 4689 4774 5845 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 465 399 373 258 247 206 118 85 BspEI T'CCGG_A Cuts at: 6291 6291 Size: 6628 BspHI T'CATG_A Cuts at: 4517 6247 4517 Size: 1730 4898 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2483 3189 3381 3983 844 Size: 1639 706 192 602 3489 Fragments arranged by size: 3489 1639 706 602 192 BsrBI CCG'CTC Cuts at: 1394 1823 2155 4515 1394 Size: 429 332 2360 3507 Fragments arranged by size: 3507 2360 429 332 BsrDI GCAATG_nn' Cuts at: 65 1248 2196 2550 5123 5297 65 Size: 1183 948 354 2573 174 1396 Fragments arranged by size: 2573 1396 1183 948 354 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3494 5272 5459 1700 Size: 110 1684 1778 187 2869 Fragments arranged by size: 2869 1778 1684 187 110 BsrGI T'GTAC_A Cuts at: 96 2257 96 Size: 2161 4467 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6628 BssSI C'ACGA_G Cuts at: 4688 5700 4688 Size: 1012 5616 BstAPI GCAn_nnn'nTGC Cuts at: 794 2808 3055 3629 794 Size: 2014 247 574 3793 Fragments arranged by size: 3793 2014 574 247 BstBI TT'CG_AA Cuts at: 5432 5432 Size: 6628 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6628 BstXI CCAn_nnnn'nTGG Cuts at: 2134 3151 2134 Size: 1017 5611 BstYI r'GATC_y Cuts at: 1972 3958 4325 4710 4727 6179 6265 6277 6623 Size: 1986 367 385 17 1452 86 12 346 Cuts at: 6623 1972 Size: 1977 Fragments arranged by size: 1986 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2358 2358 Size: 6628 BtgI C'CryG_G Cuts at: 513 1065 2004 2538 3480 513 Size: 552 939 534 942 3661 Fragments arranged by size: 3661 942 939 552 534 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2080 3502 487 Size: 683 56 854 1422 3613 Fragments arranged by size: 3613 1422 854 683 56 BtsI GCAGTG_nn' Cuts at: 2442 2673 4144 4938 4958 2442 Size: 231 1471 794 20 4112 Fragments arranged by size: 4112 1471 794 231 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6628 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6593 DraI TTT'AAA Cuts at: 3932 3932 Size: 6628 DraIII CAC_nnn'GTG Cuts at: 2734 2734 Size: 6628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2833 5635 809 Size: 507 1517 2802 1802 Fragments arranged by size: 2802 1802 1517 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 4075 4964 Size: 54 1425 50 275 18 342 1903 889 Cuts at: 4964 6593 8 Size: 1629 43 Fragments arranged by size: 1903 1629 1425 889 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4075 1537 Size: 275 2263 4090 Fragments arranged by size: 4090 2263 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2374 2913 3653 4558 1353 Size: 1021 539 740 905 3423 Fragments arranged by size: 3423 1021 905 740 539 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5215 5589 5735 387 Size: 1064 138 3626 374 146 1280 Fragments arranged by size: 3626 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2909 3739 2909 Size: 830 5798 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2972 3044 3239 3390 3612 3773 4691 Size: 393 1340 72 195 151 222 161 918 Cuts at: 4691 5288 6075 6343 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1340 918 787 597 393 268 222 195 161 151 72 EcoNI CCTnn'n_nnAGG Cuts at: 2812 3542 3900 2812 Size: 730 358 5540 Fragments arranged by size: 5540 730 358 EcoO109I rG'GnC_Cy Cuts at: 2826 3178 3905 2826 Size: 352 727 5549 Fragments arranged by size: 5549 727 352 EcoRI G'AATT_C Cuts at: 3937 4315 3937 Size: 378 6250 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3649 3649 Size: 6628 FspI TGC'GCA Cuts at: 2801 5134 2801 Size: 2333 4295 HaeII r_GCGC'y Cuts at: 1678 2972 5775 1678 Size: 1294 2803 2531 Fragments arranged by size: 2803 2531 1294 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2590 2622 3069 3101 5269 Size: 32 135 32 1018 32 447 32 2168 Cuts at: 5269 5301 5343 5375 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2168 1018 447 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3972 4218 669 Size: 988 281 2034 246 3079 Fragments arranged by size: 3079 2034 988 281 246 HindIII A'AGCT_T Cuts at: 748 2346 748 Size: 1598 5030 HpaI GTT'AAC Cuts at: 4218 4218 Size: 6628 KpnI G_GTAC'C Cuts at: 2949 3953 2949 Size: 1004 5624 MfeI C'AATT_G Cuts at: 3912 4227 3912 Size: 315 6313 MluI A'CGCG_T Cuts at: 6504 6504 Size: 6628 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5742 5926 1305 Size: 4437 184 2007 Fragments arranged by size: 4437 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4806 Fragments arranged by size: 4806 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2694 3149 3479 4586 4945 5104 518 Size: 648 1528 455 330 1107 359 159 2042 Fragments arranged by size: 2042 1528 1107 648 455 359 330 159 MspA1I CmG'CkG Cuts at: 1606 3052 4044 4721 5869 6114 1606 Size: 1446 992 677 1148 245 2120 Fragments arranged by size: 2120 1446 1148 992 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3480 513 Size: 552 939 1476 3661 Fragments arranged by size: 3661 1476 939 552 NdeI CA'TA_TG Cuts at: 387 3121 387 Size: 2734 3894 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6628 NotI GC'GGCC_GC Cuts at: 4075 4075 Size: 6628 NspI r_CATG'y Cuts at: 1132 1229 2594 3986 5531 1132 Size: 97 1365 1392 1545 2229 Fragments arranged by size: 2229 1545 1392 1365 97 PasI CC'CwG_GG Cuts at: 1348 3211 3414 1348 Size: 1863 203 4562 Fragments arranged by size: 4562 1863 203 PciI A'CATG_T Cuts at: 2590 5527 2590 Size: 2937 3691 PflMI CCAn_nnn'nTGG Cuts at: 1108 2734 1108 Size: 1626 5002 PmeI GTTT'AAAC Cuts at: 3932 3932 Size: 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4697 4729 6210 6242 4697 Size: 32 1481 32 5083 Fragments arranged by size: 5083 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2826 3178 2826 Size: 352 6276 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6628 PsiI TTA'TAA Cuts at: 4198 4198 Size: 6628 PstI C_TGCA'G Cuts at: 830 3980 830 Size: 3150 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6628 SacI G_AGCT'C Cuts at: 2911 3741 2911 Size: 830 5798 SalI G'TCGA_C Cuts at: 1936 3970 1936 Size: 2034 4594 SbfI CC_TGCA'GG Cuts at: 3980 3980 Size: 6628 ScaI AGT'ACT Cuts at: 4876 6588 4876 Size: 1712 4916 SexAI A'CCwGG_T Cuts at: 2181 2181 Size: 6628 SfcI C'TryA_G Cuts at: 826 1045 2958 3976 5111 5792 5983 826 Size: 219 1913 1018 1135 681 191 1471 Fragments arranged by size: 1913 1471 1135 1018 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6628 SmaI CCC'GGG Cuts at: 1872 3319 3955 1872 Size: 1447 636 4545 Fragments arranged by size: 4545 1447 636 SmlI C'TyrA_G Cuts at: 820 1017 1943 4732 5633 5895 820 Size: 197 926 2789 901 262 1553 Fragments arranged by size: 2789 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4419 493 Size: 3926 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6628 SphI G_CATG'C Cuts at: 1132 3986 1132 Size: 2854 3774 SspI AAT'ATT Cuts at: 5 52 3720 4552 5 Size: 47 3668 832 2081 Fragments arranged by size: 3668 2081 832 47 StuI AGG'CCT Cuts at: 1517 2408 3895 1517 Size: 891 1487 4250 Fragments arranged by size: 4250 1487 891 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 3042 3480 3844 4067 513 Size: 552 939 12 1026 438 364 223 3074 Fragments arranged by size: 3074 1026 939 552 438 364 223 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2600 2684 2858 3427 4655 4672 4825 Size: 1255 1020 84 174 569 1228 17 153 Cuts at: 4825 5010 5455 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1228 1020 569 445 185 174 153 84 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2257 4874 6586 Size: 274 80 33 51 1443 280 2617 1712 Cuts at: 6586 96 Size: 138 Fragments arranged by size: 2617 1712 1443 280 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2499 2531 3800 3832 6588 6620 2499 Size: 32 1269 32 2756 32 2507 Fragments arranged by size: 2756 2507 1269 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2830 1316 Size: 1514 5114 XbaI T'CTAG_A Cuts at: 3964 3964 Size: 6628 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6628 XmaI C'CCGG_G Cuts at: 1870 3317 3953 1870 Size: 1447 636 4545 Fragments arranged by size: 4545 1447 636 XmnI GAAnn'nnTTC Cuts at: 4757 4757 Size: 6628 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4436 276 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI