Restriction map for
pFN21AB7653
(Circular) (Six-base) MAPSORT of: pFN21AB7653.seq Check: 9873 from: 1 to: 5707
pFN21AB7653 5707 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2883 3770 5154 5422 1632
Size: 492 759 887 1384 268 1917
Fragments arranged by size:
1917 1384 887 759 492 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2833 1184
Size: 1649 4058
AlwNI CAG_nnn'CTG
Cuts at: 2527 2656 3450 5022 2527
Size: 129 794 1572 3212
Fragments arranged by size:
3212 1572 794 129
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2060 2606 3016 3242 3394 5503 1493
Size: 567 546 410 226 152 2109 1697
Fragments arranged by size:
2109 1697 567 546 410 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 3032 1870
Size: 73 1089 4545
Fragments arranged by size:
4545 1089 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 3028 4483 618
Size: 325 217 627 231 1010 1455 1842
Fragments arranged by size:
1842 1455 1010 627 325 231 217
BanII G_rGCy'C
Cuts at: 1391 2900 2973 3026 1391
Size: 1509 73 53 4072
Fragments arranged by size:
4072 1509 73 53
BbeI G_GCGC'C
Cuts at: 2022 2022
Size: 5707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2860 928
Size: 971 961 3775
Fragments arranged by size:
3775 971 961
BbvCI CC'TCA_GC
Cuts at: 2025 2025
Size: 5707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2980 5702 2980
Size: 2722 2985
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 3768 4924 1790
Size: 1978 1156 2573
Fragments arranged by size:
2573 1978 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 2392 3014 4367 1239
Size: 938 215 622 1353 2579
Fragments arranged by size:
2579 1353 938 622 215
Bpu10I CC'TnA_GC
Cuts at: 2025 2025
Size: 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4370 882
Size: 617 2871 2219
Fragments arranged by size:
2871 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 3514 3896 4531
Size: 53 83 186 936 486 1495 382 635
Cuts at: 4531 275
Size: 1451
Fragments arranged by size:
1495 1451 936 635 486 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2892 4140 4538 4812 4959 5370 2892
Size: 1248 398 274 147 411 3229
Fragments arranged by size:
3229 1248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2457 1186
Size: 856 415 4436
Fragments arranged by size:
4436 856 415
BseYI C'CCAG_C
Cuts at: 4910 5406 4910
Size: 496 5211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2288 1163
Size: 573 552 4582
Fragments arranged by size:
4582 573 552
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 2634 2900 2973 3768 3853 4924 2244
Size: 390 266 73 795 85 1071 3027
Fragments arranged by size:
3027 1071 795 390 266 85 73
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2634 2900 2973 3026 3768 3853
Size: 399 454 390 266 73 53 742 85
Cuts at: 3853 4924 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 390 266 85
73 53
BspEI T'CCGG_A
Cuts at: 2892 5370 2892
Size: 2478 3229
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 844
Size: 2218 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2931 4202 4376 65
Size: 1183 1683 1271 174 1396
Fragments arranged by size:
1683 1396 1271 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 4351 4538 1700
Size: 110 227 2314 187 2869
Fragments arranged by size:
2869 2314 227 187 110
BsrGI T'GTAC_A
Cuts at: 96 2363 96
Size: 2267 3440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2344 794
Size: 1550 4157
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2083 2980 3037 3404 3789 3806 5258 5344
Size: 111 897 57 367 385 17 1452 86
Cuts at: 5344 5356 5702 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 897 385 367 346 111 86
57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 2419 3223 4017 4037 2419
Size: 804 794 20 4089
Fragments arranged by size:
4089 804 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 2248 3011 2248
Size: 763 4944
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2516 4714 809
Size: 507 1200 2198 1802
Fragments arranged by size:
2198 1802 1200 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3154 4043 5672
Size: 54 1425 50 275 18 1324 889 1629
Cuts at: 5672 8
Size: 43
Fragments arranged by size:
1629 1425 1324 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 3637 1353
Size: 960 1324 3423
Fragments arranged by size:
3423 1324 960
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2898 2971 2898
Size: 73 5634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2177 2392 2883 3014 3770 4367
Size: 393 492 53 215 491 131 756 597
Cuts at: 4367 5154 5422 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 756 597 492 491 393 268
215 131 53
EcoNI CCTnn'n_nnAGG
Cuts at: 2348 2348
Size: 5707
EcoRI G'AATT_C
Cuts at: 2606 3016 3394 2606
Size: 410 378 4919
Fragments arranged by size:
4919 410 378
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 2022 4854 1678
Size: 344 2832 2531
Fragments arranged by size:
2832 2531 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373
Size: 32 135 32 2776 32 42 32 2626
Fragments arranged by size:
2776 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2451 748
Size: 1703 4004
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KasI G'GCGC_C
Cuts at: 2018 2018
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4821 5005 1305
Size: 3516 184 2007
Fragments arranged by size:
3516 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3885
Fragments arranged by size:
3885 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3665 4024 4183 518
Size: 648 2499 359 159 2042
Fragments arranged by size:
2499 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2224 3123 3800 4948 5193 1606
Size: 418 200 899 677 1148 245 2120
Fragments arranged by size:
2120 1148 899 677 418 245 200
NaeI GCC'GGC
Cuts at: 2039 2039
Size: 5707
NarI GG'CG_CC
Cuts at: 2019 2019
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NgoMIV G'CCGG_C
Cuts at: 2037 2037
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 2598 3065 4610 1132
Size: 97 1369 467 1545 2229
Fragments arranged by size:
2229 1545 1369 467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2527 2848 1108
Size: 1419 321 3967
Fragments arranged by size:
3967 1419 321
PfoI T'CCnGG_A
Cuts at: 2520 2764 2973 2520
Size: 244 209 5254
Fragments arranged by size:
5254 244 209
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 2349 3059 830
Size: 1519 710 3478
Fragments arranged by size:
3478 1519 710
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2224 2224
Size: 5707
SacI G_AGCT'C
Cuts at: 2900 2973 2900
Size: 73 5634
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SapI GCTCTTCn'nnn_
Cuts at: 2313 2313
Size: 5707
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 2345 2589 3055 4190 4871 5062 826
Size: 219 1300 244 466 1135 681 191 1471
Fragments arranged by size:
1471 1300 1135 681 466 244 219 191
SfoI GGC'GCC
Cuts at: 2020 2020
Size: 5707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 2033 1517
Size: 516 5191
StyI C'CwwG_G
Cuts at: 513 1065 2004 3146 513
Size: 552 939 1142 3074
Fragments arranged by size:
3074 1142 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3734 3751 3904 4089 4534 325
Size: 1255 2154 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2363 2827 3953
Size: 274 80 33 51 1443 386 464 1126
Cuts at: 3953 5665 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1126 464 386 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 2462 3836 2462
Size: 1374 4333
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI XcmI