Restriction map for
pFN21AA0698
(Linear) (Six-base) MAPSORT of: KIAA0698.seq Check: 8259 from: 1 to: 2673
KIAA0698.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1142 2673
Size: 1142 1531
Acc65I G'GTAC_C
Cuts at: 0 220 2591 2673
Size: 220 2371 82
Fragments arranged by size:
2371 220 82
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1183 2673
Size: 1183 1490
AflII C'TTAA_G
Cuts at: 0 2403 2673
Size: 2403 270
AflIII A'CryG_T
Cuts at: 0 47 63 1103 1871 2673
Size: 47 16 1040 768 802
Fragments arranged by size:
1040 802 768 47 16
AleI CACnn'nnGTG
Cuts at: 0 160 187 2673
Size: 160 27 2486
Fragments arranged by size:
2486 160 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1207 2104 2673
Size: 1207 897 569
Fragments arranged by size:
1207 897 569
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1156 2673
Size: 135 1021 1517
Fragments arranged by size:
1517 1021 135
ApaI G_GGCC'C
Cuts at: 0 865 1814 1895 2673
Size: 865 949 81 778
Fragments arranged by size:
949 865 778 81
ApaLI G'TGCA_C
Cuts at: 0 53 2673
Size: 53 2620
ApoI r'AATT_y
Cuts at: 0 30 411 846 928 1957 2673
Size: 30 381 435 82 1029 716
Fragments arranged by size:
1029 716 435 381 82 30
AseI AT'TA_AT
Cuts at: 0 1067 2673
Size: 1067 1606
BamHI G'GATC_C
Cuts at: 0 789 1583 1857 2037 2673
Size: 789 794 274 180 636
Fragments arranged by size:
794 789 636 274 180
BanI G'GyrC_C
Cuts at: 0 204 220 886 1130 2591 2673
Size: 204 16 666 244 1461 82
Fragments arranged by size:
1461 666 244 204 82 16
BanII G_rGCy'C
Cuts at: 0 460 865 1526 1814 1895 1928 2673
Size: 460 405 661 288 81 33 745
Fragments arranged by size:
745 661 460 405 288 81 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 603 2673
Size: 603 2070
BbvCI CC'TCA_GC
Cuts at: 0 584 2673
Size: 584 2089
BfrBI ATG'CAT
Cuts at: 0 3 534 1059 2091 2673
Size: 3 531 525 1032 582
Fragments arranged by size:
1032 582 531 525 3
BglI GCCn_nnn'nGGC
Cuts at: 0 860 1301 1913 2673
Size: 860 441 612 760
Fragments arranged by size:
860 760 612 441
BlpI GC'TnA_GC
Cuts at: 0 1754 2673
Size: 1754 919
Bme1580I G_kGCm'C
Cuts at: 0 57 209 478 865 1150 1814 1895 2450
Size: 57 152 269 387 285 664 81 555
Cuts at: 2450 2673
Size: 223
Fragments arranged by size:
664 555 387 285 269 223 152 81
57
BmrI ACTGGGnnnn_n'
Cuts at: 0 413 1341 2673
Size: 413 928 1332
Fragments arranged by size:
1332 928 413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 1942 2673
Size: 444 1498 731
Fragments arranged by size:
1498 731 444
Bpu10I CC'TnA_GC
Cuts at: 0 38 584 785 1507 2673
Size: 38 546 201 722 1166
Fragments arranged by size:
1166 722 546 201 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 2362 2673
Size: 2362 311
BsaBI GATnn'nnATC
Cuts at: 0 551 2189 2673
Size: 551 1638 484
Fragments arranged by size:
1638 551 484
BsaWI w'CCGG_w
Cuts at: 0 1445 2673
Size: 1445 1228
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 610 640 2138 2168 2673
Size: 610 30 1498 30 505
Fragments arranged by size:
1498 610 505 30 30
BseYI C'CCAG_C
Cuts at: 0 629 1451 2484 2673
Size: 629 822 1033 189
Fragments arranged by size:
1033 822 629 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 917 1213 1238 2673
Size: 897 20 296 25 1435
Fragments arranged by size:
1435 897 296 25 20
BsiHKAI G_wGCw'C
Cuts at: 0 57 460 2673
Size: 57 403 2213
Fragments arranged by size:
2213 403 57
Bsp1286I G_dGCh'C
Cuts at: 0 57 209 460 478 865 1150 1526 1814
Size: 57 152 251 18 387 285 376 288
Cuts at: 1814 1895 1928 2450 2673
Size: 81 33 522 223
Fragments arranged by size:
522 387 376 288 285 251 223 152
81 57 33 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 326 1097 1142 2673
Size: 326 771 45 1531
Fragments arranged by size:
1531 771 326 45
BsrDI GCAATG_nn'
Cuts at: 0 99 984 2478 2673
Size: 99 885 1494 195
Fragments arranged by size:
1494 885 195 99
BsrFI r'CCGG_y
Cuts at: 0 772 2673
Size: 772 1901
BsrGI T'GTAC_A
Cuts at: 0 689 2673
Size: 689 1984
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 1354 2673
Size: 606 748 1319
Fragments arranged by size:
1319 748 606
BstYI r'GATC_y
Cuts at: 0 789 828 1583 1838 1857 2037 2190 2265
Size: 789 39 755 255 19 180 153 75
Cuts at: 2265 2495 2673
Size: 230 178
Fragments arranged by size:
789 755 255 230 180 178 153 75
39 19
Bsu36I CC'TnA_GG
Cuts at: 0 2122 2673
Size: 2122 551
BtgI C'CryG_G
Cuts at: 0 123 144 299 651 1929 2025 2174 2673
Size: 123 21 155 352 1278 96 149 499
Fragments arranged by size:
1278 499 352 155 149 123 96 21
BtsI GCAGTG_nn'
Cuts at: 0 779 1859 2331 2450 2673
Size: 779 1080 472 119 223
Fragments arranged by size:
1080 779 472 223 119
EaeI y'GGCC_r
Cuts at: 0 1250 1356 1591 1741 2171 2303 2673
Size: 1250 106 235 150 430 132 370
Fragments arranged by size:
1250 430 370 235 150 132 106
EarI CTCTTCn'nnn_
Cuts at: 0 448 946 2380 2673
Size: 448 498 1434 293
Fragments arranged by size:
1434 498 448 293
EcoICRI GAG'CTC
Cuts at: 0 458 2673
Size: 458 2215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 1183 1942 2673
Size: 444 739 759 731
Fragments arranged by size:
759 739 731 444
EcoO109I rG'GnC_Cy
Cuts at: 0 1891 1892 2030 2673
Size: 1891 1 138 643
Fragments arranged by size:
1891 643 138 1
EcoRI G'AATT_C
Cuts at: 0 928 2673
Size: 928 1745
EcoRV GAT'ATC
Cuts at: 0 1659 2673
Size: 1659 1014
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 688 720 2673
Size: 688 32 1953
Fragments arranged by size:
1953 688 32
HindIII A'AGCT_T
Cuts at: 0 2653 2673
Size: 2653 20
KpnI G_GTAC'C
Cuts at: 0 224 2595 2673
Size: 224 2371 78
Fragments arranged by size:
2371 224 78
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 983 1035 2673
Size: 932 51 52 1638
Fragments arranged by size:
1638 932 52 51
MscI TGG'CCA
Cuts at: 0 1252 1358 1743 2173 2305 2673
Size: 1252 106 385 430 132 368
Fragments arranged by size:
1252 430 385 368 132 106
MslI CAynn'nnrTG
Cuts at: 0 158 160 187 604 808 1712 2210 2336
Size: 158 2 27 417 204 904 498 126
Cuts at: 2336 2354 2673
Size: 18 319
Fragments arranged by size:
904 498 417 319 204 158 126 27
18 2
MspA1I CmG'CkG
Cuts at: 0 752 867 994 2673
Size: 752 115 127 1679
Fragments arranged by size:
1679 752 127 115
NcoI C'CATG_G
Cuts at: 0 123 299 651 1929 2025 2174 2673
Size: 123 176 352 1278 96 149 499
Fragments arranged by size:
1278 499 352 176 149 123 96
NsiI A_TGCA'T
Cuts at: 0 5 536 1061 2093 2673
Size: 5 531 525 1032 580
Fragments arranged by size:
1032 580 531 525 5
NspI r_CATG'y
Cuts at: 0 7 51 270 645 1063 1107 1711 1875
Size: 7 44 219 375 418 44 604 164
Cuts at: 1875 2095 2169 2493 2673
Size: 220 74 324 180
Fragments arranged by size:
604 418 375 324 220 219 180 164
74 44 44 7
PasI CC'CwG_GG
Cuts at: 0 209 1807 2275 2314 2673
Size: 209 1598 468 39 359
Fragments arranged by size:
1598 468 359 209 39
PciI A'CATG_T
Cuts at: 0 47 1103 1871 2673
Size: 47 1056 768 802
Fragments arranged by size:
1056 802 768 47
PflMI CCAn_nnn'nTGG
Cuts at: 0 1600 2187 2673
Size: 1600 587 486
Fragments arranged by size:
1600 587 486
PfoI T'CCnGG_A
Cuts at: 0 1834 1919 2636 2673
Size: 1834 85 717 37
Fragments arranged by size:
1834 717 85 37
PpuMI rG'GwC_Cy
Cuts at: 0 2030 2673
Size: 2030 643
PspOMI G'GGCC_C
Cuts at: 0 861 1810 1891 2673
Size: 861 949 81 782
Fragments arranged by size:
949 861 782 81
PstI C_TGCA'G
Cuts at: 0 828 1854 2673
Size: 828 1026 819
Fragments arranged by size:
1026 828 819
PvuII CAG'CTG
Cuts at: 0 994 2673
Size: 994 1679
SacI G_AGCT'C
Cuts at: 0 460 2673
Size: 460 2213
SanDI GG'GwC_CC
Cuts at: 0 2030 2673
Size: 2030 643
SapI GCTCTTCn'nnn_
Cuts at: 0 448 2673
Size: 448 2225
ScaI AGT'ACT
Cuts at: 0 343 402 2673
Size: 343 59 2271
Fragments arranged by size:
2271 343 59
SexAI A'CCwGG_T
Cuts at: 0 216 223 2673
Size: 216 7 2450
Fragments arranged by size:
2450 216 7
SfcI C'TryA_G
Cuts at: 0 824 1850 2056 2673
Size: 824 1026 206 617
Fragments arranged by size:
1026 824 617 206
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 860 2673
Size: 860 1813
SmlI C'TyrA_G
Cuts at: 0 2403 2673
Size: 2403 270
SphI G_CATG'C
Cuts at: 0 7 270 645 1063 1711 2095 2493 2673
Size: 7 263 375 418 648 384 398 180
Fragments arranged by size:
648 418 398 384 375 263 180 7
StyI C'CwwG_G
Cuts at: 0 123 299 651 890 1272 1594 1895 1929
Size: 123 176 352 239 382 322 301 34
Cuts at: 1929 2025 2142 2174 2673
Size: 96 117 32 499
Fragments arranged by size:
499 382 352 322 301 239 176 123
117 96 34 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 580 883 2673
Size: 580 303 1790
Fragments arranged by size:
1790 580 303
TatI w'GTAC_w
Cuts at: 0 341 400 689 812 2673
Size: 341 59 289 123 1861
Fragments arranged by size:
1861 341 289 123 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2138 2170 2673
Size: 2138 32 503
Fragments arranged by size:
2138 503 32
Tth111I GACn'n_nGTC
Cuts at: 0 2350 2673
Size: 2350 323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2356 2673
Size: 2356 317
XmnI GAAnn'nnTTC
Cuts at: 0 503 932 2673
Size: 503 429 1741
Fragments arranged by size:
1741 503 429
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI BamHI BanI BanII BbsI
BbvCI BfrBI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BsaI BsaBI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
Hin4I HindIII KpnI MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PciI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SanDI SapI ScaI SexAI SfcI
SfiI SmlI SphI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AloI AscI
AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BclI
BglII BmgBI BmtI BplI BpuEI BsaAI BsaHI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI FalI FseI
FspI FspAI HaeII HincII HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SbfI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI
XmaI ZraI