Restriction map for
pFN21AB7811
(Linear) (Six-base) MAPSORT of: KIBB7811.seq Check: 6497 from: 1 to: 1587
KIBB7811.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1203 1587
Size: 394 809 384
Fragments arranged by size:
809 394 384
AflIII A'CryG_T
Cuts at: 0 134 221 1587
Size: 134 87 1366
Fragments arranged by size:
1366 134 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 551 1477 1587
Size: 551 926 110
Fragments arranged by size:
926 551 110
AlwNI CAG_nnn'CTG
Cuts at: 0 576 1416 1587
Size: 576 840 171
Fragments arranged by size:
840 576 171
ApaI G_GGCC'C
Cuts at: 0 19 35 715 1587
Size: 19 16 680 872
Fragments arranged by size:
872 680 19 16
ApaLI G'TGCA_C
Cuts at: 0 1396 1587
Size: 1396 191
AvaI C'yCGr_G
Cuts at: 0 114 708 923 943 1354 1587
Size: 114 594 215 20 411 233
Fragments arranged by size:
594 411 233 215 114 20
BamHI G'GATC_C
Cuts at: 0 602 1587
Size: 602 985
BanI G'GyrC_C
Cuts at: 0 947 1587
Size: 947 640
BanII G_rGCy'C
Cuts at: 0 19 35 115 715 902 922 1151 1325
Size: 19 16 80 600 187 20 229 174
Cuts at: 1325 1343 1492 1587
Size: 18 149 95
Fragments arranged by size:
600 229 187 174 149 95 80 20
19 18 16
BbeI G_GCGC'C
Cuts at: 0 951 1587
Size: 951 636
BbsI GAAGACnn'nnnn_
Cuts at: 0 389 1587
Size: 389 1198
BbvCI CC'TCA_GC
Cuts at: 0 1120 1587
Size: 1120 467
BciVI GTATCCnnnnn_n'
Cuts at: 0 1324 1587
Size: 1324 263
BglI GCCn_nnn'nGGC
Cuts at: 0 1347 1587
Size: 1347 240
BlpI GC'TnA_GC
Cuts at: 0 651 1204 1587
Size: 651 553 383
Fragments arranged by size:
651 553 383
Bme1580I G_kGCm'C
Cuts at: 0 19 35 715 1239 1274 1400 1587
Size: 19 16 680 524 35 126 187
Fragments arranged by size:
680 524 187 126 35 19 16
BmgBI CAC'GTC
Cuts at: 0 429 699 1587
Size: 429 270 888
Fragments arranged by size:
888 429 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 712 732 1587
Size: 129 583 20 855
Fragments arranged by size:
855 583 129 20
Bpu10I CC'TnA_GC
Cuts at: 0 1120 1587
Size: 1120 467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1587
Size: 355 1232
BsaAI yAC'GTr
Cuts at: 0 135 1587
Size: 135 1452
BsaHI Gr'CG_yC
Cuts at: 0 948 1049 1587
Size: 948 101 538
Fragments arranged by size:
948 538 101
BsaWI w'CCGG_w
Cuts at: 0 233 599 1587
Size: 233 366 988
Fragments arranged by size:
988 366 233
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 1420 1450 1587
Size: 730 30 660 30 137
Fragments arranged by size:
730 660 137 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 910 946 1587
Size: 910 36 641
Fragments arranged by size:
910 641 36
BseYI C'CCAG_C
Cuts at: 0 34 539 732 904 1479 1587
Size: 34 505 193 172 575 108
Fragments arranged by size:
575 505 193 172 108 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 610 1587
Size: 451 159 977
Fragments arranged by size:
977 451 159
BsiEI CG_ry'CG
Cuts at: 0 983 1587
Size: 983 604
BsiHKAI G_wGCw'C
Cuts at: 0 698 1151 1325 1400 1587
Size: 698 453 174 75 187
Fragments arranged by size:
698 453 187 174 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 275 1587
Size: 275 1312
Bsp1286I G_dGCh'C
Cuts at: 0 19 35 115 698 715 902 922 1151
Size: 19 16 80 583 17 187 20 229
Cuts at: 1151 1239 1274 1325 1343 1400 1492 1587
Size: 88 35 51 18 57 92 95
Fragments arranged by size:
583 229 187 95 92 88 80 57
51 35 20 19 18 17 16
BspEI T'CCGG_A
Cuts at: 0 233 1587
Size: 233 1354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1026 1199 1469 1587
Size: 1026 173 270 118
Fragments arranged by size:
1026 270 173 118
BsrBI CCG'CTC
Cuts at: 0 119 1587
Size: 119 1468
BsrFI r'CCGG_y
Cuts at: 0 677 1076 1464 1587
Size: 677 399 388 123
Fragments arranged by size:
677 399 388 123
BssSI C'ACGA_G
Cuts at: 0 73 1587
Size: 73 1514
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1200 1395 1587
Size: 1200 195 192
Fragments arranged by size:
1200 195 192
BstEII G'GTnAC_C
Cuts at: 0 1263 1587
Size: 1263 324
BstXI CCAn_nnnn'nTGG
Cuts at: 0 474 828 1587
Size: 474 354 759
Fragments arranged by size:
759 474 354
BstYI r'GATC_y
Cuts at: 0 3 392 602 623 1587
Size: 3 389 210 21 964
Fragments arranged by size:
964 389 210 21 3
BtgI C'CryG_G
Cuts at: 0 11 702 827 959 1212 1439 1587
Size: 11 691 125 132 253 227 148
Fragments arranged by size:
691 253 227 148 132 125 11
DrdI GACnn_nn'nnGTC
Cuts at: 0 363 1579 1587
Size: 363 1216 8
Fragments arranged by size:
1216 363 8
EaeI y'GGCC_r
Cuts at: 0 197 318 476 515 773 1307 1587
Size: 197 121 158 39 258 534 280
Fragments arranged by size:
534 280 258 197 158 121 39
EarI CTCTTCn'nnn_
Cuts at: 0 370 610 1587
Size: 370 240 977
Fragments arranged by size:
977 370 240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 385 1587
Size: 385 1202
EcoICRI GAG'CTC
Cuts at: 0 1149 1323 1587
Size: 1149 174 264
Fragments arranged by size:
1149 264 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 394 712 732 1203 1587
Size: 129 265 318 20 471 384
Fragments arranged by size:
471 384 318 265 129 20
EcoO109I rG'GnC_Cy
Cuts at: 0 16 31 712 860 1524 1572 1587
Size: 16 15 681 148 664 48 15
Fragments arranged by size:
681 664 148 48 16 15 15
FseI GG_CCGG'CC
Cuts at: 0 1080 1587
Size: 1080 507
HaeII r_GCGC'y
Cuts at: 0 951 1587
Size: 951 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 634 666 776 808 1312 1344 1420 1452
Size: 634 32 110 32 504 32 76 32
Cuts at: 1452 1587
Size: 135
Fragments arranged by size:
634 504 135 110 76 32 32 32
32
HincII GTy'rAC
Cuts at: 0 558 1587
Size: 558 1029
KasI G'GCGC_C
Cuts at: 0 947 1587
Size: 947 640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1202 1587
Size: 821 381 385
Fragments arranged by size:
821 385 381
MscI TGG'CCA
Cuts at: 0 199 320 478 517 775 1309 1587
Size: 199 121 158 39 258 534 278
Fragments arranged by size:
534 278 258 199 158 121 39
MslI CAynn'nnrTG
Cuts at: 0 491 1163 1587
Size: 491 672 424
Fragments arranged by size:
672 491 424
MspA1I CmG'CkG
Cuts at: 0 435 1392 1416 1587
Size: 435 957 24 171
Fragments arranged by size:
957 435 171 24
NaeI GCC'GGC
Cuts at: 0 679 1078 1587
Size: 679 399 509
Fragments arranged by size:
679 509 399
NarI GG'CG_CC
Cuts at: 0 948 1587
Size: 948 639
NcoI C'CATG_G
Cuts at: 0 827 1587
Size: 827 760
NgoMIV G'CCGG_C
Cuts at: 0 677 1076 1587
Size: 677 399 511
Fragments arranged by size:
677 511 399
NspI r_CATG'y
Cuts at: 0 225 538 1587
Size: 225 313 1049
Fragments arranged by size:
1049 313 225
PciI A'CATG_T
Cuts at: 0 221 1587
Size: 221 1366
PflMI CCAn_nnn'nTGG
Cuts at: 0 65 1587
Size: 65 1522
PmlI CAC'GTG
Cuts at: 0 135 1587
Size: 135 1452
PpuMI rG'GwC_Cy
Cuts at: 0 1524 1572 1587
Size: 1524 48 15
Fragments arranged by size:
1524 48 15
PspOMI G'GGCC_C
Cuts at: 0 15 31 711 1587
Size: 15 16 680 876
Fragments arranged by size:
876 680 16 15
PstI C_TGCA'G
Cuts at: 0 1196 1587
Size: 1196 391
PvuII CAG'CTG
Cuts at: 0 435 1392 1587
Size: 435 957 195
Fragments arranged by size:
957 435 195
SacI G_AGCT'C
Cuts at: 0 1151 1325 1587
Size: 1151 174 262
Fragments arranged by size:
1151 262 174
ScaI AGT'ACT
Cuts at: 0 178 1587
Size: 178 1409
SfcI C'TryA_G
Cuts at: 0 738 1192 1587
Size: 738 454 395
Fragments arranged by size:
738 454 395
SfoI GGC'GCC
Cuts at: 0 949 1587
Size: 949 638
SmaI CCC'GGG
Cuts at: 0 925 945 1587
Size: 925 20 642
Fragments arranged by size:
925 642 20
SmlI C'TyrA_G
Cuts at: 0 334 1587
Size: 334 1253
SphI G_CATG'C
Cuts at: 0 538 1587
Size: 538 1049
StuI AGG'CCT
Cuts at: 0 1350 1587
Size: 1350 237
StyI C'CwwG_G
Cuts at: 0 158 827 1568 1587
Size: 158 669 741 19
Fragments arranged by size:
741 669 158 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 317 1587
Size: 317 1270
TatI w'GTAC_w
Cuts at: 0 176 1587
Size: 176 1411
Tth111I GACn'n_nGTC
Cuts at: 0 406 1587
Size: 406 1181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 876 1239 1587
Size: 876 363 348
Fragments arranged by size:
876 363 348
XmaI C'CCGG_G
Cuts at: 0 923 943 1587
Size: 923 20 644
Fragments arranged by size:
923 644 20
XmnI GAAnn'nnTTC
Cuts at: 0 127 1587
Size: 127 1460
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI
BstYI BtgI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I FseI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmlI PpuMI PspOMI PstI PvuII SacI ScaI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BclI BfrBI BglII BmrI BmtI BplI
BsaI BsaBI BsiWI BsmI BspHI BsrDI BsrGI BssHII
BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EcoNI EcoRI EcoRV FalI FspI FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TstI XbaI XhoI ZraI