(Circular) (Six-base) MAPSORT of: pFN21AA0698.seq Check: 4271 from: 1 to: 7378 pFN21AA0698 7378 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3147 3147 Size: 7378 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5188 278 Size: 53 83 186 936 3652 2468 Fragments arranged by size: 3652 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4721 1937 Size: 2784 4594 Acc65I G'GTAC_C Cuts at: 2225 4596 4699 2225 Size: 2371 103 4904 Fragments arranged by size: 4904 2371 103 AclI AA'CG_TT Cuts at: 1563 5505 5878 1563 Size: 3942 373 3063 Fragments arranged by size: 3942 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3188 5441 6825 7093 1632 Size: 1556 2253 1384 268 1917 Fragments arranged by size: 2253 1917 1556 1384 268 AflII C'TTAA_G Cuts at: 820 1017 4408 820 Size: 197 3391 3790 Fragments arranged by size: 3790 3391 197 AflIII A'CryG_T Cuts at: 1203 2052 2068 3108 3876 6277 7254 1203 Size: 849 16 1040 768 2401 977 1327 Fragments arranged by size: 2401 1327 1040 977 849 768 16 AgeI A'CCGG_T Cuts at: 6209 6209 Size: 7378 AhdI GACnn_n'nnGTC Cuts at: 6107 6107 Size: 7378 AleI CACnn'nnGTG Cuts at: 1166 2165 2192 1166 Size: 999 27 6352 Fragments arranged by size: 6352 999 27 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3212 4109 1184 Size: 2028 897 4453 Fragments arranged by size: 4453 2028 897 AlwNI CAG_nnn'CTG Cuts at: 2140 3161 5121 6693 2140 Size: 1021 1960 1572 2825 Fragments arranged by size: 2825 1960 1572 1021 ApaI G_GGCC'C Cuts at: 2870 3819 3900 2870 Size: 949 81 6348 Fragments arranged by size: 6348 949 81 ApaLI G'TGCA_C Cuts at: 2058 5435 6591 2058 Size: 3377 1156 2845 Fragments arranged by size: 3377 2845 1156 ApoI r'AATT_y Cuts at: 1493 2035 2416 2851 2933 3962 4687 4913 5065 Size: 542 381 435 82 1029 725 226 152 Cuts at: 5065 7174 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1029 725 542 435 381 226 152 82 AseI AT'TA_AT Cuts at: 160 1032 3072 5932 160 Size: 872 2040 2860 1606 Fragments arranged by size: 2860 2040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7378 AvaI C'yCGr_G Cuts at: 1870 1943 4703 1870 Size: 73 2760 4545 Fragments arranged by size: 4545 2760 73 BamHI G'GATC_C Cuts at: 2794 3588 3862 4042 4708 5075 2794 Size: 794 274 180 666 367 5097 Fragments arranged by size: 5097 794 666 367 274 180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2209 2225 2891 3135 4596 Size: 325 217 627 422 16 666 244 1461 Cuts at: 4596 4699 6154 618 Size: 103 1455 1842 Fragments arranged by size: 1842 1461 1455 666 627 422 325 244 217 103 16 BanII G_rGCy'C Cuts at: 1391 2465 2870 3531 3819 3900 3933 4697 1391 Size: 1074 405 661 288 81 33 764 4072 Fragments arranged by size: 4072 1074 764 661 405 288 81 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2608 928 Size: 971 709 5698 Fragments arranged by size: 5698 971 709 BbvCI CC'TCA_GC Cuts at: 2589 2589 Size: 7378 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5569 5603 1903 Size: 34 50 34 3548 34 3678 Fragments arranged by size: 3678 3548 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5270 6480 5270 Size: 1210 6168 BclI T'GATC_A Cuts at: 1548 1548 Size: 7378 BfrBI ATG'CAT Cuts at: 2008 2539 3064 4096 2008 Size: 531 525 1032 5290 Fragments arranged by size: 5290 1032 531 525 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2865 3306 3918 Size: 107 122 71 631 605 1193 441 612 Cuts at: 3918 5989 136 Size: 2071 1525 Fragments arranged by size: 2071 1525 1193 631 612 605 441 122 107 71 BglII A'GATC_T Cuts at: 7373 7373 Size: 7378 BlpI GC'TnA_GC Cuts at: 3759 4795 3759 Size: 1036 6342 Bme1580I G_kGCm'C Cuts at: 1790 2062 2214 2483 2870 3155 3819 3900 4455 Size: 272 152 269 387 285 664 81 555 Cuts at: 4455 5439 6595 1790 Size: 984 1156 2573 Fragments arranged by size: 2573 1156 984 664 555 387 285 272 269 152 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7378 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2418 3346 5109 Size: 416 53 83 399 24 1001 928 1763 Cuts at: 5109 6067 442 Size: 958 1753 Fragments arranged by size: 1763 1753 1001 958 928 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7378 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2449 3947 6038 1239 Size: 1210 1498 2091 2579 Fragments arranged by size: 2579 2091 1498 1210 Bpu10I CC'TnA_GC Cuts at: 2043 2589 2790 3512 2043 Size: 546 201 722 5909 Fragments arranged by size: 5909 722 546 201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5503 6368 6666 5503 Size: 865 298 6215 Fragments arranged by size: 6215 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 6041 882 Size: 617 2868 1674 2219 Fragments arranged by size: 2868 2219 1674 617 BsaAI yAC'GTr Cuts at: 493 1204 5169 493 Size: 711 3965 2702 Fragments arranged by size: 3965 2702 711 BsaBI GATnn'nnATC Cuts at: 2556 4194 7372 2556 Size: 1638 3178 2562 Fragments arranged by size: 3178 2562 1638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5185 5567 6202 275 Size: 53 83 186 936 3652 382 635 1451 Fragments arranged by size: 3652 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3450 5811 6209 6483 6630 7041 3450 Size: 2361 398 274 147 411 3787 Fragments arranged by size: 3787 2361 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2615 2645 4143 4173 1178 Size: 30 165 30 1212 30 1498 30 4383 Fragments arranged by size: 4383 1498 1212 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7378 BseYI C'CCAG_C Cuts at: 2634 3456 4489 6581 7077 2634 Size: 822 1033 2092 496 2935 Fragments arranged by size: 2935 2092 1033 822 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2902 2922 3218 3243 1163 Size: 573 1166 20 296 25 5298 Fragments arranged by size: 5298 1166 573 296 25 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4828 5589 6617 1540 Size: 275 186 2827 761 1028 2301 Fragments arranged by size: 2827 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2062 2465 5439 5524 6595 2062 Size: 403 2974 85 1071 2845 Fragments arranged by size: 2974 2845 1071 403 85 BsmI GAATG_Cn' Cuts at: 4889 4982 4889 Size: 93 7285 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2214 2465 2483 2870 3155 3531 Size: 399 272 152 251 18 387 285 376 Cuts at: 3531 3819 3900 3933 4455 4697 5439 5524 6595 Size: 288 81 33 522 242 742 85 1071 Cuts at: 6595 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 522 399 387 376 288 285 272 251 242 152 85 81 33 18 BspEI T'CCGG_A Cuts at: 7041 7041 Size: 7378 BspHI T'CATG_A Cuts at: 5267 6997 5267 Size: 1730 5648 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2331 3102 3147 4733 844 Size: 1487 771 45 1586 3489 Fragments arranged by size: 3489 1586 1487 771 45 BsrBI CCG'CTC Cuts at: 1394 1823 5265 1394 Size: 429 3442 3507 Fragments arranged by size: 3507 3442 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2104 2989 4483 5873 6047 65 Size: 1183 856 885 1494 1390 174 1396 Fragments arranged by size: 1494 1396 1390 1183 885 856 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2777 6022 6209 1700 Size: 110 967 3245 187 2869 Fragments arranged by size: 3245 2869 967 187 110 BsrGI T'GTAC_A Cuts at: 96 2694 96 Size: 2598 4780 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7378 BssSI C'ACGA_G Cuts at: 5438 6450 5438 Size: 1012 6366 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7378 BstBI TT'CG_AA Cuts at: 6182 6182 Size: 7378 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7378 BstXI CCAn_nnnn'nTGG Cuts at: 2611 3359 2611 Size: 748 6630 BstYI r'GATC_y Cuts at: 1972 2794 2833 3588 3843 3862 4042 4195 4270 Size: 822 39 755 255 19 180 153 75 Cuts at: 4270 4500 4708 5075 5460 5477 6929 7015 7027 Size: 230 208 367 385 17 1452 86 12 Cuts at: 7027 7373 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 822 755 385 367 346 255 230 208 180 153 86 75 39 19 17 12 Bsu36I CC'TnA_GG Cuts at: 4127 4127 Size: 7378 BtgI C'CryG_G Cuts at: 513 1065 2128 2149 2304 2656 3934 4030 4179 Size: 552 1063 21 155 352 1278 96 149 Cuts at: 4179 513 Size: 3712 Fragments arranged by size: 3712 1278 1063 552 352 155 149 96 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6639 Fragments arranged by size: 6639 683 56 BtsI GCAGTG_nn' Cuts at: 2784 3864 4336 4455 4894 5688 5708 2784 Size: 1080 472 119 439 794 20 4454 Fragments arranged by size: 4454 1080 794 472 439 119 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7378 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7343 DraI TTT'AAA Cuts at: 4682 4682 Size: 7378 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6385 809 Size: 507 5069 1802 Fragments arranged by size: 5069 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3255 3361 3596 Size: 54 1425 50 275 18 1425 106 235 Cuts at: 3596 3746 4176 4308 4825 5714 7343 8 Size: 150 430 132 517 889 1629 43 Fragments arranged by size: 1629 1425 1425 889 517 430 275 235 150 132 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4825 1537 Size: 275 3013 4090 Fragments arranged by size: 4090 3013 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2453 2951 4385 5308 1353 Size: 1100 498 1434 923 3423 Fragments arranged by size: 3423 1434 1100 923 498 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5965 6339 6485 387 Size: 1064 138 4376 374 146 1280 Fragments arranged by size: 4376 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2463 2463 Size: 7378 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2449 3188 3947 5441 6038 6825 7093 Size: 393 817 739 759 1494 597 787 268 Cuts at: 7093 1239 Size: 1524 Fragments arranged by size: 1524 1494 817 787 759 739 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3896 3897 4035 3896 Size: 1 138 7239 Fragments arranged by size: 7239 138 1 EcoRI G'AATT_C Cuts at: 2933 4687 5065 2933 Size: 1754 378 5246 Fragments arranged by size: 5246 1754 378 EcoRV GAT'ATC Cuts at: 3664 3664 Size: 7378 FspI TGC'GCA Cuts at: 5884 5884 Size: 7378 HaeII r_GCGC'y Cuts at: 1678 6525 1678 Size: 4847 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2693 2725 6019 6051 6093 Size: 32 135 32 1121 32 3294 32 42 Cuts at: 6093 6125 1373 Size: 32 2626 Fragments arranged by size: 3294 2626 1121 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4722 4968 669 Size: 988 281 2784 246 3079 Fragments arranged by size: 3079 2784 988 281 246 HindIII A'AGCT_T Cuts at: 748 4658 748 Size: 3910 3468 HpaI GTT'AAC Cuts at: 4968 4968 Size: 7378 KpnI G_GTAC'C Cuts at: 2229 4600 4703 2229 Size: 2371 103 4904 Fragments arranged by size: 4904 2371 103 MfeI C'AATT_G Cuts at: 4977 4977 Size: 7378 MluI A'CGCG_T Cuts at: 7254 7254 Size: 7378 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2937 2988 3040 6492 6676 1305 Size: 1632 51 52 3452 184 2007 Fragments arranged by size: 3452 2007 1632 184 52 51 MscI TGG'CCA Cuts at: 10 64 1489 1832 3257 3363 3748 4178 4310 Size: 54 1425 343 1425 106 385 430 132 Cuts at: 4310 10 Size: 3078 Fragments arranged by size: 3078 1425 1425 430 385 343 132 106 54 MslI CAynn'nnrTG Cuts at: 518 1166 2163 2165 2192 2609 2813 3717 4215 Size: 648 997 2 27 417 204 904 498 Cuts at: 4215 4341 4359 5336 5695 5854 518 Size: 126 18 977 359 159 2042 Fragments arranged by size: 2042 997 977 904 648 498 417 359 204 159 126 27 18 2 MspA1I CmG'CkG Cuts at: 1606 2757 2872 2999 4794 5471 6619 6864 1606 Size: 1151 115 127 1795 677 1148 245 2120 Fragments arranged by size: 2120 1795 1151 1148 677 245 127 115 NcoI C'CATG_G Cuts at: 513 1065 2128 2304 2656 3934 4030 4179 513 Size: 552 1063 176 352 1278 96 149 3712 Fragments arranged by size: 3712 1278 1063 552 352 176 149 96 NdeI CA'TA_TG Cuts at: 387 387 Size: 7378 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7378 NotI GC'GGCC_GC Cuts at: 4825 4825 Size: 7378 NsiI A_TGCA'T Cuts at: 2010 2541 3066 4098 2010 Size: 531 525 1032 5290 Fragments arranged by size: 5290 1032 531 525 NspI r_CATG'y Cuts at: 1132 1229 2012 2056 2275 2650 3068 3112 3716 Size: 97 783 44 219 375 418 44 604 Cuts at: 3716 3880 4100 4174 4498 4736 6281 1132 Size: 164 220 74 324 238 1545 2229 Fragments arranged by size: 2229 1545 783 604 418 375 324 238 220 219 164 97 74 44 44 PasI CC'CwG_GG Cuts at: 1348 2214 3812 4280 4319 1348 Size: 866 1598 468 39 4407 Fragments arranged by size: 4407 1598 866 468 39 PciI A'CATG_T Cuts at: 2052 3108 3876 6277 2052 Size: 1056 768 2401 3153 Fragments arranged by size: 3153 2401 1056 768 PflMI CCAn_nnn'nTGG Cuts at: 1108 3605 4192 1108 Size: 2497 587 4294 Fragments arranged by size: 4294 2497 587 PfoI T'CCnGG_A Cuts at: 3839 3924 4641 3839 Size: 85 717 6576 Fragments arranged by size: 6576 717 85 PmeI GTTT'AAAC Cuts at: 4682 4682 Size: 7378 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5447 5479 6960 6992 5447 Size: 32 1481 32 5833 Fragments arranged by size: 5833 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4035 4035 Size: 7378 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7378 PsiI TTA'TAA Cuts at: 4948 4948 Size: 7378 PspOMI G'GGCC_C Cuts at: 2866 3815 3896 2866 Size: 949 81 6348 Fragments arranged by size: 6348 949 81 PstI C_TGCA'G Cuts at: 830 2833 3859 4730 830 Size: 2003 1026 871 3478 Fragments arranged by size: 3478 2003 1026 871 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7378 PvuII CAG'CTG Cuts at: 2999 2999 Size: 7378 SacI G_AGCT'C Cuts at: 2465 2465 Size: 7378 SalI G'TCGA_C Cuts at: 1936 4720 1936 Size: 2784 4594 SanDI GG'GwC_CC Cuts at: 4035 4035 Size: 7378 SapI GCTCTTCn'nnn_ Cuts at: 2453 2453 Size: 7378 SbfI CC_TGCA'GG Cuts at: 4730 4730 Size: 7378 ScaI AGT'ACT Cuts at: 2348 2407 5626 7338 2348 Size: 59 3219 1712 2388 Fragments arranged by size: 3219 2388 1712 59 SexAI A'CCwGG_T Cuts at: 2221 2228 2221 Size: 7 7371 SfcI C'TryA_G Cuts at: 826 1045 2829 3855 4061 4726 5861 6542 6733 Size: 219 1784 1026 206 665 1135 681 191 Cuts at: 6733 826 Size: 1471 Fragments arranged by size: 1784 1471 1135 1026 681 665 219 206 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2865 2865 Size: 7378 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7378 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7378 SmaI CCC'GGG Cuts at: 1872 4705 1872 Size: 2833 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4408 5482 6383 6645 820 Size: 197 926 2465 1074 901 262 1553 Fragments arranged by size: 2465 1553 1074 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5169 493 Size: 4676 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7378 SphI G_CATG'C Cuts at: 1132 2012 2275 2650 3068 3716 4100 4498 4736 Size: 880 263 375 418 648 384 398 238 Cuts at: 4736 1132 Size: 3774 Fragments arranged by size: 3774 880 648 418 398 384 375 263 238 SspI AAT'ATT Cuts at: 5 52 5302 5 Size: 47 5250 2081 Fragments arranged by size: 5250 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7378 StyI C'CwwG_G Cuts at: 513 1065 2128 2304 2656 2895 3277 3599 3900 Size: 552 1063 176 352 239 382 322 301 Cuts at: 3900 3934 4030 4147 4179 4817 513 Size: 34 96 117 32 638 3074 Fragments arranged by size: 3074 1063 638 552 382 352 322 301 239 176 117 96 34 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2585 2888 5405 5422 5575 5760 6205 Size: 1255 1005 303 2517 17 153 185 445 Cuts at: 6205 325 Size: 1498 Fragments arranged by size: 2517 1498 1255 1005 445 303 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 2405 2694 Size: 274 80 33 51 1443 369 59 289 Cuts at: 2694 2817 5624 7336 96 Size: 123 2807 1712 138 Fragments arranged by size: 2807 1712 1443 369 289 274 138 123 80 59 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4143 4175 7338 7370 4143 Size: 32 3163 32 4151 Fragments arranged by size: 4151 3163 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4355 1316 Size: 3039 4339 XbaI T'CTAG_A Cuts at: 4714 4714 Size: 7378 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4361 4361 Size: 7378 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7378 XmaI C'CCGG_G Cuts at: 1870 4703 1870 Size: 2833 4545 XmnI GAAnn'nnTTC Cuts at: 2508 2937 5507 2508 Size: 429 2570 4379 Fragments arranged by size: 4379 2570 429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5186 276 Size: 53 83 186 936 3652 2468 Fragments arranged by size: 3652 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SfoI SrfI SwaI