(Circular) (Six-base) MAPSORT of: pFN21AE3825.seq Check: 3491 from: 1 to: 12535 pFN21AE3825 12535 bp With 182 enzymes: SgfI * August 14, 2013 14:06 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5797 10345 278 Size: 53 83 186 936 4261 4548 2468 Fragments arranged by size: 4548 4261 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4879 4936 5546 9878 1937 Size: 2942 57 610 4332 4594 Fragments arranged by size: 4594 4332 2942 610 57 Acc65I G'GTAC_C Cuts at: 7204 9856 7204 Size: 2652 9883 AclI AA'CG_TT Cuts at: 1563 3881 10662 11035 1563 Size: 2318 6781 373 3063 Fragments arranged by size: 6781 3063 2318 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3425 3693 6519 7991 8934 9165 9203 9430 Size: 1793 268 2826 1472 943 231 38 227 Cuts at: 9430 10598 11982 12250 1632 Size: 1168 1384 268 1917 Fragments arranged by size: 2826 1917 1793 1472 1384 1168 943 268 268 231 227 38 AfeI AGC'GCT Cuts at: 3086 3086 Size: 12535 AflII C'TTAA_G Cuts at: 820 1017 9752 820 Size: 197 8735 3603 Fragments arranged by size: 8735 3603 197 AflIII A'CryG_T Cuts at: 1203 2144 4286 5638 8258 9545 9737 11434 12411 Size: 941 2142 1352 2620 1287 192 1697 977 Cuts at: 12411 1203 Size: 1327 Fragments arranged by size: 2620 2142 1697 1352 1327 1287 977 941 192 AgeI A'CCGG_T Cuts at: 11366 11366 Size: 12535 AhdI GACnn_n'nnGTC Cuts at: 11264 11264 Size: 12535 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 12535 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 7779 8475 1184 Size: 6595 696 5244 Fragments arranged by size: 6595 5244 696 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2460 2492 2460 Size: 32 12503 AlwNI CAG_nnn'CTG Cuts at: 5815 6263 6488 7772 8014 9200 10278 11850 5815 Size: 448 225 1284 242 1186 1078 1572 6500 Fragments arranged by size: 6500 1572 1284 1186 1078 448 242 225 ApaI G_GGCC'C Cuts at: 6583 6583 Size: 12535 ApaLI G'TGCA_C Cuts at: 5019 10592 11748 5019 Size: 5573 1156 5806 Fragments arranged by size: 5806 5573 1156 ApoI r'AATT_y Cuts at: 1493 2181 2559 3172 3242 4172 4430 5188 5990 Size: 688 378 613 70 930 258 758 802 Cuts at: 5990 8108 8489 8555 8751 8981 9030 9120 9235 Size: 2118 381 66 196 230 49 90 115 Cuts at: 9235 9844 10070 10222 12331 1493 Size: 609 226 152 2109 1697 Fragments arranged by size: 2118 2109 1697 930 802 758 688 613 609 381 378 258 230 226 196 152 115 90 70 66 49 AseI AT'TA_AT Cuts at: 160 1032 11089 160 Size: 872 10057 1606 Fragments arranged by size: 10057 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12535 AvaI C'yCGr_G Cuts at: 1870 1943 3405 4197 4515 9860 1870 Size: 73 1462 792 318 5345 4545 Fragments arranged by size: 5345 4545 1462 792 318 73 BamHI G'GATC_C Cuts at: 4964 9865 10232 4964 Size: 4901 367 7267 Fragments arranged by size: 7267 4901 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3553 5202 7204 8273 9114 Size: 325 217 627 1766 1649 2002 1069 841 Cuts at: 9114 9856 11311 618 Size: 742 1455 1842 Fragments arranged by size: 2002 1842 1766 1649 1455 1069 841 742 627 325 217 BanII G_rGCy'C Cuts at: 1391 2623 2794 3534 4198 5839 6583 6593 7029 Size: 1232 171 740 664 1641 744 10 436 Cuts at: 7029 7419 7614 8182 9854 1391 Size: 390 195 568 1672 4072 Fragments arranged by size: 4072 1672 1641 1232 744 740 664 568 436 390 195 171 10 BbeI G_GCGC'C Cuts at: 8277 9118 8277 Size: 841 11694 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3325 4679 5355 9051 9588 928 Size: 971 1426 1354 676 3696 537 3875 Fragments arranged by size: 3875 3696 1426 1354 971 676 537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10726 10760 1903 Size: 34 8789 34 3678 Fragments arranged by size: 8789 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5524 10427 11637 5524 Size: 4903 1210 6422 Fragments arranged by size: 6422 4903 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 12535 BfrBI ATG'CAT Cuts at: 2100 8769 9367 2100 Size: 6669 598 5268 Fragments arranged by size: 6669 5268 598 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 11146 136 Size: 107 122 71 631 605 9474 1525 Fragments arranged by size: 9474 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3364 5422 12530 3364 Size: 2058 7108 3369 Fragments arranged by size: 7108 3369 2058 BlpI GC'TnA_GC Cuts at: 7023 9952 7023 Size: 2929 9606 Bme1580I G_kGCm'C Cuts at: 1790 5023 6583 10596 11752 1790 Size: 3233 1560 4013 1156 2573 Fragments arranged by size: 4013 3233 2573 1560 1156 BmgBI CAC'GTC Cuts at: 1318 8309 1318 Size: 6991 5544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3217 10266 11224 Size: 416 53 83 399 24 1800 7049 958 Cuts at: 11224 442 Size: 1753 Fragments arranged by size: 7049 1800 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3851 9803 1056 Size: 2795 5952 3788 Fragments arranged by size: 5952 3788 2795 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2187 3432 4964 7233 8385 11195 1239 Size: 948 1245 1532 2269 1152 2810 2579 Fragments arranged by size: 2810 2579 2269 1532 1245 1152 948 Bpu10I CC'TnA_GC Cuts at: 7050 9531 7050 Size: 2481 10054 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2526 3309 5984 6124 6604 8263 8372 8501 9241 Size: 783 2675 140 480 1659 109 129 740 Cuts at: 9241 9272 10660 11525 11823 2526 Size: 31 1388 865 298 3238 Fragments arranged by size: 3238 2675 1659 1388 865 783 740 480 298 140 129 109 31 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5435 11198 882 Size: 617 3936 5763 2219 Fragments arranged by size: 5763 3936 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 8414 9548 10326 493 Size: 711 7210 1134 778 2702 Fragments arranged by size: 7210 2702 1134 778 711 BsaBI GATnn'nnATC Cuts at: 7086 12529 7086 Size: 5443 7092 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5794 7129 7273 8274 Size: 53 83 186 936 4261 1335 144 1001 Cuts at: 8274 9115 10342 10724 11359 275 Size: 841 1227 382 635 1451 Fragments arranged by size: 4261 1451 1335 1227 1001 936 841 635 382 186 144 83 53 BsaWI w'CCGG_w Cuts at: 2875 3060 3544 4135 10968 11366 11640 11787 12198 Size: 185 484 591 6833 398 274 147 411 Cuts at: 12198 2875 Size: 3212 Fragments arranged by size: 6833 3212 591 484 411 398 274 185 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2134 2164 2776 2806 4557 Size: 30 165 30 731 30 612 30 1751 Cuts at: 4557 4587 5826 5856 6252 6282 1178 Size: 30 1239 30 396 30 7431 Fragments arranged by size: 7431 1751 1239 731 612 396 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2142 2720 6127 6759 7124 9791 1186 Size: 956 578 3407 632 365 2667 3930 Fragments arranged by size: 3930 3407 2667 956 632 578 365 BseYI C'CCAG_C Cuts at: 2674 6575 9013 9535 11738 12234 2674 Size: 3901 2438 522 2203 496 2975 Fragments arranged by size: 3901 2975 2438 2203 522 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3282 5472 8684 1163 Size: 573 1546 2190 3212 5014 Fragments arranged by size: 5014 3212 2190 1546 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9985 10746 11774 1540 Size: 275 186 7984 761 1028 2301 Fragments arranged by size: 7984 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2203 2623 2794 4198 5023 5839 6593 6758 6784 Size: 420 171 1404 825 816 754 165 26 Cuts at: 6784 7029 7419 10596 10681 11752 2203 Size: 245 390 3177 85 1071 2986 Fragments arranged by size: 3177 2986 1404 1071 825 816 754 420 390 245 171 165 85 26 BsmI GAATG_Cn' Cuts at: 5410 7768 8769 9614 10046 10139 5410 Size: 2358 1001 845 432 93 7806 Fragments arranged by size: 7806 2358 1001 845 432 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5785 7120 8390 5785 Size: 1335 1270 9930 Fragments arranged by size: 9930 1335 1270 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2203 2623 2794 3534 4198 5023 5839 Size: 399 413 420 171 740 664 825 816 Cuts at: 5839 6583 6593 6758 6784 7029 7419 7614 8182 Size: 744 10 165 26 245 390 195 568 Cuts at: 8182 9854 10596 10681 11752 1391 Size: 1672 742 85 1071 2174 Fragments arranged by size: 2174 1672 1071 825 816 744 742 740 664 568 420 413 399 390 245 195 171 165 85 26 10 BspEI T'CCGG_A Cuts at: 3060 3544 4135 12198 3060 Size: 484 591 8063 3397 Fragments arranged by size: 8063 3397 591 484 BspHI T'CATG_A Cuts at: 8935 9783 10424 12154 8935 Size: 848 641 1730 9316 Fragments arranged by size: 9316 1730 848 641 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3878 7216 7761 9678 9890 844 Size: 3034 3338 545 1917 212 3489 Fragments arranged by size: 3489 3338 3034 1917 545 212 BsrBI CCG'CTC Cuts at: 1394 1823 7161 10422 1394 Size: 429 5338 3261 3507 Fragments arranged by size: 5338 3507 3261 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2027 2930 5398 6868 7582 7978 11030 Size: 1183 779 903 2468 1470 714 396 3052 Cuts at: 11030 11204 65 Size: 174 1396 Fragments arranged by size: 3052 2468 1470 1396 1183 903 779 714 396 174 BsrFI r'CCGG_y Cuts at: 1700 1810 11179 11366 1700 Size: 110 9369 187 2869 Fragments arranged by size: 9369 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 7543 7629 8261 96 Size: 7447 86 632 4370 Fragments arranged by size: 7447 4370 632 86 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12535 BssSI C'ACGA_G Cuts at: 2400 4560 5469 5838 7491 8874 10595 11607 2400 Size: 2160 909 369 1653 1383 1721 1012 3328 Fragments arranged by size: 3328 2160 1721 1653 1383 1012 909 369 BstAPI GCAn_nnn'nTGC Cuts at: 794 3610 3638 5261 7772 794 Size: 2816 28 1623 2511 5557 Fragments arranged by size: 5557 2816 2511 1623 28 BstBI TT'CG_AA Cuts at: 3115 3649 4900 5167 5706 11339 3115 Size: 534 1251 267 539 5633 4311 Fragments arranged by size: 5633 4311 1251 539 534 267 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 12535 BstXI CCAn_nnnn'nTGG Cuts at: 3456 3990 5425 8069 3456 Size: 534 1435 2644 7922 Fragments arranged by size: 7922 2644 1435 534 BstYI r'GATC_y Cuts at: 1972 3035 3364 3541 4145 4964 5422 9283 9865 Size: 1063 329 177 604 819 458 3861 582 Cuts at: 9865 10232 10617 10634 12086 12172 12184 12530 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 3861 1977 1452 1063 819 604 582 458 385 367 346 329 177 86 17 12 BstZ17I GTA'TAC Cuts at: 5547 5547 Size: 12535 Bsu36I CC'TnA_GG Cuts at: 7956 9566 7956 Size: 1610 10925 BtgI C'CryG_G Cuts at: 513 1065 2004 6444 513 Size: 552 939 4440 6604 Fragments arranged by size: 6604 4440 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2641 487 Size: 683 56 1415 10381 Fragments arranged by size: 10381 1415 683 56 BtsI GCAGTG_nn' Cuts at: 2802 3741 4750 6721 8350 10051 10845 10865 2802 Size: 939 1009 1971 1629 1701 794 20 4472 Fragments arranged by size: 4472 1971 1701 1629 1009 939 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12535 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3714 3749 9277 9312 529 Size: 35 3150 35 5528 35 3752 Fragments arranged by size: 5528 3752 3150 35 35 35 DraI TTT'AAA Cuts at: 4540 4843 5379 8818 9084 9234 9839 4540 Size: 303 536 3439 266 150 605 7236 Fragments arranged by size: 7236 3439 605 536 303 266 150 DraIII CAC_nnn'GTG Cuts at: 5027 8879 5027 Size: 3852 8683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 11542 809 Size: 507 10226 1802 Fragments arranged by size: 10226 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3778 5338 6177 Size: 54 1425 50 275 18 1948 1560 839 Cuts at: 6177 8760 9982 10871 12500 8 Size: 2583 1222 889 1629 43 Fragments arranged by size: 2583 1948 1629 1560 1425 1222 889 839 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9982 1537 Size: 275 8170 4090 Fragments arranged by size: 8170 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3909 6538 7245 7920 8958 10465 1353 Size: 2556 2629 707 675 1038 1507 3423 Fragments arranged by size: 3423 2629 2556 1507 1038 707 675 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 11122 11496 11642 387 Size: 1064 138 9533 374 146 1280 Fragments arranged by size: 9533 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2621 2792 4196 5837 6591 7027 7417 2621 Size: 171 1404 1641 754 436 390 7739 Fragments arranged by size: 7739 1641 1404 754 436 390 171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 3425 3432 3693 4964 6519 7233 Size: 393 555 1238 7 261 1271 1555 714 Cuts at: 7233 7991 8385 8934 9165 9203 9430 10598 11195 Size: 758 394 549 231 38 227 1168 597 Cuts at: 11195 11982 12250 1239 Size: 787 268 1524 Fragments arranged by size: 1555 1524 1271 1238 1168 787 758 714 597 555 549 394 393 268 261 231 227 38 7 EcoO109I rG'GnC_Cy Cuts at: 3890 4250 6398 6579 6580 7401 8519 3890 Size: 360 2148 181 1 821 1118 7906 Fragments arranged by size: 7906 2148 1118 821 360 181 1 EcoRI G'AATT_C Cuts at: 4172 8108 8555 9844 10222 4172 Size: 3936 447 1289 378 6485 Fragments arranged by size: 6485 3936 1289 447 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3653 4781 5795 6930 7756 8021 8061 3653 Size: 1128 1014 1135 826 265 40 8127 Fragments arranged by size: 8127 1135 1128 1014 826 265 40 FspI TGC'GCA Cuts at: 11041 11041 Size: 12535 HaeII r_GCGC'y Cuts at: 1678 3088 8277 9118 9796 11682 1678 Size: 1410 5189 841 678 1886 2531 Fragments arranged by size: 5189 2531 1886 1410 841 678 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2383 2415 2699 2731 6104 Size: 32 135 32 811 32 284 32 3373 Cuts at: 6104 6136 6252 6284 7363 7395 7443 7475 7515 Size: 32 116 32 1079 32 48 32 40 Cuts at: 7515 7547 8456 8488 8597 8629 8930 8960 8962 Size: 32 909 32 109 32 301 30 2 Cuts at: 8962 8992 11176 11208 11250 11282 1373 Size: 30 2184 32 42 32 2626 Fragments arranged by size: 3373 2626 2184 1079 909 811 301 284 135 116 109 48 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 2671 2835 2977 3879 4229 4817 Size: 988 281 733 164 142 902 350 588 Cuts at: 4817 4880 5586 6837 9879 10125 669 Size: 63 706 1251 3042 246 3079 Fragments arranged by size: 3079 3042 1251 988 902 733 706 588 350 281 246 164 142 63 HindIII A'AGCT_T Cuts at: 748 3012 6002 8916 8956 748 Size: 2264 2990 2914 40 4327 Fragments arranged by size: 4327 2990 2914 2264 40 HpaI GTT'AAC Cuts at: 10125 10125 Size: 12535 KasI G'GCGC_C Cuts at: 8273 9114 8273 Size: 841 11694 KpnI G_GTAC'C Cuts at: 7208 9860 7208 Size: 2652 9883 MfeI C'AATT_G Cuts at: 2838 2919 3147 6279 7559 10134 2838 Size: 81 228 3132 1280 2575 5239 Fragments arranged by size: 5239 3132 2575 1280 228 81 MluI A'CGCG_T Cuts at: 12411 12411 Size: 12535 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2883 4992 11649 11833 1305 Size: 1578 2109 6657 184 2007 Fragments arranged by size: 6657 2109 2007 1578 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3780 6179 8762 10 Size: 54 1425 343 1948 2399 2583 3783 Fragments arranged by size: 3783 2583 2399 1948 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2761 4285 4950 5208 5391 5469 5556 Size: 648 1595 1524 665 258 183 78 87 Cuts at: 5556 7625 9831 10493 10852 11011 518 Size: 2069 2206 662 359 159 2042 Fragments arranged by size: 2206 2069 2042 1595 1524 665 662 648 359 258 183 159 87 78 MspA1I CmG'CkG Cuts at: 1606 5285 6362 9278 9951 10628 11776 12021 1606 Size: 3679 1077 2916 673 677 1148 245 2120 Fragments arranged by size: 3679 2916 2120 1148 1077 677 673 245 NarI GG'CG_CC Cuts at: 8274 9115 8274 Size: 841 11694 NcoI C'CATG_G Cuts at: 513 1065 2004 6444 513 Size: 552 939 4440 6604 Fragments arranged by size: 6604 4440 939 552 NdeI CA'TA_TG Cuts at: 387 4729 387 Size: 4342 8193 NheI G'CTAG_C Cuts at: 1052 3847 9799 1052 Size: 2795 5952 3788 Fragments arranged by size: 5952 3788 2795 NotI GC'GGCC_GC Cuts at: 9982 9982 Size: 12535 NsiI A_TGCA'T Cuts at: 2102 8771 9369 2102 Size: 6669 598 5268 Fragments arranged by size: 6669 5268 598 NspI r_CATG'y Cuts at: 1132 1229 2074 2104 2148 2766 3312 4290 4558 Size: 97 845 30 44 618 546 978 268 Cuts at: 4558 5642 7700 8262 8462 9595 9741 9893 11438 Size: 1084 2058 562 200 1133 146 152 1545 Cuts at: 11438 1132 Size: 2229 Fragments arranged by size: 2229 2058 1545 1133 1084 978 845 618 562 546 268 200 152 146 97 44 30 PasI CC'CwG_GG Cuts at: 1348 4420 7397 1348 Size: 3072 2977 6486 Fragments arranged by size: 6486 3072 2977 PciI A'CATG_T Cuts at: 2144 4286 5638 8258 9737 11434 2144 Size: 2142 1352 2620 1479 1697 3245 Fragments arranged by size: 3245 2620 2142 1697 1479 1352 PflMI CCAn_nnn'nTGG Cuts at: 1108 3787 1108 Size: 2679 9856 PfoI T'CCnGG_A Cuts at: 3753 5830 8650 3753 Size: 2077 2820 7638 Fragments arranged by size: 7638 2820 2077 PmeI GTTT'AAAC Cuts at: 9839 9839 Size: 12535 PmlI CAC'GTG Cuts at: 8414 9548 8414 Size: 1134 11401 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4498 4530 8391 8423 10604 10636 12117 12149 4498 Size: 32 3861 32 2181 32 1481 32 4884 Fragments arranged by size: 4884 3861 2181 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3890 4250 7401 8519 3890 Size: 360 3151 1118 7906 Fragments arranged by size: 7906 3151 1118 360 PshAI GACnn'nnGTC Cuts at: 1613 5565 6957 1613 Size: 3952 1392 7191 Fragments arranged by size: 7191 3952 1392 PsiI TTA'TAA Cuts at: 10105 10105 Size: 12535 PspOMI G'GGCC_C Cuts at: 6579 6579 Size: 12535 PspXI vC'TCGA_Gb Cuts at: 4197 4197 Size: 12535 PstI C_TGCA'G Cuts at: 830 2809 4411 5567 6133 7758 9887 830 Size: 1979 1602 1156 566 1625 2129 3478 Fragments arranged by size: 3478 2129 1979 1625 1602 1156 566 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12535 PvuII CAG'CTG Cuts at: 5285 9278 5285 Size: 3993 8542 SacI G_AGCT'C Cuts at: 2623 2794 4198 5839 6593 7029 7419 2623 Size: 171 1404 1641 754 436 390 7739 Fragments arranged by size: 7739 1641 1404 754 436 390 171 SalI G'TCGA_C Cuts at: 1936 4878 9877 1936 Size: 2942 4999 4594 Fragments arranged by size: 4999 4594 2942 SanDI GG'GwC_CC Cuts at: 7401 8519 7401 Size: 1118 11417 SapI GCTCTTCn'nnn_ Cuts at: 6538 6538 Size: 12535 SbfI CC_TGCA'GG Cuts at: 9887 9887 Size: 12535 ScaI AGT'ACT Cuts at: 7012 10783 12495 7012 Size: 3771 1712 7052 Fragments arranged by size: 7052 3771 1712 SexAI A'CCwGG_T Cuts at: 2770 4360 5023 6808 7348 2770 Size: 1590 663 1785 540 7957 Fragments arranged by size: 7957 1785 1590 663 540 SfcI C'TryA_G Cuts at: 826 1045 2042 2805 3039 4266 4407 5082 5563 Size: 219 997 763 234 1227 141 675 481 Cuts at: 5563 6129 6870 7689 7754 8922 9883 11018 11699 Size: 566 741 819 65 1168 961 1135 681 Cuts at: 11699 11890 826 Size: 191 1471 Fragments arranged by size: 1471 1227 1168 1135 997 961 819 763 741 681 675 566 481 234 219 191 141 65 SfoI GGC'GCC Cuts at: 8275 9116 8275 Size: 841 11694 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12535 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12535 SmaI CCC'GGG Cuts at: 1872 9862 1872 Size: 7990 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2541 3324 3405 4197 4515 5999 Size: 197 926 598 783 81 792 318 1484 Cuts at: 5999 6139 6619 8278 8387 8480 9256 9287 9752 Size: 140 480 1659 109 93 776 31 465 Cuts at: 9752 10639 11540 11802 820 Size: 887 901 262 1553 Fragments arranged by size: 1659 1553 1484 926 901 887 792 783 776 598 480 465 318 262 197 140 109 93 81 31 SnaBI TAC'GTA Cuts at: 493 10326 493 Size: 9833 2702 SpeI A'CTAG_T Cuts at: 152 3730 9175 152 Size: 3578 5445 3512 Fragments arranged by size: 5445 3578 3512 SphI G_CATG'C Cuts at: 1132 2104 2766 4558 9893 1132 Size: 972 662 1792 5335 3774 Fragments arranged by size: 5335 3774 1792 972 662 SspI AAT'ATT Cuts at: 5 52 4344 6974 8139 9767 10459 5 Size: 47 4292 2630 1165 1628 692 2081 Fragments arranged by size: 4292 2630 2081 1628 1165 692 47 StuI AGG'CCT Cuts at: 1517 5853 1517 Size: 4336 8199 StyI C'CwwG_G Cuts at: 513 1065 2004 4053 6444 7711 8746 9974 513 Size: 552 939 2049 2391 1267 1035 1228 3074 Fragments arranged by size: 3074 2391 2049 1267 1228 1035 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2664 3571 5160 5425 6055 6767 8822 Size: 1255 1084 907 1589 265 630 712 2055 Cuts at: 8822 10562 10579 10732 10917 11362 325 Size: 1740 17 153 185 445 1498 Fragments arranged by size: 2055 1740 1589 1498 1255 1084 907 712 630 445 265 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2396 4696 4915 Size: 274 80 33 51 1443 419 2300 219 Cuts at: 4915 7010 7436 7543 7629 8261 8897 10781 12493 Size: 2095 426 107 86 632 636 1884 1712 Cuts at: 12493 96 Size: 138 Fragments arranged by size: 2300 2095 1884 1712 1443 636 632 426 419 274 219 138 107 86 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2776 2808 8646 8678 12495 12527 2776 Size: 32 5838 32 3817 32 2784 Fragments arranged by size: 5838 3817 2784 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6832 7350 1316 Size: 5516 518 6501 Fragments arranged by size: 6501 5516 518 XbaI T'CTAG_A Cuts at: 7381 9871 7381 Size: 2490 10045 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 7015 2011 Size: 5004 7531 XhoI C'TCGA_G Cuts at: 1943 3405 4197 4515 1943 Size: 1462 792 318 9963 Fragments arranged by size: 9963 1462 792 318 XmaI C'CCGG_G Cuts at: 1870 9860 1870 Size: 7990 4545 XmnI GAAnn'nnTTC Cuts at: 7834 8985 9614 10664 7834 Size: 1151 629 1050 9705 Fragments arranged by size: 9705 1151 1050 629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5795 10343 276 Size: 53 83 186 936 4261 4548 2468 Fragments arranged by size: 4548 4261 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BplI BsiWI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PsrI RsrII SacII SfiI SrfI SwaI