Restriction map for
pFN21AB7653
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspHI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr13I ClaI CpoI DraII DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HincII HindII HpaI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MscI MslI
MunI MvnI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
Sau96I SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 887 TCCGGA T^CCGGA
AcyI 13 GGCGCC G[GA]^CG[CT]C
Asp700I 453 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 13 GGCGCC G^G[CT][GA]CC
BbeI 13 GGCGCC GGCGC^C
BbsI 862 GTCTTC GAAGAC(2/6)
BbvCI 19 GCTGAGG CCTCAGC(-5/-2)
BcnI 621 CCGGG CC^[GC]GG
BglII 975 AGATCT A^GATCT
BpuAI 862 GTCTTC GAAGAC(2/6)
BsaHI 13 GGCGCC G[GA]^CG[CT]C
BsaJI 959 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 887 TCCGGA [AT]^CCGG[AT]
BseAI 887 TCCGGA T^CCGGA
BsgI 262 GTGCAG GTGCAG(16/14)
Bsp1407I 358 TGTACA T^GTACA
BspEI 887 TCCGGA T^CCGGA
BsrDI 919 GCAATG GCAATG(2/0)
BsrFI 32 GCCGGC [GA]^CCGG[CT]
BsrGI 358 TGTACA T^GTACA
BstAPI 333 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 14 GCGC GCG^C
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
DraI 241 TTTAAA TTT^AAA
DrdI 505 GACGGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 302 CTCTTC CTCTTC(1/4)
EcoNI 339 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 601 GAATTC G^AATTC
HaeII 13 GGCGCC [GA]GCGC^[CT]
HhaI 14 GCGC GCG^C
Hin1I 13 GGCGCC G[GA]^CG[CT]C
HinP1I 14 GCGC G^CGC
HindIII 446 AAGCTT A^AGCTT
HphI 508 GGTGA GGTGA(8/7)
KasI 13 GGCGCC G^GCGCC
Ksp632I 302 CTCTTC CTCTTC(1/4)
MaeIII 568 GTCAC ^GT[ACGT]AC
MroI 887 TCCGGA T^CCGGA
NaeI 32 GCCGGC GCC^GGC
NarI 13 GGCGCC GG^CGCC
NciI 621 CCGGG CC^[GC]GG
NgoMIV 32 GCCGGC G^CCGGC
NspI 589 GCATGT [GA]CATG^[CT]
PleI 756 GAGTC GAGTC(4/5)
PstI 340 CTGCAG CTGCA^G
PvuII 217 CAGCTG CAG^CTG
SapI 301 GCTCTTC GCTCTTC(1/4)
SfoI 13 GGCGCC GGC^GCC
SspBI 358 TGTACA T^GTACA
StuI 26 AGGCCT AGG^CCT
TaqI 306 TCGA T^CGA
Tsp45I 568 GTCAC ^GT[GC]AC
TthHB8I 306 TCGA T^CGA
XmnI 453 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 55 GAATTT [GA]^AATT[CT]
601 GAATTC
AlwI 796 GATCC GGATC(4/5)
78 GGATC
AlwNI 517 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
646 CAGCTTCTG
ApoI 55 GAATTT [GA]^AATT[CT]
601 GAATTC
BanII 891 GAGCTC G[GA]GC[CT]^C
964 GAGCTC
BseRI 22 GAGGAG GAGGAG(10/8)
437 GAGGAG
BsmAI 656 GAGAC GTCTC(1/5)
400 GTCTC
BsmFI 116 GGGAC GGGAC(10/14)
705 GTCCC
BstYI 78 GGATCT [GA]^GATC[CT]
975 AGATCT
MflI 78 GGATCT [GA]^GATC[CT]
975 AGATCT
MspA1I 17 CCGCTG C[AC]G^C[GT]G
217 CAGCTG
NlaIV 13 GGCGCC GG[ACGT]^[ACGT]CC
852 GGAACC
PflMI 516 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
837 CCAGTCTGTGG
SacI 891 GAGCTC GAGCT^C
964 GAGCTC
SfcI 340 CTGCAG C^T[GA][CT]AG
584 CTATAG
TfiI 526 GATTC G^A[AT]TC
764 GATTC
Van91I 516 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
837 CCAGTCTGTGG
XhoII 78 GGATCT [GA]^GATC[CT]
975 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BpmI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
Tru9I TseI Tsp509I TspRI