Restriction map for
pFN21AA0698
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Aor51HI AscI
AspEI AvaI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BglII
BlnI BsaAI BsaHI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CfoI ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 2403 CTTAAG C^TTAAG
Alw44I 53 GTGCAC G^TGCAC
AocI 2121 CCTTAGG CC^T[ACGT]AGG
ApaLI 53 GTGCAC G^TGCAC
AseI 1066 ATTAAT AT^TAAT
AsnI 1066 ATTAAT AT^TAAT
AspI 2347 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 610 GTCTTC GAAGAC(2/6)
BbvCI 583 GCTGAGG CCTCAGC(-5/-2)
BfaI 1723 CTAG C^TAG
BfrI 2403 CTTAAG C^TTAAG
BlpI 1753 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1753 GCTGAGC GC^T[ACGT]AGC
BpuAI 610 GTCTTC GAAGAC(2/6)
BsaI 2368 GAGACC GGTCTC(1/5)
BsaWI 1445 ACCGGA [AT]^CCGG[AT]
Bsp1407I 689 TGTACA T^GTACA
BsrFI 772 GCCGGT [GA]^CCGG[CT]
BsrGI 689 TGTACA T^GTACA
Bsu36I 2121 CCTTAGG CC^T[ACGT]AGG
CelII 1753 GCTGAGC GC^T[ACGT]AGC
Cfr10I 772 GCCGGT [GA]^CCGG[CT]
Eco81I 2121 CCTTAGG CC^T[ACGT]AGG
EcoRI 928 GAATTC G^AATTC
EcoRV 1657 GATATC GAT^ATC
FauI 864 CCCGC CCCGC(4/6)
HgaI 2618 GACGC GACGC(5/10)
HindIII 2653 AAGCTT A^AGCTT
MaeI 1723 CTAG C^TAG
PflFI 2347 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2029 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1066 ATTAAT AT^TAAT
PvuII 992 CAGCTG CAG^CTG
SacI 456 GAGCTC GAGCT^C
SapI 453 GAAGAGC GCTCTTC(1/4)
SfiI 853 GGCCTGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 2403 CTTAAG C^T[CT][GA]AG
SspBI 689 TGTACA T^GTACA
Tth111I 2347 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2349 CCATGTCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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Acc65I 220 GGTACC G^GTACC
2591 GGTACC
AlwNI 130 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1151 CAGAGACTG
Asp700I 499 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
928 GAATTCTTTC
Asp718I 220 GGTACC G^GTACC
2591 GGTACC
AspHI 53 GTGCAC G[AT]GC[AT]^C
456 GAGCTC
BcnI 579 CCGGG CC^[GC]GG
2250 CCGGG
BpmI 459 CTCCAG CTGGAG(16/14)
1921 CTGGAG
BsaBI 547 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2185 GATGTGGATC
BsiHKAI 53 GTGCAC G[AT]GC[AT]^C
456 GAGCTC
BstXI 599 CCATTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1347 CCAGTGTCCTGG
KpnI 220 GGTACC GGTAC^C
2591 GGTACC
MaeII 63 ACGT A^CGT
1576 ACGT
MamI 547 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2185 GATGTGGATC
NciI 579 CCGGG CC^[GC]GG
2250 CCGGG
PflMI 1594 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2181 CCAAGATGTGG
PstI 824 CTGCAG CTGCA^G
1850 CTGCAG
ScaI 341 AGTACT AGT^ACT
400 AGTACT
SexAI 216 ACCTGGT A^CC[AT]GGT
223 ACCTGGT
Van91I 1594 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2181 CCAAGATGTGG
XmnI 499 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
928 GAATTCTTTC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
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AciI AcsI AfaI AflIII AluI
AlwI ApaI ApoI AvaII BalI
BamHI BanI BanII BbvI BglI
BmyI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII