Restriction map for
pFN21AB6495
(Linear) (Six-base) MAPSORT of: KIBB6495.seq Check: 8882 from: 1 to: 855
KIBB6495.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 855
Size: 509 346
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 628 660 855
Size: 628 32 195
Fragments arranged by size:
628 195 32
ApoI r'AATT_y
Cuts at: 0 274 855
Size: 274 581
AvaI C'yCGr_G
Cuts at: 0 141 855
Size: 141 714
BamHI G'GATC_C
Cuts at: 0 309 561 855
Size: 309 252 294
Fragments arranged by size:
309 294 252
BanI G'GyrC_C
Cuts at: 0 40 103 135 160 855
Size: 40 63 32 25 695
Fragments arranged by size:
695 63 40 32 25
BbeI G_GCGC'C
Cuts at: 0 44 107 139 164 855
Size: 44 63 32 25 691
Fragments arranged by size:
691 63 44 32 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 231 855
Size: 231 624
BmgBI CAC'GTC
Cuts at: 0 823 855
Size: 823 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 655 855
Size: 140 515 200
Fragments arranged by size:
515 200 140
Bpu10I CC'TnA_GC
Cuts at: 0 229 855
Size: 229 626
BsaHI Gr'CG_yC
Cuts at: 0 41 104 136 161 855
Size: 41 63 32 25 694
Fragments arranged by size:
694 63 41 32 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 272 302 628 658 855
Size: 272 30 326 30 197
Fragments arranged by size:
326 272 197 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 211 855
Size: 31 180 644
Fragments arranged by size:
644 180 31
BseYI C'CCAG_C
Cuts at: 0 233 686 855
Size: 233 453 169
Fragments arranged by size:
453 233 169
BsiEI CG_ry'CG
Cuts at: 0 89 131 855
Size: 89 42 724
Fragments arranged by size:
724 89 42
BsiHKAI G_wGCw'C
Cuts at: 0 398 855
Size: 398 457
BsmBI CGTCTCn'nnnn_
Cuts at: 0 25 855
Size: 25 830
Bsp1286I G_dGCh'C
Cuts at: 0 398 855
Size: 398 457
BsrBI CCG'CTC
Cuts at: 0 146 855
Size: 146 709
BsrFI r'CCGG_y
Cuts at: 0 84 855
Size: 84 771
BsrGI T'GTAC_A
Cuts at: 0 295 855
Size: 295 560
BstXI CCAn_nnnn'nTGG
Cuts at: 0 823 855
Size: 823 32
BstYI r'GATC_y
Cuts at: 0 309 561 855
Size: 309 252 294
Fragments arranged by size:
309 294 252
BtgI C'CryG_G
Cuts at: 0 26 107 131 434 732 855
Size: 26 81 24 303 298 123
Fragments arranged by size:
303 298 123 81 26 24
DraI TTT'AAA
Cuts at: 0 257 606 855
Size: 257 349 249
Fragments arranged by size:
349 257 249
EaeI y'GGCC_r
Cuts at: 0 86 408 828 855
Size: 86 322 420 27
Fragments arranged by size:
420 322 86 27
EagI C'GGCC_G
Cuts at: 0 86 855
Size: 86 769
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 509 655 855
Size: 140 369 146 200
Fragments arranged by size:
369 200 146 140
EcoO109I rG'GnC_Cy
Cuts at: 0 378 450 855
Size: 378 72 405
Fragments arranged by size:
405 378 72
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 483 855
Size: 483 372
HaeII r_GCGC'y
Cuts at: 0 44 107 139 164 855
Size: 44 63 32 25 691
Fragments arranged by size:
691 63 44 32 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 855
Size: 135 32 688
Fragments arranged by size:
688 135 32
HincII GTy'rAC
Cuts at: 0 291 855
Size: 291 564
KasI G'GCGC_C
Cuts at: 0 40 103 135 160 855
Size: 40 63 32 25 695
Fragments arranged by size:
695 63 40 32 25
MscI TGG'CCA
Cuts at: 0 830 855
Size: 830 25
MslI CAynn'nnrTG
Cuts at: 0 439 855
Size: 439 416
MspA1I CmG'CkG
Cuts at: 0 109 133 764 855
Size: 109 24 631 91
Fragments arranged by size:
631 109 91 24
NaeI GCC'GGC
Cuts at: 0 86 855
Size: 86 769
NarI GG'CG_CC
Cuts at: 0 41 104 136 161 855
Size: 41 63 32 25 694
Fragments arranged by size:
694 63 41 32 25
NcoI C'CATG_G
Cuts at: 0 26 434 855
Size: 26 408 421
Fragments arranged by size:
421 408 26
NgoMIV G'CCGG_C
Cuts at: 0 84 855
Size: 84 771
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 628 660 855
Size: 628 32 195
Fragments arranged by size:
628 195 32
PpuMI rG'GwC_Cy
Cuts at: 0 378 450 855
Size: 378 72 405
Fragments arranged by size:
405 378 72
SacII CC_GC'GG
Cuts at: 0 110 134 855
Size: 110 24 721
Fragments arranged by size:
721 110 24
ScaI AGT'ACT
Cuts at: 0 517 855
Size: 517 338
SfoI GGC'GCC
Cuts at: 0 42 105 137 162 855
Size: 42 63 32 25 693
Fragments arranged by size:
693 63 42 32 25
StyI C'CwwG_G
Cuts at: 0 26 434 855
Size: 26 408 421
Fragments arranged by size:
421 408 26
TatI w'GTAC_w
Cuts at: 0 295 515 796 855
Size: 295 220 281 59
Fragments arranged by size:
295 281 220 59
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 655 823 855
Size: 655 168 32
Fragments arranged by size:
655 168 32
XmnI GAAnn'nnTTC
Cuts at: 0 466 643 855
Size: 466 177 212
Fragments arranged by size:
466 212 177
Enzymes that do cut:
AcuI AloI ApoI AvaI BamHI BanI BbeI BciVI
BmgBI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BsrGI BstXI BstYI
BtgI DraI EaeI EagI Eco57MI EcoO109I FalI HaeII
Hin4I HincII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PpiI PpuMI SacII ScaI SfoI StyI
TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BanII BbsI BbvCI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiWI
BsmI BspEI BspHI BspMI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
ZraI