Restriction map for
pFN21AE3825
##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccII AgeI AhdI AscI AseI
AsnI AspEI AviII AvrII BbvCI
BcgI BclI BcnI BglI BlnI
BsiEI BsiWI BspDI BsrFI BssHII
BstEII BstPI BstUI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRV FbaI
FseI FspI HpaI KspI MluI
MvnI NaeI NciI NgoMIV NotI
NruI PacI PinAI PmeI PshBI
PvuI RsrII SacII SbfI SfiI
SgrAI SmaI SnaBI Sse8387I SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3788 GACGTC GACGT^C
Acc65I 5199 GGTACC G^GTACC
AclI 1875 AACGTT AA^CGTT
AflII 7747 CTTAAG C^TTAAG
Alw44I 3014 GTGCAC G^TGCAC
Aor51HI 1079 AGCGCT AGC^GCT
ApaI 4574 GGGCCC GGGCC^C
ApaLI 3014 GTGCAC G^TGCAC
Asp718I 5199 GGTACC G^GTACC
BamHI 2959 GGATCC G^GATCC
BciVI 3508 GTATCC GTATCC(6/5)
BfrI 7747 CTTAAG C^TTAAG
BlpI 5017 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5017 GCTGAGC GC^T[ACGT]AGC
BsaBI 5077 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3424 GGTCTC GGTCTC(1/5)
BsrBI 5154 CCGCTC CCGCTC(-3/-3)
Bst1107I 3540 GTATAC GTA^TAC
BstZ17I 3540 GTATAC GTA^TAC
CelII 5017 GCTGAGC GC^T[ACGT]AGC
DsaI 4439 CCATGG C^C[GA][CT]GG
Eco47III 1079 AGCGCT AGC^GCT
FauI 5153 CCCGC CCCGC(4/6)
KpnI 5199 GGTACC GGTAC^C
MamI 5077 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 4439 CCATGG C^CATGG
NdeI 2723 CATATG CA^TATG
PflMI 1776 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1875 AACGTT AA^CGTT
SalI 2873 GTCGAC G^TCGAC
SapI 4526 GCTCTTC GCTCTTC(1/4)
ScaI 5005 AGTACT AGT^ACT
StuI 3846 AGGCCT AGG^CCT
Van91I 1776 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5376 TCTAGA T^CTAGA
XcmI 5003 CCAGTACTCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 5950 CCTCAGG CC^T[ACGT]AGG
7560 CCTGAGG
AspI 4824 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5342 GACCTGGTC
BbeI 6268 GGCGCC GGCGC^C
7109 GGCGCC
BbrPI 6407 CACGTG CAC^GTG
7541 CACGTG
BglII 1359 AGATCT A^GATCT
3417 AGATCT
BsaAI 6407 CACGTG [CT]AC^GT[GA]
7541 CACGTG
BspHI 6930 TCATGA T^CATGA
7778 TCATGA
Bsu36I 5950 CCTCAGG CC^T[ACGT]AGG
7560 CCTGAGG
DraIII 3017 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
6869 CACGAGGTG
Eco81I 5950 CCTCAGG CC^T[ACGT]AGG
7560 CCTGAGG
KasI 6268 GGCGCC G^GCGCC
7109 GGCGCC
NarI 6268 GGCGCC GG^CGCC
7109 GGCGCC
NheI 1842 GCTAGC G^CTAGC
7794 GCTAGC
PflFI 4824 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5342 GACCTGGTC
PmaCI 6407 CACGTG CAC^GTG
7541 CACGTG
PmlI 6407 CACGTG CAC^GTG
7541 CACGTG
PshAI 3556 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4948 GACACAGGTC
PvuII 3278 CAGCTG CAG^CTG
7271 CAGCTG
RcaI 6930 TCATGA T^CATGA
7778 TCATGA
SfoI 6268 GGCGCC GGC^GCC
7109 GGCGCC
SpeI 1725 ACTAGT A^CTAGT
7170 ACTAGT
Tth111I 4824 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5342 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccIII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspEI BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BssSI BstAPI BstBI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI NspV PaeR7I
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfuI SmlI SphI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoI XhoII XmnI