Restriction map for
pFN21AB8708
(Linear) (Six-base) MAPSORT of: KIBB8708.seq Check: 8126 from: 1 to: 1923
KIBB8708.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 478 1923
Size: 478 1445
Acc65I G'GTAC_C
Cuts at: 0 940 1923
Size: 940 983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1607 1923
Size: 1039 568 316
Fragments arranged by size:
1039 568 316
AfeI AGC'GCT
Cuts at: 0 965 1923
Size: 965 958
AflIII A'CryG_T
Cuts at: 0 585 1923
Size: 585 1338
AleI CACnn'nnGTG
Cuts at: 0 689 1923
Size: 689 1234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 572 604 1923
Size: 572 32 1319
Fragments arranged by size:
1319 572 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1050 1176 1923
Size: 1050 126 747
Fragments arranged by size:
1050 747 126
ApoI r'AATT_y
Cuts at: 0 235 1923
Size: 235 1688
AseI AT'TA_AT
Cuts at: 0 290 1923
Size: 290 1633
AvaI C'yCGr_G
Cuts at: 0 1312 1923
Size: 1312 611
BanI G'GyrC_C
Cuts at: 0 40 940 1923
Size: 40 900 983
Fragments arranged by size:
983 900 40
BanII G_rGCy'C
Cuts at: 0 533 906 1024 1736 1923
Size: 533 373 118 712 187
Fragments arranged by size:
712 533 373 187 118
BbvCI CC'TCA_GC
Cuts at: 0 398 1923
Size: 398 1525
BclI T'GATC_A
Cuts at: 0 1782 1923
Size: 1782 141
BglI GCCn_nnn'nGGC
Cuts at: 0 1887 1923
Size: 1887 36
Bme1580I G_kGCm'C
Cuts at: 0 43 1923
Size: 43 1880
BmgBI CAC'GTC
Cuts at: 0 1075 1191 1923
Size: 1075 116 732
Fragments arranged by size:
1075 732 116
BmrI ACTGGGnnnn_n'
Cuts at: 0 1862 1923
Size: 1862 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 1923
Size: 378 1545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1234 1385 1768 1923
Size: 967 267 151 383 155
Fragments arranged by size:
967 383 267 155 151
Bpu10I CC'TnA_GC
Cuts at: 0 398 1534 1923
Size: 398 1136 389
Fragments arranged by size:
1136 398 389
BsaAI yAC'GTr
Cuts at: 0 129 1923
Size: 129 1794
BsaBI GATnn'nnATC
Cuts at: 0 1510 1923
Size: 1510 413
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1795 1825 1923
Size: 1795 30 98
Fragments arranged by size:
1795 98 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 748 1558 1923
Size: 748 810 365
Fragments arranged by size:
810 748 365
BseYI C'CCAG_C
Cuts at: 0 121 673 1222 1371 1923
Size: 121 552 549 149 552
Fragments arranged by size:
552 552 549 149 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1063 1923
Size: 944 119 860
Fragments arranged by size:
944 860 119
BsiHKAI G_wGCw'C
Cuts at: 0 906 1489 1736 1923
Size: 906 583 247 187
Fragments arranged by size:
906 583 247 187
BsmI GAATG_Cn'
Cuts at: 0 1629 1923
Size: 1629 294
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1210 1923
Size: 1210 713
Bsp1286I G_dGCh'C
Cuts at: 0 43 533 906 1024 1489 1736 1923
Size: 43 490 373 118 465 247 187
Fragments arranged by size:
490 465 373 247 187 118 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 478 1184 1376 1923
Size: 478 706 192 547
Fragments arranged by size:
706 547 478 192
BsrBI CCG'CTC
Cuts at: 0 150 1923
Size: 150 1773
BsrDI GCAATG_nn'
Cuts at: 0 191 545 1923
Size: 191 354 1378
Fragments arranged by size:
1378 354 191
BsrFI r'CCGG_y
Cuts at: 0 1489 1923
Size: 1489 434
BsrGI T'GTAC_A
Cuts at: 0 252 1923
Size: 252 1671
BstAPI GCAn_nnn'nTGC
Cuts at: 0 803 1050 1624 1923
Size: 803 247 574 299
Fragments arranged by size:
803 574 299 247
BstXI CCAn_nnnn'nTGG
Cuts at: 0 129 1146 1923
Size: 129 1017 777
Fragments arranged by size:
1017 777 129
Bsu36I CC'TnA_GG
Cuts at: 0 353 1923
Size: 353 1570
BtgI C'CryG_G
Cuts at: 0 533 1475 1923
Size: 533 942 448
Fragments arranged by size:
942 533 448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 75 1497 1923
Size: 75 1422 426
Fragments arranged by size:
1422 426 75
BtsI GCAGTG_nn'
Cuts at: 0 437 668 1923
Size: 437 231 1255
Fragments arranged by size:
1255 437 231
DraIII CAC_nnn'GTG
Cuts at: 0 729 1923
Size: 729 1194
DrdI GACnn_nn'nnGTC
Cuts at: 0 828 1923
Size: 828 1095
EaeI y'GGCC_r
Cuts at: 0 167 1923
Size: 167 1756
EarI CTCTTCn'nnn_
Cuts at: 0 369 908 1648 1923
Size: 369 539 740 275
Fragments arranged by size:
740 539 369 275
EcoICRI GAG'CTC
Cuts at: 0 904 1734 1923
Size: 904 830 189
Fragments arranged by size:
904 830 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1039 1234 1385 1607 1768 1923
Size: 967 72 195 151 222 161 155
Fragments arranged by size:
967 222 195 161 155 151 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 807 1537 1895 1923
Size: 807 730 358 28
Fragments arranged by size:
807 730 358 28
EcoO109I rG'GnC_Cy
Cuts at: 0 821 1173 1900 1923
Size: 821 352 727 23
Fragments arranged by size:
821 727 352 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1644 1923
Size: 1644 279
FspI TGC'GCA
Cuts at: 0 796 1923
Size: 796 1127
HaeII r_GCGC'y
Cuts at: 0 967 1923
Size: 967 956
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 585 617 1064 1096 1923
Size: 585 32 447 32 827
Fragments arranged by size:
827 585 447 32 32
HindIII A'AGCT_T
Cuts at: 0 341 1923
Size: 341 1582
KpnI G_GTAC'C
Cuts at: 0 944 1923
Size: 944 979
MfeI C'AATT_G
Cuts at: 0 1907 1923
Size: 1907 16
MslI CAynn'nnrTG
Cuts at: 0 689 1144 1474 1923
Size: 689 455 330 449
Fragments arranged by size:
689 455 449 330
MspA1I CmG'CkG
Cuts at: 0 1047 1923
Size: 1047 876
NcoI C'CATG_G
Cuts at: 0 1475 1923
Size: 1475 448
NdeI CA'TA_TG
Cuts at: 0 1116 1923
Size: 1116 807
NspI r_CATG'y
Cuts at: 0 589 1923
Size: 589 1334
PasI CC'CwG_GG
Cuts at: 0 1206 1409 1923
Size: 1206 203 514
Fragments arranged by size:
1206 514 203
PciI A'CATG_T
Cuts at: 0 585 1923
Size: 585 1338
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 1923
Size: 729 1194
PpuMI rG'GwC_Cy
Cuts at: 0 821 1173 1923
Size: 821 352 750
Fragments arranged by size:
821 750 352
SacI G_AGCT'C
Cuts at: 0 906 1736 1923
Size: 906 830 187
Fragments arranged by size:
906 830 187
SexAI A'CCwGG_T
Cuts at: 0 176 1923
Size: 176 1747
SfcI C'TryA_G
Cuts at: 0 953 1923
Size: 953 970
SmaI CCC'GGG
Cuts at: 0 1314 1923
Size: 1314 609
SspI AAT'ATT
Cuts at: 0 1715 1923
Size: 1715 208
StuI AGG'CCT
Cuts at: 0 403 1890 1923
Size: 403 1487 33
Fragments arranged by size:
1487 403 33
StyI C'CwwG_G
Cuts at: 0 11 1037 1475 1839 1923
Size: 11 1026 438 364 84
Fragments arranged by size:
1026 438 364 84 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 679 853 1422 1923
Size: 595 84 174 569 501
Fragments arranged by size:
595 569 501 174 84
TatI w'GTAC_w
Cuts at: 0 252 1923
Size: 252 1671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 494 526 1795 1827 1923
Size: 494 32 1269 32 96
Fragments arranged by size:
1269 494 96 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 825 1923
Size: 825 1098
XmaI C'CCGG_G
Cuts at: 0 1312 1923
Size: 1312 611
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AloI AlwNI
ApoI AseI AvaI BanI BanII BbvCI BclI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII
KpnI MfeI MslI MspA1I NcoI NdeI NspI PasI
PciI PflMI PpuMI SacI SexAI SfcI SmaI SspI
StuI StyI TaqII TatI TstI Tth111I XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AlfI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbsI
BcgI BciVI BfrBI BglII BlpI BmtI BpuEI BsaI
BsaHI BsaWI BsiEI BsiWI BspEI BspHI BssHII BssSI
BstBI BstEII BstYI BstZ17I ClaI CspCI DraI EagI
EciI EcoRI EcoRV FseI FspAI HincII HpaI KasI
MluI MmeI MscI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SwaI
XbaI XcmI XhoI XmnI ZraI