(Circular) (Six-base) MAPSORT of: pFN21AB7326.seq Check: 1016 from: 1 to: 8350 pFN21AB7326 8350 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2261 5602 2261 Size: 3341 5009 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6160 278 Size: 53 83 186 936 4624 2468 Fragments arranged by size: 4624 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2025 5693 1937 Size: 88 3668 4594 Fragments arranged by size: 4594 3668 88 Acc65I G'GTAC_C Cuts at: 5671 5671 Size: 8350 AclI AA'CG_TT Cuts at: 1563 6477 6850 1563 Size: 4914 373 3063 Fragments arranged by size: 4914 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5152 6413 7797 8065 1632 Size: 3520 1261 1384 268 1917 Fragments arranged by size: 3520 1917 1384 1261 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8153 AflIII A'CryG_T Cuts at: 1203 3147 4255 7249 8226 1203 Size: 1944 1108 2994 977 1327 Fragments arranged by size: 2994 1944 1327 1108 977 AgeI A'CCGG_T Cuts at: 7181 7181 Size: 8350 AhdI GACnn_n'nnGTC Cuts at: 3040 3151 7079 3040 Size: 111 3928 4311 Fragments arranged by size: 4311 3928 111 AleI CACnn'nnGTG Cuts at: 1166 4910 5306 1166 Size: 3744 396 4210 Fragments arranged by size: 4210 3744 396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2262 2441 4525 1184 Size: 1078 179 2084 5009 Fragments arranged by size: 5009 2084 1078 179 AlwNI CAG_nnn'CTG Cuts at: 3836 4051 6093 7665 3836 Size: 215 2042 1572 4521 Fragments arranged by size: 4521 2042 1572 215 ApaLI G'TGCA_C Cuts at: 3806 6407 7563 3806 Size: 2601 1156 4593 Fragments arranged by size: 4593 2601 1156 ApoI r'AATT_y Cuts at: 1493 2158 2682 2873 3494 3664 3745 5659 5885 Size: 665 524 191 621 170 81 1914 226 Cuts at: 5885 6037 8146 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1914 1697 665 621 524 226 191 170 152 81 AseI AT'TA_AT Cuts at: 160 1032 4434 5066 6904 160 Size: 872 3402 632 1838 1606 Fragments arranged by size: 3402 1838 1606 872 632 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8350 AvaI C'yCGr_G Cuts at: 1870 1943 2217 4591 5675 1870 Size: 73 274 2374 1084 4545 Fragments arranged by size: 4545 2374 1084 274 73 BamHI G'GATC_C Cuts at: 2998 5680 6047 2998 Size: 2682 367 5301 Fragments arranged by size: 5301 2682 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2320 4054 4990 5671 7126 Size: 325 217 627 533 1734 936 681 1455 Cuts at: 7126 618 Size: 1842 Fragments arranged by size: 1842 1734 1455 936 681 627 533 325 217 BanII G_rGCy'C Cuts at: 1391 3106 5669 1391 Size: 1715 2563 4072 Fragments arranged by size: 4072 2563 1715 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5236 928 Size: 971 3337 4042 Fragments arranged by size: 4042 3337 971 BbvCI CC'TCA_GC Cuts at: 4230 4269 4230 Size: 39 8311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6541 6575 1903 Size: 34 4604 34 3678 Fragments arranged by size: 4604 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4954 5649 6242 7452 4954 Size: 695 593 1210 5852 Fragments arranged by size: 5852 1210 695 593 BclI T'GATC_A Cuts at: 1548 3220 1548 Size: 1672 6678 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4063 5321 6961 Size: 107 122 71 631 605 2391 1258 1640 Cuts at: 6961 136 Size: 1525 Fragments arranged by size: 2391 1640 1525 1258 631 605 122 107 71 BglII A'GATC_T Cuts at: 8345 8345 Size: 8350 BlpI GC'TnA_GC Cuts at: 5767 5767 Size: 8350 Bme1580I G_kGCm'C Cuts at: 1790 3810 5226 6411 7567 1790 Size: 2020 1416 1185 1156 2573 Fragments arranged by size: 2573 2020 1416 1185 1156 BmgBI CAC'GTC Cuts at: 1318 4026 1318 Size: 2708 5642 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4153 5469 5492 Size: 416 53 83 399 24 2736 1316 23 Cuts at: 5492 6081 7039 442 Size: 589 958 1753 Fragments arranged by size: 2736 1753 1316 958 589 416 399 83 53 24 23 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2556 3090 3534 7010 1239 Size: 1317 534 444 3476 2579 Fragments arranged by size: 3476 2579 1317 534 444 Bpu10I CC'TnA_GC Cuts at: 2295 4230 4269 2295 Size: 1935 39 6376 Fragments arranged by size: 6376 1935 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2957 3231 5416 6475 7340 7638 2957 Size: 274 2185 1059 865 298 3669 Fragments arranged by size: 3669 2185 1059 865 298 274 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3551 4795 7013 882 Size: 617 2052 1244 2218 2219 Fragments arranged by size: 2219 2218 2052 1244 617 BsaAI yAC'GTr Cuts at: 493 1204 2314 6141 493 Size: 711 1110 3827 2702 Fragments arranged by size: 3827 2702 1110 711 BsaBI GATnn'nnATC Cuts at: 8344 8344 Size: 8350 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6157 6539 7174 275 Size: 53 83 186 936 4624 382 635 1451 Fragments arranged by size: 4624 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6783 7181 7455 7602 8013 6783 Size: 398 274 147 411 7120 Fragments arranged by size: 7120 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8125 Fragments arranged by size: 8125 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 4759 1186 Size: 1228 2345 4777 Fragments arranged by size: 4777 2345 1228 BseYI C'CCAG_C Cuts at: 2370 3889 3979 7553 8049 2370 Size: 1519 90 3574 496 2671 Fragments arranged by size: 3574 2671 1519 496 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2336 2913 4007 5379 1163 Size: 573 600 577 1094 1372 4134 Fragments arranged by size: 4134 1372 1094 600 577 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5800 6561 7589 1540 Size: 275 186 3799 761 1028 2301 Fragments arranged by size: 3799 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3106 3810 4709 4907 6411 6496 7567 3106 Size: 704 899 198 1504 85 1071 3889 Fragments arranged by size: 3889 1504 1071 899 704 198 85 BsmI GAATG_Cn' Cuts at: 2798 5144 5861 5954 2798 Size: 2346 717 93 5194 Fragments arranged by size: 5194 2346 717 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3106 3810 4709 4907 5226 5669 6411 Size: 399 1316 704 899 198 319 443 742 Cuts at: 6411 6496 7567 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1316 1071 899 742 704 443 399 319 198 85 BspEI T'CCGG_A Cuts at: 8013 8013 Size: 8350 BspHI T'CATG_A Cuts at: 4924 6239 7969 4924 Size: 1315 1730 5305 Fragments arranged by size: 5305 1730 1315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2261 5602 5705 844 Size: 1417 3341 103 3489 Fragments arranged by size: 3489 3341 1417 103 BsrBI CCG'CTC Cuts at: 1394 1823 3196 6237 1394 Size: 429 1373 3041 3507 Fragments arranged by size: 3507 3041 1373 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2234 2430 2466 5580 6845 7019 65 Size: 1183 986 196 36 3114 1265 174 1396 Fragments arranged by size: 3114 1396 1265 1183 986 196 174 36 BsrFI r'CCGG_y Cuts at: 1700 1810 6994 7181 1700 Size: 110 5184 187 2869 Fragments arranged by size: 5184 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8350 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8350 BssSI C'ACGA_G Cuts at: 6410 7422 6410 Size: 1012 7338 BstAPI GCAn_nnn'nTGC Cuts at: 794 3836 5312 5594 794 Size: 3042 1476 282 3550 Fragments arranged by size: 3550 3042 1476 282 BstBI TT'CG_AA Cuts at: 7154 7154 Size: 8350 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8350 BstXI CCAn_nnnn'nTGG Cuts at: 2378 5324 5477 2378 Size: 2946 153 5251 Fragments arranged by size: 5251 2946 153 BstYI r'GATC_y Cuts at: 1972 2998 3999 5189 5680 6047 6432 6449 7901 Size: 1026 1001 1190 491 367 385 17 1452 Cuts at: 7901 7987 7999 8345 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1190 1026 1001 491 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4771 4771 Size: 8350 BtgI C'CryG_G Cuts at: 513 1065 2004 2126 3811 513 Size: 552 939 122 1685 5052 Fragments arranged by size: 5052 1685 939 552 122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2389 487 Size: 683 56 1163 6448 Fragments arranged by size: 6448 1163 683 56 BtsI GCAGTG_nn' Cuts at: 2265 2925 4608 4630 5118 5313 5866 6660 6680 Size: 660 1683 22 488 195 553 794 20 Cuts at: 6680 2265 Size: 3935 Fragments arranged by size: 3935 1683 794 660 553 488 195 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8315 DraI TTT'AAA Cuts at: 5654 5654 Size: 8350 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7357 809 Size: 507 6041 1802 Fragments arranged by size: 6041 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2629 2922 3045 Size: 54 1425 50 275 18 799 293 123 Cuts at: 3045 3191 5797 6686 8315 8 Size: 146 2606 889 1629 43 Fragments arranged by size: 2606 1629 1425 889 799 293 275 146 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5797 1537 Size: 275 3985 4090 Fragments arranged by size: 4090 3985 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3348 3484 4203 4342 4635 4818 4879 6280 Size: 1995 136 719 139 293 183 61 1401 Cuts at: 6280 1353 Size: 3423 Fragments arranged by size: 3423 1995 1401 719 293 183 139 136 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6937 7311 7457 387 Size: 1064 138 5348 374 146 1280 Fragments arranged by size: 5348 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3104 3104 Size: 8350 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2556 3090 3534 5152 6413 7010 7797 Size: 393 924 534 444 1618 1261 597 787 Cuts at: 7797 8065 1239 Size: 268 1524 Fragments arranged by size: 1618 1524 1261 924 787 597 534 444 393 268 EcoO109I rG'GnC_Cy Cuts at: 2366 3976 2366 Size: 1610 6740 EcoRI G'AATT_C Cuts at: 5659 6037 5659 Size: 378 7972 FspI TGC'GCA Cuts at: 6856 6856 Size: 8350 HaeII r_GCGC'y Cuts at: 1678 7497 1678 Size: 5819 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 3026 3058 3137 Size: 32 135 32 857 32 565 32 79 Cuts at: 3137 3169 3180 3212 3868 3900 4647 4679 6991 Size: 32 11 32 656 32 747 32 2312 Cuts at: 6991 7023 7065 7097 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2312 857 747 656 565 135 79 42 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 5694 5940 669 Size: 988 281 3756 246 3079 Fragments arranged by size: 3756 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4695 5238 748 Size: 3947 543 3860 Fragments arranged by size: 3947 3860 543 HpaI GTT'AAC Cuts at: 5940 5940 Size: 8350 KpnI G_GTAC'C Cuts at: 5675 5675 Size: 8350 MfeI C'AATT_G Cuts at: 5949 5949 Size: 8350 MluI A'CGCG_T Cuts at: 8226 8226 Size: 8350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2385 3314 4027 4352 4886 5477 5645 7464 Size: 1080 929 713 325 534 591 168 1819 Cuts at: 7464 7648 1305 Size: 184 2007 Fragments arranged by size: 2007 1819 1080 929 713 591 534 325 184 168 MscI TGG'CCA Cuts at: 10 64 1489 1832 2631 2924 10 Size: 54 1425 343 799 293 5436 Fragments arranged by size: 5436 1425 799 343 293 54 MslI CAynn'nnrTG Cuts at: 518 1166 3146 3160 3443 4910 5306 5475 6308 Size: 648 1980 14 283 1467 396 169 833 Cuts at: 6308 6667 6826 518 Size: 359 159 2042 Fragments arranged by size: 2042 1980 1467 833 648 396 359 283 169 159 14 MspA1I CmG'CkG Cuts at: 1606 3554 3983 4564 4973 5766 6443 7591 7836 Size: 1948 429 581 409 793 677 1148 245 Cuts at: 7836 1606 Size: 2120 Fragments arranged by size: 2120 1948 1148 793 677 581 429 409 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3811 513 Size: 552 939 1807 5052 Fragments arranged by size: 5052 1807 939 552 NdeI CA'TA_TG Cuts at: 387 2035 5034 387 Size: 1648 2999 3703 Fragments arranged by size: 3703 2999 1648 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8350 NotI GC'GGCC_GC Cuts at: 5797 5797 Size: 8350 NspI r_CATG'y Cuts at: 1132 1229 3151 3434 4259 5315 5708 7253 1132 Size: 97 1922 283 825 1056 393 1545 2229 Fragments arranged by size: 2229 1922 1545 1056 825 393 283 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8350 PciI A'CATG_T Cuts at: 3147 4255 7249 3147 Size: 1108 2994 4248 Fragments arranged by size: 4248 2994 1108 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8350 PfoI T'CCnGG_A Cuts at: 2109 2109 Size: 8350 PmeI GTTT'AAAC Cuts at: 5654 5654 Size: 8350 PmlI CAC'GTG Cuts at: 2314 2314 Size: 8350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6419 6451 7932 7964 6419 Size: 32 1481 32 6805 Fragments arranged by size: 6805 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8350 PsiI TTA'TAA Cuts at: 4176 5460 5920 4176 Size: 1284 460 6606 Fragments arranged by size: 6606 1284 460 PspXI vC'TCGA_Gb Cuts at: 2217 2217 Size: 8350 PstI C_TGCA'G Cuts at: 830 2272 3828 4603 5113 5702 830 Size: 1442 1556 775 510 589 3478 Fragments arranged by size: 3478 1556 1442 775 589 510 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8350 PvuII CAG'CTG Cuts at: 3554 3983 4973 3554 Size: 429 990 6931 Fragments arranged by size: 6931 990 429 SacI G_AGCT'C Cuts at: 3106 3106 Size: 8350 SalI G'TCGA_C Cuts at: 1936 5692 1936 Size: 3756 4594 SapI GCTCTTCn'nnn_ Cuts at: 3348 4818 3348 Size: 1470 6880 SbfI CC_TGCA'GG Cuts at: 5702 5702 Size: 8350 ScaI AGT'ACT Cuts at: 2752 3727 4753 6598 8310 2752 Size: 975 1026 1845 1712 2792 Fragments arranged by size: 2792 1845 1712 1026 975 SfcI C'TryA_G Cuts at: 826 1045 2268 3097 3824 3995 4599 5109 5698 Size: 219 1223 829 727 171 604 510 589 Cuts at: 5698 6833 7514 7705 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1223 1135 829 727 681 604 589 510 219 191 171 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8350 SmaI CCC'GGG Cuts at: 1872 5677 1872 Size: 3805 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2217 2972 3246 5431 6454 7355 Size: 197 926 274 755 274 2185 1023 901 Cuts at: 7355 7617 820 Size: 262 1553 Fragments arranged by size: 2185 1553 1023 926 901 755 274 274 262 197 SnaBI TAC'GTA Cuts at: 493 6141 493 Size: 5648 2702 SpeI A'CTAG_T Cuts at: 152 5252 152 Size: 5100 3250 SphI G_CATG'C Cuts at: 1132 5315 5708 1132 Size: 4183 393 3774 Fragments arranged by size: 4183 3774 393 SspI AAT'ATT Cuts at: 5 52 2986 4717 6274 5 Size: 47 2934 1731 1557 2081 Fragments arranged by size: 2934 2081 1731 1557 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8350 StyI C'CwwG_G Cuts at: 513 1065 2004 3210 3811 5789 513 Size: 552 939 1206 601 1978 3074 Fragments arranged by size: 3074 1978 1206 939 601 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4819 6377 6394 6547 6732 7177 325 Size: 1255 3239 1558 17 153 185 445 1498 Fragments arranged by size: 3239 1558 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2557 2750 3725 Size: 274 80 33 51 1443 580 193 975 Cuts at: 3725 4751 5550 6596 8308 96 Size: 1026 799 1046 1712 138 Fragments arranged by size: 1712 1443 1046 1026 975 799 580 274 193 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2472 2504 3984 4016 8310 8342 2472 Size: 32 1480 32 4294 32 2480 Fragments arranged by size: 4294 2480 1480 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4027 1316 Size: 2711 5639 XbaI T'CTAG_A Cuts at: 3595 4684 5686 3595 Size: 1089 1002 6259 Fragments arranged by size: 6259 1089 1002 XhoI C'TCGA_G Cuts at: 1943 2217 1943 Size: 274 8076 XmaI C'CCGG_G Cuts at: 1870 5675 1870 Size: 3805 4545 XmnI GAAnn'nnTTC Cuts at: 2796 3180 6479 2796 Size: 384 3299 4667 Fragments arranged by size: 4667 3299 384 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6158 276 Size: 53 83 186 936 4624 2468 Fragments arranged by size: 4624 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI