(Circular) (Six-base) MAPSORT of: pFN21AB7665.seq Check: 2971 from: 1 to: 5716 pFN21AB7665 5716 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2586 2586 Size: 5716 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3059 1937 Size: 1122 4594 Acc65I G'GTAC_C Cuts at: 2409 3037 2409 Size: 628 5088 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2297 2634 3779 5163 5431 1632 Size: 665 337 1145 1384 268 1917 Fragments arranged by size: 1917 1384 1145 665 337 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 4615 5592 1203 Size: 3412 977 1327 Fragments arranged by size: 3412 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 3459 5031 3459 Size: 1572 4144 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 2116 3025 3251 3403 5512 1493 Size: 623 909 226 152 2109 1697 Fragments arranged by size: 2109 1697 909 623 226 152 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 2083 3041 1870 Size: 73 140 958 4545 Fragments arranged by size: 4545 958 140 73 BamHI G'GATC_C Cuts at: 2443 3046 3413 2443 Size: 603 367 4746 Fragments arranged by size: 4746 603 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2409 3037 4492 618 Size: 325 217 627 622 628 1455 1842 Fragments arranged by size: 1842 1455 628 627 622 325 217 BanII G_rGCy'C Cuts at: 1391 2420 3035 1391 Size: 1029 615 4072 Fragments arranged by size: 4072 1029 615 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2227 928 Size: 971 328 4417 Fragments arranged by size: 4417 971 328 BbvCI CC'TCA_GC Cuts at: 2949 2949 Size: 5716 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2822 4327 136 Size: 107 122 71 631 605 1150 1505 1525 Fragments arranged by size: 1525 1505 1150 631 605 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 3133 3133 Size: 5716 Bme1580I G_kGCm'C Cuts at: 1790 3777 4933 1790 Size: 1987 1156 2573 Fragments arranged by size: 2573 1987 1156 BmgBI CAC'GTC Cuts at: 1318 2062 2940 1318 Size: 744 878 4094 Fragments arranged by size: 4094 878 744 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2566 3447 4405 Size: 416 53 83 399 24 1149 881 958 Cuts at: 4405 442 Size: 1753 Fragments arranged by size: 1753 1149 958 881 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2047 2047 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 4376 1239 Size: 1340 1797 2579 Fragments arranged by size: 2579 1797 1340 Bpu10I CC'TnA_GC Cuts at: 2414 2949 2414 Size: 535 5181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3841 4706 5004 3841 Size: 865 298 4553 Fragments arranged by size: 4553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2045 4379 882 Size: 617 546 2334 2219 Fragments arranged by size: 2334 2219 617 546 BsaAI yAC'GTr Cuts at: 493 1204 3507 493 Size: 711 2303 2702 Fragments arranged by size: 2702 2303 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2182 3523 3905 4540 Size: 53 83 186 936 649 1341 382 635 Cuts at: 4540 275 Size: 1451 Fragments arranged by size: 1451 1341 936 649 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2402 2432 3000 3030 1178 Size: 30 165 30 999 30 568 30 3864 Fragments arranged by size: 3864 999 568 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2428 2672 1186 Size: 850 392 244 4230 Fragments arranged by size: 4230 850 392 244 BseYI C'CCAG_C Cuts at: 2490 4919 5415 2490 Size: 2429 496 2791 Fragments arranged by size: 2791 2429 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2811 1163 Size: 573 1075 4068 Fragments arranged by size: 4068 1075 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3166 3927 4955 1540 Size: 275 186 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2420 3777 3862 4933 2420 Size: 1357 85 1071 3203 Fragments arranged by size: 3203 1357 1071 85 BsmI GAATG_Cn' Cuts at: 2727 2858 3227 3320 2727 Size: 131 369 93 5123 Fragments arranged by size: 5123 369 131 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2018 2018 Size: 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2420 3035 3777 3862 4933 1391 Size: 399 630 615 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 630 615 399 85 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 2367 3605 5335 2367 Size: 1238 1730 2748 Fragments arranged by size: 2748 1730 1238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2586 3071 844 Size: 1742 485 3489 Fragments arranged by size: 3489 1742 485 BsrBI CCG'CTC Cuts at: 1394 1823 2542 3603 1394 Size: 429 719 1061 3507 Fragments arranged by size: 3507 1061 719 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4211 4385 65 Size: 1183 2963 174 1396 Fragments arranged by size: 2963 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4360 4547 1700 Size: 110 2550 187 2869 Fragments arranged by size: 2869 2550 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 3776 4788 3776 Size: 1012 4704 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2062 Size: 5716 BstYI r'GATC_y Cuts at: 1972 2252 2443 3046 3413 3798 3815 5267 5353 Size: 280 191 603 367 385 17 1452 86 Cuts at: 5353 5365 5711 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 603 385 367 346 280 191 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2358 2421 2520 2668 2730 2820 2898 Size: 552 1293 63 99 148 62 90 78 Cuts at: 2898 513 Size: 3331 Fragments arranged by size: 3331 1293 552 148 99 90 78 63 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 2898 2968 3232 4026 4046 2898 Size: 70 264 794 20 4568 Fragments arranged by size: 4568 794 264 70 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 3020 3020 Size: 5716 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2505 2688 2895 Size: 54 1425 50 275 18 675 183 207 Cuts at: 2895 2901 3163 4052 5681 8 Size: 6 262 889 1629 43 Fragments arranged by size: 1629 1425 889 675 275 262 207 183 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3646 1353 Size: 2293 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4303 4677 4823 387 Size: 1064 138 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2418 2418 Size: 5716 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2579 2634 3779 4376 5163 5431 Size: 393 665 282 55 1145 597 787 268 Cuts at: 5431 1239 Size: 1524 Fragments arranged by size: 1524 1145 787 665 597 393 282 268 55 EcoRI G'AATT_C Cuts at: 2116 3025 3403 2116 Size: 909 378 4429 Fragments arranged by size: 4429 909 378 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 2014 4863 1678 Size: 336 2849 2531 Fragments arranged by size: 2849 2531 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2281 2313 4357 4389 4431 Size: 32 135 32 709 32 2044 32 42 Cuts at: 4431 4463 1373 Size: 32 2626 Fragments arranged by size: 2626 2044 709 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2625 3060 3306 669 Size: 988 281 687 435 246 3079 Fragments arranged by size: 3079 988 687 435 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KpnI G_GTAC'C Cuts at: 2413 3041 2413 Size: 628 5088 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2147 2986 4830 5014 1305 Size: 842 839 1844 184 2007 Fragments arranged by size: 2007 1844 842 839 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 3674 4033 4192 518 Size: 648 894 1614 359 159 2042 Fragments arranged by size: 2042 1614 894 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2449 2494 2822 2900 3132 3809 4957 5202 Size: 843 45 328 78 232 677 1148 245 Cuts at: 5202 1606 Size: 2120 Fragments arranged by size: 2120 1148 843 677 328 245 232 78 45 NcoI C'CATG_G Cuts at: 513 1065 2358 2520 513 Size: 552 1293 162 3709 Fragments arranged by size: 3709 1293 552 162 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 3074 4619 1132 Size: 97 1845 1545 2229 Fragments arranged by size: 2229 1845 1545 97 PasI CC'CwG_GG Cuts at: 1348 2991 1348 Size: 1643 4073 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5716 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2066 2098 3785 3817 5298 5330 2066 Size: 32 1687 32 1481 32 2452 Fragments arranged by size: 2452 1687 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PstI C_TGCA'G Cuts at: 830 3068 830 Size: 2238 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 SacI G_AGCT'C Cuts at: 2420 2420 Size: 5716 SacII CC_GC'GG Cuts at: 2823 2901 2823 Size: 78 5638 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 2095 3964 5676 2095 Size: 1869 1712 2135 Fragments arranged by size: 2135 1869 1712 SfcI C'TryA_G Cuts at: 826 1045 3064 4199 4880 5071 826 Size: 219 2019 1135 681 191 1471 Fragments arranged by size: 2019 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3820 4721 4983 820 Size: 197 926 1877 901 262 1553 Fragments arranged by size: 1877 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 2280 3640 5 Size: 47 2228 1360 2081 Fragments arranged by size: 2228 2081 1360 47 StuI AGG'CCT Cuts at: 1517 2430 1517 Size: 913 4803 StyI C'CwwG_G Cuts at: 513 1065 2358 2520 3155 513 Size: 552 1293 162 635 3074 Fragments arranged by size: 3074 1293 635 552 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2504 3743 3760 3913 4098 4543 325 Size: 1255 924 1239 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1239 924 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2093 3962 5674 Size: 274 80 33 51 1443 116 1869 1712 Cuts at: 5674 96 Size: 138 Fragments arranged by size: 1869 1712 1443 274 138 116 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2820 2852 2929 2961 5676 5708 2820 Size: 32 77 32 2715 32 2828 Fragments arranged by size: 2828 2715 77 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 3845 3845 Size: 5716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI