(Circular) (Six-base) MAPSORT of: pFN21AB6495.seq Check: 8101 from: 1 to: 5560 pFN21AB6495 5560 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3370 278 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2903 1937 Size: 966 4594 Acc65I G'GTAC_C Cuts at: 2881 2881 Size: 5560 AclI AA'CG_TT Cuts at: 1563 3687 4060 1563 Size: 2124 373 3063 Fragments arranged by size: 3063 2124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2514 3623 5007 5275 1632 Size: 882 1109 1384 268 1917 Fragments arranged by size: 1917 1384 1109 882 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5363 AflIII A'CryG_T Cuts at: 1203 4459 5436 1203 Size: 3256 977 1327 Fragments arranged by size: 3256 1327 977 AgeI A'CCGG_T Cuts at: 4391 4391 Size: 5560 AhdI GACnn_n'nnGTC Cuts at: 4289 4289 Size: 5560 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5560 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2633 2665 2633 Size: 32 5528 AlwNI CAG_nnn'CTG Cuts at: 3303 4875 3303 Size: 1572 3988 ApaLI G'TGCA_C Cuts at: 3617 4773 3617 Size: 1156 4404 ApoI r'AATT_y Cuts at: 1493 2279 2869 3095 3247 5356 1493 Size: 786 590 226 152 2109 1697 Fragments arranged by size: 2109 1697 786 590 226 152 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2146 2885 1870 Size: 73 203 739 4545 Fragments arranged by size: 4545 739 203 73 BamHI G'GATC_C Cuts at: 2314 2566 2890 3257 2314 Size: 252 324 367 4617 Fragments arranged by size: 4617 367 324 252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2108 2140 2165 2881 Size: 325 217 627 258 63 32 25 716 Cuts at: 2881 4336 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 716 627 325 258 217 63 32 25 BanII G_rGCy'C Cuts at: 1391 2879 1391 Size: 1488 4072 BbeI G_GCGC'C Cuts at: 2049 2112 2144 2169 2049 Size: 63 32 25 5440 Fragments arranged by size: 5440 63 32 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3751 3785 1903 Size: 34 1814 34 3678 Fragments arranged by size: 3678 1814 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2236 3452 4662 2236 Size: 1216 1210 3134 Fragments arranged by size: 3134 1216 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4171 136 Size: 107 122 71 631 605 2499 1525 Fragments arranged by size: 2499 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5555 5555 Size: 5560 BlpI GC'TnA_GC Cuts at: 2977 2977 Size: 5560 Bme1580I G_kGCm'C Cuts at: 1790 3621 4777 1790 Size: 1831 1156 2573 Fragments arranged by size: 2573 1831 1156 BmgBI CAC'GTC Cuts at: 1318 2828 1318 Size: 1510 4050 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 4249 442 Size: 416 53 83 399 24 1874 958 1753 Fragments arranged by size: 1874 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2145 2660 4220 1239 Size: 906 515 1560 2579 Fragments arranged by size: 2579 1560 906 515 Bpu10I CC'TnA_GC Cuts at: 2234 2234 Size: 5560 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3685 4550 4848 3685 Size: 865 298 4397 Fragments arranged by size: 4397 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4223 882 Size: 617 2724 2219 Fragments arranged by size: 2724 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3351 493 Size: 711 2147 2702 Fragments arranged by size: 2702 2147 711 BsaBI GATnn'nnATC Cuts at: 5554 5554 Size: 5560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2109 2141 2166 Size: 53 83 186 936 513 63 32 25 Cuts at: 2166 3367 3749 4384 275 Size: 1201 382 635 1451 Fragments arranged by size: 1451 1201 936 635 513 382 186 83 63 53 32 25 BsaWI w'CCGG_w Cuts at: 3993 4391 4665 4812 5223 3993 Size: 398 274 147 411 4330 Fragments arranged by size: 4330 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2277 2307 2633 2663 1178 Size: 30 165 30 874 30 326 30 4075 Fragments arranged by size: 4075 874 326 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2216 1186 Size: 850 180 4530 Fragments arranged by size: 4530 850 180 BseYI C'CCAG_C Cuts at: 2238 2691 4763 5259 2238 Size: 453 2072 496 2539 Fragments arranged by size: 2539 2072 496 453 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4987 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 2136 3010 3771 4799 1540 Size: 275 186 93 42 874 761 1028 2301 Fragments arranged by size: 2301 1028 874 761 275 186 93 42 BsiHKAI G_wGCw'C Cuts at: 2403 3621 3706 4777 2403 Size: 1218 85 1071 3186 Fragments arranged by size: 3186 1218 1071 85 BsmI GAATG_Cn' Cuts at: 3071 3164 3071 Size: 93 5467 BsmBI CGTCTCn'nnnn_ Cuts at: 2030 2030 Size: 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2403 2879 3621 3706 4777 1391 Size: 399 613 476 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 613 476 399 85 BspEI T'CCGG_A Cuts at: 5223 5223 Size: 5560 BspHI T'CATG_A Cuts at: 3449 5179 3449 Size: 1730 3830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2915 844 Size: 2071 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2151 3447 1394 Size: 429 328 1296 3507 Fragments arranged by size: 3507 1296 429 328 BsrDI GCAATG_nn' Cuts at: 65 1248 4055 4229 65 Size: 1183 2807 174 1396 Fragments arranged by size: 2807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2089 4204 4391 1700 Size: 110 279 2115 187 2869 Fragments arranged by size: 2869 2115 279 187 110 BsrGI T'GTAC_A Cuts at: 96 2300 96 Size: 2204 3356 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5560 BssSI C'ACGA_G Cuts at: 3620 4632 3620 Size: 1012 4548 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5560 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstXI CCAn_nnnn'nTGG Cuts at: 2828 2828 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2314 2566 2890 3257 3642 3659 5111 5197 Size: 342 252 324 367 385 17 1452 86 Cuts at: 5197 5209 5555 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 342 324 252 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2031 2112 2136 2439 2737 513 Size: 552 966 81 24 303 298 3336 Fragments arranged by size: 3336 966 552 303 298 81 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4821 Fragments arranged by size: 4821 683 56 BtsI GCAGTG_nn' Cuts at: 3076 3870 3890 3076 Size: 794 20 4746 Fragments arranged by size: 4746 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2262 2611 2864 2262 Size: 349 253 4958 Fragments arranged by size: 4958 349 253 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4567 809 Size: 507 3251 1802 Fragments arranged by size: 3251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2413 2833 Size: 54 1425 50 275 18 261 322 420 Cuts at: 2833 3007 3896 5525 8 Size: 174 889 1629 43 Fragments arranged by size: 1629 1425 889 420 322 275 261 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2091 3007 1537 Size: 275 279 916 4090 Fragments arranged by size: 4090 916 279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3490 1353 Size: 2137 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4147 4521 4667 387 Size: 1064 138 2558 374 146 1280 Fragments arranged by size: 2558 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2145 2514 2660 3623 4220 5007 5275 Size: 393 513 369 146 963 597 787 268 Cuts at: 5275 1239 Size: 1524 Fragments arranged by size: 1524 963 787 597 513 393 369 268 146 EcoO109I rG'GnC_Cy Cuts at: 2383 2455 2383 Size: 72 5488 EcoRI G'AATT_C Cuts at: 2869 3247 2869 Size: 378 5182 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2488 2488 Size: 5560 FspI TGC'GCA Cuts at: 4066 4066 Size: 5560 HaeII r_GCGC'y Cuts at: 1678 2049 2112 2144 2169 4707 1678 Size: 371 63 32 25 2538 2531 Fragments arranged by size: 2538 2531 371 63 32 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2140 2172 4201 4233 4275 Size: 32 135 32 568 32 2029 32 42 Cuts at: 4275 4307 1373 Size: 32 2626 Fragments arranged by size: 2626 2029 568 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2296 2904 3150 669 Size: 988 281 358 608 246 3079 Fragments arranged by size: 3079 988 608 358 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5560 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KasI G'GCGC_C Cuts at: 2045 2108 2140 2165 2045 Size: 63 32 25 5440 Fragments arranged by size: 5440 63 32 25 KpnI G_GTAC'C Cuts at: 2885 2885 Size: 5560 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 4674 4858 1305 Size: 684 2685 184 2007 Fragments arranged by size: 2685 2007 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2835 10 Size: 54 1425 343 1003 2735 Fragments arranged by size: 2735 1425 1003 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 3518 3877 4036 518 Size: 648 1278 1074 359 159 2042 Fragments arranged by size: 2042 1278 1074 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2114 2138 2769 2976 3653 4801 5046 1606 Size: 508 24 631 207 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 631 508 245 207 24 NaeI GCC'GGC Cuts at: 2091 2091 Size: 5560 NarI GG'CG_CC Cuts at: 2046 2109 2141 2166 2046 Size: 63 32 25 5440 Fragments arranged by size: 5440 63 32 25 NcoI C'CATG_G Cuts at: 513 1065 2031 2439 513 Size: 552 966 408 3634 Fragments arranged by size: 3634 966 552 408 NdeI CA'TA_TG Cuts at: 387 387 Size: 5560 NgoMIV G'CCGG_C Cuts at: 2089 2089 Size: 5560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5560 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NspI r_CATG'y Cuts at: 1132 1229 2918 4463 1132 Size: 97 1689 1545 2229 Fragments arranged by size: 2229 1689 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5560 PciI A'CATG_T Cuts at: 4459 4459 Size: 5560 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5560 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2633 2665 3629 3661 5142 5174 2633 Size: 32 964 32 1481 32 3019 Fragments arranged by size: 3019 1481 964 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2383 2455 2383 Size: 72 5488 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 3130 3130 Size: 5560 PstI C_TGCA'G Cuts at: 830 2912 830 Size: 2082 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 SacII CC_GC'GG Cuts at: 2115 2139 2115 Size: 24 5536 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SbfI CC_TGCA'GG Cuts at: 2912 2912 Size: 5560 ScaI AGT'ACT Cuts at: 2522 3808 5520 2522 Size: 1286 1712 2562 Fragments arranged by size: 2562 1712 1286 SfcI C'TryA_G Cuts at: 826 1045 2908 4043 4724 4915 826 Size: 219 1863 1135 681 191 1471 Fragments arranged by size: 1863 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2047 2110 2142 2167 2047 Size: 63 32 25 5440 Fragments arranged by size: 5440 63 32 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3664 4565 4827 820 Size: 197 926 1721 901 262 1553 Fragments arranged by size: 1721 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5560 SphI G_CATG'C Cuts at: 1132 2918 1132 Size: 1786 3774 SspI AAT'ATT Cuts at: 5 52 3484 5 Size: 47 3432 2081 Fragments arranged by size: 3432 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5560 StyI C'CwwG_G Cuts at: 513 1065 2031 2439 2999 513 Size: 552 966 408 560 3074 Fragments arranged by size: 3074 966 560 552 408 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3587 3604 3757 3942 4387 325 Size: 1255 2007 17 153 185 445 1498 Fragments arranged by size: 2007 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2300 2520 2801 Size: 274 80 33 51 1443 323 220 281 Cuts at: 2801 3806 5518 96 Size: 1005 1712 138 Fragments arranged by size: 1712 1443 1005 323 281 274 220 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5520 5552 5520 Size: 32 5528 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5560 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2660 2828 2660 Size: 168 5392 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 2471 2648 3689 2471 Size: 177 1041 4342 Fragments arranged by size: 4342 1041 177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3368 276 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI