Restriction map for
pFN21AB7665
(Linear) (Six-base) MAPSORT of: KIBB7665.seq Check: 2262 from: 1 to: 1011
KIBB7665.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 581 1011
Size: 581 430
Acc65I G'GTAC_C
Cuts at: 0 404 1011
Size: 404 607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 629 1011
Size: 292 337 382
Fragments arranged by size:
382 337 292
ApoI r'AATT_y
Cuts at: 0 111 1011
Size: 111 900
AvaI C'yCGr_G
Cuts at: 0 78 1011
Size: 78 933
BamHI G'GATC_C
Cuts at: 0 438 1011
Size: 438 573
BanI G'GyrC_C
Cuts at: 0 404 1011
Size: 404 607
BanII G_rGCy'C
Cuts at: 0 415 1011
Size: 415 596
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 1011
Size: 222 789
BbvCI CC'TCA_GC
Cuts at: 0 944 1011
Size: 944 67
BglI GCCn_nnn'nGGC
Cuts at: 0 817 1011
Size: 817 194
BmgBI CAC'GTC
Cuts at: 0 57 935 1011
Size: 57 878 76
Fragments arranged by size:
878 76 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 561 1011
Size: 561 450
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 1011
Size: 42 969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 1011
Size: 574 437
Bpu10I CC'TnA_GC
Cuts at: 0 409 944 1011
Size: 409 535 67
Fragments arranged by size:
535 409 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 40 1011
Size: 40 971
BsaHI Gr'CG_yC
Cuts at: 0 177 1011
Size: 177 834
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 397 427 1011
Size: 397 30 584
Fragments arranged by size:
584 397 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 423 667 1011
Size: 31 392 244 344
Fragments arranged by size:
392 344 244 31
BseYI C'CCAG_C
Cuts at: 0 485 1011
Size: 485 526
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1011
Size: 806 205
BsiHKAI G_wGCw'C
Cuts at: 0 415 1011
Size: 415 596
BsmI GAATG_Cn'
Cuts at: 0 722 853 1011
Size: 722 131 158
Fragments arranged by size:
722 158 131
BsmBI CGTCTCn'nnnn_
Cuts at: 0 13 1011
Size: 13 998
Bsp1286I G_dGCh'C
Cuts at: 0 415 1011
Size: 415 596
BspHI T'CATG_A
Cuts at: 0 362 1011
Size: 362 649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 581 1011
Size: 581 430
BsrBI CCG'CTC
Cuts at: 0 537 1011
Size: 537 474
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 1011
Size: 57 954
BstYI r'GATC_y
Cuts at: 0 247 438 1011
Size: 247 191 573
Fragments arranged by size:
573 247 191
BtgI C'CryG_G
Cuts at: 0 353 416 515 663 725 815 893 1011
Size: 353 63 99 148 62 90 78 118
Fragments arranged by size:
353 148 118 99 90 78 63 62
BtsI GCAGTG_nn'
Cuts at: 0 893 963 1011
Size: 893 70 48
Fragments arranged by size:
893 70 48
EaeI y'GGCC_r
Cuts at: 0 500 683 890 896 1011
Size: 500 183 207 6 115
Fragments arranged by size:
500 207 183 115 6
EcoICRI GAG'CTC
Cuts at: 0 413 1011
Size: 413 598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 574 629 1011
Size: 292 282 55 382
Fragments arranged by size:
382 292 282 55
EcoRI G'AATT_C
Cuts at: 0 111 1011
Size: 111 900
HaeII r_GCGC'y
Cuts at: 0 9 1011
Size: 9 1002
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 1011
Size: 276 32 703
Fragments arranged by size:
703 276 32
HincII GTy'rAC
Cuts at: 0 620 1011
Size: 620 391
KpnI G_GTAC'C
Cuts at: 0 408 1011
Size: 408 603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 981 1011
Size: 142 839 30
Fragments arranged by size:
839 142 30
MslI CAynn'nnrTG
Cuts at: 0 55 1011
Size: 55 956
MspA1I CmG'CkG
Cuts at: 0 444 489 817 895 1011
Size: 444 45 328 78 116
Fragments arranged by size:
444 328 116 78 45
NcoI C'CATG_G
Cuts at: 0 353 515 1011
Size: 353 162 496
Fragments arranged by size:
496 353 162
PasI CC'CwG_GG
Cuts at: 0 986 1011
Size: 986 25
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 1011
Size: 61 32 918
Fragments arranged by size:
918 61 32
SacI G_AGCT'C
Cuts at: 0 415 1011
Size: 415 596
SacII CC_GC'GG
Cuts at: 0 818 896 1011
Size: 818 78 115
Fragments arranged by size:
818 115 78
ScaI AGT'ACT
Cuts at: 0 90 1011
Size: 90 921
SspI AAT'ATT
Cuts at: 0 275 1011
Size: 275 736
StuI AGG'CCT
Cuts at: 0 425 1011
Size: 425 586
StyI C'CwwG_G
Cuts at: 0 353 515 1011
Size: 353 162 496
Fragments arranged by size:
496 353 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 1011
Size: 499 512
TatI w'GTAC_w
Cuts at: 0 88 1011
Size: 88 923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 815 847 924 956 1011
Size: 815 32 77 32 55
Fragments arranged by size:
815 77 55 32 32
Enzymes that do cut:
AarI Acc65I AcuI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BglI BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BstXI BstYI BtgI
BtsI EaeI EcoICRI Eco57MI EcoRI HaeII Hin4I HincII
KpnI MmeI MslI MspA1I NcoI PasI PpiI SacI
SacII ScaI SspI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmtI BpuEI BsaAI BsaBI BsaWI
BsiEI BsiWI BspEI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI