Restriction map for
pFN21AB6353
(Linear) (Six-base) MAPSORT of: KIBB6353.seq Check: 670 from: 1 to: 798
KIBB6353.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 610 798
Size: 610 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 798
Size: 31 767
AlwNI CAG_nnn'CTG
Cuts at: 0 411 558 798
Size: 411 147 240
Fragments arranged by size:
411 240 147
AscI GG'CGCG_CC
Cuts at: 0 300 798
Size: 300 498
BanII G_rGCy'C
Cuts at: 0 70 634 798
Size: 70 564 164
Fragments arranged by size:
564 164 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 299 798
Size: 299 499
BciVI GTATCCnnnnn_n'
Cuts at: 0 401 798
Size: 401 397
BlpI GC'TnA_GC
Cuts at: 0 64 798
Size: 64 734
Bme1580I G_kGCm'C
Cuts at: 0 767 798
Size: 767 31
BmgBI CAC'GTC
Cuts at: 0 216 798
Size: 216 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 798
Size: 41 757
BsaWI w'CCGG_w
Cuts at: 0 482 798
Size: 482 316
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 798
Size: 205 593
BseYI C'CCAG_C
Cuts at: 0 85 243 505 708 798
Size: 85 158 262 203 90
Fragments arranged by size:
262 203 158 90 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 379 653 798
Size: 151 228 274 145
Fragments arranged by size:
274 228 151 145
BsiHKAI G_wGCw'C
Cuts at: 0 70 617 657 798
Size: 70 547 40 141
Fragments arranged by size:
547 141 70 40
Bsp1286I G_dGCh'C
Cuts at: 0 70 617 634 657 767 798
Size: 70 547 17 23 110 31
Fragments arranged by size:
547 110 70 31 23 17
BspEI T'CCGG_A
Cuts at: 0 482 798
Size: 482 316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 610 798
Size: 610 188
BsrBI CCG'CTC
Cuts at: 0 153 798
Size: 153 645
BssHII G'CGCG_C
Cuts at: 0 300 798
Size: 300 498
BssSI C'ACGA_G
Cuts at: 0 790 798
Size: 790 8
Bsu36I CC'TnA_GG
Cuts at: 0 377 798
Size: 377 421
BtgI C'CryG_G
Cuts at: 0 305 798
Size: 305 493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 62 89 97 124 798
Size: 62 27 8 27 674
Fragments arranged by size:
674 62 27 27 8
EarI CTCTTCn'nnn_
Cuts at: 0 491 724 798
Size: 491 233 74
Fragments arranged by size:
491 233 74
EcoICRI GAG'CTC
Cuts at: 0 68 798
Size: 68 730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 41 798
Size: 31 10 757
Fragments arranged by size:
757 31 10
EcoO109I rG'GnC_Cy
Cuts at: 0 401 798
Size: 401 397
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 375 407 618 650 798
Size: 375 32 211 32 148
Fragments arranged by size:
375 211 148 32 32
MspA1I CmG'CkG
Cuts at: 0 307 363 798
Size: 307 56 435
Fragments arranged by size:
435 307 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 558 798
Size: 558 240
PfoI T'CCnGG_A
Cuts at: 0 287 798
Size: 287 511
PpuMI rG'GwC_Cy
Cuts at: 0 401 798
Size: 401 397
PstI C_TGCA'G
Cuts at: 0 325 416 798
Size: 325 91 382
Fragments arranged by size:
382 325 91
SacI G_AGCT'C
Cuts at: 0 70 798
Size: 70 728
SacII CC_GC'GG
Cuts at: 0 308 798
Size: 308 490
SapI GCTCTTCn'nnn_
Cuts at: 0 724 798
Size: 724 74
ScaI AGT'ACT
Cuts at: 0 265 798
Size: 265 533
SfcI C'TryA_G
Cuts at: 0 321 412 798
Size: 321 91 386
Fragments arranged by size:
386 321 91
StuI AGG'CCT
Cuts at: 0 524 798
Size: 524 274
StyI C'CwwG_G
Cuts at: 0 723 798
Size: 723 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 632 794 798
Size: 632 162 4
Fragments arranged by size:
632 162 4
TatI w'GTAC_w
Cuts at: 0 263 798
Size: 263 535
XmnI GAAnn'nnTTC
Cuts at: 0 283 448 575 798
Size: 283 165 127 223
Fragments arranged by size:
283 223 165 127
Enzymes that do cut:
AarI AcuI AlwNI AscI BanII BbsI BciVI BlpI
Bme1580I BmgBI BpmI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BssHII BssSI Bsu36I BtgI
CspCI EarI EcoICRI Eco57MI EcoO109I Hin4I MspA1I PflMI
PfoI PpuMI PstI SacI SacII SapI ScaI SfcI
StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BfrBI BglI BglII BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI
BsiWI BsmI BsmBI BspHI BsrDI BsrFI BsrGI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI