(Linear) (Six-base) MAPSORT of: KIEE1177.seq Check: 8200 from: 1 to: 2052 KIEE1177.orf With 182 enzymes: SgfI * March 2, 2010 14:32 .. AccI GT'mk_AC Cuts at: 0 408 2052 Size: 408 1644 Acc65I G'GTAC_C Cuts at: 0 720 2052 Size: 720 1332 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 548 1319 2052 Size: 548 771 733 Fragments arranged by size: 771 733 548 AflIII A'CryG_T Cuts at: 0 594 1480 2052 Size: 594 886 572 Fragments arranged by size: 886 594 572 AlwNI CAG_nnn'CTG Cuts at: 0 340 1138 2052 Size: 340 798 914 Fragments arranged by size: 914 798 340 ApaI G_GGCC'C Cuts at: 0 1747 2052 Size: 1747 305 ApoI r'AATT_y Cuts at: 0 469 542 1049 2052 Size: 469 73 507 1003 Fragments arranged by size: 1003 507 469 73 AvaI C'yCGr_G Cuts at: 0 129 2052 Size: 129 1923 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 712 745 2052 Size: 712 33 1307 Fragments arranged by size: 1307 712 33 BanI G'GyrC_C Cuts at: 0 144 720 868 1377 2052 Size: 144 576 148 509 675 Fragments arranged by size: 675 576 509 148 144 BanII G_rGCy'C Cuts at: 0 1747 2052 Size: 1747 305 BbeI G_GCGC'C Cuts at: 0 148 2052 Size: 148 1904 BbsI GAAGACnn'nnnn_ Cuts at: 0 824 2052 Size: 824 1228 BclI T'GATC_A Cuts at: 0 165 2052 Size: 165 1887 BfrBI ATG'CAT Cuts at: 0 224 1123 2052 Size: 224 899 929 Fragments arranged by size: 929 899 224 BglI GCCn_nnn'nGGC Cuts at: 0 142 251 2052 Size: 142 109 1801 Fragments arranged by size: 1801 142 109 BlpI GC'TnA_GC Cuts at: 0 975 2052 Size: 975 1077 Bme1580I G_kGCm'C Cuts at: 0 1747 2052 Size: 1747 305 BmgBI CAC'GTC Cuts at: 0 1481 1875 2052 Size: 1481 394 177 Fragments arranged by size: 1481 394 177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 203 341 2052 Size: 203 138 1711 Fragments arranged by size: 1711 203 138 Bpu10I CC'TnA_GC Cuts at: 0 1679 2052 Size: 1679 373 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1036 2052 Size: 1036 1016 BsaHI Gr'CG_yC Cuts at: 0 145 2052 Size: 145 1907 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1222 2052 Size: 1222 830 BseYI C'CCAG_C Cuts at: 0 449 961 2052 Size: 449 512 1091 Fragments arranged by size: 1091 512 449 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1177 2052 Size: 1177 875 BsiEI CG_ry'CG Cuts at: 0 1328 1470 1774 2052 Size: 1328 142 304 278 Fragments arranged by size: 1328 304 278 142 BsmI GAATG_Cn' Cuts at: 0 355 2052 Size: 355 1697 Bsp1286I G_dGCh'C Cuts at: 0 1747 2052 Size: 1747 305 BspHI T'CATG_A Cuts at: 0 1383 2052 Size: 1383 669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 600 2052 Size: 600 1452 BsrDI GCAATG_nn' Cuts at: 0 360 2052 Size: 360 1692 BsrFI r'CCGG_y Cuts at: 0 111 141 1220 1511 2052 Size: 111 30 1079 291 541 Fragments arranged by size: 1079 541 291 111 30 BssSI C'ACGA_G Cuts at: 0 1286 2052 Size: 1286 766 BstAPI GCAn_nnn'nTGC Cuts at: 0 739 1716 1939 2052 Size: 739 977 223 113 Fragments arranged by size: 977 739 223 113 BstYI r'GATC_y Cuts at: 0 316 2052 Size: 316 1736 Bsu36I CC'TnA_GG Cuts at: 0 62 2052 Size: 62 1990 BtgI C'CryG_G Cuts at: 0 273 2052 Size: 273 1779 BtsI GCAGTG_nn' Cuts at: 0 378 1165 2052 Size: 378 787 887 Fragments arranged by size: 887 787 378 ClaI AT'CG_AT Cuts at: 0 1688 2052 Size: 1688 364 EaeI y'GGCC_r Cuts at: 0 96 1218 1222 2052 Size: 96 1122 4 830 Fragments arranged by size: 1122 830 96 4 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 58 259 2052 Size: 58 201 1793 Fragments arranged by size: 1793 201 58 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 203 341 548 1319 2052 Size: 203 138 207 771 733 Fragments arranged by size: 771 733 207 203 138 EcoO109I rG'GnC_Cy Cuts at: 0 66 1743 2052 Size: 66 1677 309 Fragments arranged by size: 1677 309 66 EcoRI G'AATT_C Cuts at: 0 542 2052 Size: 542 1510 EcoRV GAT'ATC Cuts at: 0 796 2052 Size: 796 1256 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 872 1590 2052 Size: 872 718 462 Fragments arranged by size: 872 718 462 FseI GG_CCGG'CC Cuts at: 0 1224 2052 Size: 1224 828 HaeII r_GCGC'y Cuts at: 0 148 2052 Size: 148 1904 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 280 301 312 333 1244 1276 1383 1415 Size: 280 21 11 21 911 32 107 32 Cuts at: 1415 2052 Size: 637 Fragments arranged by size: 911 637 280 107 32 32 21 21 11 HincII GTy'rAC Cuts at: 0 630 1236 1314 1734 2052 Size: 630 606 78 420 318 Fragments arranged by size: 630 606 420 318 78 KasI G'GCGC_C Cuts at: 0 144 2052 Size: 144 1908 KpnI G_GTAC'C Cuts at: 0 724 2052 Size: 724 1328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 156 587 1514 2052 Size: 156 431 927 538 Fragments arranged by size: 927 538 431 156 MslI CAynn'nnrTG Cuts at: 0 674 1763 2052 Size: 674 1089 289 Fragments arranged by size: 1089 674 289 MspA1I CmG'CkG Cuts at: 0 81 1722 2052 Size: 81 1641 330 Fragments arranged by size: 1641 330 81 NaeI GCC'GGC Cuts at: 0 143 1222 2052 Size: 143 1079 830 Fragments arranged by size: 1079 830 143 NarI GG'CG_CC Cuts at: 0 145 2052 Size: 145 1907 NcoI C'CATG_G Cuts at: 0 273 2052 Size: 273 1779 NgoMIV G'CCGG_C Cuts at: 0 141 1220 2052 Size: 141 1079 832 Fragments arranged by size: 1079 832 141 NsiI A_TGCA'T Cuts at: 0 226 1125 2052 Size: 226 899 927 Fragments arranged by size: 927 899 226 NspI r_CATG'y Cuts at: 0 598 969 1780 2052 Size: 598 371 811 272 Fragments arranged by size: 811 598 371 272 PciI A'CATG_T Cuts at: 0 594 2052 Size: 594 1458 PfoI T'CCnGG_A Cuts at: 0 216 2052 Size: 216 1836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1448 1480 2052 Size: 1448 32 572 Fragments arranged by size: 1448 572 32 PsiI TTA'TAA Cuts at: 0 602 2052 Size: 602 1450 PspOMI G'GGCC_C Cuts at: 0 1743 2052 Size: 1743 309 PstI C_TGCA'G Cuts at: 0 385 1721 2052 Size: 385 1336 331 Fragments arranged by size: 1336 385 331 PvuI CG_AT'CG Cuts at: 0 1470 2052 Size: 1470 582 PvuII CAG'CTG Cuts at: 0 81 1722 2052 Size: 81 1641 330 Fragments arranged by size: 1641 330 81 SfcI C'TryA_G Cuts at: 0 381 409 1632 1717 1985 2052 Size: 381 28 1223 85 268 67 Fragments arranged by size: 1223 381 268 85 67 28 SfoI GGC'GCC Cuts at: 0 146 2052 Size: 146 1906 SgrAI Cr'CCGG_yG Cuts at: 0 141 2052 Size: 141 1911 SmaI CCC'GGG Cuts at: 0 131 2052 Size: 131 1921 SmlI C'TyrA_G Cuts at: 0 1015 2052 Size: 1015 1037 SspI AAT'ATT Cuts at: 0 651 2052 Size: 651 1401 StyI C'CwwG_G Cuts at: 0 273 464 2052 Size: 273 191 1588 Fragments arranged by size: 1588 273 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1365 1595 2052 Size: 1365 230 457 Fragments arranged by size: 1365 457 230 TatI w'GTAC_w Cuts at: 0 155 880 911 2052 Size: 155 725 31 1141 Fragments arranged by size: 1141 725 155 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 76 108 2052 Size: 76 32 1944 Fragments arranged by size: 1944 76 32 XmaI C'CCGG_G Cuts at: 0 129 2052 Size: 129 1923 XmnI GAAnn'nnTTC Cuts at: 0 1460 2052 Size: 1460 592 Enzymes that do cut: AccI Acc65I AcuI AflIII AlwNI ApaI ApoI AvaI BaeI BanI BanII BbeI BbsI BclI BfrBI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtsI ClaI EaeI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FseI HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI PpiI PsiI PspOMI PstI PvuI PvuII SfcI SfoI SgrAI SmaI SmlI SspI StyI TaqII TatI TstI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI BbvCI BcgI BciVI BglII BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgZI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI FspI FspAI HindIII HpaI MfeI MluI MscI NdeI NheI NotI NruI PacI PasI PflMI PmeI PmlI PpuMI PshAI PspXI PsrI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI ZraI