(Circular) (Six-base) MAPSORT of: pFN21AB7821.seq Check: 3579 from: 1 to: 6313 pFN21AB7821 6313 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 3656 1937 Size: 1401 318 4594 Fragments arranged by size: 4594 1401 318 Acc65I G'GTAC_C Cuts at: 3634 3634 Size: 6313 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2724 3546 3552 4376 5760 6028 1632 Size: 1092 822 6 824 1384 268 1917 Fragments arranged by size: 1917 1384 1092 824 822 268 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 5212 6189 1203 Size: 4009 977 1327 Fragments arranged by size: 4009 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 5042 5042 Size: 6313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2151 1184 Size: 967 5346 AlwNI CAG_nnn'CTG Cuts at: 4056 5628 4056 Size: 1572 4741 ApaLI G'TGCA_C Cuts at: 4370 5526 4370 Size: 1156 5157 ApoI r'AATT_y Cuts at: 1493 2145 2273 2364 3622 3848 4000 6109 1493 Size: 652 128 91 1258 226 152 2109 1697 Fragments arranged by size: 2109 1697 1258 652 226 152 128 91 AseI AT'TA_AT Cuts at: 160 1032 4867 160 Size: 872 3835 1606 Fragments arranged by size: 3835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 3638 1870 Size: 73 1695 4545 Fragments arranged by size: 4545 1695 73 BamHI G'GATC_C Cuts at: 3643 4010 3643 Size: 367 5946 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3634 5089 618 Size: 325 217 627 1847 1455 1842 Fragments arranged by size: 1847 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2626 3632 1391 Size: 1235 1006 4072 Fragments arranged by size: 4072 1235 1006 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3151 928 Size: 971 1252 4090 Fragments arranged by size: 4090 1252 971 BbvCI CC'TCA_GC Cuts at: 2036 2036 Size: 6313 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4504 4538 1903 Size: 34 2567 34 3678 Fragments arranged by size: 3678 2567 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4205 5415 4205 Size: 1210 5103 BclI T'GATC_A Cuts at: 1548 1548 Size: 6313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 4924 136 Size: 107 122 71 631 605 428 2824 1525 Fragments arranged by size: 2824 1525 631 605 428 122 107 71 BglII A'GATC_T Cuts at: 3166 6308 3166 Size: 3142 3171 BlpI GC'TnA_GC Cuts at: 3730 3730 Size: 6313 Bme1580I G_kGCm'C Cuts at: 1790 4374 5530 1790 Size: 2584 1156 2573 Fragments arranged by size: 2584 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2783 4044 5002 Size: 416 53 83 399 24 1366 1261 958 Cuts at: 5002 442 Size: 1753 Fragments arranged by size: 1753 1366 1261 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2110 3533 4973 1239 Size: 871 1423 1440 2579 Fragments arranged by size: 2579 1440 1423 871 Bpu10I CC'TnA_GC Cuts at: 2036 2036 Size: 6313 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4438 5303 5601 4438 Size: 865 298 5150 Fragments arranged by size: 5150 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4976 882 Size: 617 3477 2219 Fragments arranged by size: 3477 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4104 493 Size: 711 2900 2702 Fragments arranged by size: 2900 2702 711 BsaBI GATnn'nnATC Cuts at: 2673 6307 2673 Size: 3634 2679 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4120 4502 5137 275 Size: 53 83 186 936 2587 382 635 1451 Fragments arranged by size: 2587 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4746 5144 5418 5565 5976 4746 Size: 398 274 147 411 5083 Fragments arranged by size: 5083 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6088 Fragments arranged by size: 6088 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6313 BseYI C'CCAG_C Cuts at: 5516 6012 5516 Size: 496 5817 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3218 3561 1163 Size: 573 1482 343 3915 Fragments arranged by size: 3915 1482 573 343 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2404 4374 4459 5530 2404 Size: 1970 85 1071 3187 Fragments arranged by size: 3187 1970 1071 85 BsmI GAATG_Cn' Cuts at: 3359 3824 3917 3359 Size: 465 93 5755 Fragments arranged by size: 5755 465 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2404 2626 3632 4374 4459 5530 1391 Size: 399 614 222 1006 742 85 1071 2174 Fragments arranged by size: 2174 1071 1006 742 614 399 222 85 BspEI T'CCGG_A Cuts at: 5976 5976 Size: 6313 BspHI T'CATG_A Cuts at: 4202 5932 4202 Size: 1730 4583 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3668 844 Size: 2824 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4200 1394 Size: 429 2377 3507 Fragments arranged by size: 3507 2377 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4808 4982 65 Size: 1183 3560 174 1396 Fragments arranged by size: 3560 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2026 4957 5144 1700 Size: 110 216 2931 187 2869 Fragments arranged by size: 2931 2869 216 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 4373 5385 4373 Size: 1012 5301 BstAPI GCAn_nnn'nTGC Cuts at: 794 2507 794 Size: 1713 4600 BstBI TT'CG_AA Cuts at: 2018 2530 5117 2018 Size: 512 2587 3214 Fragments arranged by size: 3214 2587 512 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstXI CCAn_nnnn'nTGG Cuts at: 2993 2993 Size: 6313 BstYI r'GATC_y Cuts at: 1972 3019 3166 3643 4010 4395 4412 5864 5950 Size: 1047 147 477 367 385 17 1452 86 Cuts at: 5950 5962 6308 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1047 477 385 367 346 147 86 17 12 BstZ17I GTA'TAC Cuts at: 3339 3339 Size: 6313 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5761 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 2956 3829 4623 4643 2956 Size: 873 794 20 4626 Fragments arranged by size: 4626 873 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6278 DraI TTT'AAA Cuts at: 2586 2712 3617 2586 Size: 126 905 5282 Fragments arranged by size: 5282 905 126 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5320 809 Size: 507 4004 1802 Fragments arranged by size: 4004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2101 3760 4649 Size: 54 1425 50 275 18 271 1659 889 Cuts at: 4649 6278 8 Size: 1629 43 Fragments arranged by size: 1659 1629 1425 889 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3285 4243 1353 Size: 1932 958 3423 Fragments arranged by size: 3423 1932 958 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4900 5274 5420 387 Size: 1064 138 3311 374 146 1280 Fragments arranged by size: 3311 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2724 3533 3546 3552 4376 4973 Size: 393 478 614 809 13 6 824 597 Cuts at: 4973 5760 6028 1239 Size: 787 268 1524 Fragments arranged by size: 1524 824 809 787 614 597 478 393 268 13 6 EcoNI CCTnn'n_nnAGG Cuts at: 2477 2477 Size: 6313 EcoRI G'AATT_C Cuts at: 3622 4000 3622 Size: 378 5935 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2165 2165 Size: 6313 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 5460 1678 Size: 3782 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 2804 2836 4954 Size: 32 135 32 881 32 319 32 2118 Cuts at: 4954 4986 5028 5060 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2118 881 319 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3657 3903 669 Size: 988 281 1719 246 3079 Fragments arranged by size: 3079 1719 988 281 246 HindIII A'AGCT_T Cuts at: 748 2182 3500 748 Size: 1434 1318 3561 Fragments arranged by size: 3561 1434 1318 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KpnI G_GTAC'C Cuts at: 3638 3638 Size: 6313 MfeI C'AATT_G Cuts at: 3912 3912 Size: 6313 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2295 2856 3066 5427 5611 1305 Size: 990 561 210 2361 184 2007 Fragments arranged by size: 2361 2007 990 561 210 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2103 10 Size: 54 1425 343 271 4220 Fragments arranged by size: 4220 1425 343 271 54 MslI CAynn'nnrTG Cuts at: 518 1166 4271 4630 4789 518 Size: 648 3105 359 159 2042 Fragments arranged by size: 3105 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3729 4406 5554 5799 1606 Size: 2123 677 1148 245 2120 Fragments arranged by size: 2123 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2028 2028 Size: 6313 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5761 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NgoMIV G'CCGG_C Cuts at: 2026 2026 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 2510 3671 5216 1132 Size: 97 1281 1161 1545 2229 Fragments arranged by size: 2229 1545 1281 1161 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6313 PciI A'CATG_T Cuts at: 5212 5212 Size: 6313 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6313 PsiI TTA'TAA Cuts at: 2393 3883 2393 Size: 1490 4823 PstI C_TGCA'G Cuts at: 830 3113 3665 830 Size: 2283 552 3478 Fragments arranged by size: 3478 2283 552 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SfcI C'TryA_G Cuts at: 826 1045 2268 2836 3109 3432 3661 4796 5477 Size: 219 1223 568 273 323 229 1135 681 Cuts at: 5477 5668 826 Size: 191 1471 Fragments arranged by size: 1471 1223 1135 681 568 323 273 229 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3640 1872 Size: 1768 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4417 5318 5580 820 Size: 197 926 2474 901 262 1553 Fragments arranged by size: 2474 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 3188 152 Size: 3036 3277 SphI G_CATG'C Cuts at: 1132 2510 3671 1132 Size: 1378 1161 3774 Fragments arranged by size: 3774 1378 1161 SspI AAT'ATT Cuts at: 5 52 2159 4237 5 Size: 47 2107 2078 2081 Fragments arranged by size: 2107 2081 2078 47 StuI AGG'CCT Cuts at: 1517 2599 1517 Size: 1082 5231 StyI C'CwwG_G Cuts at: 513 1065 2717 3752 513 Size: 552 1652 1035 3074 Fragments arranged by size: 3074 1652 1035 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4340 4357 4510 4695 5140 325 Size: 1255 2760 17 153 185 445 1498 Fragments arranged by size: 2760 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2224 3401 4559 Size: 274 80 33 51 1443 247 1177 1158 Cuts at: 4559 6271 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1177 1158 274 247 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6273 6305 6273 Size: 32 6281 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6313 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6313 XmaI C'CCGG_G Cuts at: 1870 3638 1870 Size: 1768 4545 XmnI GAAnn'nnTTC Cuts at: 2148 4442 2148 Size: 2294 4019 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI