(Circular) (Six-base) MAPSORT of: pFN21AB7811.seq Check: 5301 from: 1 to: 6292 pFN21AB7811 6292 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3635 1937 Size: 1698 4594 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 3208 4355 5739 6007 1632 Size: 767 809 1147 1384 268 1917 Fragments arranged by size: 1917 1384 1147 809 767 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 2139 2226 5191 6168 1203 Size: 936 87 2965 977 1327 Fragments arranged by size: 2965 1327 977 936 87 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2556 3482 1184 Size: 1372 926 3994 Fragments arranged by size: 3994 1372 926 AlwNI CAG_nnn'CTG Cuts at: 2581 3421 4035 5607 2581 Size: 840 614 1572 3266 Fragments arranged by size: 3266 1572 840 614 ApaI G_GGCC'C Cuts at: 2024 2040 2720 2024 Size: 16 680 5596 Fragments arranged by size: 5596 680 16 ApaLI G'TGCA_C Cuts at: 3401 4349 5505 3401 Size: 948 1156 4188 Fragments arranged by size: 4188 1156 948 ApoI r'AATT_y Cuts at: 1493 3601 3827 3979 6088 1493 Size: 2108 226 152 2109 1697 Fragments arranged by size: 2109 2108 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2713 2928 2948 3359 3617 1870 Size: 73 176 594 215 20 411 258 4545 Fragments arranged by size: 4545 594 411 258 215 176 73 20 BamHI G'GATC_C Cuts at: 2607 3622 3989 2607 Size: 1015 367 4910 Fragments arranged by size: 4910 1015 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2952 3613 5068 618 Size: 325 217 627 1165 661 1455 1842 Fragments arranged by size: 1842 1455 1165 661 627 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2040 2120 2720 2907 2927 3156 3330 Size: 633 16 80 600 187 20 229 174 Cuts at: 3330 3348 3497 3611 1391 Size: 18 149 114 4072 Fragments arranged by size: 4072 633 600 229 187 174 149 114 80 20 18 16 BbeI G_GCGC'C Cuts at: 2956 2956 Size: 6292 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2394 928 Size: 971 495 4826 Fragments arranged by size: 4826 971 495 BbvCI CC'TCA_GC Cuts at: 3125 3125 Size: 6292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4483 4517 1903 Size: 34 2546 34 3678 Fragments arranged by size: 3678 2546 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3329 4184 5394 3329 Size: 855 1210 4227 Fragments arranged by size: 4227 1210 855 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3352 4903 136 Size: 107 122 71 631 605 1680 1551 1525 Fragments arranged by size: 1680 1551 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 2656 3209 3709 2656 Size: 553 500 5239 Fragments arranged by size: 5239 553 500 Bme1580I G_kGCm'C Cuts at: 1790 2024 2040 2720 3244 3279 3405 4353 5509 Size: 234 16 680 524 35 126 948 1156 Cuts at: 5509 1790 Size: 2573 Fragments arranged by size: 2573 1156 948 680 524 234 126 35 16 BmgBI CAC'GTC Cuts at: 1318 2434 2704 1318 Size: 1116 270 4906 Fragments arranged by size: 4906 1116 270 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4023 4981 442 Size: 416 53 83 399 24 2606 958 1753 Fragments arranged by size: 2606 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2134 2717 2737 4952 1239 Size: 895 583 20 2215 2579 Fragments arranged by size: 2579 2215 895 583 20 Bpu10I CC'TnA_GC Cuts at: 3125 3125 Size: 6292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2360 4417 5282 5580 2360 Size: 2057 865 298 3072 Fragments arranged by size: 3072 2057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4955 882 Size: 617 3456 2219 Fragments arranged by size: 3456 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2140 4083 493 Size: 711 936 1943 2702 Fragments arranged by size: 2702 1943 936 711 BsaBI GATnn'nnATC Cuts at: 6286 6286 Size: 6292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2953 3054 4099 4481 Size: 53 83 186 936 1420 101 1045 382 Cuts at: 4481 5116 275 Size: 635 1451 Fragments arranged by size: 1451 1420 1045 936 635 382 186 101 83 53 BsaWI w'CCGG_w Cuts at: 2238 2604 4725 5123 5397 5544 5955 2238 Size: 366 2121 398 274 147 411 2575 Fragments arranged by size: 2575 2121 411 398 366 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2735 2765 3425 3455 1178 Size: 30 165 30 1332 30 660 30 4015 Fragments arranged by size: 4015 1332 660 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2915 2951 1186 Size: 1729 36 4527 Fragments arranged by size: 4527 1729 36 BseYI C'CCAG_C Cuts at: 2039 2544 2737 2909 3484 5495 5991 2039 Size: 505 193 172 575 2011 496 2340 Fragments arranged by size: 2340 2011 575 505 496 193 172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 2615 1163 Size: 573 720 159 4840 Fragments arranged by size: 4840 720 573 159 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2988 3742 4503 5531 1540 Size: 275 186 987 754 761 1028 2301 Fragments arranged by size: 2301 1028 987 761 754 275 186 BsiHKAI G_wGCw'C Cuts at: 2703 3156 3330 3405 4353 4438 5509 2703 Size: 453 174 75 948 85 1071 3486 Fragments arranged by size: 3486 1071 948 453 174 85 75 BsmI GAATG_Cn' Cuts at: 3803 3896 3803 Size: 93 6199 BsmBI CGTCTCn'nnnn_ Cuts at: 2280 2280 Size: 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2040 2120 2703 2720 2907 2927 Size: 399 234 16 80 583 17 187 20 Cuts at: 2927 3156 3244 3279 3330 3348 3405 3497 3611 Size: 229 88 35 51 18 57 92 114 Cuts at: 3611 4353 4438 5509 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 583 399 234 229 187 114 92 88 85 80 57 51 35 20 18 17 16 BspEI T'CCGG_A Cuts at: 2238 5955 2238 Size: 3717 2575 BspHI T'CATG_A Cuts at: 4181 5911 4181 Size: 1730 4562 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3031 3204 3474 3647 844 Size: 2187 173 270 173 3489 Fragments arranged by size: 3489 2187 270 173 173 BsrBI CCG'CTC Cuts at: 1394 1823 2124 4179 1394 Size: 429 301 2055 3507 Fragments arranged by size: 3507 2055 429 301 BsrDI GCAATG_nn' Cuts at: 65 1248 4787 4961 65 Size: 1183 3539 174 1396 Fragments arranged by size: 3539 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2682 3081 3469 4936 5123 1700 Size: 110 872 399 388 1467 187 2869 Fragments arranged by size: 2869 1467 872 399 388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 2078 4352 5364 2078 Size: 2274 1012 3006 Fragments arranged by size: 3006 2274 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3205 3400 794 Size: 2411 195 3686 Fragments arranged by size: 3686 2411 195 BstBI TT'CG_AA Cuts at: 5096 5096 Size: 6292 BstEII G'GTnAC_C Cuts at: 1184 3268 1184 Size: 2084 4208 BstXI CCAn_nnnn'nTGG Cuts at: 2479 2833 2479 Size: 354 5938 BstYI r'GATC_y Cuts at: 1972 2008 2397 2607 2628 3622 3989 4374 4391 Size: 36 389 210 21 994 367 385 17 Cuts at: 4391 5843 5929 5941 6287 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 994 389 385 367 346 210 86 36 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2707 2832 2964 3217 3444 Size: 552 939 12 691 125 132 253 227 Cuts at: 3444 513 Size: 3361 Fragments arranged by size: 3361 939 691 552 253 227 132 125 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5553 Fragments arranged by size: 5553 683 56 BtsI GCAGTG_nn' Cuts at: 3808 4602 4622 3808 Size: 794 20 5478 Fragments arranged by size: 5478 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6257 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2368 3584 5299 809 Size: 507 1052 1216 1715 1802 Fragments arranged by size: 1802 1715 1216 1052 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 2323 2481 Size: 54 1425 50 275 18 372 121 158 Cuts at: 2481 2520 2778 3312 3739 4628 6257 8 Size: 39 258 534 427 889 1629 43 Fragments arranged by size: 1629 1425 889 534 427 372 275 258 158 121 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2375 2615 4222 1353 Size: 1022 240 1607 3423 Fragments arranged by size: 3423 1607 1022 240 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2390 4879 5253 5399 387 Size: 1064 138 801 2489 374 146 1280 Fragments arranged by size: 2489 1280 1064 801 374 146 138 EcoICRI GAG'CTC Cuts at: 3154 3328 3154 Size: 174 6118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2134 2399 2717 2737 3208 4355 4952 Size: 393 502 265 318 20 471 1147 597 Cuts at: 4952 5739 6007 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1147 787 597 502 471 393 318 268 265 20 EcoO109I rG'GnC_Cy Cuts at: 2021 2036 2717 2865 3529 3577 2021 Size: 15 681 148 664 48 4736 Fragments arranged by size: 4736 681 664 148 48 15 EcoRI G'AATT_C Cuts at: 3601 3979 3601 Size: 378 5914 FseI GG_CCGG'CC Cuts at: 3085 3085 Size: 6292 FspI TGC'GCA Cuts at: 4798 4798 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 2956 5439 1678 Size: 1278 2483 2531 Fragments arranged by size: 2531 2483 1278 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2639 2671 2781 2813 3317 Size: 32 135 32 1067 32 110 32 504 Cuts at: 3317 3349 3425 3457 4933 4965 5007 5039 1373 Size: 32 76 32 1476 32 42 32 2626 Fragments arranged by size: 2626 1476 1067 504 135 110 76 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2563 3636 3882 669 Size: 988 281 625 1073 246 3079 Fragments arranged by size: 3079 1073 988 625 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6292 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KasI G'GCGC_C Cuts at: 2952 2952 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2826 3207 5406 5590 1305 Size: 1521 381 2199 184 2007 Fragments arranged by size: 2199 2007 1521 381 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2204 2325 2483 2522 2780 Size: 54 1425 343 372 121 158 39 258 Cuts at: 2780 3314 10 Size: 534 2988 Fragments arranged by size: 2988 1425 534 372 343 258 158 121 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2496 3168 4250 4609 4768 518 Size: 648 1330 672 1082 359 159 2042 Fragments arranged by size: 2042 1330 1082 672 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2440 3397 3421 3708 4385 5533 5778 1606 Size: 834 957 24 287 677 1148 245 2120 Fragments arranged by size: 2120 1148 957 834 677 287 245 24 NaeI GCC'GGC Cuts at: 2684 3083 2684 Size: 399 5893 NarI GG'CG_CC Cuts at: 2953 2953 Size: 6292 NcoI C'CATG_G Cuts at: 513 1065 2004 2832 513 Size: 552 939 828 3973 Fragments arranged by size: 3973 939 828 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NgoMIV G'CCGG_C Cuts at: 2682 3081 2682 Size: 399 5893 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 2230 2543 3650 5195 1132 Size: 97 1001 313 1107 1545 2229 Fragments arranged by size: 2229 1545 1107 1001 313 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6292 PciI A'CATG_T Cuts at: 2226 5191 2226 Size: 2965 3327 PflMI CCAn_nnn'nTGG Cuts at: 1108 2070 1108 Size: 962 5330 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PmlI CAC'GTG Cuts at: 2140 2140 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3529 3577 3529 Size: 48 6244 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PspOMI G'GGCC_C Cuts at: 2020 2036 2716 2020 Size: 16 680 5596 Fragments arranged by size: 5596 680 16 PstI C_TGCA'G Cuts at: 830 3201 3644 830 Size: 2371 443 3478 Fragments arranged by size: 3478 2371 443 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 PvuII CAG'CTG Cuts at: 2440 3397 2440 Size: 957 5335 SacI G_AGCT'C Cuts at: 3156 3330 3156 Size: 174 6118 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 2183 4540 6252 2183 Size: 2357 1712 2223 Fragments arranged by size: 2357 2223 1712 SfcI C'TryA_G Cuts at: 826 1045 2743 3197 3640 4775 5456 5647 826 Size: 219 1698 454 443 1135 681 191 1471 Fragments arranged by size: 1698 1471 1135 681 454 443 219 191 SfoI GGC'GCC Cuts at: 2954 2954 Size: 6292 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 2930 2950 3619 1872 Size: 1058 20 669 4545 Fragments arranged by size: 4545 1058 669 20 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 4396 5297 5559 820 Size: 197 926 396 2057 901 262 1553 Fragments arranged by size: 2057 1553 926 901 396 262 197 SnaBI TAC'GTA Cuts at: 493 4083 493 Size: 3590 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 2543 3650 1132 Size: 1411 1107 3774 Fragments arranged by size: 3774 1411 1107 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 3355 1517 Size: 1838 4454 StyI C'CwwG_G Cuts at: 513 1065 2004 2163 2832 3573 3731 513 Size: 552 939 159 669 741 158 3074 Fragments arranged by size: 3074 939 741 669 552 159 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2322 4319 4336 4489 4674 5119 325 Size: 1255 742 1997 17 153 185 445 1498 Fragments arranged by size: 1997 1498 1255 742 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 4538 6250 Size: 274 80 33 51 1443 204 2357 1712 Cuts at: 6250 96 Size: 138 Fragments arranged by size: 2357 1712 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6252 6284 6252 Size: 32 6260 Tth111I GACn'n_nGTC Cuts at: 1316 2411 1316 Size: 1095 5197 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2881 3244 2881 Size: 363 5929 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 2928 2948 3617 1870 Size: 1058 20 669 4545 Fragments arranged by size: 4545 1058 669 20 XmnI GAAnn'nnTTC Cuts at: 2132 4421 2132 Size: 2289 4003 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI