Restriction map for
pFN21AB8297
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FseI HaeII
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 178 GACGCC G[GA]^CG[CT]C
AlwNI 73 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 28 CCCGAG C^[CT]CG[GA]G
AviII 413 TGCGCA TGC^GCA
BanII 430 GGGCTC G[GA]GC[CT]^C
BbsI 6 GAAGAC GAAGAC(2/6)
BclI 404 TGATCA T^GATCA
BcnI 246 CCGGG CC^[GC]GG
BglI 113 GCCCCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 458 AGATCT A^GATCT
BpuAI 6 GAAGAC GAAGAC(2/6)
BsaHI 178 GACGCC G[GA]^CG[CT]C
BsoBI 28 CCCGAG C^[CT]CG[GA]G
BspHI 407 TCATGA T^CATGA
BsrI 305 ACTGG ACTGG(1/-1)
BstEII 270 GGTCACC G^GT[ACGT]ACC
BstPI 270 GGTCACC G^GT[ACGT]ACC
BstYI 458 AGATCT [GA]^GATC[CT]
DdeI 59 CTGAG C^T[ACGT]AG
DraII 41 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 279 CCGCGG C^C[GA][CT]GG
EcoO109I 41 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 270 GGTCACC G^GT[ACGT]ACC
FauI 278 CCCGC CCCGC(4/6)
FbaI 404 TGATCA T^GATCA
FspI 413 TGCGCA TGC^GCA
HapII 246 CCGG C^CGG
HgaI 178 GACGC GACGC(5/10)
Hin1I 178 GACGCC G[GA]^CG[CT]C
HinfI 344 GAATC G^A[ACGT]TC
HpaII 246 CCGG C^CGG
HphI 272 TCACC GGTGA(8/7)
KspI 279 CCGCGG CCGC^GG
MflI 458 AGATCT [GA]^GATC[CT]
MspI 246 CCGG C^CGG
NciI 246 CCGGG CC^[GC]GG
NspI 563 ACATGC [GA]CATG^[CT]
PpuMI 41 AGGACCT [GA]G^G[AT]CC[CT]
PstI 553 CTGCAG CTGCA^G
RcaI 407 TCATGA T^CATGA
SacII 279 CCGCGG CCGC^GG
SfcI 553 CTGCAG C^T[GA][CT]AG
TfiI 344 GAATC G^A[AT]TC
XcmI 11 CCAACAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 458 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AfaI 90 GTAC GT^AC
321 GTAC
AvaII 42 GGACC G^G[AT]CC
474 GGACC
BanI 232 GGCACC G^G[CT][GA]CC
397 GGCACC
BmyI 396 GGGCAC G[AGT]GC[ACT]^C
430 GGGCTC
BsaJI 29 CCGAGG C^C[ACGT][ACGT]GG
279 CCGCGG
BseRI 136 GAGGAG GAGGAG(10/8)
505 GAGGAG
BsiYI 126 CCTCAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
477 CCAGGATAAGG
BslI 126 CCTCAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
477 CCAGGATAAGG
Bsp1286I 396 GGGCAC G[AGT]GC[ACT]^C
430 GGGCTC
BstF5I 123 CATCC GGATG(2/0)
536 CATCC
FokI 123 CATCC GGATG(9/13)
536 CATCC
HaeIII 24 GGCC GG^CC
309 GGCC
MspA1I 117 CAGCGG C[AC]G^C[GT]G
279 CCGCGG
RsaI 90 GTAC GT^AC
321 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI BbvI BpmI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI HhaI
HinP1I ItaI MaeIII MboI MboII
MnlI MvaI MvnI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I TspRI
TthHB8I