Restriction map for
pFN21AB7665
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AvaII AviII AvrII BalI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HapII HindIII HpaI HpaII HphI
KasI Ksp632I MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tru9I Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 404 GGTACC G^GTACC
AcsI 111 GAATTC [GA]^AATT[CT]
AcyI 176 GGCGTC G[GA]^CG[CT]C
ApoI 111 GAATTC [GA]^AATT[CT]
Asp718I 404 GGTACC G^GTACC
AspHI 411 GAGCTC G[AT]GC[AT]^C
AvaI 78 CTCGGG C^[CT]CG[GA]G
BamHI 438 GGATCC G^GATCC
BanI 404 GGTACC G^G[CT][GA]CC
BanII 411 GAGCTC G[GA]GC[CT]^C
BbsI 215 GAAGAC GAAGAC(2/6)
BbvCI 943 CCTCAGC CCTCAGC(-5/-2)
BfaI 92 CTAG C^TAG
BglI 811 GCCACCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 411 GAGCTC G[AGT]GC[ACT]^C
BpmI 589 CTCCAG CTGGAG(16/14)
BpuAI 215 GAAGAC GAAGAC(2/6)
BsaHI 176 GGCGTC G[GA]^CG[CT]C
BsaI 46 GAGACC GGTCTC(1/5)
BsgI 821 CTGCAC GTGCAG(16/14)
BsiHKAI 411 GAGCTC G[AT]GC[AT]^C
BsmBI 19 GAGACG CGTCTC(1/5)
BsoBI 78 CTCGGG C^[CT]CG[GA]G
Bsp1286I 411 GAGCTC G[AGT]GC[ACT]^C
BspHI 362 TCATGA T^CATGA
BspMI 572 ACCTGC ACCTGC(4/8)
BsrBI 535 CCGCTC CCGCTC(-3/-3)
BstXI 50 CCATCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI 111 GAATTC G^AATTC
HaeII 5 AGCGCC [GA]GCGC^[CT]
Hin1I 176 GGCGTC G[GA]^CG[CT]C
HincII 618 GTCAAC GT[CT]^[GA]AC
HindII 618 GTCAAC GT[CT]^[GA]AC
KpnI 404 GGTACC GGTAC^C
MaeI 92 CTAG C^TAG
MslI 51 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
RcaI 362 TCATGA T^CATGA
SacI 411 GAGCTC GAGCT^C
ScaI 88 AGTACT AGT^ACT
SspI 273 AATATT AAT^ATT
StuI 423 AGGCCT AGG^CCT
TaqI 53 TCGA T^CGA
TthHB8I 53 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 89 GTAC GT^AC
405 GTAC
BsmFI 991 GGGAC GGGAC(10/14)
774 GTCCC
BsmI 716 GAATGC GAATGC(1/-1)
853 GCATTC
BsrI 551 ACTGG ACTGG(1/-1)
938 CCAGT
BssKI 426 CCTGG ^CC[ACGT]GG
986 CCTGG
BstNI 426 CCTGG CC^[AT]GG
986 CCTGG
BstYI 247 GGATCT [GA]^GATC[CT]
438 GGATCC
Cfr13I 519 GGCCC G^G[ACGT]CC
554 GGGCC
EcoRII 426 CCTGG ^CC[AT]GG
986 CCTGG
EcoT14I 353 CCATGG C^C[AT][AT]GG
515 CCATGG
KspI 815 CCGCGG CCGC^GG
893 CCGCGG
MboII 215 GAAGA GAAGA(8/7)
300 GAAGA
MflI 247 GGATCT [GA]^GATC[CT]
438 GGATCC
MvaI 426 CCTGG CC^[AT]GG
986 CCTGG
NcoI 353 CCATGG C^CATGG
515 CCATGG
NlaIV 404 GGTACC GG[ACGT]^[ACGT]CC
438 GGATCC
PleI 430 GACTC GAGTC(4/5)
616 GAGTC
RsaI 89 GTAC GT^AC
405 GTAC
SacII 815 CCGCGG CCGC^GG
893 CCGCGG
Sau96I 519 GGCCC G^G[ACGT]CC
554 GGGCC
ScrFI 426 CCTGG CC^[ACGT]GG
986 CCTGG
SfaNI 303 GATGC GCATC(5/9)
546 GCATC
StyI 353 CCATGG C^C[AT][AT]GG
515 CCATGG
TfiI 374 GATTC G^A[AT]TC
703 GATTC
Tsp509I 73 AATT ^AATT
112 AATT
XhoII 247 GGATCT [GA]^GATC[CT]
438 GGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BsaJI BseRI BsiYI BslI BsmAI
BstF5I BstUI Cac8I CfoI DdeI
DpnII DsaI EaeI FauI Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HinfI ItaI MaeII MboI MnlI
MspA1I MvnI MwoI NdeII NlaIII
Sau3AI TseI TspRI