################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI Bsp1407I BspEI BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I FauI FspI HindIII HpaI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 720 GGTACC G^GTACC AccI 407 GTCTAC GT^[AC][GT]AC AcyI 144 GGCGCC G[GA]^CG[CT]C AocI 61 CCTGAGG CC^T[ACGT]AGG ApaI 1743 GGGCCC GGGCC^C Asp700I 1456 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 720 GGTACC G^GTACC AvaI 129 CCCGGG C^[CT]CG[GA]G BanII 1743 GGGCCC G[GA]GC[CT]^C BbeI 144 GGCGCC GGCGC^C BbsI 817 GAAGAC GAAGAC(2/6) BclI 165 TGATCA T^GATCA BfaI 832 CTAG C^TAG BlpI 974 GCTCAGC GC^T[ACGT]AGC BmyI 1743 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 974 GCTCAGC GC^T[ACGT]AGC BpuAI 817 GAAGAC GAAGAC(2/6) BsaHI 144 GGCGCC G[GA]^CG[CT]C BseRI 1207 GAGGAG GAGGAG(10/8) BsgI 1156 GTGCAG GTGCAG(16/14) BsmFI 453 GGGAC GGGAC(10/14) BsmI 349 GAATGC GAATGC(1/-1) BsoBI 129 CCCGGG C^[CT]CG[GA]G Bsp1286I 1743 GGGCCC G[AGT]GC[ACT]^C BspDI 1687 ATCGAT AT^CGAT BspHI 1383 TCATGA T^CATGA BspLU11I 594 ACATGT A^CATGT BspMI 609 GCAGGT ACCTGC(4/8) BsrDI 353 GCAATG GCAATG(2/0) BssSI 1286 CACGAG CACGAG(-5/-1) BstYI 316 GGATCT [GA]^GATC[CT] Bsu36I 61 CCTGAGG CC^T[ACGT]AGG CelII 974 GCTCAGC GC^T[ACGT]AGC ClaI 1687 ATCGAT AT^CGAT DsaI 273 CCATGG C^C[GA][CT]GG Eco81I 61 CCTGAGG CC^T[ACGT]AGG EcoRI 542 GAATTC G^AATTC EcoRV 794 GATATC GAT^ATC FbaI 165 TGATCA T^GATCA FseI 1219 GGCCGGCC GGCCGG^CC HaeII 144 GGCGCC [GA]GCGC^[CT] HgaI 435 GACGC GACGC(5/10) Hin1I 144 GGCGCC G[GA]^CG[CT]C KasI 144 GGCGCC G^GCGCC KpnI 720 GGTACC GGTAC^C MaeI 832 CTAG C^TAG MflI 316 GGATCT [GA]^GATC[CT] NarI 144 GGCGCC GG^CGCC NcoI 273 CCATGG C^CATGG PvuI 1467 CGATCG CGAT^CG RcaI 1383 TCATGA T^CATGA SfoI 144 GGCGCC GGC^GCC SgrAI 140 CGCCGGCG C[GA]^CCGG[CT]G SmaI 129 CCCGGG CCC^GGG SmlI 1015 CTTGAG C^T[CT][GA]AG SspI 649 AATATT AAT^ATT XhoII 316 GGATCT [GA]^GATC[CT] XmaCI 129 CCCGGG C^CCGGG XmaI 129 CCCGGG C^CCGGG XmnI 1456 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 594 ACATGT A^C[GA][CT]GT 1480 ACGTGT AlwNI 335 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1133 CAGGAACTG AvaII 178 GGACC G^G[AT]CC 396 GGTCC BglI 136 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 245 GCCTCCCTGGC BpmI 182 CTGGAG CTGGAG(16/14) 320 CTGGAG BsmAI 1018 GAGAC GTCTC(1/5) 266 GTCTC BsrI 153 CCAGT ACTGG(1/-1) 1245 CCAGT DraII 65 AGGCCCC [GA]G^G[ACGT]CC[CT] 1742 AGGGCCC EcoO109I 65 AGGCCCC [GA]G^G[ACGT]CC[CT] 1742 AGGGCCC EcoT14I 273 CCATGG C^C[AT][AT]GG 464 CCTTGG EcoT22I 222 ATGCAT ATGCA^T 1121 ATGCAT MslI 670 CATTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1759 CACGACAATG MspA1I 79 CAGCTG C[AC]G^C[GT]G 1720 CAGCTG NaeI 141 GCCGGC GCC^GGC 1220 GCCGGC NgoMIV 141 GCCGGC G^CCGGC 1220 GCCGGC NsiI 222 ATGCAT ATGCA^T 1121 ATGCAT PleI 565 GACTC GAGTC(4/5) 712 GACTC PstI 381 CTGCAG CTGCA^G 1717 CTGCAG PvuII 79 CAGCTG CAG^CTG 1720 CAGCTG StyI 273 CCATGG C^C[AT][AT]GG 464 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI BanI BbvI BcnI BsaJI BsiEI BsiYI BslI BsrFI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I