Restriction map for
pFN21AB7811
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BcgI BclI
BfaI BfrI BglII BlnI BsaBI
BsaI BsiWI BsmI Bsp1407I BspDI
BspHI BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FspI HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 233 TCCGGA T^CCGGA
AfaI 177 GTAC GT^AC
Alw44I 1396 GTGCAC G^TGCAC
ApaLI 1396 GTGCAC G^TGCAC
Asp700I 123 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 403 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 602 GGATCC G^GATCC
BanI 947 GGCGCC G^G[CT][GA]CC
BbeI 947 GGCGCC GGCGC^C
BbrPI 133 CACGTG CAC^GTG
BbsI 382 GAAGAC GAAGAC(2/6)
BbvCI 1119 CCTCAGC CCTCAGC(-5/-2)
BciVI 1313 GTATCC GTATCC(6/5)
BglI 1341 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 382 GAAGAC GAAGAC(2/6)
BsaAI 133 CACGTG [CT]AC^GT[GA]
BseAI 233 TCCGGA T^CCGGA
BsiEI 980 CGACCG CG[GA][CT]^CG
BsmBI 281 GAGACG CGTCTC(1/5)
BspEI 233 TCCGGA T^CCGGA
BspLU11I 221 ACATGT A^CATGT
BsrBI 117 GAGCGG CCGCTC(-3/-3)
BssSI 73 CTCGTG CACGAG(-5/-1)
BstEII 1263 GGTGACC G^GT[ACGT]ACC
BstPI 1263 GGTGACC G^GT[ACGT]ACC
EcoO65I 1263 GGTGACC G^GT[ACGT]ACC
FseI 1075 GGCCGGCC GGCCGG^CC
HaeII 947 GGCGCC [GA]GCGC^[CT]
HincII 556 GTCAAC GT[CT]^[GA]AC
HindII 556 GTCAAC GT[CT]^[GA]AC
KasI 947 GGCGCC G^GCGCC
MroI 233 TCCGGA T^CCGGA
NarI 947 GGCGCC GG^CGCC
NcoI 827 CCATGG C^CATGG
PflFI 403 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 59 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 133 CACGTG CAC^GTG
PmlI 133 CACGTG CAC^GTG
PstI 1192 CTGCAG CTGCA^G
RsaI 177 GTAC GT^AC
ScaI 176 AGTACT AGT^ACT
SfoI 947 GGCGCC GGC^GCC
SmlI 334 CTTGAG C^T[CT][GA]AG
SphI 534 GCATGC GCATG^C
StuI 1348 AGGCCT AGG^CCT
TfiI 1378 GATTC G^A[AT]TC
Tth111I 403 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 59 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 123 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 24 CGCG CG^CG
96 CGCG
AcyI 947 GGCGCC G[GA]^CG[CT]C
1048 GACGCC
AflIII 134 ACGTGT A^C[GA][CT]GT
221 ACATGT
AlwNI 571 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1411 CAGCCGCTG
BlpI 650 GCTCAGC GC^T[ACGT]AGC
1203 GCTGAGC
Bpu1102I 650 GCTCAGC GC^T[ACGT]AGC
1203 GCTGAGC
BsaHI 947 GGCGCC G[GA]^CG[CT]C
1048 GACGCC
BsaWI 233 TCCGGA [AT]^CCGG[AT]
599 ACCGGA
BseRI 895 GAGGAG GAGGAG(10/8)
931 GAGGAG
BsgI 430 GTGCAG GTGCAG(16/14)
589 GTGCAG
BstAPI 1194 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1389 GCAGCTGGTGC
BstF5I 999 CATCC GGATG(2/0)
1164 GGATG
BstUI 24 CGCG CG^CG
96 CGCG
BstXI 467 CCAAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
821 CCAGCCCCATGG
CelII 650 GCTCAGC GC^T[ACGT]AGC
1203 GCTGAGC
DrdI 357 GACCAGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1573 GACCCCACTGTC
EarI 375 GAAGAG CTCTTC(1/4)
615 GAAGAG
FokI 999 CATCC GGATG(9/13)
1164 GGATG
Hin1I 947 GGCGCC G[GA]^CG[CT]C
1048 GACGCC
Ksp632I 375 GAAGAG CTCTTC(1/4)
615 GAAGAG
MslI 487 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1159 CACTTGGATG
MvnI 24 CGCG CG^CG
96 CGCG
NaeI 677 GCCGGC GCC^GGC
1076 GCCGGC
NgoMIV 677 GCCGGC G^CCGGC
1076 GCCGGC
NspI 221 ACATGT [GA]CATG^[CT]
534 GCATGC
PpuMI 1523 AGGACCC [GA]G^G[AT]CC[CT]
1571 AGGACCC
PvuII 433 CAGCTG CAG^CTG
1390 CAGCTG
SacI 1147 GAGCTC GAGCT^C
1321 GAGCTC
SfcI 738 CTACAG C^T[GA][CT]AG
1192 CTGCAG
SmaI 923 CCCGGG CCC^GGG
943 CCCGGG
TaqI 140 TCGA T^CGA
251 TCGA
TthHB8I 140 TCGA T^CGA
251 TCGA
XcmI 869 CCAGCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1232 CCAGTGCCCTGCTGG
XmaCI 923 CCCGGG C^CCGGG
943 CCCGGG
XmaI 923 CCCGGG C^CCGGG
943 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AluI AlwI ApaI AspHI
AvaI AvaII BalI BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
XhoII