(Circular) (Six-base) MAPSORT of: pFN21AE0156.seq Check: 4377 from: 1 to: 5488 pFN21AE0156 5488 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3298 278 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 1937 Size: 894 4594 Acc65I G'GTAC_C Cuts at: 2809 2809 Size: 5488 AclI AA'CG_TT Cuts at: 1563 3615 3988 1563 Size: 2052 373 3063 Fragments arranged by size: 3063 2052 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2751 3551 4935 5203 1632 Size: 1119 800 1384 268 1917 Fragments arranged by size: 1917 1384 1119 800 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5291 AflIII A'CryG_T Cuts at: 1203 2229 2346 4387 5364 1203 Size: 1026 117 2041 977 1327 Fragments arranged by size: 2041 1327 1026 977 117 AgeI A'CCGG_T Cuts at: 4319 4319 Size: 5488 AhdI GACnn_n'nnGTC Cuts at: 4217 4217 Size: 5488 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5488 AlwNI CAG_nnn'CTG Cuts at: 3231 4803 3231 Size: 1572 3916 ApaLI G'TGCA_C Cuts at: 3545 4701 3545 Size: 1156 4332 ApoI r'AATT_y Cuts at: 1493 2797 3023 3175 5284 1493 Size: 1304 226 152 2109 1697 Fragments arranged by size: 2109 1697 1304 226 152 AseI AT'TA_AT Cuts at: 160 1032 4042 160 Size: 872 3010 1606 Fragments arranged by size: 3010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 AvaI C'yCGr_G Cuts at: 1870 1943 2199 2556 2813 1870 Size: 73 256 357 257 4545 Fragments arranged by size: 4545 357 257 256 73 BamHI G'GATC_C Cuts at: 2577 2818 3185 2577 Size: 241 367 4880 Fragments arranged by size: 4880 367 241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2253 2393 2809 4264 618 Size: 325 217 627 466 140 416 1455 1842 Fragments arranged by size: 1842 1455 627 466 416 325 217 140 BanII G_rGCy'C Cuts at: 1391 2807 1391 Size: 1416 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4517 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3679 3713 1903 Size: 34 1742 34 3678 Fragments arranged by size: 3678 1742 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2735 3380 4590 2735 Size: 645 1210 3633 Fragments arranged by size: 3633 1210 645 BclI T'GATC_A Cuts at: 1548 1548 Size: 5488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2222 4099 136 Size: 107 122 71 631 605 550 1877 1525 Fragments arranged by size: 1877 1525 631 605 550 122 107 71 BglII A'GATC_T Cuts at: 5483 5483 Size: 5488 BlpI GC'TnA_GC Cuts at: 2905 2905 Size: 5488 Bme1580I G_kGCm'C Cuts at: 1790 2396 3549 4705 1790 Size: 606 1153 1156 2573 Fragments arranged by size: 2573 1156 1153 606 BmgBI CAC'GTC Cuts at: 1318 2230 1318 Size: 912 4576 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2371 3219 4177 Size: 416 53 83 399 24 954 848 958 Cuts at: 4177 442 Size: 1753 Fragments arranged by size: 1753 958 954 848 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2003 2547 2793 4148 1239 Size: 764 544 246 1355 2579 Fragments arranged by size: 2579 1355 764 544 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2372 3613 4478 4776 2372 Size: 1241 865 298 3084 Fragments arranged by size: 3084 1241 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4151 882 Size: 617 2652 2219 Fragments arranged by size: 2652 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3279 493 Size: 711 2075 2702 Fragments arranged by size: 2702 2075 711 BsaBI GATnn'nnATC Cuts at: 5482 5482 Size: 5488 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3295 3677 4312 Size: 53 83 186 936 476 1286 382 635 Cuts at: 4312 275 Size: 1451 Fragments arranged by size: 1451 1286 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3921 4319 4593 4740 5151 3921 Size: 398 274 147 411 4258 Fragments arranged by size: 4258 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5263 Fragments arranged by size: 5263 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5488 BseYI C'CCAG_C Cuts at: 2167 4691 5187 2167 Size: 2524 496 2468 Fragments arranged by size: 2524 2468 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2291 2327 1163 Size: 573 555 36 4324 Fragments arranged by size: 4324 573 555 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2077 2938 3699 4727 1540 Size: 275 186 76 861 761 1028 2301 Fragments arranged by size: 2301 1028 861 761 275 186 76 BsiHKAI G_wGCw'C Cuts at: 3549 3634 4705 3549 Size: 85 1071 4332 Fragments arranged by size: 4332 1071 85 BsmI GAATG_Cn' Cuts at: 2717 2999 3092 2717 Size: 282 93 5113 Fragments arranged by size: 5113 282 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2016 2147 2016 Size: 131 5357 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 2807 3549 3634 4705 1391 Size: 399 606 411 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 606 411 399 85 BspEI T'CCGG_A Cuts at: 5151 5151 Size: 5488 BspHI T'CATG_A Cuts at: 3377 5107 3377 Size: 1730 3758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2843 844 Size: 1999 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2034 2067 2081 3375 1394 Size: 429 211 33 14 1294 3507 Fragments arranged by size: 3507 1294 429 211 33 14 BsrDI GCAATG_nn' Cuts at: 65 1248 2607 3983 4157 65 Size: 1183 1359 1376 174 1396 Fragments arranged by size: 1396 1376 1359 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4132 4319 1700 Size: 110 2322 187 2869 Fragments arranged by size: 2869 2322 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5488 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5488 BssSI C'ACGA_G Cuts at: 3548 4560 3548 Size: 1012 4476 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5488 BstBI TT'CG_AA Cuts at: 4292 4292 Size: 5488 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5488 BstYI r'GATC_y Cuts at: 1972 2577 2818 3185 3570 3587 5039 5125 5137 Size: 605 241 367 385 17 1452 86 12 Cuts at: 5137 5483 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 605 385 367 346 241 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2694 2694 Size: 5488 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3997 Fragments arranged by size: 3997 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4749 Fragments arranged by size: 4749 683 56 BtsI GCAGTG_nn' Cuts at: 3004 3798 3818 3004 Size: 794 20 4674 Fragments arranged by size: 4674 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5453 DraI TTT'AAA Cuts at: 2792 2792 Size: 5488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4495 809 Size: 507 3179 1802 Fragments arranged by size: 3179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 2097 2935 Size: 54 1425 50 275 18 244 23 838 Cuts at: 2935 3824 5453 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 838 275 244 54 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 2074 2935 1537 Size: 275 262 861 4090 Fragments arranged by size: 4090 861 275 262 EarI CTCTTCn'nnn_ Cuts at: 1353 3418 1353 Size: 2065 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4075 4449 4595 387 Size: 1064 138 2486 374 146 1280 Fragments arranged by size: 2486 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2003 2547 2751 2793 3551 4148 4935 Size: 393 371 544 204 42 758 597 787 Cuts at: 4935 5203 1239 Size: 268 1524 Fragments arranged by size: 1524 787 758 597 544 393 371 268 204 42 EcoO109I rG'GnC_Cy Cuts at: 2689 2754 2689 Size: 65 5423 EcoRI G'AATT_C Cuts at: 2797 3175 2797 Size: 378 5110 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2413 2413 Size: 5488 FspI TGC'GCA Cuts at: 3994 3994 Size: 5488 HaeII r_GCGC'y Cuts at: 1678 4635 1678 Size: 2957 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2562 2594 4129 4161 4203 Size: 32 135 32 990 32 1535 32 42 Cuts at: 4203 4235 1373 Size: 32 2626 Fragments arranged by size: 2626 1535 990 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2383 2832 3078 669 Size: 988 281 445 449 246 3079 Fragments arranged by size: 3079 988 449 445 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5488 HpaI GTT'AAC Cuts at: 3078 3078 Size: 5488 KpnI G_GTAC'C Cuts at: 2813 2813 Size: 5488 MfeI C'AATT_G Cuts at: 3087 3087 Size: 5488 MluI A'CGCG_T Cuts at: 5364 5364 Size: 5488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4602 4786 1305 Size: 3297 184 2007 Fragments arranged by size: 3297 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3666 Fragments arranged by size: 3666 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3446 3805 3964 518 Size: 648 2280 359 159 2042 Fragments arranged by size: 2280 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2144 2583 2904 3581 4729 4974 1606 Size: 538 439 321 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 538 439 321 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3997 Fragments arranged by size: 3997 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5488 NotI GC'GGCC_GC Cuts at: 2935 2935 Size: 5488 NspI r_CATG'y Cuts at: 1132 1229 2350 2846 4391 1132 Size: 97 1121 496 1545 2229 Fragments arranged by size: 2229 1545 1121 496 97 PasI CC'CwG_GG Cuts at: 1348 2600 1348 Size: 1252 4236 PciI A'CATG_T Cuts at: 2346 4387 2346 Size: 2041 3447 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5488 PmeI GTTT'AAAC Cuts at: 2792 2792 Size: 5488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5070 5102 3557 Size: 32 1481 32 3943 Fragments arranged by size: 3943 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2689 2754 2689 Size: 65 5423 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5488 PsiI TTA'TAA Cuts at: 3058 3058 Size: 5488 PstI C_TGCA'G Cuts at: 830 2419 2840 830 Size: 1589 421 3478 Fragments arranged by size: 3478 1589 421 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5488 SalI G'TCGA_C Cuts at: 1936 2830 1936 Size: 894 4594 SanDI GG'GwC_CC Cuts at: 2689 2689 Size: 5488 SbfI CC_TGCA'GG Cuts at: 2840 2840 Size: 5488 ScaI AGT'ACT Cuts at: 3736 5448 3736 Size: 1712 3776 SfcI C'TryA_G Cuts at: 826 1045 2415 2836 3971 4652 4843 826 Size: 219 1370 421 1135 681 191 1471 Fragments arranged by size: 1471 1370 1135 681 421 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5488 SmaI CCC'GGG Cuts at: 1872 2558 2815 1872 Size: 686 257 4545 Fragments arranged by size: 4545 686 257 SmlI C'TyrA_G Cuts at: 820 1017 1943 2387 3592 4493 4755 820 Size: 197 926 444 1205 901 262 1553 Fragments arranged by size: 1553 1205 926 901 444 262 197 SnaBI TAC'GTA Cuts at: 493 3279 493 Size: 2786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5488 SphI G_CATG'C Cuts at: 1132 2846 1132 Size: 1714 3774 SspI AAT'ATT Cuts at: 5 52 3412 5 Size: 47 3360 2081 Fragments arranged by size: 3360 2081 47 StuI AGG'CCT Cuts at: 1517 2276 1517 Size: 759 4729 StyI C'CwwG_G Cuts at: 513 1065 2004 2046 2520 2927 513 Size: 552 939 42 474 407 3074 Fragments arranged by size: 3074 939 552 474 407 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2339 2632 3515 3532 3685 3870 4315 Size: 1255 759 293 883 17 153 185 445 Cuts at: 4315 325 Size: 1498 Fragments arranged by size: 1498 1255 883 759 445 293 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3734 5446 96 Size: 274 80 33 51 1443 1757 1712 138 Fragments arranged by size: 1757 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5448 5480 5448 Size: 32 5456 Tth111I GACn'n_nGTC Cuts at: 1316 2369 1316 Size: 1053 4435 XbaI T'CTAG_A Cuts at: 2824 2824 Size: 5488 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2257 Size: 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5488 XmaI C'CCGG_G Cuts at: 1870 2556 2813 1870 Size: 686 257 4545 Fragments arranged by size: 4545 686 257 XmnI GAAnn'nnTTC Cuts at: 3617 3617 Size: 5488 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3296 276 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI