(Circular) (Six-base) MAPSORT of: pFN21AE1177.seq Check: 2876 from: 1 to: 6757 pFN21AE1177 6757 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4567 278 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2413 4100 1937 Size: 476 1687 4594 Fragments arranged by size: 4594 1687 476 Acc65I G'GTAC_C Cuts at: 2725 4078 2725 Size: 1353 5404 AclI AA'CG_TT Cuts at: 1563 4884 5257 1563 Size: 3321 373 3063 Fragments arranged by size: 3321 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2553 3324 4820 6204 6472 1632 Size: 921 771 1496 1384 268 1917 Fragments arranged by size: 1917 1496 1384 921 771 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6560 AflIII A'CryG_T Cuts at: 1203 2599 3485 5656 6633 1203 Size: 1396 886 2171 977 1327 Fragments arranged by size: 2171 1396 1327 977 886 AgeI A'CCGG_T Cuts at: 5588 5588 Size: 6757 AhdI GACnn_n'nnGTC Cuts at: 5486 5486 Size: 6757 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6757 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6757 AlwNI CAG_nnn'CTG Cuts at: 2345 3143 4500 6072 2345 Size: 798 1357 1572 3030 Fragments arranged by size: 3030 1572 1357 798 ApaI G_GGCC'C Cuts at: 3752 3752 Size: 6757 ApaLI G'TGCA_C Cuts at: 4814 5970 4814 Size: 1156 5601 ApoI r'AATT_y Cuts at: 1493 2474 2547 3054 4066 4292 4444 6553 1493 Size: 981 73 507 1012 226 152 2109 1697 Fragments arranged by size: 2109 1697 1012 981 507 226 152 73 AseI AT'TA_AT Cuts at: 160 1032 5311 160 Size: 872 4279 1606 Fragments arranged by size: 4279 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 AvaI C'yCGr_G Cuts at: 1870 1943 2134 4082 1870 Size: 73 191 1948 4545 Fragments arranged by size: 4545 1948 191 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2717 2750 2717 Size: 33 6724 BamHI G'GATC_C Cuts at: 4087 4454 4087 Size: 367 6390 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2149 2725 2873 3382 4078 Size: 325 217 627 362 576 148 509 696 Cuts at: 4078 5533 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 696 627 576 509 362 325 217 148 BanII G_rGCy'C Cuts at: 1391 3752 4076 1391 Size: 2361 324 4072 Fragments arranged by size: 4072 2361 324 BbeI G_GCGC'C Cuts at: 2153 2153 Size: 6757 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 928 Size: 971 930 4856 Fragments arranged by size: 4856 971 930 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4948 4982 1903 Size: 34 3011 34 3678 Fragments arranged by size: 3678 3011 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4649 5859 4649 Size: 1210 5547 BclI T'GATC_A Cuts at: 1548 2170 1548 Size: 622 6135 BfrBI ATG'CAT Cuts at: 2229 3128 2229 Size: 899 5858 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 2256 5368 Size: 107 122 71 631 605 475 109 3112 Cuts at: 5368 136 Size: 1525 Fragments arranged by size: 3112 1525 631 605 475 122 109 107 71 BglII A'GATC_T Cuts at: 6752 6752 Size: 6757 BlpI GC'TnA_GC Cuts at: 2980 4174 2980 Size: 1194 5563 Bme1580I G_kGCm'C Cuts at: 1790 3752 4818 5974 1790 Size: 1962 1066 1156 2573 Fragments arranged by size: 2573 1962 1156 1066 BmgBI CAC'GTC Cuts at: 1318 3486 3880 1318 Size: 2168 394 4195 Fragments arranged by size: 4195 2168 394 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4488 5446 442 Size: 416 53 83 399 24 3071 958 1753 Fragments arranged by size: 3071 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6757 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2208 2346 5417 1239 Size: 969 138 3071 2579 Fragments arranged by size: 3071 2579 969 138 Bpu10I CC'TnA_GC Cuts at: 3684 3684 Size: 6757 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3041 4882 5747 6045 3041 Size: 1841 865 298 3753 Fragments arranged by size: 3753 1841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5420 882 Size: 617 3921 2219 Fragments arranged by size: 3921 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4548 493 Size: 711 3344 2702 Fragments arranged by size: 3344 2702 711 BsaBI GATnn'nnATC Cuts at: 6751 6751 Size: 6757 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2150 4564 4946 5581 Size: 53 83 186 936 617 2414 382 635 Cuts at: 5581 275 Size: 1451 Fragments arranged by size: 2414 1451 936 635 617 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5190 5588 5862 6009 6420 5190 Size: 398 274 147 411 5527 Fragments arranged by size: 5527 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6532 Fragments arranged by size: 6532 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3227 1186 Size: 2041 4716 BseYI C'CCAG_C Cuts at: 2454 2966 5960 6456 2454 Size: 512 2994 496 2755 Fragments arranged by size: 2994 2755 512 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3182 1163 Size: 573 1446 4738 Fragments arranged by size: 4738 1446 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3333 3475 3779 4207 4968 5996 Size: 275 186 1332 142 304 428 761 1028 Cuts at: 5996 1540 Size: 2301 Fragments arranged by size: 2301 1332 1028 761 428 304 275 186 142 BsiHKAI G_wGCw'C Cuts at: 4818 4903 5974 4818 Size: 85 1071 5601 Fragments arranged by size: 5601 1071 85 BsmI GAATG_Cn' Cuts at: 2360 4268 4361 2360 Size: 1908 93 4756 Fragments arranged by size: 4756 1908 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3752 4076 4818 4903 5974 1391 Size: 399 1962 324 742 85 1071 2174 Fragments arranged by size: 2174 1962 1071 742 399 324 85 BspEI T'CCGG_A Cuts at: 6420 6420 Size: 6757 BspHI T'CATG_A Cuts at: 3388 4646 6376 3388 Size: 1258 1730 3769 Fragments arranged by size: 3769 1730 1258 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2605 4112 844 Size: 1761 1507 3489 Fragments arranged by size: 3489 1761 1507 BsrBI CCG'CTC Cuts at: 1394 1823 4644 1394 Size: 429 2821 3507 Fragments arranged by size: 3507 2821 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2365 5252 5426 65 Size: 1183 1117 2887 174 1396 Fragments arranged by size: 2887 1396 1183 1117 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2116 2146 3225 3516 5401 5588 1700 Size: 110 306 30 1079 291 1885 187 2869 Fragments arranged by size: 2869 1885 1079 306 291 187 110 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6757 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6757 BssSI C'ACGA_G Cuts at: 3291 4817 5829 3291 Size: 1526 1012 4219 Fragments arranged by size: 4219 1526 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2744 3721 3944 794 Size: 1950 977 223 3607 Fragments arranged by size: 3607 1950 977 223 BstBI TT'CG_AA Cuts at: 5561 5561 Size: 6757 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6757 BstYI r'GATC_y Cuts at: 1972 2321 4087 4454 4839 4856 6308 6394 6406 Size: 349 1766 367 385 17 1452 86 12 Cuts at: 6406 6752 1972 Size: 346 1977 Fragments arranged by size: 1977 1766 1452 385 367 349 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2067 2067 Size: 6757 BtgI C'CryG_G Cuts at: 513 1065 2278 513 Size: 552 1213 4992 Fragments arranged by size: 4992 1213 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6018 Fragments arranged by size: 6018 683 56 BtsI GCAGTG_nn' Cuts at: 2383 3170 4273 5067 5087 2383 Size: 787 1103 794 20 4053 Fragments arranged by size: 4053 1103 794 787 20 ClaI AT'CG_AT Cuts at: 1554 3693 1554 Size: 2139 4618 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6722 DraI TTT'AAA Cuts at: 4061 4061 Size: 6757 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5764 809 Size: 507 4448 1802 Fragments arranged by size: 4448 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2101 3223 3227 Size: 54 1425 50 275 18 271 1122 4 Cuts at: 3227 4204 5093 6722 8 Size: 977 889 1629 43 Fragments arranged by size: 1629 1425 1122 977 889 275 271 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 4204 1537 Size: 275 2392 4090 Fragments arranged by size: 4090 2392 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4687 1353 Size: 3334 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2063 2264 5344 5718 5864 387 Size: 1064 138 474 201 3080 374 146 1280 Fragments arranged by size: 3080 1280 1064 474 374 201 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 2346 2553 3324 4820 5417 6204 Size: 393 576 138 207 771 1496 597 787 Cuts at: 6204 6472 1239 Size: 268 1524 Fragments arranged by size: 1524 1496 787 771 597 576 393 268 207 138 EcoO109I rG'GnC_Cy Cuts at: 2071 3748 2071 Size: 1677 5080 EcoRI G'AATT_C Cuts at: 2547 4066 4444 2547 Size: 1519 378 4860 Fragments arranged by size: 4860 1519 378 EcoRV GAT'ATC Cuts at: 2801 2801 Size: 6757 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2877 3595 2877 Size: 718 6039 FseI GG_CCGG'CC Cuts at: 3229 3229 Size: 6757 FspI TGC'GCA Cuts at: 5263 5263 Size: 6757 HaeII r_GCGC'y Cuts at: 1678 2153 5904 1678 Size: 475 3751 2531 Fragments arranged by size: 3751 2531 475 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2306 2317 2338 3249 Size: 32 135 32 713 21 11 21 911 Cuts at: 3249 3281 3388 3420 5398 5430 5472 5504 1373 Size: 32 107 32 1978 32 42 32 2626 Fragments arranged by size: 2626 1978 911 713 135 107 42 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2635 3241 3319 3739 4101 4347 Size: 988 281 697 606 78 420 362 246 Cuts at: 4347 669 Size: 3079 Fragments arranged by size: 3079 988 697 606 420 362 281 246 78 HindIII A'AGCT_T Cuts at: 748 748 Size: 6757 HpaI GTT'AAC Cuts at: 4347 4347 Size: 6757 KasI G'GCGC_C Cuts at: 2149 2149 Size: 6757 KpnI G_GTAC'C Cuts at: 2729 4082 2729 Size: 1353 5404 MfeI C'AATT_G Cuts at: 4356 4356 Size: 6757 MluI A'CGCG_T Cuts at: 6633 6633 Size: 6757 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2161 2592 3519 5871 6055 1305 Size: 856 431 927 2352 184 2007 Fragments arranged by size: 2352 2007 927 856 431 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4935 Fragments arranged by size: 4935 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2679 3768 4715 5074 5233 518 Size: 648 1513 1089 947 359 159 2042 Fragments arranged by size: 2042 1513 1089 947 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2086 3727 4173 4850 5998 6243 1606 Size: 480 1641 446 677 1148 245 2120 Fragments arranged by size: 2120 1641 1148 677 480 446 245 NaeI GCC'GGC Cuts at: 2148 3227 2148 Size: 1079 5678 NarI GG'CG_CC Cuts at: 2150 2150 Size: 6757 NcoI C'CATG_G Cuts at: 513 1065 2278 513 Size: 552 1213 4992 Fragments arranged by size: 4992 1213 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6757 NgoMIV G'CCGG_C Cuts at: 2146 3225 2146 Size: 1079 5678 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6757 NotI GC'GGCC_GC Cuts at: 4204 4204 Size: 6757 NsiI A_TGCA'T Cuts at: 2231 3130 2231 Size: 899 5858 NspI r_CATG'y Cuts at: 1132 1229 2603 2974 3785 4115 5660 1132 Size: 97 1374 371 811 330 1545 2229 Fragments arranged by size: 2229 1545 1374 811 371 330 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6757 PciI A'CATG_T Cuts at: 2599 5656 2599 Size: 3057 3700 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6757 PfoI T'CCnGG_A Cuts at: 2221 2221 Size: 6757 PmeI GTTT'AAAC Cuts at: 4061 4061 Size: 6757 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3453 3485 4826 4858 6339 6371 3453 Size: 32 1341 32 1481 32 3839 Fragments arranged by size: 3839 1481 1341 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6757 PsiI TTA'TAA Cuts at: 2607 4327 2607 Size: 1720 5037 PspOMI G'GGCC_C Cuts at: 3748 3748 Size: 6757 PstI C_TGCA'G Cuts at: 830 2390 3726 4109 830 Size: 1560 1336 383 3478 Fragments arranged by size: 3478 1560 1336 383 PvuI CG_AT'CG Cuts at: 2001 3475 2001 Size: 1474 5283 PvuII CAG'CTG Cuts at: 2086 3727 2086 Size: 1641 5116 SalI G'TCGA_C Cuts at: 1936 4099 1936 Size: 2163 4594 SbfI CC_TGCA'GG Cuts at: 4109 4109 Size: 6757 ScaI AGT'ACT Cuts at: 5005 6717 5005 Size: 1712 5045 SfcI C'TryA_G Cuts at: 826 1045 2386 2414 3637 3722 3990 4105 5240 Size: 219 1341 28 1223 85 268 115 1135 Cuts at: 5240 5921 6112 826 Size: 681 191 1471 Fragments arranged by size: 1471 1341 1223 1135 681 268 219 191 115 85 28 SfoI GGC'GCC Cuts at: 2151 2151 Size: 6757 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 SgrAI Cr'CCGG_yG Cuts at: 1700 2146 1700 Size: 446 6311 SmaI CCC'GGG Cuts at: 1872 2136 4084 1872 Size: 264 1948 4545 Fragments arranged by size: 4545 1948 264 SmlI C'TyrA_G Cuts at: 820 1017 1943 3020 4861 5762 6024 820 Size: 197 926 1077 1841 901 262 1553 Fragments arranged by size: 1841 1553 1077 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4548 493 Size: 4055 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6757 SphI G_CATG'C Cuts at: 1132 4115 1132 Size: 2983 3774 SspI AAT'ATT Cuts at: 5 52 2656 4681 5 Size: 47 2604 2025 2081 Fragments arranged by size: 2604 2081 2025 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6757 StyI C'CwwG_G Cuts at: 513 1065 2278 2469 4196 513 Size: 552 1213 191 1727 3074 Fragments arranged by size: 3074 1727 1213 552 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3370 3600 4784 4801 4954 5139 5584 Size: 1255 1790 230 1184 17 153 185 445 Cuts at: 5584 325 Size: 1498 Fragments arranged by size: 1790 1498 1255 1184 445 230 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2160 2885 2916 Size: 274 80 33 51 1443 183 725 31 Cuts at: 2916 5003 6715 96 Size: 2087 1712 138 Fragments arranged by size: 2087 1712 1443 725 274 183 138 80 51 33 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2081 2113 6717 6749 2081 Size: 32 4604 32 2089 Fragments arranged by size: 4604 2089 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6757 XbaI T'CTAG_A Cuts at: 4093 4093 Size: 6757 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6757 XmaI C'CCGG_G Cuts at: 1870 2134 4082 1870 Size: 264 1948 4545 Fragments arranged by size: 4545 1948 264 XmnI GAAnn'nnTTC Cuts at: 3465 4886 3465 Size: 1421 5336 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4565 276 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FspAI NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI