(Circular) (Six-base) MAPSORT of: pFN21AB8961.seq Check: 2497 from: 1 to: 5731 pFN21AB8961 5731 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2114 2114 Size: 5731 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3074 1937 Size: 1137 4594 Acc65I G'GTAC_C Cuts at: 3052 3052 Size: 5731 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2759 3794 5178 5446 1632 Size: 1127 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1127 1035 268 AfeI AGC'GCT Cuts at: 2840 2840 Size: 5731 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 2895 4630 5607 1203 Size: 1692 1735 977 1327 Fragments arranged by size: 1735 1692 1327 977 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5731 AlwNI CAG_nnn'CTG Cuts at: 3474 5046 3474 Size: 1572 4159 ApaLI G'TGCA_C Cuts at: 3788 4944 3788 Size: 1156 4575 ApoI r'AATT_y Cuts at: 1493 2882 3040 3266 3418 5527 1493 Size: 1389 158 226 152 2109 1697 Fragments arranged by size: 2109 1697 1389 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2353 2466 2562 2588 2907 3056 1870 Size: 73 410 113 96 26 319 149 4545 Fragments arranged by size: 4545 410 319 149 113 96 73 26 BamHI G'GATC_C Cuts at: 2811 3061 3428 2811 Size: 250 367 5114 Fragments arranged by size: 5114 367 250 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2218 2439 3052 4507 Size: 325 217 627 231 200 221 613 1455 Cuts at: 4507 618 Size: 1842 Fragments arranged by size: 1842 1455 627 613 325 231 221 217 200 BanII G_rGCy'C Cuts at: 1391 2310 2700 3050 1391 Size: 919 390 350 4072 Fragments arranged by size: 4072 919 390 350 BbeI G_GCGC'C Cuts at: 2443 2443 Size: 5731 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 928 Size: 971 102 4658 Fragments arranged by size: 4658 971 102 BbvCI CC'TCA_GC Cuts at: 2024 2701 2024 Size: 677 5054 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3922 3956 1903 Size: 34 1985 34 3678 Fragments arranged by size: 3678 1985 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2061 2098 2424 Size: 107 122 71 631 605 389 37 326 Cuts at: 2424 4342 136 Size: 1918 1525 Fragments arranged by size: 1918 1525 631 605 389 326 122 107 71 37 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 3148 3148 Size: 5731 Bme1580I G_kGCm'C Cuts at: 1790 2223 3792 4948 1790 Size: 433 1569 1156 2573 Fragments arranged by size: 2573 1569 1156 433 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2685 3462 4420 Size: 416 53 83 399 24 1268 777 958 Cuts at: 4420 442 Size: 1753 Fragments arranged by size: 1753 1268 958 777 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2182 2182 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2894 4391 1239 Size: 1655 1497 2579 Fragments arranged by size: 2579 1655 1497 Bpu10I CC'TnA_GC Cuts at: 2024 2701 2024 Size: 677 5054 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2888 3856 4721 5019 2888 Size: 968 865 298 3600 Fragments arranged by size: 3600 968 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4394 882 Size: 617 2895 2219 Fragments arranged by size: 2895 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2038 2320 3522 493 Size: 711 834 282 1202 2702 Fragments arranged by size: 2702 1202 834 711 282 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2440 3538 3920 4555 Size: 53 83 186 936 907 1098 382 635 Cuts at: 4555 275 Size: 1451 Fragments arranged by size: 1451 1098 936 907 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4164 4562 4836 4983 5394 4164 Size: 398 274 147 411 4501 Fragments arranged by size: 4501 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2309 2339 2807 2837 1178 Size: 30 165 30 906 30 468 30 4072 Fragments arranged by size: 4072 906 468 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2497 2500 2503 2521 2524 2759 2762 1186 Size: 1311 3 3 18 3 235 3 4155 Fragments arranged by size: 4155 1311 235 18 3 3 3 3 BseYI C'CCAG_C Cuts at: 2226 2408 2420 2428 4934 5430 2226 Size: 182 12 8 2506 496 2527 Fragments arranged by size: 2527 2506 496 182 12 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2145 2193 2314 3181 3942 4970 Size: 275 186 144 48 121 867 761 1028 Cuts at: 4970 1540 Size: 2301 Fragments arranged by size: 2301 1028 867 761 275 186 144 121 48 BsiHKAI G_wGCw'C Cuts at: 2555 3792 3877 4948 2555 Size: 1237 85 1071 3338 Fragments arranged by size: 3338 1237 1071 85 BsiWI C'GTAC_G Cuts at: 2268 2268 Size: 5731 BsmI GAATG_Cn' Cuts at: 2758 3242 3335 2758 Size: 484 93 5154 Fragments arranged by size: 5154 484 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3021 3021 Size: 5731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2310 2555 2700 3050 3792 3877 Size: 399 433 87 245 145 350 742 85 Cuts at: 3877 4948 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 350 245 145 87 85 BspEI T'CCGG_A Cuts at: 5394 5394 Size: 5731 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2114 3086 844 Size: 1270 972 3489 Fragments arranged by size: 3489 1270 972 BsrBI CCG'CTC Cuts at: 1394 1823 2253 2471 3618 1394 Size: 429 430 218 1147 3507 Fragments arranged by size: 3507 1147 430 429 218 BsrDI GCAATG_nn' Cuts at: 65 1248 4226 4400 65 Size: 1183 2978 174 1396 Fragments arranged by size: 2978 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2115 2913 4375 4562 1700 Size: 110 305 798 1462 187 2869 Fragments arranged by size: 2869 1462 798 305 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5731 BssHII G'CGCG_C Cuts at: 1925 2081 2106 1925 Size: 156 25 5550 Fragments arranged by size: 5550 156 25 BssSI C'ACGA_G Cuts at: 2167 2206 2596 3791 4803 2167 Size: 39 390 1195 1012 3095 Fragments arranged by size: 3095 1195 1012 390 39 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5731 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5731 BstYI r'GATC_y Cuts at: 1972 2811 3061 3428 3813 3830 5282 5368 5380 Size: 839 250 367 385 17 1452 86 12 Cuts at: 5380 5726 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 839 385 367 346 250 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2214 2401 2487 2556 513 Size: 552 1149 187 86 69 3688 Fragments arranged by size: 3688 1149 552 187 86 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2261 487 Size: 683 56 1035 3957 Fragments arranged by size: 3957 1035 683 56 BtsI GCAGTG_nn' Cuts at: 3247 4041 4061 3247 Size: 794 20 4917 Fragments arranged by size: 4917 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3035 3035 Size: 5731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4738 809 Size: 507 3422 1802 Fragments arranged by size: 3422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2190 2289 Size: 54 1425 50 275 18 312 48 99 Cuts at: 2289 2311 2928 3178 4067 5696 8 Size: 22 617 250 889 1629 43 Fragments arranged by size: 1629 1425 889 617 312 275 250 99 54 50 48 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2142 2190 2311 3178 1537 Size: 275 330 48 121 867 4090 Fragments arranged by size: 4090 867 330 275 121 48 EarI CTCTTCn'nnn_ Cuts at: 1353 2894 3661 1353 Size: 1541 767 3423 Fragments arranged by size: 3423 1541 767 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4318 4692 4838 387 Size: 1064 138 2729 374 146 1280 Fragments arranged by size: 2729 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2759 2894 3794 4391 5178 5446 1239 Size: 393 1127 135 900 597 787 268 1524 Fragments arranged by size: 1524 1127 900 787 597 393 268 135 EcoNI CCTnn'n_nnAGG Cuts at: 2655 2655 Size: 5731 EcoRI G'AATT_C Cuts at: 2882 3040 3418 2882 Size: 158 378 5195 Fragments arranged by size: 5195 378 158 FseI GG_CCGG'CC Cuts at: 2119 2119 Size: 5731 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 2443 2842 2920 4878 1678 Size: 765 399 78 1958 2531 Fragments arranged by size: 2531 1958 765 399 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2803 2835 4372 4404 4446 Size: 32 135 32 1231 32 1537 32 42 Cuts at: 4446 4478 1373 Size: 32 2626 Fragments arranged by size: 2626 1537 1231 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5731 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KasI G'GCGC_C Cuts at: 2439 2439 Size: 5731 KpnI G_GTAC'C Cuts at: 3056 3056 Size: 5731 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2673 4845 5029 1305 Size: 1368 2172 184 2007 Fragments arranged by size: 2172 2007 1368 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2930 10 Size: 54 1425 343 1098 2811 Fragments arranged by size: 2811 1425 1098 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3689 4048 4207 518 Size: 648 2523 359 159 2042 Fragments arranged by size: 2523 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2216 2240 2341 2403 2424 2583 2630 3147 Size: 610 24 101 62 21 159 47 517 Cuts at: 3147 3824 4972 5217 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 610 517 245 159 101 62 47 24 21 NaeI GCC'GGC Cuts at: 2117 2117 Size: 5731 NarI GG'CG_CC Cuts at: 2440 2440 Size: 5731 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5179 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NgoMIV G'CCGG_C Cuts at: 2115 2115 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 2899 3089 4634 1132 Size: 97 1670 190 1545 2229 Fragments arranged by size: 2229 1670 1545 190 97 PasI CC'CwG_GG Cuts at: 1348 2572 1348 Size: 1224 4507 PciI A'CATG_T Cuts at: 2895 4630 2895 Size: 1735 3996 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5731 PfoI T'CCnGG_A Cuts at: 2327 2327 Size: 5731 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PstI C_TGCA'G Cuts at: 830 2125 2616 3083 830 Size: 1295 491 467 3478 Fragments arranged by size: 3478 1295 491 467 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 RsrII CG'GwC_CG Cuts at: 2393 2393 Size: 5731 SacII CC_GC'GG Cuts at: 2217 2404 2217 Size: 187 5544 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SbfI CC_TGCA'GG Cuts at: 2125 2616 3083 2125 Size: 491 467 4773 Fragments arranged by size: 4773 491 467 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 2121 2612 3079 4214 4895 5086 826 Size: 219 1076 491 467 1135 681 191 1471 Fragments arranged by size: 1471 1135 1076 681 491 467 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2098 2424 2098 Size: 326 5405 SfoI GGC'GCC Cuts at: 2441 2441 Size: 5731 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 2355 2564 2909 3058 1872 Size: 483 209 345 149 4545 Fragments arranged by size: 4545 483 345 209 149 SmlI C'TyrA_G Cuts at: 820 1017 1943 2867 3835 4736 4998 820 Size: 197 926 924 968 901 262 1553 Fragments arranged by size: 1553 968 926 924 901 262 197 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 3089 1132 Size: 1957 3774 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5731 StyI C'CwwG_G Cuts at: 513 1065 3170 513 Size: 552 2105 3074 Fragments arranged by size: 3074 2105 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3758 3775 3928 4113 4558 325 Size: 1255 2178 17 153 185 445 1498 Fragments arranged by size: 2178 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3977 5689 96 Size: 274 80 33 51 1443 2000 1712 138 Fragments arranged by size: 2000 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2807 2839 5691 5723 2807 Size: 32 2852 32 2815 Fragments arranged by size: 2852 2815 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 2353 2562 2907 3056 1870 Size: 483 209 345 149 4545 Fragments arranged by size: 4545 483 345 209 149 XmnI GAAnn'nnTTC Cuts at: 2882 3860 2882 Size: 978 4753 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SanDI SapI SexAI SrfI SwaI XcmI